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Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells

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World Journal of Gastroenterology 2011年第36期17卷 4090-4098页

作者： Wen-Song Ge jian-xin wu jian-Gao Fan Ying-Wei Chen Yao-Jun Wang Department of Gastroenterology Shanghai Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Gastroenterology General Hospital of Jinan Military RegionJinan 250031Shandong ProvinceChina

AIM:To explore the role of high-mobility group box 1 (HMGB1) protein during liver fibrogenesis and investigate the functional effects of HMGB1 gene silencing in hepatic stellate cells (HSCs) using ***:Hepatic fibrosis in rats was induced through serial subcutaneous injections of dimethylnitrosamine,and expression of HMGB1 was detected by ***1 siRNAs were developed and transiently transfected into HSC-T6 cells using Lipofectamine ***1 expression was evaluated by real-time polymerase chain reaction (PCR) and Western blotting *** of α-smooth muscle actin (α-SMA) and collagen typesⅠand Ⅲ was evaluated by real-time *** proliferation and the cell cycle were determined using the methyl thiazolyl tetrazolium ***,collagen content in HSC supernatant was evaluated by an enzyme-linked immunosorbent ***:The results showed that HMGB1 was upregulated during liver fibrosis and that its expression was closely correlated with the deposition of *** molecules were successfully transfected into HSCs and induced inhibition of HMGB1 expression in a time-dependent ***,HMGB1 siRNA treatment inhibited synthesis of α-SMA and collagen types Ⅰ and Ⅲ in transfected ***:This study suggests a significant functional role for HMGB1 in the development of liver *** also demonstrates that downregulation of HMGB1 expression might be a potential strategy to treat liver fibrosis.

关键词： Hepatic fibrosis High-mobility group box 1 Hepatic stellate cells RNA interference

Detection of metastatic cancer cells in mesentery of colorectal cancer patients

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World Journal of Gastroenterology 2017年第34期23卷 6315-6320页

作者： Xue-Lai Luo Da-xing Xie jian-xin wu An-Ding wu Zong-Qing Ge Hai-Jie Li Jun-Bo Hu Zhi-xin Cao jian-Ping Gong Tongji Cancer Research Institute Department of Gastrointestinal SurgeryTongji HospitalTongji Medical College of Huazhong University of Science and Technology

AIM To detect the existence of isolated cancer cells in the mesentery of colorectum(named as Metastasis V), and investigate its clinical significance in colorectal cancer(CRC) *** Sixty-three CRC patients who received radical excision between January 2012 and September 2015 were included. All the patients underwent laparoscopyassisted radical colorectomy or proctectomy [with complete mesocolic excision(CME) or total mesorectal excision(TME)] with R0 dissections at the Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. The location and size of the primary lesions were recorded immediately after the tumor was removed, with the surrounding mesenterium completely separated along the intestinal wall. Each dissected mesentery sample was analyzed for hematoxylin-eosin staining and immunohistochemistry using cytokeratin 19 antibody. Image Pro Plus Software 6.0(Media Cybernetics, CA, United States) was usedto semi-quantitatively measure the concentration of the cytokeratin 19 immunohistochemistry. The correlation between metastasis found in mesentery and clinicopathological characteristics was examined. The prognosis of patients was also evaluated by preoperative serum CEA *** Metastasis V was detected in 14 of 63(22.2%) CRC patients who underwent laparoscopy-assisted radical colorectomy or proctectomy(with CME or TME) with R0 dissection in our hospital between January 2012 and September 2015. There was no significant difference in age, gender, tumor size, and tumor location in patients with Metastasis V(P > 0.05). Metastasis V was more likely to occur in poorly differentiated tumor(5/11; 45.5%) than moderately(8/46; 17.4%) and welldifferentiated one(1/6; 16.7%). The Metastasis V in N2 stage(9/14; 64.3%) was more frequent that in the N0 stage(3/35; 8.6%) or N1 stages(2/14; 14.3%). In addition, Metastasis V was positively related to the tumor invasive depth(T1:0/1, 0%; T2:1/12,

关键词： Colorectal cancer Colorectal mesentery Serum CEA level Radical surgery Metastasis V

Unusual Patterns of Neural Tube Defects in a High Risk Region of Northern China

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Biomedical and Environmental Sciences 2009年第4期22卷 340-344页

作者： GONG CHEN LI-JUN PEI jian HUANG xin-MING SONG LIANG-MING LIN XUE GU jian-xin wu FANG WANG JI-LEI wu JIA-PENG CHEN JU-FEN LIU RUO-LEI xin TING ZHANG XIAO-YING ZHENG Institute of Population Research Peking University Beijing 100871 China Institute of Nutrition and Food Safety Chinese Center for Disease Control and Prevention Beijing 100050 China Capital Institute of Pediatrics Beijing 100020 China

Objective To study the prevalence of different types of neural tube defects （NTDs） in Luliang Prefecture, Shanxi province, where the prevalence of NTDs is unusually high and the correlation between NTDs prevalence and patterns. Methods A surveillance population-based birth defects was performed in Luliang Prefecture, Shanxi province. Results The results of our study showed that the prevalence of NTDs was 2-fold higher in Luliang Prefecture than in other areas of Shanxi province. Unusual patterns of NTDs were found, however, multiple NTDs were relatively common in Luliang Prefecture, accounting for over 13% of all NTDs cases in China. Conclusion The prevalence of NTDs is associated with its patterns.

关键词： Neural tube defects Epidemiology Geographic variation

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Random Subspace Sampling for Classification with Missing Data

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Journal of Computer Science & Technology 2024年第2期39卷 472-486页

作者：曹云浩吴建鑫 State Key Laboratory for Novel Software Technology Nanjing UniversityNanjing 210093China

Many real-world datasets suffer from the unavoidable issue of missing values,and therefore classification with missing data has to be carefully handled since inadequate treatment of missing values will cause large *** this paper,we propose a random subspace sampling method,RSS,by sampling missing items from the corresponding feature histogram distributions in random subspaces,which is effective and efficient at different levels of missing *** most established approaches,RSS does not train on fixed imputed ***,we design a dynamic training strategy where the filled values change dynamically by resampling during ***,thanks to the sampling strategy,we design an ensemble testing strategy where we combine the results of multiple runs of a single model,which is more efficient and resource-saving than previous ensemble ***,we combine these two strategies with the random subspace method,which makes our estimations more robust and *** effectiveness of the proposed RSS method is well validated by experimental studies.

关键词： missing data random subspace neural network ensemble learning

Expression and alterations of different molecular form γ-glutamyl transferase and total RNA concentration during the carcinogenesis of rat hepatoma

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World Journal of Gastroenterology 1999年第4期5卷 84-86页

作者： TANG Qi Yun, YAO Deng Fu, LU jian xin, wu xin Hua and MENG Xian Yong

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关键词： liver neoplasms/enzymology γ glutamyl transferase/analysis disease models, animal RNA 2 fluoenyacetamide/analysis

Breaking the structural symmetry of active sites in zeolite imidazolate framework for enhanced CO2 photoreduction

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Science China Chemistry 2025年

作者： Yupeng Han Yayu Yan Qiaohong Li xin wu Fei Wang jian Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences University of Chinese Academy of Sciences College of Chemistry Fuzhou University

Adjusting the structure and composition of active sites is a highly effective method for activating linear ***, precise control of interfacial chemistry within molecular-scale environments presents a significant challenge in heterogeneous catalysis. In our study, we present symmetry-broken Mo-O-Co triple-atom active sites within the hybrid zeolite imidazole framework HZIF-CoMo. These sites disrupt the electronic balance and create a weak built-in electric field, thus promoting photocatalytic CO2reduction. The CO yield over HZIF-CoMo is improved to 3.1 mmol g-1h-1, which is over four times higher than the parent cobalt-imidazole framework MAF-6-Co. Both experimental and theoretical studies indicate that electronic modulation through the symmetry-breaking Mo-O-Co moiety acts as an organizing knob to accelerate charge separation and result in more carriers participating in the catalytic reaction. This work provides important insights into optimizing site-related electronic structures for targeted photocatalytic reactions.

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Estimation of Chloride Diffusivity in Hydrated Tricalcium Silicate Using a Hydration-Diffusion Integrated Method

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Journal of wuhan University of Technology(Materials Science) 2025年第1期40卷 49-64页

作者： WANG xin SHEN Dejian TAO Sijie LIU Ruixin wu Shengxing College of Civil and Transportation Engineering Hohai UniversityNanjing 210098China

This study aims to develop a chloride diffusion simulation method that considers the hydration microstructure and pore solution properties during the hydration of tricalcium silicate(C3S).The method combines the hydration simulation,thermodynamic calculation,and finite element analysis to examine the effects of pore solution,including effect of electrochemical potential,effect of chemical activity,and effect of mechanical interactions between ions,on the chloride effective diffusion coefficient of hydrated C3S *** results indicate that the effect of electrochemical potential on chloride diffusion becomes stronger with increasing hydration age due to the increase in the content of hydrated calcium silicate;as the hydration age increases,the effect of chemical activity on chloride diffusion weakens when the number of diffusible elements decreases;the effect of mechanical interactions between ions on chloride diffusion decreases with the increase of hydration age.

关键词： tricalcium silicate simulation method chloride diffusion coefficient pore solution

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High-throughput screening of CO_(2) cycloaddition MOF catalyst with an explainable machine learning model

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Green Energy & Environment 2025年第1期10卷 132-138页

作者： Xuefeng Bai Yi Li Yabo Xie Qiancheng Chen xin Zhang jian-Rong Li Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemical Engineering College of Materials Science&EngineeringBeijing University of TechnologyBeijing100124China

The high porosity and tunable chemical functionality of metal-organic frameworks(MOFs)make it a promising catalyst design ***-throughput screening of catalytic performance is feasible since the large MOF structure database is *** this study,we report a machine learning model for high-throughput screening of MOF catalysts for the CO_(2) cycloaddition *** descriptors for model training were judiciously chosen according to the reaction mechanism,which leads to high accuracy up to 97%for the 75%quantile of the training set as the classification *** feature contribution was further evaluated with SHAP and PDP analysis to provide a certain physical understanding.12,415 hypothetical MOF structures and 100 reported MOFs were evaluated under 100℃ and 1 bar within one day using the model,and 239 potentially efficient catalysts were *** them,MOF-76(Y)achieved the top performance experimentally among reported MOFs,in good agreement with the prediction.

关键词： Metal-organic frameworks High-throughput screening Machine learning Explainable model CO_(2)cycloaddition

Association between Long Interspersed Nuclear Element-1 Methylation and Relative Telomere Length in Wilms Tumor

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Chinese Medical Journal 2015年第22期128卷 3055-3061页

作者： Hui-Bo Chang Ji-Zhen Zou Cai He Rui Zeng Yuan-Yuan Li Fei-Fei Ma Zhuo Liu Hui Ye jian-xin wu Department of Biochemistry Capital institute of Pediatrics Beijing 100020 China Department of Pathology Capital institute of Pediatrics Beijing 100020 China Department of Surgery Capital institute of Pediatrics Beijing 100020 China

Background： DNA hypomethylation of long interspersed nuclear elements- 1 （LINEs- 1 ） occurs during carcinogenesis, whereas intbmaation addressing LINE-1 methylation in Wilms tumor （WT） is limited. The main purpose of our study was to quantity, LINE-1 methylation levels and evaluate their relationship with relative telomere length （TL） in WT. Methods： We investigated LINE-1 methylation and relative TL using bisulfite-polymerase chain reaction （PCR） pyrosequencing and quantitative PCR, respectively, in 20 WT tissues, 10 normal kidney tissues and a WT cell line. Significant changes were analyzed by t-tests. Results： LINE-1 methylation levels were significantly lower （P 〈 0.05） and relative TLs were sigmificantly shorter （P 〈 0.05） in WT compared with normal kidney. There was a significant positive relationship between LINE- 1 methylation and relative TL in WT （r = 0.671, P = 0.001 ）. LINE- 1 Methylation levels were significantly associated with global DNA methylation （r = 0.332, P 〈 0.01 ）. In addition, relative TL was shortened and LINE- 1 methylation was decreased in a WT cell line treated with the hypomethylating agent 5-aza-2＇-deoxycytidine compared with untreated WT cell line. Conclusion： These results suggest that LINE-1 hypomethylation is common and may be linked to telomere shortening in WT.

关键词： 5-aza-2'-deoxycytidine Hypomethylation Long Interspersed Nuclear Element-1 Relative Telomere Length Wilms Tumor

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