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Glioblastoma stem cells resistant to temozolomide-induced autophagy

Chinese Medical Journal 2009年第11期122卷 1255-1259页

作者： FU Jun LIU Zhi-gang LIU Xiao-mei CHEn Fu-rong SHI Hong-liu PAnG jesse Chung-sean nG Ho-keung CHEn Zhong-ping State Key Laboratory for Cancer Research in Southern China and Department of neurosurgery/neuro-oncology Cancer Center Sun Yat-sen University Guangzhon Guangdong 510060 China Department of Anatomical & Cellular Pathology Chinese University of Hong Kong Shatin N.T. Hong Kong China

Background Recent studies have demonstrated the existence of a small fraction of cells with features of primitive neural progenitor cells and tumor-initiating function in brain tumors. These cells might represent primary therapeutic target for complete eradication of the tumors. This study aimed to determine the resistant phenotype of glioblastoma stem cells （GSCs） to temozolomide （TMZ） and to explore the possible molecular mechanisms underlying TMZ resistance. Methods Freshly resected glioblastoma specimen was collected and magnetic isolation of GSCs was carried out using the Miltenyi Biotec CD133 Cell Isolation kit. The cytotoxic effect of TMZ on CD133^＋ and CD133^- glioblastoma cells was determined by using the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay. Autophagy-related proteins （Beclin-1, LC3 and Atg5） and cleaved caspase-3 （p17） were analyzed by Western blotting. Immunofluorescent staining was used to detect Atg5, glial fibrillary acidic protein （GFAP） and CD133 expression in glioblastoma cells. Statistical analysis was carried out using SPSS 10.0 software. For all tests, the level of statistical significance was set at P 〈0.05. Results CD133^＋ glioblastoma ceils exhibited neurosphere-like growth in vitro and high expression of CD133 stem cell marker. The growth-inhibiting rate in CD133- glioblastoma cells treated with 5 or 50 pmol/L TMZ was significantly higher than that in CD133^＋ glioblastoma cells （（14.36±3.75）% vs （2.54±1.36）% or （25.95±5.25）% vs （2.72±1.84）%, respectively, P 〈0.05）. Atg5, LC3-11 and Beclin-1 levels were significantly lower in CD133^＋ glioblastoma cells than those in autologous CD133^- cells after TMZ treatment （P 〈0.05）. Caspase-3 was mildly activated only in CD133^- glioblastoma cells after exposure to TMZ （P 〈0.05）. Immunofluorescent staining revealed elevated expression of Atg5 in GFAP^＋ cells following TMZ treatment. Conclusions The GSCs display strong capability of tumor＇s resistance to TMZ. This resis

关键词： glioblastoma neoplastic stem cells chemoresistance temozolomide

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High Temperature Oxidation of a Work Roll Grade High-Chromium White Cast Iron

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Journal of Iron and Steel Research International 2013年第10期20卷 122-129页

作者： J H Ramírez-Ramírez R Colás n F Garza-Montes-de-Oca Faculty of Mechanical and Electrical Engineering Autonomous University of Nuevo Leon

High-chromium white cast iron is a common material for the manufacture of the work rolls used in the fin- ishing stands in hot strip mills. The high temperature oxidation behaviour of as-cast and heat-treated high-chromium white cast iron was studied by gravimetric means at 650 ℃ in atmospheres that included dry air and a mixture of dry air and water vapour. For both environmental and material conditions, the oxidation rate of the alloys was represen ted by parabolic trends and the oxidation phenomenon was more obvious when the samples were oxidised in water vapour. Water vapour also influenced the chemical composition of the oxides formed in the alloy and under this condi tion, the oxide scale formed on the alloy contained the oxides FeCr2O4 , （Fe,Cr）3O4 and （Fe,Cr）2 O3. In contrast, the alloys exposed to dry air conditions only experienced the formation of FeCr2O4 and （Fe,Cr）2O3. It was found that the presence of water vapour and the chromium content of the alloys increased the oxidation rate by a mechanism that involved chromium depletion of the oxide as a result of hydroxide formation. The differences observed in the oxi- dation behaviour of the alloy are discussed in terms of the composition of the oxidant atmosphere and the metallurgi cal condition of the specimens.

关键词： white cast iron oxidation water vapour work roll

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High-performance modified uni-traveling carrier photodiode integrated on a thin-film lithium niobate platform

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Photonics Research 2022年第6期10卷 1338-1343页

作者： XIAnGWEn GUO LInBO SHAO LInGYAn HE KEVIn LUKE jesse MORGAn KEYE SUn JUnYI GAO TA-CHInG TZU YAnG SHEn DEKAnG CHEn BInGTIAn GUO FEnGXIn YU QIAnHUAn YU MASOUD JAFARI MARKO LOnčAR MIAn ZHAnG AnDREAS BELInG Department of Electrical and Computer Engineering University of VirginiaCharlottesvilleVirginia 22903USA John A.Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMassachusetts 02138USA HyperLight Corporation CambridgeMassachusetts 02139USA

Lithium niobate on insulator(LnOI)has become an intriguing platform for integrated photonics for applications in communications,microwave photonics,and ***,integrated devices including modulators,resonators,and lasers with high performance have been recently realized on the LnOI platform,high-speed photodetectors,an essential building block in photonic integrated circuits,have not been demonstrated on LnOI ***,we demonstrate for the first time,heterogeneously integrated modified uni-traveling carrier photodiodes on LnOI with a record-high bandwidth of 80 GHz and a responsivity of 0.6 A/W at a 1550-nm *** photodiodes are based on an n-down In GaAs/InP epitaxial layer structure that was optimized for high carrier transit time-limited *** integration was achieved using a scalable wafer die bonding approach that is fully compatible with the LnOI platform.

关键词： traveling performance modified

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Sprinkler Irrigation and Soil Tillage Practices in Sugarcane Plantations as Influenced by Soil Texture and Water Storage in northern Ivory Coast

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Journal of Life Sciences 2013年第3期7卷 293-301页

作者： Crepin B. Pene, Souleymane n'Diaye Chantal n'Guessan-Konan Department of R&D SUCAFCI/SOMDIAA Sugar Mills 33 rue des Brasseurs O1 P.O. Box 1967 Abidjan 01 Ivory Coast

Soil survey investigations were carried out in Ferk6 1 as well as Ferk6 2 sugar mills of northern Ivory Coast to determine soil texture and water storage capacity for sprinkler irrigation and tillage management. A 5-year term observation experiment on reduced tillage compared with conventional tillage was also conducted in Ferk6 1 over an irrigated cane crop of 28 ha for yield optimization purpose. Soil sampling was achieved after harvest or prior to re-plantation at five different spots along two transects over 30 cm depth in every farmland which covers about 30-40 ha with 432 m long cane rows as to get an average soil sample of 1.5-2 kg. Soil physical properties like texture and water retention curves were determined locally in the sugar company＇s soil laboratory. It came out that the majority of soils investigated was coarse-textured for about 64% in Ferk6 1 and 85% in Ferk6 2, with a lower to medium water storage capacity （70-89 mm） over 60 cm depth which corresponds to a readily available moisture less than 60 mm. Except for the sugarcane plant crop, no significant difference in cane yields resulting from tillage practices was observed over four consecutive cropping seasons. The yield decline from plant cane to first ratoon was very high under conventional tillage （-16 t/ha） compared with the reduced tillage （＋3 t/ha）. Even higher cane yield was obtained on the second ratoon （89 t/ha） compared with the conventional tillage （83 t/ha）.

关键词： Particle size distribution physical property water retention available moisture reduced tillage irrigation management.

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监测3例法布莱病患儿对酶替代疗法的临床及生化反应

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世界核心医学期刊文摘（儿科学分册） 2005年第3期1卷 31-32页

作者： mills K. Vellodi A. Morris P. E.Young 高蕊 Biochem. Endocrinol. and Metab. U. Institute of Child Health University College London 30 Guilford StreetWC1N 1EH London United Kingdom

法布莱病是一种X-连锁的糖鞘酯代谢异常,是由于溶酶体α-半乳糖苷酶A缺乏造成的,从而导致糖鞘酯在大部分内脏组织和体液内的溶酶体内不断沉积。继在成人身上所进行的临床试验获得成功后,最近两种α-半乳糖苷酶的重组酶制剂用于治疗该病... 详细信息

法布莱病是一种X-连锁的糖鞘酯代谢异常,是由于溶酶体α-半乳糖苷酶A缺乏造成的,从而导致糖鞘酯在大部分内脏组织和体液内的溶酶体内不断沉积。继在成人身上所进行的临床试验获得成功后,最近两种α-半乳糖苷酶的重组酶制剂用于治疗该病在欧洲获得了认可,同时开始应用于儿童法布莱病的治疗。笔者观察了3例接受酶替代治疗(agalsidase beta,Fab- razyme,1 mg/kg)2年的患儿临床表现及血浆和尿液的酰基鞘鞍醇三己糖浓度的变化。其中1例患儿在接受治疗后所有的临床和生化指标均得到了迅速的改善,而且疗效持续。另外2例患儿是兄弟,在接受治疗1年后其临床指标没有明显改善,而其血浆和尿液的酰基鞘鞍醇三己糖浓度虽较治疗前有所降低,但仍显著高于正常值。因为没有阻断或中和抗体的迹象,故在治疗74周时将其剂量上调至2 mg/kg。治疗2年后其疼痛指数有所改善,但并不伴有血浆和尿液的酰基鞘鞍醇三己糖浓度的降低。结论:监测血浆和尿液的酰基鞘鞍醇三己糖浓度并不能准确反映所有接受酶替代治疗患者治疗的进展情况。本文报道的两兄弟,其主观的临床改善就较客观的生化指标更能反映出疗效。

关键词：法布莱病酶替代疗法己糖溶酶体临床改善生化反应生化指标 α-半乳糖苷酶疼痛指数临床试验

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循环中的血管生成因子与胎盘早剥

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世界核心医学期刊文摘（妇产科学分册） 2006年第11期2卷 61-62页

作者： Signore C. mills J.L. R.J. Levine 柳蕴 nIH/ nICHD Building 6100 Bethesda MD 20892 United States Dr.

OBJECTIVE: Abnormalities in circulating angiogenic factors have been reported in diseases of abnormal placentation, such as preeclampsia and intrauterine growth restriction. Our objective was to determine whether circulating angiogenic factors are altered in another placental vascular disease, abruptio placentae. METHODS: In a nested case-control study of nulliparous pregnancies, we examined levels of placental growth factor (PlGF) and soluble fms-like tyrosine kinase 1 (sFlt-1) in serum collected prospectively from 31 women who later developed placental abruption and from 31 normal control subjects. All serum specimens were collected before the onset of hypertension or abruption and before labor or delivery. Serum angiogenic factors were compared within 3 gestational age windows: early (20 weeks or less), middle (21- 32 weeks), and late (33 weeks or more) pregnancy. RESULTS: During early pregnancy women who developed placental abruption had lower PlGF and higher sFlt- 1 concentrations and higher sFlt- 1/PlGF ratios than women with normal pregnancies. In mid- pregnancy these differences became greater, reaching statistical significance for PlGF concentration (431 versus 654 pg/mL, P nd the sFlt- 1/PlGF ratio (25.3 versus 2.5, P n the women with placental abruption were subdivided into those who did (n=10) and those who did not (n=21) develop preeclampsia or gestational hypertension, significant alterations in angiogenic factors were noted only in women who later developed hypertension in pregnancy. Among these women, PlGF concentrations were decreased in mid-pregnancy (160 versus 723 pg/mL, P nd the mid-pregnancy sFlt- 1/PlGF ratio was increased (70.1 versus 2.3, P=.001). COnCLUSIOn: Serum levels of the proangiogenic factor PlGF were decreased, and those of the antiangiogenic ratio sFlt- 1/PlGF were increased in nulliparous women who subsequently devel-oped hypertension and placental abruption.

关键词：胎盘早剥血管生成因子胎盘生长因子胎盘形成妊娠中期妊娠高血压胎儿宫内生长先兆子痫血管生

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面向21世纪的板卷生产技术

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钢铁 2004年第6期39卷 75-78页

作者： n.J.Champion 奥钢联(英国)工业有限公司

与当今高效、低成本的热带钢生产相反 ,中厚板轧机一般具有产量低、非连续、效率低等特点。在此方面 ,轧机设备的开发和操作效率的提高未能同对于产品质量和性能指标的要求保持同步。目前这一局面正在改变。比如 ,板卷轧机配置能够以低... 详细信息

与当今高效、低成本的热带钢生产相反 ,中厚板轧机一般具有产量低、非连续、效率低等特点。在此方面 ,轧机设备的开发和操作效率的提高未能同对于产品质量和性能指标的要求保持同步。目前这一局面正在改变。比如 ,板卷轧机配置能够以低生产成本而达到更大产能。对相关工艺布置和技术方案进行描述。

关键词：中厚板轧机板卷轧机热机控轧

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左右侧Mallinckrodt双腔管临床性能相同

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麻醉与镇痛 2009年第4期5卷 34-39页

作者： jesse M. Ehrenfeld John L. Walsh Warren S. Sandberg 王娟(译) 李文志(校) Harvard Medical School Department of Anesthesia and Critical Care Massachusetts General Hospital Boston Massachusetts 不详

背景以往的观点认为因左侧双腔管不易发生错位所以比右侧双腔管更安全。如果这种观点正确，那么胸科手术中，右侧双腔管的低氧血症、高碳酸血症、高气道压的发生率和严重程度直比左侧双腔管高。方法我们采用自动化麻醉信息管理系统回顾... 详细信息

背景以往的观点认为因左侧双腔管不易发生错位所以比右侧双腔管更安全。如果这种观点正确，那么胸科手术中，右侧双腔管的低氧血症、高碳酸血症、高气道压的发生率和严重程度直比左侧双腔管高。方法我们采用自动化麻醉信息管理系统回顾性分析了2003年4月15日到2004年12月31日的胸外科手术麻醉记录？该系统每隔30秒自动记录脉搏氧饱和度、呼气末二氧化碳浓度、气道峰压，也记录手术的部位和双腔管的位置。我们比较胸科麻醉医师使用左右侧双腔管的频率。继而，测定肺脏和胸壁手术患者的低氧血症（SpO2〈90％）、高碳酸血症（RtCO2〉45mmHg）和高气道压（气道峰压〉35cmH2O）的发生率、持续时间和严重程度，采用标准统计学方法比较各组的例数及数据。结果右侧双腔管（n=241）和左侧双腔管（n=450）广泛应用于对侧手术。在低氧血症、高碳酸血症或高气道压的发生率或持续时间上，两种方式没有显著差别。在左肺通气的患者中，EtCO2轻厦升高但差异有显著统计学意义。结论在手术期间，当低氧血症、高碳酸血症以及高气道压的发生率和严重程度作为安全标准时，比较来自同一厂家的两种双腔管的数据，本研究结果否定了左侧双腔管比右侧双腔管安全的推测。

关键词：双腔管右侧呼气末二氧化碳浓度高碳酸血症统计学方法低氧血症安全标准性能

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在固相pH梯度4~7范围内海洛因成瘾者血浆蛋白质组分析(英文)

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中国药理学与毒理学杂志 2008年第4期22卷 241-249页

作者：周海涛朱红刘建军徐靖李岭贾少微刘鹏程北京大学深圳医院广东深圳518036 深圳市福田区慢性病医院广东深圳518048 深圳市疾病预防控制中心广东深圳518020 深圳市公安强制戒毒所广东深圳518019 中国医科大学医学遗传教研室辽宁沈阳110001

目的比较鉴定海洛因成瘾者和正常人血浆蛋白质组差异,为研究海洛因成瘾相关血浆蛋白提供线索。方法海洛因成瘾者（n=5）和正常对照者（n=5）血浆蛋白经剔除白蛋白和免疫球蛋白IgG后,以固相pH梯度4-7胶条等电聚焦为第一向,SDS聚丙烯酰胺... 详细信息

目的比较鉴定海洛因成瘾者和正常人血浆蛋白质组差异,为研究海洛因成瘾相关血浆蛋白提供线索。方法海洛因成瘾者（n=5）和正常对照者（n=5）血浆蛋白经剔除白蛋白和免疫球蛋白IgG后,以固相pH梯度4-7胶条等电聚焦为第一向,SDS聚丙烯酰胺凝胶电泳为第二向,进行蛋白双向电泳。图像分析软件Image Master Elit 5.0分析蛋白质2维图谱。手工挖取组间相差1.5倍的差异点,串联质谱分析鉴定。结果每张图谱平均检测到350±21个蛋白（亚基）斑点,其中5个蛋白点在2组图谱中差异1.5倍以上,鉴定结果分别为γ纤维蛋白原、人α1B糖蛋白、α1-抗胰蛋白酶原、视黄醇结合蛋白载体蛋白四聚体单体和铜蓝蛋白。结论海洛因成瘾者血浆与正常人血浆对比存在蛋白质组差异。某些差异蛋白可能与海洛因成瘾造成的神经损伤相关。

关键词：海洛因依赖光谱分析,质量血浆蛋白质组电泳,凝胶,双向

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Unraveling one billion years of geological evolution of the southeastern Amazonia Craton from detrital zircon analyses

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Geoscience Frontiers 2022年第5期13卷 129-152页

作者： Camille Rossignol Paul Yves Jean Antonio Francesco narduzzi Eric Siciliano Rego Lívia Teixeira Romário Almeida de Souza Janaína n.Ávila Marco A.L.Silva Cristiano Lana Ricardo I.F.Trindade Pascal Philippot Departamento de Geofísica Instituto de AstronomiaGeofísica e Ciências Atmosféricas da Universidade de São PauloRua do Matão1226-Cidade UniversitáriaSão Paulo 05508-090Brazil Institut de Physique du Globe de Paris Universitéde ParisCNRSParisFrance Géosciences Montpellier Universitéde MontpellierCNRSUniversitédes AntillesMontpellierFrance Instituto de Geociências Universidade de São PauloRua do Lago562-Cidade UniversitáriaSão Paulo 05508-080Brazil Instituto de Geociências e Engenharias Universidade Federal do Sul e Sudeste do Pará–UNIFESSPAFolha 17Quadra 04Lote especialNova MarabáMarabáPará68505-080Brazil Research School of Earth Sciences The Australian National University142 Mills RoadCanberraACT 2601Australia Applied Isotope Research Group Departamento de GeologiaEscola de MinasUniversidade Federal de Ouro PretoRua Diogo de Vasconcelos12235400-000 Ouro PretoMGBrazil

Despite representing one of the largest cratons on Earth,the early geological evolution of the Amazonia Craton remains poorly known due to relatively poor exposure and because younger metamorphic and tectonic events have obscured initial *** this study,we investigated the sedimentary archives of the Carajás Basin to unravel the early geological evolution of the southeastern Amazonia *** Carajás Basin contains sedimentary rocks that were deposited throughout a long period spanning more than one billion years from the Mesoarchean to the *** oldest archives preserved in this basin consist of a few ca.3.6 Ga detrital zircon grains showing that the geological roots of the Amazonia Craton were already formed by the *** the Paleoarchean or the early Mesoarchean(n was large and rigid enough to sustain the formation and preservation of the Rio novo Group greenstone ***,during the neoarchean,at ca.2.7 Ga,the southeastern Amazonia Craton witnessed the emplacement of the Parauapebas Large Igneous Province(LIP)that probably covered a large part of the craton and was associated with the deposition of some of the world largest iron *** emplacement of this LIP immediately preceded a period of continental extension that formed a rift infilled first by iron formations followed by terrigenous *** major change of sedimentary regime might have been controlled by the regional tectonic evolution of the Amazonia Craton and its emergence above *** the Paleoproterozoic,at ca.2.1 Ga,the Rio Fresco Group,consisting of terrigenous sediments from the interior of the Amazonia Craton,was deposited in the Carajás *** that time,the Amazonian lithosphere could have either underwent thermal subsidence forming a large intracratonic basin or could have been deformed by long wavelength flexures that induced the formation of basins and swells throughout the craton under the influence of

关键词： Carajás Basin Chemical Abrasion–LA-ICP-MS analyses Age distribution comparison Parauapebas Large Igneous Province Transamazonian orogeny

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