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Characteristics and Influencing Factors of the Microbial Concentration and Activity in Atmospheric Aerosols over the South China Sea

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Journal of Ocean University of China 2021年第2期20卷 257-270页

作者： QI Jianhua YIN Yidan XIE jiamin LI Mengzhe DIng Xue LI Hongtao Key Laboratory of Marine Environment and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266100China

Oceans are important sources of microbes in atmospheric aerosols;however, information about the characteristics of airborne microbes and their influencing factors over oceans is lacking. Here we report the characteristics of the microbial abundance and activity in aerosols sampled near the sea surface over the South China Sea(SCS) from May to June 2016. The airborne microbial concentration range in the aerosols was 1.68?105 to 4.84?105 cells m-3 over the SCS, reflecting an average decrease of 40% – 54% over the SCS compared with that in the samples from the coastal region of Qingdao. About 63% – 76% of the airborne microbes occurred in coarse particles(> 2.1 ?m), with a variable size distribution over the SCS. The microbial activity range in aerosols, measured by the fluorescein diacetate(FDA) hydrolysis method, was 2.09 – 11.97 ng m-3 h-1 sodium fluorescein(SF) over the SCS, which was 15% – 79% lower than that over the coastal region. These values reflected a different spatial distribution over the SCS from that of the microbial concentration. Except for certain samples, all samples had 68% of the microbial activity occurring in coarse particles. Correlation analysis showed that the microbial abundance and activity were positively correlated with the aerosol, organic carbon(OC), and water-soluble organic carbon(WSOC) concentrations, indicating that the airborne microbes may be related to the reactions of certain water-soluble organic chemicals in the atmosphere. Moreover, the concentrations of airborne microbes were significantly negatively correlated with the horizontal offshore distance. The microbial concentration and activity were significantly correlated with wind speed.

关键词： microbes aerosols microbial activity size distribution WSOC

Npac Is A Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 110-128页

作者： Sue Yu Jia Li Guanxu Ji zhen Long ng jiamin Siew Wan Ning Lo Ying Ye Yuan Yuan Chew Yun Chau Long Wensheng zh.ng Ernesto Guccione Yuin Han Loh zhi-Hong Jiang Henry Yang Qiang Wu Department of Biochemistry Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Cancer Science Institute of Singapore Centre for Translational MedicineSingapore 117599Singapore The State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and TechnologyMacao Special Administrative Region 999078China Cam-Su Genomic Resource Center Soochow UniversitySuzhou 215123China Institute of Molecular and Cell Biology Singapore 138673Singapore

Chromatin modification contributes to pluripotency maintenance in embryonic stem cells(ESCs).However,the related mechanisms remain ***,we show that Npac,a"reader"of histone H3 lysine 36 trimethylation(H3K36me3),is required to maintain mouse ESC(mESC)pluripotency since knockdown of Npac causes mESC *** of Npac in mouse embryonic fibroblasts(MEFs)inhibits reprogramming ***,our chromatin immunoprecipitation followed by sequencing(ChIP-seq)results of Npac reveal that Npac co-localizes with histone H3K36me3 in gene bodies of actively transcribed genes in ***,we find that Npac interacts with positive transcription elongation factor b(p-TEFb),Ser2-phosphorylated RNA PolⅡ(RNA PolⅡSer2P),and Ser5-phosphorylated RNA PolⅡ(RNA PolⅡSer5 P).Furthermore,depletion of Npac disrupts transcriptional elongation of the pluripotency genes Nanog and *** together,we propose that Npac is essential for the transcriptional elongation of pluripotency genes by recruiting p-TEFb and interacting with RNA PolⅡSer2P and Ser5P.

关键词： Npac Pluripotency Reprogramming Histone H3K36me3 Transcriptional elongation

经动脉化疗栓塞术单用及联合靶向药物治疗中期肝癌的肝功能变化及预后比较

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解放军医学院学报 2024年第3期45卷 230-238页

作者：王沫萱程家敏千年松解放军医学院北京100853 解放军总医院第五医学中心肝病医学部肝病科北京100039 解放军总医院第八医学中心呼吸与危重症医学部胸部肿瘤科北京100091

背景经动脉化疗栓塞术(transcatheter arterial chemoembolization,TACE)和酪氨酸激酶抑制剂(tyrosine kinase inhibitors,TKI)是原发性肝细胞癌(hepatocellular carcinoma,HCC)治疗的常用手段,然而关于TACE与TACE-TKI对中期HCC术后肝功能的影响及预后差异的临床研究仍缺乏。目的比较TACE与TACE-TKI(索拉非尼或仑伐替尼)治疗中期HCC的短期肝功能变化、并发症、生存情况[无进展生存期(progression free survival,PFS)]及其关联因素。方法收集2010年6月—2023年1月规律就诊于解放军总医院第五医学中心肝脏肿瘤科的中期HCC患者149例,依据治疗方法将其分为TACE组和TACE-TKI组,研究随访至2023年6月,对首次TACE治疗前、治疗后48 h、治疗后2个月的肝功能、术后并发症及生存预后进行分析。结果149例患者中,TACE组97例,TACE-TKI组52例(索拉非尼组27例,仑伐替尼组25例)。两组基线特征差异无统计学意义(P>0.05)。术后48 h TACE-TKI组肝功能不全占比高于TACE组(42.3%vs21.6%,P=0.008);白细胞、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总胆红素均较术前升高(Png/mL(HR=0.570,95%CI:0.357~0.909,P=0.018)、天冬氨酸氨基转移酶≤31μ/L(HR=0.527,95%CI:0.319~0.872,P=0.013)、凝血酶原活动度≤93%(HR=0.507,95%CI:0.310~0.832,P=0.007)、TACE次数>2次(HR=2.104,95%CI:1.359~3.259,P=0.001)、单用TACE治疗方案(HR=0.598,95%CI:0.380~0.942,P=0.027)患者的PFS更长。结论相比于TACE,TACE-TKI治疗中期肝细胞癌时,发生肝功能不全和术后并发症的概率更高。对于接受TACE或TACE-TKI治疗的中期肝细胞癌患者,甲胎蛋白>647 ng/mL、TACE次数≤2次、天冬氨酸氨基转移酶>31μ/L、凝血酶原活动度>93%、使用TACETKI方案在一定范围内与更差的PFS存在相关性。

关键词：中期肝细胞癌经动脉化疗栓塞术索拉非尼仑伐替尼肝功能术后并发症

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Characteristics and health risks of population exposure to phthalates via the use of face towels

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Journal of Environmental Sciences 2023年第8期130卷 1-13页

作者： Wenhui zh.ng Na zh.ng Sujing Wang Siyu Sun Qirui An Xiaoqian Li Zimeng Li Yining Ji Yunyang Li jiamin Pan College of New Energy and Environment Jilin UniversityChangchun 130012China Key Laboratory of Groundwater Resources and Environment Ministry of EducationJilin UniversityChangchun 130012China Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchun 130102China

The production of face towels is growing at an annual rate of about 4%in China,reaching 1.13 million tons by ***(PAEs)are widely used in textiles,and face towels,as an important household textile,may expose people to PAEs via the skin,further leading to health *** collected new face towels and analyzed the distribution characterization of PAEs in *** changes of PAEs were explored in a face towel use experiment and a simulated laundry *** on the use of face towels by 24 volunteers,we calculated the estimated daily intake(EDI)and comprehensively assessed the hazard quotient(HQ),hazard index(HI),and dermal cancer risk(DCR)of PAEs exposure in the *** were present in new face towels at total concentrations ofng/g,with a median of 173.2 ng/g,which was a lower contamination level compared with other *** contents in used face towels were significantly higher than in new face *** concentrations of PAEs in coral velvet were significantly higher than those in *** washing removed some PAEs,while detergent washing increased the PAE content on face ***,weight,use time,and material were the main factors affecting *** HQ and HIwere less than 1,which proved PAEs had no significant non-carcinogenic health *** the five target PAEs studied,DEHPwas the only carcinogenic PAE andmay cause potential health risks after long-term ***,we should pay more attention to DEHP.

关键词： Face towels Phthalates Dermal exposure Estimated daily intake Risk assessment

Editing of Rice Endosperm Plastidial Phosphorylase Gene OsPho1 Advances Its Function in Starch Synthesis

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Rice science 2021年第3期28卷 209-211页

作者： LIU Song SHAO Gaoneng JIAO Guiai zhU Maodi WU jiamin CAO Ruijie CHEN Yujuan XIE Lihong SHEng zh.nghua TAng Shaoqing China National Rice Research Institute Hangzhou 310006China

OsPho1 in zh.nghua 11(zh11)was edited using the clustered regularly interspaced short palindromic repeatsassociated endonuclease 9(CRISPR/Cas9)*** homozygous T1 mutants(cr-pho1-34 and cr-pho1-37)displayed a chalky endosperm with a white core,which significantly decreased 1000-grain *** addition,many rounded starch granules and abnormal amyloplasts were present in the central region of mutant endosperm cells with increased amylose and lipid contents,decreased total protein content,and altered physicochemical properties of *** OsPho1 protein is localized in chloroplasts,and quantitative real-time PCR(qRT-PCR)andβ-glucuronidase(GUS)staining indicated that OsPho1 was highly expressed in seeds at 5 d after fertilization(DAF).OsPho1 mutations displayed close relationships with plastidial phosphoglucomutase and ADPGlc pyrophosphorylase based onα-D-glucose-1P at different ***,the expressions of starch metabolismrelated genes were also altered in the mutant,and the overexpression of OsPho1 may cause grain chalkiness.

关键词： CRISPR/Cas9 Pho Starch

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增益平坦的全光波长转换技术研究

光通信技术 2018年第8期42卷 1-5页

作者：巩稼民侯玉洁蔡庆任帆丁哲西安邮电大学电子工程学院西安710121

为避免多路波长转换实现增益平坦时引起的码间串扰和非线性影响,利用喇曼增益效率系数在L波段近似为常数的非对称双芯光子晶体光纤(ATC-PCF),设计了一种增益平坦的全光波长转换方案。建立了基于ATC-PCF中受激喇曼散射(SRS)效应的多路波... 详细信息

为避免多路波长转换实现增益平坦时引起的码间串扰和非线性影响,利用喇曼增益效率系数在L波段近似为常数的非对称双芯光子晶体光纤(ATC-PCF),设计了一种增益平坦的全光波长转换方案。建立了基于ATC-PCF中受激喇曼散射(SRS)效应的多路波长转换理论模型,给出了多路波长转换器的原理框图,并通过Matlab进行仿真分析。结果表明:所设计的波长转换器平坦度为0.3359dB,带宽为60nm。当泵浦光功率为1.6W、光纤长度为1.5km时,平坦度都可以保持在1dB内,为设计增益平坦的多路波长转换器提供了新的理论基础。

关键词：波长转换受激喇曼散射效应非对称双芯光子晶体光纤增益平坦

急慢性乙型肝炎患者外周血调节性T细胞鉴定与临床意义

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临床医学工程 2016年第12期23卷 1639-1640页

作者：关阳东关瑞宁朱嘉敏周若章开平市中心医院广东开平529300

目的探讨急慢性乙型肝炎患者外周血调节性T细胞的鉴定与临床意义。方法选择本院收治的急慢性乙型肝炎患者82例,其中急性乙肝患者(AHB)40例,慢性乙肝患者(CHB)42例,同期入选体检健康者40例为对照组。应用流式细胞仪分析Treg细胞表面及细... 详细信息

目的探讨急慢性乙型肝炎患者外周血调节性T细胞的鉴定与临床意义。方法选择本院收治的急慢性乙型肝炎患者82例,其中急性乙肝患者(AHB)40例,慢性乙肝患者(CHB)42例,同期入选体检健康者40例为对照组。应用流式细胞仪分析Treg细胞表面及细胞内分子表达、Treg频率,应用实时荧光定量RT-PCR检测血清HBV DNA载量;评估三组外周血调节性T细胞表面、细胞内分子表达、Treg频率水平,评估急慢性乙型肝炎患者的血清HBV DNA载量指标。结果慢性乙型肝炎患者的Treg占CD4^+T细胞频率显著高于急性乙型肝炎组,差异有统计学意义(P0.05);急性乙型肝炎组与健康对照组比较差异有统计学意义(P<0.05)。慢性乙型肝炎组在高病毒载量患者的Treg频率为(4.6±1.8)%,显著高于低病毒载量患者的(3.2±0.7)%,差异有统计学意义(t=5.22,P<0.05);急性乙型肝炎患者恢复期的Treg频率为(6.1±1.6)%,显著高于急性期的(3.0±0.8)%,差异有统计学意义(t=10.25,P<0.05)。结论慢性乙型肝炎患者高病毒载量组的CD4^+Treg频率显著增高,说明Treg可通过抑制细胞免疫反应影响病毒清除;急性乙型肝炎早期患者的Treg较低,随后增高,可能与急性乙型肝炎不同发病阶段的Treg所起作用密切相关。

关键词：临床研究免疫耐受 T淋巴细胞急慢性乙型肝炎

急性心肌梗死患者血浆B型钠尿肽浓度与心肌梗死面积的相关性

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临床心血管病杂志 2010年第5期26卷 360-363页

作者：牛甲民秦爱丽莱芜市人民医院心内科山东莱芜271100

目的:研究急性心肌梗死(AMI)患者血浆B型钠尿肽(BNP)浓度与心肌梗死(MI)面积的相关性。方法:AMI患者80例为AMI组,30例健康志愿者为对照组,2组均行血浆BNP检测、心脏磁共振检查,分析BNP浓度与MI面积的关系。结果:AMI组血浆BNP与MI面积呈... 详细信息

目的:研究急性心肌梗死(AMI)患者血浆B型钠尿肽(BNP)浓度与心肌梗死(MI)面积的相关性。方法:AMI患者80例为AMI组,30例健康志愿者为对照组,2组均行血浆BNP检测、心脏磁共振检查,分析BNP浓度与MI面积的关系。结果:AMI组血浆BNP与MI面积呈正相关(r=0.645,Png/L时,诊断MI面积>10%的敏感性为85.1%,特异性为70.4%。结论:AMI患者血浆BNP浓度与MI面积密切相关,检测BNP浓度可以粗略评估MI面积。

关键词：心肌梗死 B型钠尿肽心肌梗死面积磁共振灌注成像

蛋白质组学非靶向性质谱分析结合化学计量学在牛肉掺假初筛中的应用

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食品与发酵工业 2023年第3期49卷 290-295页

作者：蒲科源丘嘉敏刘柏霖童永祺程子彬刘诚林艳吴坤明汕头大学化学系广东省有序结构材料的制备与应用重点实验室广东汕头515063 汕头大学生物系广东汕头515063 汕头大学计算机科学与技术系广东汕头515063 汕头大学医学院第二附属医院广东汕头515041

牛肉作为高价值食用肉类一直是肉制品造假的重灾区,构建一种高通量、快速、经济的牛肉鉴别方法势在必行。该研究在简单、快速地完成生、熟牛鸡鸭猪4种肉的蛋白质提取后,利用具有高通量、操作简便优势的基质辅助激光解析电离飞行时间质谱... 详细信息

牛肉作为高价值食用肉类一直是肉制品造假的重灾区,构建一种高通量、快速、经济的牛肉鉴别方法势在必行。该研究在简单、快速地完成生、熟牛鸡鸭猪4种肉的蛋白质提取后,利用具有高通量、操作简便优势的基质辅助激光解析电离飞行时间质谱,采集蛋白质质谱数据,共获得生肉的129个离子峰和熟肉的151个离子峰,经数据预处理后,采用随机森林算法筛选到11个能区分4种肉的特征蛋白质,再结合主成分分析数据降维与数据可视化的优势,构建牛肉掺假鉴别模型,最后通过完成对掺假牛肉样本的检测以验证模型。结果表明,4种肉均能通过该方法得到较好的区分,且生熟状态下的掺假牛肉均能得到鉴别,证明该方法能准确、简便地完成生熟牛肉掺假初筛的目标。

关键词：基质辅助激光解析电离飞行时间质谱肉类掺假机器学习特征蛋白质主成分分析