检索结果-南通市图书馆

Is 1, 25-dihydroxyvitamin D_3 an ideal substitute for dexamethasone for inducing osteogenic differentiation of human adipose tissue-derived stromal cells in vitro?

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2006年第15期119卷 1278-1286页

作者： ZHOU Yong-sheng LIU Yun-song TAN jian-guo Department of Prosthodontics School and Hospital of Stomatology Peking University Beijing 100081 China

Background Human adipose tissue-derived stromal cells （hADSCs） can be induced to differentiate along an osteoblastic lineage under stimulation of dexamethasone （DEX）. Recent studies, however, have questioned the efficacy of glucocorticoids such as DEX in mediating the osteogenesis process of skeletal progenitor cells and processed lipoaspirate cells. Is it possible to find a substitute for DEX？ Therefore, this study was designed to investigate osteogenic capacity and regulating mechanisms for osteoblastic differentiation of hADSCs by comparing osteogenic media （OM） containing either 1, 25-dihydroxyvitamin D3 （VD） or DEX and determine if VD was an ideal substitute for DEX as an induction agent for the osteogenesis of hADSCs. Methods Osteogenic differentiation of hADSCs was induced by osteogenic medium （OM） containing either 10 nmol/L VD or 100 nmol/L DEX. Differentiation of hADSCs into osteoblastic lineage was identified by alkaline phosphatase （ALP） staining, von Kossa staining, and reverse transcription-polymerase chain reaction assays for mRNA expression of osteogenesis-related genes such as type Ⅰ collagen （COL Ⅰ）, bone sialoprotein （BSP）, osteocalcin （OC）, bone morphogenetic protein （BMP）-2, BMP-4, BMP-6, BMP-7, runt-related transcription factor 2/core binding factor α1 （Runx2/Cbfal）, osterix （Osx）, and lim mineralization protein- 1 （LMP- 1）. Results von Kossa staining revealed that the differentiated cells induced by both VD and DEX were mineralized in vitro. They also expressed osteoblast-related markers, such as ALP, COL Ⅰ, BSP, and OC. Runx2/Cbfal, Osx, BMP-6, and LMP-1 were upregulated during VD and DEX-induced hADSC osteoblastic differentiation, but BMP-4, BMP-7 were not. BMP-2 was only expressed in VD-induced differentiated cells. Conclusions VD or DEX-induced hADSCs differentiate toward the osteoblastic lineage in vitro. Runx2/Cbfal, Osx, BMP-2, BMP-6, and LMP-1 are involved in regulating osteoblastic differentiation of hADSCs, but BMP-4, BMP-7 are not.

关键词： human adipose tissue-derived stromal cell osteoblast 1, 25-dihydroxyvitamin D3 dexamethasone

生长抑制因子4对人黑素瘤细胞株M14凋亡的调控

在线全文

学校读者我要写书评

暂无评论

中国皮肤性病学杂志 2008年第10期22卷 577-580页

作者：蔡丽敏李海燕孙建方中国医学科学院皮肤病研究所中国协和医科大学皮肤病医院病理科江苏南京210042 黑龙江省肿瘤医院皮肤科黑龙江哈尔滨150076

目的探讨生长抑制因子4对人黑素瘤细胞株M14凋亡的调控及其作用机制。方法构建人生长抑制因子4基因的真核表达质粒,并将其转导入M14细胞,采用流式细胞术及TUNEL法检测细胞的凋亡情况,同时采用蛋白印迹法分析凋亡通路的活化及通路相关蛋... 详细信息

目的探讨生长抑制因子4对人黑素瘤细胞株M14凋亡的调控及其作用机制。方法构建人生长抑制因子4基因的真核表达质粒,并将其转导入M14细胞,采用流式细胞术及TUNEL法检测细胞的凋亡情况,同时采用蛋白印迹法分析凋亡通路的活化及通路相关蛋白的表达情况。结果转染外源生长抑制因子4基因后,M14细胞出现了明显的凋亡峰并伴有G2/M期阻滞,凋亡率及凋亡指数也明显高于转染空载质粒及未转染M14细胞,差异有显著性意义(P<0.01)。还发现转染外源生长抑制因子4基因后,M14细胞凋亡通路活化,抗凋亡蛋白Bcl-2表达下调,而促凋亡蛋白Bax表达上调,凋亡执行蛋白Cyt-c表达增加而caspase3表达下降。结论生长抑制因子4可能通过活化凋亡通路,进而促进M14细胞凋亡。这可能也是ING4抑制M14细胞增殖的机制之一。

关键词：生长抑制因子4 黑素瘤凋亡

A novel method for extracting skeleton of fruit treefrom 3D point clouds

在线全文

学校读者我要写书评

暂无评论

International Journal of Modeling, Simulation, and Scientific Computing 2020年第6期11卷 78-89页

作者： Shenglian Lu Guo Li jian Wang College of Computer Science and Information Technology Guangxi Normal University Guilin 541004P.R.China Guangxi Key Lab of Multi-Source Information Mining and Security No.15 Yucai Road Guilin 541004P.R.China Center of Information Technology Beijing Municipal Bureau of Agriculture and Rural Affairs No.6 Yumin Middle Rood Beijing 100029P.R.China

Tree skeleton could be useful to agronomy researchers because the skeleton describes the shape and topological structure of a *** phenomenon of organs’mutual occlusion in fruit tree canopy is usually very serious,this should result in a large amount of data missing in directed laser scanning 3D point clouds from a fruit ***,traditional approaches can be ineffective and problematic in extracting the tree skeleton correctly when the tree point clouds contain occlusions and missing *** overcome this limitation,we present a method for accurate and fast extracting the skeleton of fruit tree from laser scanner measured 3D point *** proposed method selects the start point and endpoint of a branch from the point clouds by user’s manual interaction,then a backward searching is used to find a path from the 3D point cloud with a radius parameter as a *** experimental results in several kinds of fruit trees demonstrate that our method can extract the skeleton of a leafy fruit tree with highly accuracy.

关键词： Skeleton extraction fruit tree 3D point cloud modeling plant structure

基于ACSEEMD和模糊C均值聚类的风电机组齿轮箱故障预警

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

电子器件 2019年第6期42卷 1410-1415页

作者：张健江苏安全技术职业学院电气工程系

针对风电机组齿轮箱中齿轮故障诊断问题,提出一种风电齿轮箱故障预警方法。该方法将自适应完全集合经验模态分解ACSEEMD(Adaptive Complete Set of Ensemble Empirical Mode Decomposition)、模糊C均值聚类FCMC(Fuzzy C Mean Clustering... 详细信息

针对风电机组齿轮箱中齿轮故障诊断问题,提出一种风电齿轮箱故障预警方法。该方法将自适应完全集合经验模态分解ACSEEMD(Adaptive Complete Set of Ensemble Empirical Mode Decomposition)、模糊C均值聚类FCMC(Fuzzy C Mean Clustering)算法和Hotelling T 2统计量相结合,通过对风电齿轮箱振动信号进行ACSEEMD-FCMC降噪处理,再利用FCMC对降噪后的多组振动信号进行融合分析,提取出连续的T 2值(C-T 2)及其均方根值RMS(C-T 2),设定报警阈值RMS(C-T 2)lim,最后,建立风电齿轮箱故障预警模型。实验结果表明:该方法对风电齿轮箱振动信号降噪效果显著,可有效解决多组振动信号报警结果不一致的问题。

关键词：自适应完全集合经验模态分解模糊C均值聚类风电齿轮箱故障预警

石墨/[Li(Ni_(0.4)Co_(0.2)Mn_(0.4))O_2+limn_2O_4]电池循环过程的研究

在线全文

学校读者我要写书评

暂无评论

电源技术 2013年第2期37卷 183-186页

作者：张建刘浩涵杨传铮夏保佳中国科学院上海微系统与信息技术研究所上海200050

研究了石墨/[Li(Ni0.4Co0.2Mn0.4)O2+limn2O4]锂离子电池在循环过程中容量的衰减和正负极活性材料精细结构的变化。Li(Ni0.4Co0.2Mn0.4)O2在循环过程中晶体结构的保持能力要优于limn2O4。循环过程中正负极活性材料点阵参数、微晶尺寸、... 详细信息

研究了石墨/[Li(Ni0.4Co0.2Mn0.4)O2+limn2O4]锂离子电池在循环过程中容量的衰减和正负极活性材料精细结构的变化。Li(Ni0.4Co0.2Mn0.4)O2在循环过程中晶体结构的保持能力要优于limn2O4。循环过程中正负极活性材料点阵参数、微晶尺寸、堆垛无序和微应变等精细结构的演变与电池的循环性能具有关联性。

关键词：锂离子电池精细结构循环性能

中国山东与新加坡华人内皮一氧化氮基因多态的比较及与糖尿病肾病的关系

在线全文

学校读者我要写书评

暂无评论

中华医学杂志 2007年第48期87卷 3415-3417页

作者：董建军赵家军李国安林敏箐陈淑薇廖琳山东大学齐鲁医院内分泌科济南250012 山东省立医院内分泌科新加坡国立大学医学院内分泌实验室

目的探讨内皮一氧化氮合酶(eNOS)基因内含子4的多态性在中国山东与新加坡华人糖尿病患者中的分布及与糖尿病肾病的关系。方法 237例2型糖尿病患者入选(山东华人87例、新加坡华人150例)。从全血中提取 DNA 进行多聚酶链反应、克隆及测序... 详细信息

目的探讨内皮一氧化氮合酶(eNOS)基因内含子4的多态性在中国山东与新加坡华人糖尿病患者中的分布及与糖尿病肾病的关系。方法 237例2型糖尿病患者入选(山东华人87例、新加坡华人150例)。从全血中提取 DNA 进行多聚酶链反应、克隆及测序。结果新加坡华人中该基因多态性有3种等位基因,发现了一种新的等位基因,命名为 c,有6个27 bp 重复序列;本土华人中只有2种等位基因。等位基因 a、b 和 c 均与糖尿病。肾病无显著相关性,但等位基因 a 在中国山东与新加坡人群中的分布差异存在统计学意义(P<0.01)。结论 eNOS 基因内含子4的多态性有3种等位基因;该基因多态性与糖尿病肾病无相关性。

关键词：一氧化氮合酶内皮性基因糖尿病肾病

Impact of LDB3 gene polymorphisms on clinical presentation and implantable cardioverter defibrillator(ICD) implantation in Chinese patients with idiopathic dilated cardiomyopathy

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第9期20卷 766-775页

作者： Dong-fei WANG Jia-lan LYU Juan FANG jian CHEN Wan-wan CHEN Jia-qi HUANG Shu-dong XIA jian-mei JIN Fang-hong DONG Hong-qiang CHENG Ying-ke XU Xiao-gang GUO Department of Cardiology the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China Department of Cardiology the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Department of Cardiology Pujiang Branch of the First Affiliated HospitalSchool of MedicineZhejiang UniversityJinhua 322200China Department of Pathology and Pathophysiology School of MedicineZhejiang UniversityHangzhou 310058China Department of Cardiology the Forth Affiliated HospitalSchool of MedicineZhejiang UniversityYiwu 322000China Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical EngineeringZhejiang UniversityHangzhou 310027China

Objective:Mutations in lim domain binding 3(LDB3)gene cause idiopathic dilated cardiomyopathy(IDCM),a structural heart disease with a complicated genetic ***,the association of polymorphisms in the LDB3 gene with susceptibility to IDCM in Chinese populations remains unexplored as dose the impact on clinical ***:We sequenced all exons and the adjacent part of introns of the LDB3 gene in 159 Chinese han IDCM patients and 247 healthy *** we detected the distribution of polymorphisms in the LDB3 gene in all participants and assessed their associations with risk of ***,we conducted a stratified genotype–phenotype correlation ***:The A allele of rs4468255 was significantly associated with IDCM(Phan population.A allele of rs4468255 is higher in IDCM patients with ICD implantation,suggesting the influence of genetic background in the generation of this *** addition,rs11812601,rs56165849,and rs3740346 in LDB3 show association with brain natriuretic peptide,DBP,and LVEF levels in patients with IDCM but did not show any association with IDCM susceptibility.

关键词： Idiopathic dilated cardiomyopathy Implantable cardioverter defibrillator(ICD) lim domain binding 3(LDB3) Polymorphism han Chinese

在线全文

学校读者我要写书评

暂无评论

三视场红外搜索光学系统的设计

应用光学 2013年第3期34卷 397-401页

作者：胡博杨子建韩昆烨高婧王凌张宣智西安应用光学研究所陕西西安710065

设计一款实际工程应用的红外三视场光学系统,其中大中视场利用透镜组切换变倍,小视场和大视场利用反射镜切换变倍。设计中采用二次成像的方式,3个视场共用二次成像透镜组,保证100%冷屏效率,减小第1片透镜的过口径。同时,采用非球面技术... 详细信息

设计一款实际工程应用的红外三视场光学系统,其中大中视场利用透镜组切换变倍,小视场和大视场利用反射镜切换变倍。设计中采用二次成像的方式,3个视场共用二次成像透镜组,保证100%冷屏效率,减小第1片透镜的过口径。同时,采用非球面技术校正系统的球差和彗差,通过光学设计软件CODE V仿真,得出最大的点列斑为11μm左右,并且MTF接近衍射极限,成像质量完全满足使用要求。最后,该系统利用反射镜折叠光路实现了系统结构紧凑、体积小的特点。

关键词：红外光学系统二次成像非球面光学设计

CHARACTERISTIC OF COMPOSITION MEROMORPHIC FUNCTIONS WITH DEFICIENT FUNCTIONS

在线全文

学校读者我要写书评

暂无评论

佳木斯大学学报（自然科学版） 2004年第4期22卷 536-539页

作者： SUN jian-wu (Department of Mathematics,Huaiyin Teachers College Huaiyin 223001,China.) 第4期SUNjianwu:CharacteristicofCompositionMeromorphicFunctionswithDeficientFunctions

In this paper, we obtain the following result: Let f(z) be a meromorphic function of order p and lower order λ，let ai(z)(i=1,2,…n，n≤∞）be entire function, which satisying T（r，ai(z)=o(T(r,f)) with ∑i=1^n δ（ai(z),f)=1 and δ（ai(z),f)>0(ai(z)≠∞）.If lim r→∞ T（r，f(z+1))/T(r,f(z))=∞ then p=λ=∞.We solved problem due to C. C. yang concerning the characteristic functions of the composite functions.

关键词：亚纯函数复合函数全纯函数极限