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Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

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World Journal of Stem Cells 2024年第3期16卷 305-323页

作者： jian-Jing Lin tong Ning Shi-Cheng Jia Ke-Jia Li Yong-Can Huang Qiang Liu jian-Hao Lin Xin-Tao Zhang Department of Sports Medicine and Rehabilitation Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Medical Science The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250033Shandong ProvinceChina Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijing 100871China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine ***,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple ***,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs *** To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with *** The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome *** were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA *** transcript was normalized by fragments per kilobase per *** change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction *** In total,263 DEGs were discovered,with 92 upregulated and 171 *** were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound *** cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and *** aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid *** real-time polymerase chain reaction confirmed targeted transcript *** Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 26

关键词： Nanosecond pulsed electric fields Whole transcriptome sequencing Mesenchymal stem cells Genetic response Stem cell engineering

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沥青混合料剪胀力学行为研究综述

长安大学学报（自然科学版） 2015年第1期35卷 33-40页

作者：张建同杨军东南大学交通学院江苏南京210096

为了分析沥青混合料剪胀力学行为,阐述了沥青混合料剪胀行为,回顾了剪胀相关试验及剪胀模型的现状和问题,并对其研究方向进行了展望。研究结果表明:沥青混合料剪胀行为在车辙产生中起着关键作用;采用X-ray CT技术、离散元方法等对颗粒... 详细信息

为了分析沥青混合料剪胀力学行为,阐述了沥青混合料剪胀行为,回顾了剪胀相关试验及剪胀模型的现状和问题,并对其研究方向进行了展望。研究结果表明:沥青混合料剪胀行为在车辙产生中起着关键作用;采用X-ray CT技术、离散元方法等对颗粒空间分布、大小、接触方式、破碎等进行定性及定量地分析沥青混合料变形机理和力学特性;通过建立微观力学模型,联系宏观力学变量与微观力学变量,使得微观测试、力学试验和数值模拟连为一体,预测沥青混合料剪胀行为,可为抗车辙沥青路面设计提供指导,开拓沥青混合料设计新思路。

关键词：道路工程沥青混合料剪胀行为微观力学综述

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扫描式数字X射线胸部摄影系统的研制

核电子学与探测技术 2006年第6期26卷 929-931页

作者：邢桂来童建民清华大学核能与新能源技术研究院北京100084

扫描式数字X射线摄影系统属DR范畴,除具有DR的一般优点外,还具有散射射线影响小(零本底)、低剂量和低成本三大优点。探讨了以固体线阵探测器为基础的扫描式数字X射线胸部摄影系统的构建,包括系统整体设计方案、关键技术问题的阐述以及... 详细信息

扫描式数字X射线摄影系统属DR范畴,除具有DR的一般优点外,还具有散射射线影响小(零本底)、低剂量和低成本三大优点。探讨了以固体线阵探测器为基础的扫描式数字X射线胸部摄影系统的构建,包括系统整体设计方案、关键技术问题的阐述以及系统性能分析,并提出了进一步提高系统性能的可行方法。

关键词：扫描式 X射线胸部摄影 DR

Characteristics and risk factor analyses of high-grade intraepithelial neoplasia in older patients with colorectal polyps

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World Journal of Gastrointestinal Oncology 2024年第10期16卷 4129-4137页

作者： Xin Zhang Ying Wang tong Zhu jian Ge Jun-Hua Yuan Department of Geriatric Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND According to the degree of intradermal neoplasia in the colorectal exhalation,it can be divided into two grades:Low-grade intraepithelial neoplasia(LGIN)and high-grade intraepithelial neoplasia(HGIN).Currently,it is difficult to accurately diagnose LGIN and HGIN through imaging,and clinical diagnosis depends on postoperative histopathological diagnosis.A more accurate method for evaluating HGIN preoperatively is urgently needed in the surgical treatment and nursing intervention of colorectal *** To explore the characteristics and risk factors of HGIN in older patients with colorectal *** We selected 84 older patients diagnosed with HGIN as the HGIN group(n=95 colonic polyps)and 112 older patients diagnosed with LGIN as the LGIN group(n=132 colonic polyps)from Shandong Provincial Hospital Affiliated to Shandong First Medical *** endoscopic features,demographic characteristics,and clinical manifestations of the two patient groups were compared,and a logistic regression model was used to analyze the risk factors for HGIN in these *** The HGIN group was older and had a higher number of sigmoid colon polyps,rectal polyps,pedunculated polyps,polyps≥1.0 cm in size,polyps with surface congestion,polyps with surface depression,and polyps with villous/tubular adenomas,a higher proportion of patients with diabetes and a family history of colorectal cancer,patients who experienced rectal bleeding or occult blood,patients with elevated carcinoembryonic antigen(CEA)and cancer antigen 199(CA199),and lower nutritional levels and higher frailty *** polyp location(in the sigmoid colon or rectum),polyp diameter(≥1.0 cm),pathological diagnosis of(villous/tubular adenoma),family history of colorectal cancer,rectal bleeding or occult blood,elevated serum CEA and CA199 levels,lower nutritional levels and higher frailty levels also are independent risk factors for *** The occurrence of high-grade neoplastic transformat

关键词： Elderly Colorectal polyps High-grade intraepithelial neoplasia Low-grade intraepithelial neoplasia Risk factors

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骨髓基质干细胞分化为神经细胞的研究进展

中国药理学通报 2006年第7期22卷 792-796页

作者：高博童建苏州大学医学院药理学教研室江苏苏州215123 苏州大学放射医学与公共卫生学院江苏苏州215123

骨髓基质干细胞具有广泛的分化能力,近年来发现其可以分化为神经元样细胞,且经不同因素诱导,可以分化为特殊的神经元表型,如多巴胺能神经元、5-HT敏感神经元等。其分化机制尚未明确,可能是通过独立的调节机制控制,且受神经特异性基因的... 详细信息

骨髓基质干细胞具有广泛的分化能力,近年来发现其可以分化为神经元样细胞,且经不同因素诱导,可以分化为特殊的神经元表型,如多巴胺能神经元、5-HT敏感神经元等。其分化机制尚未明确,可能是通过独立的调节机制控制,且受神经特异性基因的表达数量调控,其中Sox、Pax、Notch等基因在其分化中起重要作用。尤其是RB/PBO基因更为重要,而RB基因则触发胆碱能神经分化。外源性因素如cAMP浓度等也有一定的影响。骨髓基质干细胞对神经退行性疾病的治疗作用已经得到证实,因其具有取材容易,分离、培养、扩增方法成熟,且不存在伦理学和免疫排斥问题,临床应用前景广阔。

关键词：骨髓基质干细胞神经元神经退行性疾病移植

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宋夏沿边蕃部封建生产关系的发展

宁夏社会科学 2007年第1期 116-120页

作者：佟建荣宁夏大学西夏学研究中心宁夏银川750021

宋夏西北边缘分布着大量的蕃部族帐。北宋中后期,在汉地农业文明的影响下,这些蕃部地区的封建生产关系有了一定程度的发展。本文主要讨论了其封建土地占有制及租佃关系的形成与发展。

关键词：蕃部封建生产关系封建土地占有制租佃

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河南栾川锅沟萤石矿地质特征及成因初探

中国非金属矿工业导刊 2024年第1期 47-50页

作者：沈瑞峰胡亚净王振强王建统李沐霏豆贯铭河南省第一地质矿产调查院有限公司河南洛阳471000 河南省金银多金属成矿系列与深部预测重点实验室河南洛阳471000

栾川县锅沟萤石矿是合峪地区新发现的萤石矿床,发育2条规模较大的萤石矿带,新发现萤石矿体3个,矿体受断裂构造的严格控制,长600~1200m。总结以往区域上萤石矿床成矿物质来源、成矿时代、包裹体及其同位素等方面的研究,表明区域上萤石矿... 详细信息

栾川县锅沟萤石矿是合峪地区新发现的萤石矿床,发育2条规模较大的萤石矿带,新发现萤石矿体3个,矿体受断裂构造的严格控制,长600~1200m。总结以往区域上萤石矿床成矿物质来源、成矿时代、包裹体及其同位素等方面的研究,表明区域上萤石矿床形成于118~126Ma,属早白垩世;成矿流体为低温度、低盐度、低密度流体,可能主要源于大气降水;赋矿围岩合峪岩体和太山庙岩体可能是萤石矿重要的物质来源。通过矿区地质特征和矿体特征与区域萤石矿床特征对比,发现锅沟萤石矿与区域上萤石矿床具有相似的成矿作用,初步认为锅沟萤石矿床成因类型为断裂控矿、中低温热液裂隙充填脉状矿床,深部有较大的找矿前景。

关键词：锅沟萤石矿地质特征成因分析栾川县

论马克思哲学真理观的三个特征——读《关于费尔巴哈的提纲》第2条

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烟台大学学报（哲学社会科学版） 2017年第5期30卷 12-17页

作者：童建安山东大学哲学与社会发展学院山东济南250100

在马克思的语境中,"真理"实质上是指共产主义的实现,即人的存在与本质、自由与必然等一系列二元对立的最终消解,从而达到人的异化的消除、人的本质的实现这样一个宏伟的历史目标。马克思哲学的真理观具有实践性、现实性和此岸性等三个... 详细信息

在马克思的语境中,"真理"实质上是指共产主义的实现,即人的存在与本质、自由与必然等一系列二元对立的最终消解,从而达到人的异化的消除、人的本质的实现这样一个宏伟的历史目标。马克思哲学的真理观具有实践性、现实性和此岸性等三个特征。没有实践性就不可能有真理的现实性和此岸性,实践性是其真理观的基石;离开了现实性和此岸性,实践性也就失去了存在的意义。因此,马克思哲学真理观的这三个特征之间,彼此是相辅相成的。

关键词：马克思真理观特征实践性现实性此岸性

Effect of fragile histidine triad gene transduction on proliferation and apoptosis of human hepatocellular carcinoma cells

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World Journal of Gastroenterology 2008年第23期14卷 3754-3758页

作者： Rong-Hua Xu Liang-Yan Zheng Dong-Lei He jian tong Li-Ping Zheng Wu-Ping Zheng Jin Meng Li-Ping Xia Cong-Jun Wang Ji-Lin Yi The Affiliated Hospital of Hainan Medical College Haikou 570102 Hainan Province China Department of General Surgery First People's Hospital Affiliated to Shanghai Jiaotong University Shanghai 200080 China Department of General surgery Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM:To evaluate the inhibitory effects of human fragile histidine triad(FHIT) gene on cell proliferation and apoptosis in human hepatocellular carcinoma line Hep3B in vitro. METHODS:A recombinant pcDNA3.1(+) /FHIT including the functional region of FHIT gene was constructed and transferred into human hepatocellular carcinoma cells in vitro. mRNA and protein expression of the FHIT gene in the transfected cells was detected by RT-PCR and Western blot,respectively. The effect of FHIT on proliferation was detected by MTT assay. Changes in cell cycle and apoptosis were assayed by flow cytometry. Five mice received subcutaneous transplantation of Hep3B-FHIT;5 mice received subcutaneous transplantation of normal Hep3B and Hep3B-C as controls. The body weight of nude mice and tumor growth were measured. RESULTS:RT-PCR and Western blot analysis showed that the expression level of FHIT-mRNA and FHIT protein was higher in Hep3B cells after infection withpcDNA3.1(+) /FHIT. The growth of Hep3B cells treated with pcDNA3.1(+) /FHIT was significantly inhibited. The pcDNA3.1(+) /FHIT-transfected Hep3B cells showed a significantly higher cell rate at G0-G1 phase and increased apoptosis in comparison with controls(P < 0.05) . The growth of transplanted tumor was inhibited markedly by FHIT. Tumors arising from the Hep3B-FHIT cells occurred much later than those arising from the Hep3B and Hep3B-C cells. The growth of Hep3B-FHIT cells was slow and the tumor volume was low. CONCLUSION:Transduction of FHIT gene inhibits the growth of human hepatocellular carcinoma cells and induces cell apoptosis in vivo and in vitro.

关键词： Hepatocellular carcinoma Gene therapy Fragile histidine triad gene