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Fast cross-linking by DOPA2 promotes the capturing of a stereospecific protein complex over nonspecific encounter complexes

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Biophysics Reports 2022年第Z1期8卷 239-252页

作者： jian-Hua Wang Zhou Gong xu Dong Shu-Qun Liu Yu-Liang Tang Xiaoguang Lei Chun Tang Meng-Qiu Dong National institute of Biological sciences (NIBS) Tsinghua institute of Multidisciplinary Biomedical Research Tsinghua University state key laboratory of Magnetic Resonance and Atomic {3. physics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences state key laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan University beijing National laboratory for molecular {3. College of Chemistry and Molecular EngineeringCenter for Quantitative BiologyPeking-Tsinghua Center for Life SciencePeking University

Transient and weak protein–protein interactions are essential to many biochemical reactions, yet are technically challenging to study. Chemical cross-linking of proteins coupled with mass spectrometry analysis(CXMS) provides a powerful tool in the analysis of such interactions. Central to this technology are chemical cross-linkers. Here, using two transient heterodimeric complexes EIN/HPr and EIIAGlc/EIIBGlc as our model systems, we evaluated the effects of two amine-specific homo-bifunctional cross-linkers with different reactivities. We showed previously that DOPA2(di-ortho-phthalaldehyde with a di-ethylene glycol spacer arm) cross-links proteins 60–120 times faster than DSS(disuccinimidyl suberate). We found that though most of the intermolecular cross-links of either cross-linker are consistent with the encounter complexes(ECs), an ensemble of short-lived binding intermediates,more DOPA2 intermolecular cross-links could be assigned to the stereospecific complex(SC), the final lowest-energy conformational state for the two interacting proteins. Our finding suggests that faster cross-linking captures the SC more effectively and cross-linkers of different reactivities potentially probe protein–protein interaction dynamics across multiple timescales.

关键词： Mass spectrometry Chemical cross-linking Cross-linker Transient protein–protein interaction Encounter complexes Stereospecific complex DOPA2 DSS

Biofilm-inspired adhesive and antibacterial hydrogel with tough tissue integration performance for sealing hemostasis and wound healing

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Bioactive Materials 2020年第4期5卷 768-778页

作者： Wei Han Bo Zhou Kai Yang Xin Xiong Shifang Luan Yu Wang Zheng xu Peng Lei Zhengshan Luo jian Gao Yijing Zhan Guopu Chen Lei Liang Rui Wang Sha Li Hong xu state key laboratory of Materials-Oriented Chemical Engineering College of Food Science and Light IndustryNanjing Tech UniversityNanjing211816PR China Department of Gastrointestinal Surgery Department of General SurgeryThe First Affiliated Hospital of Anhui Medical University218 JiXi AvenueHefei230022AnhuiPR China Natural and Medical sciences institute at the University of Tübingen Markwiesenstr.5572770ReutlingenGermany state key laboratory of {3. physics and chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022PR China Department of Comparative Medicine Jinling HospitalNo.305 Zhongshan East RoadNanjing210002PR China of Marine and Bioengineering Yancheng Institute of TechnologyYancheng224051PR China

Uncontrolled bleeding and infection can cause significant increases in *** sealants have attracted extensive attention for their ability to control ***,because interfacial water is a formidable barrier to strong surface bonding,a challenge remains in finding a product that offers robust tissue adhesion combined with anti-infection *** by the strong adhesive mechanism of biofilm and mussels,we report a novel dual bionic adhesive hydrogel(DBAH)based on chitosan grafted with methacrylate(CS-MA),dopamine(DA),and N-hydroxymethyl acrylamide(NMA)via a facile radical polymerization ***-MA and DA were simultaneously included in the adhesive polymer for imitating the two key adhesive components:polysaccharide intercellular adhesin(PIA)of staphylococci biofilm and 3.4-dihydroxy-L-phenylalanine(Dopa)of mussel foot protein,*** presented strong adhesion at 3. kPa even upon three cycles of full immersion in water and was able to withstand up to 168 mm Hg blood pressure,which is significantly higher than the 60–160 mm Hg measured in most clinical *** importantly,these hydrogels presented outstanding hemostatic capability under wet and dynamic in vivo movements while displaying excellent antibacterial properties and ***,DBAH represents a promising class of biomaterials for high-efficiency hemostasis and wound healing.

关键词： Biofilm Mussel Dual-biomimetic Adhesive Hydrogel Hemostasis Antibacterial

Two-dimensional electron gas characteristics of InP-based high electron mobility transistor terahertz detector

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chinese physics B 2018年第4期27卷 363-368页

作者： Jin-Lun Li Shao-Hui Cui jian-Xing xu Xiao-Ran Cui Chun-Yan Guo Ben Ma Hai-Qiao Ni Zhi-Chuan Niu Department of Missile Engineering Shijiazhuang Campus Army Engineering University Shijiazhuang 050003 China state key laboratory for Superlattices Institute of Semiconductors Chinese Academy of Sciences （CAS） Beijing 100083 China College of Materials science and Opto-Electronic Technology University of Chinese Academy of Sciences Beijing 100049 China Microsystem ＆ Terahertz Research center China Academy of Engineering Physics Chengdu 610200 China Wide Bandgap Semiconductor Technology Disciplines state key laboratory Xidian University Xi＇an 710071 China key laboratory of Ultra-fast Photoelectric Diagnostics Technology of chinese academy of sciences Xi＇ an Institute of Optics and Precision Mechanics Xi＇ an 710119 China

The samples of InxGa（1-x）As/In（0.52）Al（0.48）As two-dimensional electron gas（2DEG）are grown by molecular beam epitaxy（MBE）.In the sample preparation process,the In content and spacer layer thickness are changed and two kinds of methods,i.e.,contrast body doping andδ-doping are *** samples are analyzed by the Hall measurements at 3.0 Kand 77 *** InxGa1-xAs/In0.52Al0.48As 2DEG channel structures with mobilities as high as 10289 cm^2/V·s（3.0 K）and42040 cm^2/V·s（77 K）are obtained,and the values of carrier concentration（Nc）are 3.465×10^12/cm^2 and 2.502×10^12/cm^2,*** THz response rates of In P-based high electron mobility transistor（HEMT）structures with different gate lengths at 3.0 K and 77 K temperatures are calculated based on the shallow water wave instability *** results provide a reference for the research and preparation of In P-based HEMT THz detectors.

关键词： THz detector high electron mobility transistor two-dimensional electron gas InP

Wet-adhesive materials of oral and maxillofacial region:From design to application

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chinese Chemical Letters 2023年第1期34卷 5-18页

作者： Yilin Mao Zhengyi xu Zihan He jian Wang Zhou Zhu state key laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China West china School of Stomatology Sichuan UniversityChengdu 610041China

Oral and maxillofacial diseases are a group of high-incidence disorders that affect people’s life quality to a great extent,while the wet and highly movable environment of the related regions brings challenges to traditional *** with the obstacles of insufficient adhesive strength and ensuing short drug retention time,conventional oral therapeutic agents often have difficulty in achieving their desired *** and maxillofacial wet-adhesive materials have the advantages of excellent wet environment retention,internal stability,plasticity,and clinical potential,thus have become a significant research direction in the field of oral related disorders *** the past decade,the development of oral adhesive materials with good wet adhesion has accelerated based on the chemical molecular interaction,physical interlocking,and biological adhesion mechanisms,including biomimetic-inspired materials,naturally derived polymer–based materials and adhesive electrospun fiber *** fancy wet-adhesive materials can be used for oral mucosal drug delivery,oral vaccination,wound healing,and bone defects *** their numerous novel applications,wet-adhesive materials in stomatology still face unresolved challenges from material and biological ***,advances in designs of oral and maxillofacial wetadhesive materials are reviewed in terms of design backgrounds,attachment mechanisms,and common *** demonstrations of wet-adhesive materials for oral and maxillofacial region medical applications from drug delivery to multifunctional tissue treatments are *** conclude,current challenges and prospects on potential applications of oral and maxillofacial wet-adhesive materials are also briefly discussed.

关键词： Oral cavity Oral and maxillofacial region Wet adhesion Stomatology Adhesive materials Mucoadhesion Tissue engineering

Ultrafast Magnetization Precession in Perpendicularly Magnetized L10-MnAl Thin Films with Co2 MnSi Buffer Layers

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chinese physics Letters 2020年第5期37卷 110-114页

作者： Si-Wei Mao Jun Lu Long Yang xue-Zhong Ruan Hai-Long Wang Da-Hai Wei Yong-Bing xu jian-Hua Zhao state key laboratory of Superlattices and Microstructures Institute of SemiconductorsChinese Academy of SciencesBeijing 100083 center of Materials science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100190 beijing academy of Quantum Information science Beijing 100193 jiangsu Provincial key laboratory of Advanced Photonic and Electronic Materials Collaborative Innovation Center of Advanced MicrostructuresSchool of Electronic Science and EngineeringNanjing UniversityNanjing 210093

Perpendicularly magnetized L10-MnAl thin films with Co2 MnSi bufer layers were prepared on GaAs(001)substrates by molecular-beam epitaxy(MBE).The samples with high crystalline quality show a maximum uniaxial perpendicular magnetic anisotropy constant of 1.4×10^7 erg/cm^*** spin dynamics with a magnetization precession frequency up to 200 GHz was investigated by using time-resolved magneto-optical Kerr effect(TRMOKE)measurements,from which the Gilbert damping constantαof epitaxial L10-MnAl thin films is evaluated to be less than *** work provides an important reference for analyzing the current-induced magnetization switching process in MnAl-based spintronic devices.

关键词： magnetization Gilbert anisotropy

A ternary organic solar cell with 3.0 nm thick active layer shows over 14% efficiency

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science china chemistry 2020年第1期63卷 21-27页

作者： Lijiao Ma Ye xu Yunfei Zu Qing Liao Bowei xu Cunbin An Shaoqing Zhang jianhui Hou state key laboratory of {3. physics and chemistry Beijing National Laboratory for MolecularSciences CAS Research/Education Center for Excellence in Molecular SciencesInstitute of Chemistry Chinese Academy of SciencesBeijing 100190China School of chemistry and Biology Engineering University of Science and Technology BeijingBeijing 100083China University of chinese academy of sciences Beijing 100049China

In order to meet the requirements for making organic solar cells(OSCs)through solution printing techniques,great efforts have been devoted into developing high performance OSCs with relatively thicker active *** this work,a thick-film(3.0 nm)ternary OSC with a power conversion efficiency of 14.3.is fabricated by introducing phenyl-C61-butyric-acid-methyl ester(PC61BM)into a PBDB-T-2Cl:BTP-4 F host *** addition of PC61BM is found to be helpful for improving the hole and electron mobilities,and thus facilitates charge transport as well as suppresses charge recombination in the active layers,leading to the improved efficiencies of OSCs with relatively thicker active *** results demonstrate the feasibility of employing fullerene derivative PC61BM to construct a high-efficiency thick-film ternary device,which would promote the development of thick layer ternary OSCs to fulfill the requirements of future roll to roll production.

关键词： ternary organic solar cells thick active layers carrier mobilities high efficiency

Embedding pyridine units in acceptors to construct donor-acceptor conjugated polymers

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chinese Chemical Letters 2019年第1期30卷 25-30页

作者： Zi-Yuan Wang Jie-Yu Wang jian Pei beijing National laboratory for molecular {3. Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education Key Laboratory of Polymer Chemistry and Physics of Ministry of Education Center for Soft Matter Science and Engineering College of Chemistry and Molecular Engineering Peking University

The development of donor-acceptor(D-A) conjugated polymers greatly promotes the device performance in organic electronics. Recently, the strategy of embedding pyridine units into D-A conjugated polymer backbones has attracted much attention due to the resulted lowered LUMO levels. In addition, the possible non-bonding interactions resulted from the nitrogen atoms also improve the coplanarity of the polymer backbones. All these factors have great contribution to enhance the device performance. In this review, we summarized the recent development of pyridine-embedded D-A conjugated polymers and their applications in organic field-effect transistors(OFETs).

关键词： Donor-acceptor Conjugated polymers Coplanarity Pyridine-embedded Organic electronics

Tunable Perpendicular Magnetic Anisotropy in Off-Stoichiometric Full-Heusler Alloy Co_2MnAl

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chinese physics Letters 2019年第6期36卷 79-82页

作者： Zhi-Feng Yu Jun Lu Hai-Long Wang xu-Peng Zhao Da-Hai Wei Jia-Lin Ma Si-Wei Mao jian-Hua Zhao state key laboratory of Superlattices and Microstructures Institute of SemiconductorsChinese Academy of Sciences center of Materials science and Optoelectronics Engineering University of Chinese Academy of Sciences beijing academy of Quantum Information sciences

Off-stoichiometric full-Heusler alloy Co_2 MnAl thin films with different thicknesses are epitaxially grown on GaAs(001) substrates by molecular-beam epitaxy. The composition of the films, close to Co_(1.65)Mn_(1.3.)Al(CMA),is determined by x-ray photoelectron spectroscopy and energy dispersive spectroscopy. Tunable perpendicular magnetic anisotropy(PMA) from 3.41 Merg/cm^3.to 1.88 Merg/cm^3.with the thickness increasing from 10 nm to 3. nm is found,attributed to the relaxation of residual compressive strain. Moreover, comparing with the ultrathin CoFeB/MgO used in the conventional perpendicular magnetic tunnel junction, the CMA electrode has a higher magnetic thermal stability with more volume involved. The PMA in CMA films is sustainable up to 3.0℃,compatible with semiconductor techniques. This work provides a possibility for the development of perpendicular magnetized full-Heusler compounds with high thermal stability and spin polarization.

关键词： Tunable Perpendicular Magnetic Anisotropy Full-Heusler Alloy Co2MnAl different thicknesses

An Asymmetric Non-fullerene Acceptor with Low Energy Loss and High Photovoltaic Efficiency

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chinese Journal of chemistry 2023年第9期41卷 1045-1050页

作者： Ye xu Jingwen Wang Huifeng Yao Pengqing Bi Tao Zhang jianmei xu jianhui Hou beijing National laboratory for molecular {3. State Key Laboratory of Polymer Physics and ChemistryCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China School of Chemical sciences University of Chinese Academy of SciencesBeijing 100049China state key laboratory of Photovoltaic {3. and Technology Trina SolarChangzhouJiangsu 213031China

Minimizing energy loss(E_(loss))plays a key role in improving the power conversion efficiencies(PCEs)of organic solar cells(OSCs).Here,to reveal the feasibility of the asymmetric molecular strategy in designing high-efficiency and low E_(loss)s OSC materials,we adopt the alkyl-alkoxy modification to design an asymmetric non-fullerene acceptor(NFA)named OC8-4F,where its symmetric alkyl-and alkoxy-substituted counterparties(2OC8-4F,eC9-4F)are also *** results suggest that the introduction of a symmetric alkoxy at the edge of eC9-4F can effectively decrease the lowest unoccupied molecular orbit level without greatly changing the highest occupied molecular orbit level,leading to a mediated *** the devices,the OC8-4F possesses well-balanced charge generation and E_(loss),giving the highest PCE of 18%.Our results imply that finely tuning the asymmetric structure can be used as an effective molecular design strategy to improve the photovoltaic performance of OSCs.

关键词： Organic photovoltaic cells Non-fullerene acceptors Asymmetric synthesis Energy conversion polymers