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Neuropeptide Y receptor Y8b(npy8br)regulates feeding and digestion in Japanese medaka(Oryzias latipes)larvae:evidence from gene knockout

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Journal of Zhejia.g University-Science B(Biomedicine & Biotechnology) 2024年第7期25卷 605-616页

作者： Xiaodan jia.Ke lu Xufang LIANG College of Fisheries Chinese Perch Research CenterHuazhong Agricultural UniversityWuhan 430070China Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt Ministry of EducationWuhan 430070China

Neuropeptide Y receptor Y8(NPY8R)is a fish-specific receptor with two subtypes,NPY8AR and *** in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation;this has been found in only a few fish,at *** order to better understand the physiological function of npy8br,especially in digestion,we used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology to generate npy8br-/-japanese medaka(Oryzias latipes).We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability,ultimately affecting their ***,npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes(npy and agrp).npy8br-/-medaka larvae fed for 10 d(10th day of feeding)still had incompletely digested brine shrimp(Artemia nauplii)in the digestive tract 8 h after feeding,the messenger RNA(mRNA)expression levels of digestion-related genes(amy,lpl,ctra,and ctrb)were significantly decreased,and the activity of amylase,trypsin,and lipase also significantly *** deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes(gh and igf1).Hematoxylin and eosin(H&E)sections of intestinal tissue showed that npy8br-/-medaka larvae had damaged intestine,thinned intestinal wall,and shortened intestinal *** far,this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.

关键词： Neuropeptide Y receptor Y8b(npy8br) Japanese medaka(Oryzias latipes) Knockout Feeding Digestion

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Origin and evolution of false brinelling and fretting corrosion under rolling conditions

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Friction 2024年第4期12卷 618-631页

作者： Xin jia.lu HUANG Miao SUN Wenbin LI National Institute of Metrology Beijing 100029China Yantai Tiancheng Machinery Co. Ltd.Yantai 264006China

Rolling bearings working in oscillating applications often suffer from false brinelling and fretting corrosion that limit their service *** present,the study of oscillation-induced failures is primarily dominated by a large number of sliding and bench ***,most of the previous work are not able to fully describe the two closely related but different failure *** this study,the origin and evolution of the two failures are thoroughly investigated under strictly controlled oscillating rolling *** results show that failure evolves from false brinelling to fretting corrosion under dry friction conditions regardless of the oscillation *** process is accompanied by a large area peeling and serious oxidation,and the oxygen content increases from 2.50 wt%to 21.22 wt%.Oxidation is a crucial factor in the evolution of false brinelling into fretting ***,the distribution of microwear and oxygen content depend on the oscillation *** lubrication conditions,the friction coefficient(COF)is smaller in the initial stage and led to local slip or transient instability of rolling ***,the size of the wear marks and COF increased and appeared to be larger than those under a dry friction ***,grease can separate the contact surfaces,only surface deformation owing to false brinelling and slight damage at the roughness level *** evolution of false brinelling and fretting corrosion is also closely related to residual *** brinelling often occurrs with residual stress accomulation,meanwhile,the residual stress increases from 120 to 300 *** peeling occurs,residual stress is *** failure pattern transformed from false brinelling to fretting corrosion and is accompanied by *** far,none of investigation is able to show satisfactory evolution of oscillating-induced failures,this study may contribute to more scientific understanding of rolling bearings against long-

关键词： oscillating false brinelling fretting corrosion wear residual stress

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Expression of cyclin-dependent kinase 9 is positively correlated with the autophagy level in colon cancer

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 314-330页

作者： Lei Zheng jia.lu Da-lu Kong Department of Colorectal Cancer Surgery National Clinical Research Center for CancerKey Laboratory of Cancer Immunology and Biotherapy of TianjinTianjin Key Laboratory of Digestive CancerKey Laboratory of Cancer Prevention and Therapy of TianjinTianjin’s Clinical Research Center for CancerTianjin Medical University Cancer Institute and HospitalTianjin 300060China Department of Infection Management National Clinical Research Center for CancerKey Laboratory of Cancer Immunology and Biotherapy of TianjinTianjin Key Laboratory of Digestive CancerKey Laboratory of Cancer Prevention and Therapy of TianjinTianjin’s Clinical Research Center for CancerTianjin Medical University Cancer Institute and HospitalTianjin 300060China

BACKGROUND Cyclin-dependent kinase 9(CDK9)expression and autophagy in colorectal cancer(CRC)tissues has not been widely ***9,a key regulator of transcription,may influence the occurrence and progression of *** expression of auto-phagy-related genes BECN1 and drug resistance factor ABCG2 may also play a role in *** normal physiological conditions,autophagy can inhibit tumorigenesis,but once a tumor forms,autophagy may promote tumor ***,understanding the relationship between autophagy and cancer,partic-ularly how autophagy promotes tumor growth after its formation,is a key motivation for this *** To investigate the relationship between CDK9 expression and autophagy in CRC,assess differences in autophagy between left and right colon cancer,and analyze the associations of autophagy-related genes with clinical features and *** We collected tumor tissues and paracarcinoma tissues from colon cancer patients with liver metastasis to observe the level of autophagy in tissues with high levels of CDK9 and low levels of *** also collected primary tissue from left and right colon cancer patients with liver metastasis to compare the autophagy levels and the expression of BECN1 and ABCG2 in the tumor and paracarcinoma *** The incidence of autophagy and the expression of BECN1 and ABCG2 were different in left and right colon cancer,and autophagy might be involved in the occurrence of chemotherapy *** analysis of the rela-tionship between the expression of autophagy-related genes CDK9,ABCG2,and BECN1 and the clinical features and prognosis of colorectal cancer showed that the high expression of CDK9 indicated a poor prognosis in colorectal *** This study laid the foundation for further research on the combination of CDK9 inhibitors and autophagy inhibitors in the treatment of patients with CRC.

关键词： Autophagy Tumorigenesis Tumor tissue Paracarcinoma tissue Expression Left/right colon cancer

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Triple signal amplification electrochemical sensing platform for Hg^(2+) in water without direct modification of the working electrode

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Frontiers of Environmental Science & Engineering 2024年第7期18卷 157-167页

作者： Liuyin Hu jia.ua Cui Tao lu Yalin Wang Jinping jia School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China International Copper Association Ltd.Shanghai 200020China

An ultrasensitive electrochemical biosensor to detect trace Hg^(2+)in environmental samples was developed utilizing nanogold-decorated magnetic reduced graphene oxide(MrGO-AuNPs),exonuclease III-assisted target cycle(Exo Ⅲ-ATC)and hybridization chain reaction(HCR)synergistic triple signal *** MrGO-AuNPs is a superior carrier for capture DNA(cDNA)and acts as magnetic media for automatic separation and *** innovative utilization of the magnetism and improved sensing efficiency obviates the need for direct modification and repeated polishing of the working ***,the three DNA hairpins(cDNA,methylene blue(MB)labeled HP1 and HP2)further contribute to biosensor specificity and *** cDNA captures Hgt,it activates Exo Ⅲ-ATC due to the formation of a sticky end in the DNA stem via thymine-Hig-thymidine(T-Hg^(2+)-T),this leads to the hydrolysis of self-folded DNA by Exo Ⅲ-ATC to form"key"DNA(kDNA).The kDNA subsequently initiates HCR,resulting in massive super-sandwich structures(kDNA-[HP1/HP2])carrying signaling molecules on MrGO-AuNPs,and this overall structure serves as a signal probe(SP).Leveraging magnetic adsorption,the SP was automatically adsorbed onto the magneto-glass carbon electrode(MGCE),generating an amplified *** biosensor's detection limit(LOD)was 3.14 pmol/L,far below the limit of 10 nmol/L for mercury in drinking water set by the US *** biosensor also showed excellent selectivity when challenged by interfering ions,and the results of its application in actual samples indicate that it has good potential for practical applications in environmental monitoring.

关键词： Electrochemical sensing Hg^(2+) Amplification Graphene Hybridization chain reaction ExonucleaseⅢ

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Interconnection of cellular autophagy and endosomal vesicle trafficking and its role in hepatitis B virus replication and release

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Virologica Sinica 2024年第1期39卷 24-30页

作者： jia.Li Yong Lin Xueyu Wang Mengji lu Institute for Virology University Hospital EssenUniversity of Duisburg-EssenEssen45122Germany Key Laboratory of Molecular Biology of Infectious Diseases(Chinese Ministry of Education) Chongqing Medical UniversityChongqing400016China The Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou310000China

Hepatitis B virus(HBV)produces and releases various particle types,including complete virions,subviral particles with envelope proteins,and naked *** studies demonstrate that HBV exploits distinct intracellular membrane trafficking pathways,including the endosomal vesicle trafficking and autophagy pathway,to assemble and release viral and subviral ***,we summarize the findings about the distinct roles of autophagy and endosomal membrane trafficking and the interaction of both pathways in HBV replication,assembly,and release.

关键词： Amphisome Autophagy Endosomal vesicle Hepatitis B virus(HBV)

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Mechanical properties and fracture surface roughness of thermally damaged granite under dynamic splitting

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Deep Underground Science and Engineering 2024年第1期3卷 103-116页

作者： Yijin Qian Peng jia.Songze Mao jia.iang lu School of Resources and Civil Engineering Northeastern UniversityShenyangChina

In order to understand the mechanical properties and the fracture surface roughness characteristics of thermally damaged granite under dynamic splitting,dynamic Brazilian splitting tests were conducted on granite samples after thermal treatment at 25,200,400,and 600℃.Results show that the dynamic peak splitting strength of thermally damaged granite samples increases with increasing strain rate,showing obvious strain‐rate *** increasing temperature,thermally induced cracks in granite transform from intergranular cracks to intragranular cracks,and to a transgranular crack *** induced damages reduce the dynamic peak splitting strength and the maximum absorbed energy while increasing the peak radial *** fracture mode of the thermally damaged granite under dynamic loads is mode Ⅱ splitting *** using the axial roughness index Z2 a,the distribution ranges of the wedge‐shaped failure zones and the tensile failure zones in the fracture surfaces under dynamic Brazilian splitting can be effectively *** radial roughness index Z_(2)^(r)is sensitive to the strain rate and *** shows a linear correlation with the peak splitting strength and the maximum absorbed energy and a linear negative correlation with the peak radial strain.Z_(2)^(r)can be used to quantitatively estimate the dynamic parameters based on the models proposed.

关键词： dynamic splitting fracture surface roughness granite strain rate thermal treatment

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Online task planning method of anti-ship missile based on rolling optimization

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Journal of Systems Engineering and Electronics 2024年第3期35卷 720-731页

作者： lu Faxing DAI Qiuyang YANG Guang jia.Zhengrong College of Weaponry Engineering Naval University of EngineeringWuhan 430033China

Based on the wave attack task planning method in static complex environment and the rolling optimization framework, an online task planning method in dynamic complex environment based on rolling optimization is proposed. In the process of online task planning in dynamic complex environment,online task planning is based on event triggering including target information update event, new target addition event, target failure event, weapon failure event, etc., and the methods include defense area reanalysis, parameter space update, and mission re-planning. Simulation is conducted for different events and the result shows that the index value of the attack scenario after re-planning is better than that before re-planning and according to the probability distribution of statistical simulation method, the index value distribution after re-planning is obviously in the region of high index value, and the index value gap before and after re-planning is related to the degree of posture change.

关键词： target allocation of anti-ship missile defense area rolling optimization task re-planning

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Preparation and characterization of Rb-doped TiO_(2) powders for photocatalytic applications

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Rare Metals 2024年第2期43卷 555-561页

作者： lu jia.Li-Mei Yang Wei Wang Song-Tao Huang Zheng Xu National Engineering Laboratory of Biohydrometallurgy General Research Institute for Nonferrous MetalsBeijing100088China

TiO_(2)nanoparticles doped with different concentrations of Rb were prepared by sol–gel *** crystal structures of samples were characterized by X-ray diffraction(XRD)and transmission electron microscopy(TEM).It was confirmed by the Rietveld refinement method that the as-prepared samples crystallize in anatase phase and Rb doping induces expansion and distortion of the crystal *** with the pure TiO_(2),the Rb-doped nanoparticles have smaller crystal size and better *** rate of methylene blue was used to evaluate the photocatalytic activity of Rb–TiO_(2).It indicated that doping Rb into TiO_(2)can effectively improve the photocatalytic activity of TiO_(2)and the mechanisms were discussed in *** using the sample doped with 0.20%Rb,methylene blue degraded with the fastest rate(97%within 60 min).

关键词： Rb doping TiO_(2) Photocatalysis Sol-gel

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Intelligent geochemical interpretation of mass chromatograms:Based on convolution neural network

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Petroleum Science 2024年第2期21卷 752-764页

作者： Kai-Ming Su Jun-Gang lu jia. Yu Zi-Xing lu Shi-jia.Chen Hubei Key Laboratory of Petroleum Geochemistry and Environment Wuhan430100HubeiChina College of Resources and Environment Yangtze UniversityWuhan430100HubeiChina School of Geoscience and Technology Southwest Petroleum UniversityChengdu610500SichuanChina Changqing Oilfield Company PetroChinaXi'an710021ShaanxiChina

Gas chromatography-mass spectrometry(GC-MS)is an extremely important analytical technique that is widely used in organic *** is the only approach to capture biomarker features of organic matter and provides the key evidence for oil-source correlation and thermal maturity ***,the conventional way of processing and interpreting the mass chromatogram is both timeconsuming and labor-intensive,which increases the research cost and restrains extensive applications of this *** overcome this limitation,a correlation model is developed based on the convolution neural network(CNN)to link the mass chromatogram and biomarker features of samples from the Triassic Yanchang Formation,Ordos Basin,*** this way,the mass chromatogram can be automatically *** research first performs dimensionality reduction for 15 biomarker parameters via the factor analysis and then quantifies the biomarker features using two indexes(*** and PMI)that represent the organic matter thermal maturity and parent material type,***,training,interpretation,and validation are performed multiple times using different CNN models to optimize the model structure and hyper-parameter setting,with the mass chromatogram used as the input and the obtained MI and PMI values for supervision(label).The optimized model presents high accuracy in automatically interpreting the mass chromatogram,with R2values typically above 0.85 and0.80 for the thermal maturity and parent material interpretation results,*** significance of this research is twofold:(i)developing an efficient technique for geochemical research;(ii)more importantly,demonstrating the potential of artificial intelligence in organic geochemistry and providing vital references for future related studies.

关键词： Organic geochemistry Biomarker Mass chromatographic analysis Automated interpretation Convolution neural network Machine learning

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New strategies for asymmetric photocatalysis: asymmetric organocatalytic/photoredox relay catalysis for efficient synthesis of polycyclic compounds containing vicinal amino alcohols

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Science China Chemistry 2024年第3期67卷 945-952页

作者： jia.lu Zhang Wen-Bo He Xiu-Qin Hu Peng-Fei Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China State Key Laboratory of Veterinary Etiological Biology College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000China MOE Frontiers Science Center for Rare Isotopes Lanzhou UniversityLanzhou 730000China

An asymmetric catalytic strategy in photocatalysis utilizing a chirality-induced approach through an organocatalytic/photoredox relay catalysis strategy is successfully achieved for the rapid construction of polycyclic compounds containing vicinal amino alcohols in a one-pot protocol. This methodology facilitates the efficient synthesis of diverse substituted polycyclic tetrahydroquinoline and benzofuran-derived vicinal amino alcohols, each containing five consecutive chiral centers, with high yields,excellent diastereoselectivities and enantioselectivities(up to 95% yield, >20:1 dr and 98% ee), under mild reaction conditions driven by sequential bifunctional squaramide organocatalyst-catalyzed [4+2] annulation and photocatalyst-catalyzed ketyl radical addition cyclization reaction process. Furthermore, investigations into the stereoselectivity mechanism and high-resolution mass spectrometry(HRMS) experiments on free radical trapping have provided evidence for elucidating the detailed mechanism of chirality-induced processes and chiral intermediate conversions in this procedure.

关键词： enantioselective photocatalysis asymmetric organocatalytic/photocatalysis relay catalysis five consecutive chiral centers chirality-induced strategy chirality vicinal amino alcohols

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