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大鼠感染肝片吸虫后红细胞过氧化损伤的实验研究

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上海交通大学学报（农业科学版） 2001年第1期19卷 30-32,61页

作者：顾有方毛鑫智沈永林 J.Gonzalez-gallego 南京农业大学动物医学院南京210095 西班牙莱昂大学生理药理毒理系莱昂24071

本文通过大鼠感染肝片吸虫复制感染模型,研究肝片吸虫诱导红细胞脂质过氧化损伤.将60只大鼠随机分成试验组(n=30)和对照组(n=30),试验组大鼠一次口服25个囊蚴,对照组不感染,于感染前和感染后连续9周采血制备样本,检测GsH-Px、sOD、GsT... 详细信息

本文通过大鼠感染肝片吸虫复制感染模型,研究肝片吸虫诱导红细胞脂质过氧化损伤.将60只大鼠随机分成试验组(n=30)和对照组(n=30),试验组大鼠一次口服25个囊蚴,对照组不感染,于感染前和感染后连续9周采血制备样本,检测GsH-Px、sOD、GsT活性和MDA含量.结果表明大鼠感染肝片吸虫后,溶血液中GsH-Px活性的变化不明显,总体变化趋势是升高,但整个实验期,两组差异不显著(P＞0.05);ssT和GsT活性的变化不明显;MDA含量总体变化趋势是升高,在第3、7周时与对照组差异显著(P＜0.05),证实肝片吸虫感染后,红细胞发生了脂质过氧化作用,自由基参与了肝片吸虫病的发展过程.

关键词：大鼠肝片吸虫红细胞脂质过氧化自由基膜损伤机理

实验感染肝片吸虫山羊和大鼠血清花生四烯酸代谢物的变化研究

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动物医学进展 2002年第1期23卷 61-63页

作者：顾有方毛鑫智沈永林 J.Gonzalez-gallego 安徽技术师范学院安徽凤阳233100 南京农业大学动物医学院江苏南京210095 西班牙莱昂大学生理药理毒理系莱昂24071

实验一选择９只健康、６～１０月龄雄性白山羊，经粪便学检查和Ｄｏｔ－ＥＬＩＳＡ检测，确认无肝片吸虫感染，随机分成感染组（ｎ＝５）和对照组（ｎ＝４），试验组每只一次口服接种１５０个肝片吸虫囊蚴，每周定时从颈静脉采集感染前... 详细信息

实验一选择９只健康、６～１０月龄雄性白山羊，经粪便学检查和Ｄｏｔ－ＥＬＩＳＡ检测，确认无肝片吸虫感染，随机分成感染组（ｎ＝５）和对照组（ｎ＝４），试验组每只一次口服接种１５０个肝片吸虫囊蚴，每周定时从颈静脉采集感染前（０周）和感染后２３周山羊血液一次，分离血浆；实验二将６０只大鼠随机分成感染组（ｎ＝３０）和对照组（ｎ＝３０），感染组大鼠一次口服２５个囊蚴，对照组不感染，于感染前（０周）和感染后（１，３，５，７，９周）采集血样。分别测定试验组和对照组羊和鼠花生四烯酸代谢物６－酮－前列腺素Ｆ１α（６－ｋｅｔｏ－ＰＧＦ１α）和血栓素Ｂ２（ＴＸＢ_２）的变化。结果表明肝片吸虫感染后，山羊血浆中ＴＸＢ_２水平变化，在１～３周与对照组相比无显著差异，从第５周开始到实验结束，试验组血浆中ＴＸＢ_２水平均显著或极显著地高于对照组。大鼠血栓素水平在感染后前５周逐渐下降，以后急剧上升，至实验结束时显著高于对照组；而整个实验期间，两种动物血浆中６－ｋｅｔｏ－ＰＧＦ１α水平变化不显著。提示肝片吸虫感染后，机体发生多种功能的改变，花生四烯酸代谢物参与了肝片吸虫病的发展过程。

关键词：山羊大鼠肝片吸虫花生四烯酸代谢物实验感染血清

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肝片吸虫感染对山羊机体无机盐含量的影响

安徽技术师范学院学报 2001年第4期15卷 25-27页

作者：顾有方朱道仙毛鑫智沈永林 J.Gonzalez-gallego 安徽技术师范学院安徽凤阳233100 南京农业大学动物医学院江苏南京210095 西班牙莱昂大学生理药理毒理系莱昂24071

选择 9只 6 - 10月龄的健康雄性白山羊 ,经粪便检查和DotBLIsA检测 ,确认无肝片吸虫感染 ,随机分成感染组 (n =5 )和对照组 (n =4 ) ,试验组每只一次口服接种 15 0个肝片吸虫囊蚴。每周定时从颈静脉采集感染前 0周和感染后 14周血液一... 详细信息

选择 9只 6 - 10月龄的健康雄性白山羊 ,经粪便检查和DotBLIsA检测 ,确认无肝片吸虫感染 ,随机分成感染组 (n =5 )和对照组 (n =4 ) ,试验组每只一次口服接种 15 0个肝片吸虫囊蚴。每周定时从颈静脉采集感染前 0周和感染后 14周血液一次 ,分离血清 ,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量 ,旨在研究感染肝片吸虫对山羊机体无机盐含量的影响。结果表明感染后山羊血清中的钾、钠、钙、镁、磷、氯的含量均成波动变化 ,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化 ,这与虫体感染后机体的代谢发生改变有关。

关键词：山羊感染肝片吸虫血清无机盐含量

用空气-乙炔火焰流动注射分析间接原子吸收测定铀

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世界核地质科学 1988年第3期 69-73页

作者： P.Martinez-Jiménez M.gallego M.Valcárcel 魏澎

本文提出间接测定铀的方法,它基于少量的铀使铁的原子信号增强,允许间接测定铀的浓度范围为0.05—1.0mg/L(RsD±0.46％)。流动注射系统的应用是很简单的,对于注入体积54μL时,采样频率为150/h。对55种外来物质对测定铀的影响进行了研究。

关键词：原子吸收信号增强燃烧器高度乙炔火焰流动注射分析空气工业气体

Ancient two expansions of GYPsY-LTR transposons underlie the large genome of Norway spruce

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Ancient two expansions of GYPSY-LTR transposons underlie the...

Inguinal Lymphadenectomy Assessment Associated with Penile Carcinoma Undergone New strategies for Nodal staging

2016年全国植物生物学大会

作者： Israel Ausin Jixian Zhai suhua Feng Peggy Kuo Elise L.Jacobsen Javier gallego-Bartolome Nathaniel R.street steven E.Jacobsen Haifeng Wang Fujian Province Key Laboratory of Plant Virology Institute of Plant VirologyFujian Agriculture and Forestry University

DNA methylation plays important roles in many biological processes,such as silencing of transposable elements,imprinting and regulating gene expression. Many studies of DNA methylation have shown its essential roles in angiosperms(flowering plants). However,few studies have examined the roles and patterns of DNA methylation in gymnosperms. Here,we present the first genome-wide singlebase resolution methylation maps of Norway spruce(Picea abies) from both needles and culture tissues through whole genome bisulfite sequencing. Dense methylation was observed globally across the whole genome,which differed from other plants that only had increased methylation present in their pericentromeric regions. On average,DNA methylation levels of CG and CHG were much higher than most other model plants. Consistent with other plants,CHH methylation was relatively low and at least one copy of Rd DM pathway genes was found in Norway spruce. In comparison to needles,culture tissue showed lower CG and CHG methylation levels but higher CHH methylation,which is inconsistent with other regenerated tissue studies. similar to other genic DNA methylation studies,global DNA methylation levels were also positively correlated with genome size in plants. Two rounds of GYPsY-type LTR expansions were identified in the history of Norway spruce. This could partly explain the large genome size of the gymnosperms,like Norway spruce,which differ from most of angiosperms in that they have not undergone recent whole genome duplication(WGD). Altogether,the secret of the large genome of Norway spruce could not be explained by reduced DNA methylation levels and reactivation of transposons or whole genome duplication,but it can be partly explained by ancient GYPsY expansions.

关键词： DNA methylation Norway spruce whole genome duplication

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Case Reports in Clinical Medicine 2014年第7期3卷 410-415页

作者： Victor J. Ovejero Gomez L. Martin Cuesta F. Martinez Bretones T. gallego Bellido L. A. Asensio Lahoz J. Villalba Torre A. Ingelmo setien J. M. Bajo Arenas Department of Radiology Hospital Sierrallana Cantabria Spain Department of surgery Hospital Sierrallana Cantabria Spain Department of Urology Hospital Sierrallana Cantabria Spain Universidad Autónoma Madrid Spain

Penile carcinoma is an uncommon urological tumour and provides an opportunity for curation on early stage of the disease. Nodal metastases are one of the most important prognostic factors for survival although detection of inguinal adenopathies could be related with an inflammatory or infectious etiology. A suspicion of bilateral metastasic nodal involvement should be taken with caution. Radical inguinal lymphadenectomy has been associated to a great deal of complications. several anatomical studies have reported the true lymphatic drainage pathways in order to reduce the area of groin dissection. Nonetheless, a prophylactic modified inguinal lymphadenectomy should not be a systematic surgical procedure in all patients due to morbidity and questioned usefulness when there are not nodal metastasis. Classical imaging studies have a limited contribution to the diagnosis of lymph node metastasis. Nowadays, lymph node involvement may be diagnosed both minimally invasive and noninvasive techniques, such as dynamic sentinel lymph node biopsy in intermediate and high risk patients with nonpalpable lymph nodes, and fine needle aspiration biopsy in cases with palpable nodes. Their high effectiveness has facilitated the radical pelvic or inguinal lymphadenectomy that is only performed when there is histological confirmation of nodal involvement. A new video endoscopic technique has been developed at present to reduce postoperative complications although prospective studies are needed to assess outcomes. The appearance of adenopathies after surgical treatment of the primary tumour could be supported at the same *** inguinal lymphadenectomy should be carried out in selected patients to support a benefit on early stages with an extended survival.

关键词： Carcinoma Penis surgical Treatment Lymphadenectomy Nodal staging

第4级亲属的常染色体隐性白介素-1受体相关激酶-4缺乏

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世界核心医学期刊文摘（儿科学分册） 2006年第A10期2卷 31-32页

作者： Cardenes M. von Bernuth H. garca- saavedra A. C. Rodrguez- gallego 郭战宏 Departments of Immunology and Microbiology Dr. Negrn Universitary Hospital Gran Canaria SpainDr.

We report a kindred with autosomal recessive interleukin- 1 receptor- associated kinase 4 (IRAK- 4) deficiency in 3 fourthdegree relatives. A diagnosis of IRAK- 4 deficiency should be considered in families with invasive bacterial disease, even if the individuals affected are only distantly related, which falsely suggests multigenic or dominant inheritance with low penetrance.

关键词：常染色体隐性外显率细菌感染性疾病显性遗传名第多基因遗传侵袭性

Therapeutic Management in Proximal Tracheal Rupture during Total Thyroidectomy

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Case Reports in Clinical Medicine 2014年第6期3卷 382-386页

作者： Victor J. Ovejero-Gomez M. V. Bermudez-garcia J. L. Lamfus-Prieto T. gallego-Bellido J. Villalba-Torre A. Ingelmo-setien J. M. Bajo-Arenas Department of Anaesthesiology Hospital Sierrallana Cantabria Spain Department of surgery Hospital Sierrallana Cantabria Spain Universidad Autónoma Madrid Spain

Tracheal disruption is a life-threatening rare complication of total thyroidectomy that it should be prevented. The own-patient risk factors, procedure of tracheal intubation and a meticulous surgical technique are three main keys to keep in mind. Both medical and surgical management of this injury depends on its size, location and patient’s symptoms although neither of them prevents late complications. An early diagnosis could improve with prognosis although it is mandatory to perform either respiratory functional or imaging study in order to assume its healing. When the tracheal laceration is diagnosed during the surgery, we should take care with a continuous monitoring of the patient’s vital signs. A good oxygenation will be succeeded in high volume and low pressure with endotracheal tube distal to the lesion. We advice placing a tube drain near the disruption to prevent emphysema and an early extubation to avoid an ischaemic damage of the mucosa. A postoperative suspicion of tracheal rupture could demand reintubation with the patient in full relaxation and an examination by CT-scan or fiberscope before deciding a simple supportive therapy. We present our therapeutic experience in a female patient who suffered from tracheal injury during total thyroidectomy and describe a review of literature.

关键词： Tracheal Rupture Iatrogenic Thyroidectomy Treatment

Colorectal Cancer surgery in Extreme Elderly Population

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Journal of Cancer Therapy 2015年第1期6卷 12-20页

作者： Javier gallego Plazas Elena Asensio Juan C. Navalon Inmaculada Lozano Jose M. Navarro Guillermo Ricote Montse Olcina Almudena Cotes Henry M. Ore Alejandra Magdaleno Maria C. Ors Maria J. Escudero Miguel A. Morcillo servicio de Oncología Médica Hospital General Universitario de Elche-Hospital Vega Baja Alicante Espana servicio de Cirugía General Hospital Vega Baja Alicante Espana

Background: Colorectal cancer surgery in extreme elderly population (380 years) is a growing problem and a subject in question. Experiences and results in this scenario are limited. Methods: Medical records of patients?380 years old hospitalized in General surgery Department in Vega Baja Hospital between January 1st 2008 and December 31st 2012 were retrospectively reviewed. Patients diagnosed with colorectal cancer (CRC) were selected. Data regarding comorbidities, treatment performed, complications and survival were collected. Results: 95 patients (74 colon, 21 rectum) were identified: median age 83.0 years (80 - 93), 64.2% males. 93.7% of patients were operated on: median hospital and post-operative stay of 11 and 9 days, respectively;curative intention surgery 82.1%. 4.2% of patients were treated with interventionist procedures, and 2.1% of patients in a conservative way. 17.9% and 4.2% of patients came up with early and late complications, respectively. Early and late mortality occurred in 9.5% and 3.2% of patients, respectively. By March 2014, with a median follow up of 43.8 months, median overall survival for colorectal cancer patients was 2.7 years (95%IC, 2.0 - 3.2). Univariaye Cox Regresion analysis revealed the presence of cardiomyopathy (p = 0.024), the presence of chornic kidney disease (p = 0.025), the presence of comorbidities (vs absence) (0.026), the number of comorbidities (0.034), type of admission (p = 0.001), treatment with surgery (p = 0.001) and the incidence of early (p = 0.004) or late complications (p = 0.023) associated to overall survival with statically significance. Multivariate Cox Regression analysis showed number of comorbidities (HR = 1.104;95%CI: 0.851 - 1.431;p = 0.456), treatment with surgery (HR = 4.928;95%CI: 1.815 - 13.385;p = 0.002), programmed admission into hospital (HR = 2.316;95%CI: 1.298 - 4.133;p = 0.004), and the incidence of late complications (HR = 4.629;95%CI: 1.279 - 16.750;p = 0.020) independently associated with o

关键词： Colorectal Cancer surgery Elderly