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Fluorescence Resonance Energy Transfer Competitive Binding Assay for Secretin Receptor （Class B-GPCR）

Journal of Pharmacy and Pharmacology 2014年第5期2卷 295-303页

作者： Vijayalakshmi Senthil jerome Leprince David Vaudry Billy Kwok Chong Chow Department of Zoology University of Hong Kong Hong Kong Department of Federative Institute for Multidisciplinary Research on Peptides (IFRMP23) University of RouenMont-Saint-Aignan 76821 France

Human secretin is responsible for carrying a number of physiological functions including energy and water homeostasis, thus making secretin receptor a promising target for drug development. For GPCRs （G protein-coupled receptors）, radioactive ligands are usually used in conventional binding assays to characterize the binding affinities of the ligands. An alternative non-hazardous fluorescence based binding assay is lucrative over the radio-ligand assays. Here, we have developed a FRET （fluorescence resonance energy transfer） competitive binding assay for human secretin receptor. The receptor gene sequence is cloned in the SNAP （single nucleotide amplified polymorphisms） tag-plasmid and expressed in CHO （chinese hamster ovary）-K1 cells. Its expression and function is confirmed with immunofluorescence localization and receptor activation. The receptor and the ligand are labeled with fluorescent donor （Tb） and acceptor （Alexa488）. FRET signals are produced when the labeled ligand is bound to the receptor and the same drop when it is displaced by the test compounds. The saturation concentration of the receptor labeling is 100 nM, and the ligand Kd value is 500 nM. At these concentrations, the IC50 of unlabeled secretin is 1.63 4- 3.55 nM. Additionally, few class-B ligands are screened and hold good correlation with traditional radio-ligand assay. Henceforth, this FRET binding assay can be efficiently used as a primary screening tool for peptide analogs.

关键词： Class-B GPCR FRET binding assay human secretin receptor peptide analogs secretin.

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Developing an effective industrial cluster from strawberry farms： A case of Dahu Township in Taiwan

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Chinese Business Review 2010年第11期9卷 34-39页

作者： Yu-Ning Hu jerome Chih-Lung Chou Chia-Liang Hung Global Hakka Research Center National United University Miaoli 360 Taiwan Department of Information Management Hwa-Hsia Institute of Technology Taipei 235 Taiwan Department of Information Management National Chi-Nan University Puli 545 Taiwan

Dahu, a small township in northern Taiwan, is famous for its produce of strawberries. Near Dahu, there are some attractions, such as hot springs, native Taiwan Residents tribes and a national park named Shei-pa. In order to better support more small businesses in this area, the local government is eager to promote an industrial cluster of agriculture and tourism based on the well-developed strawberry farms. In this research, the authors investigated 200 strawberry farmers to examine forming factors of the cluster, and effects of cluster on organizational performance. The findings are firstly, capital resource is regarded as the most effective forming factor of the cluster. Secondly, institutionalization, interaction mechanism and knowledge flow positively intermediate the clustering effects on organizational performance. These findings have important implications for the local government and the businesses as well.

关键词： industrial cluster organizational behavior organizational performance

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In vivo testing of a bone graft containing chitosan, calcium sulfate and osteoblasts in a paste form in a critical size defect model in rats

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Journal of Biomedical Science and Engineering 2009年第1期2卷 24-29页

作者： jerome G. Saltarrelli Jr. Debi P. Mukherjee Louisiana State University Health Sciences Center Department of Orthopaedic Surgery 1501 Kings Highway Shreveport Louisiana 71130

Bone loss associated with musculoskeletal trauma or metabolic diseases often require bone grafting. The supply of allograft and auto-graft bones is limited. Hence, development of synthetic bone grafting materials is an active area of research. Chitosan, extracted from chitin present in crawfish shells, was tested as a de-livery vehicle for osteoblasts in a 2-3 mm size defect model in rats. Twenty-seven male Lewis rats, divided into three groups with sacrifice intervals of 3, 6 &9 months were used. In the experimental samples, a critical size defect was filled with chitosan bone graft paste and fixed with a plate, while in the operated control group, a critical size defect was repaired only by a plate (no paste was applied). An unoperated control group was also included. Bone growth was evaluated histologically by examining undecal-cified and decalcified stained sections. The fe-murs were also examined non-destructively by micro-computed tomography (礐T). Defects filled with chitosan bone graft paste demon-strated superior healing across all time periods compared to unfilled defects as examined by histology and micro-computed tomography. Crawfish chitosan has successfully been used as a cell delivery system for osteoblasts for use as a synthetic bone graft material.

关键词： Chitosan Synthetic Bone Graft Cell Delivery Histology Animal Model Running Head: Chitosan Based Synthetic Bone Graft Material

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The association between radiological spreading pattern and clinical outcomes in necrotizing external otitis

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Journal of Otology 2022年第3期17卷 156-163页

作者： W.Leentje van der Meer Ahmed B.Bayoumy Josje J.Otten jerome J.Waterval Henricus P.M.Kunst Alida A.Postma Department of Radiology&Nuclear Medicine Maastricht University Medical Centerthe Netherlands Department of Otorhinolaryngology and Head and Neck Surgery Maastricht University Medical Centerthe Netherlands Department of Otorhinolaryngology and Head and Neck Surgery Radboud Institute for Health SciencesRadboud University Medical Centerthe Netherlands School for Mental Health&Neuroscience Maastricht UniversityMaastrichtthe Netherlands Dutch Academic Alliance Skull Base Pathology Maastricht University Medical Center+Radboud University Medical CenterMaastricht/Nijmegenthe Netherlands

Objectives: Necrotizing external otitis(NEO) is a rare infectious disease of the skull base. The purpose of this study was to determine whether clinical outcomes of NEO can be correlated to different infectious spread ***: Retrospective chart review from 2010 to 2019 with NEO patients, who were divided into two cohorts: single spreading patterns(group A) or complex spreading patterns(group B) as diagnosed by *** symptoms, diagnostic and treatment delay, course of disease, complications, and duration of antibiotic exposure were retrospectively collected from patient ***: 41 NEO patients were included, of which 27 patients belonged to group A(66%). The diseaserelated mortality rate was 12.2% among the entire cohort, no differences were found between group A and B. Higher rates of ***(42.9% vs 14.8% P = 0.047) and N. IX palsies were found in group B compared to group A(28.6% vs 3.7%, P = 0.039). The median duration of antibiotic use was significantly different for a complex spreading pattern, clinical recovery and hospitalizations. Complications were associated with higher diagnostic delay and with a complex spread pattern. The median duration of follow-up was 12.0(IQR 6.0-19.5) ***: NEO is a severe disease, with significant mortality and morbidity(cranial nerve palsies). The radiological spread pattern may assist in predicting clinical outcome. Furthermore, complex spread patterns are associated with higher rates of clinical nerve palsies(N. VII and ***), complications, surgery rates and longer duration of antibiotic use. Diagnostic delay was associated with mortality, complications and facial palsies.

关键词： Necrotizing external otitis Malignant external otitis Skull base osteomyelitis Clinical manifestation Spreading routes Antibiotic exposure Facial nerve palsy

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Heavy Metal Pollution in Soil and Water in Some Selected Towns in Dunkwa-on-Offin District in the Central Region of Ghana as a Result of Small Scale Gold Mining

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Journal of Agricultural Chemistry and Environment 2014年第2期3卷 40-47页

作者： jerome D. A. Kpan Boadu Kwesi Opoku Anukwah Gloria College of Optical-Electrical and Computer Engineering University of Shanghai for Science and Technology Shanghai China Department of Chemistry University of Cape Coast Cape Coast Ghana

Illegal small scale gold mining popularly called “Galamsey” in our local communities is on the increase. This has led to concerns about the level of environmental pollution resulting from these mining activities. This work was conducted to determine the level of heavy metal contamination in the environment due to the activities of the small scale miners. This paper discusses the concentrations of some selected heavy metals—Hg, Pb, and Cu which were measured in 14 sampling sites in Dunkwa-on-Offin in the Central Region of Ghana, known for these activities. The heavy metal concentrations have been investigated for soil and water samples in the selected towns and compared with the relevant guidelines of the Environmental Protection Agency. The concentration of heavy metals was measured by using AAS. In most locations, the concentration for the investigated heavy metals far exceeded the concentration admitted by the guidelines. The mean concentration of Lead was 95.13 mg/Kg for soil and 190.27 mg/L in water;Copper was 63.26 mg/Kg in soil and 75.92 mg/L in water while Mercury was 140.87 ug/Kg in soil and 211.31 mg/L in water. The mean recorded concentrations in the sensitive areas exceeded greatly. Hence the levels of heavy metal contamination have spread beyond control.

关键词： Small-Scale Gold Mining Mercury Pollution Heavy Metal Dunkwa-on-Offin Ghana

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Evaluation and Management of Massive Endotracheal Hemorrhage in Pediatric Patients: A Case Report and Review of Literature

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International Journal of Otolaryngology and Head & Neck Surgery 2013年第6期2卷 236-239页

作者： Cory Vaughn Mona Shete Rose Mary Stocks jerome Thompson University of Tennessee Health Science Center Memphis USA

Massive hemoptysis is a frightening and life-threatening event in children. Prompt, aggressive evaluation and management are necessary. The most common cause of hemoptysis in a pediatric patient is infectious, but other various etiologies including tracheotomy related problems, cystic fibrosis, bronchiectasis, congenital anomalies of the cardiopulmonary vasculature and suction trauma must be considered as well. Presented is a report of a case of acute, massive endotracheal hemorrhage with multiple methods of management including balloon tamponade, highly selective embolization, and mainstem occlusion. This case is an addition to our previously reported case series of nine pediatric patients with massive pulmonary hemorrhage. Various diagnostic and management techniques of hemoptyis in pediatric patients are discussed with an extensive review of the literature.

关键词： Pediatric Massive Hemoptysis Endotracheal Tube Management

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The SHP-2 tyrosine phosphatase: Signaling mechanisms and biological functions

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Cell Research 2000年第4期10卷 279-288页

作者： QU CHENG KUI Department of Hematopoiesis, jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Department of Anatomy and Cell Biology, George Washington University School of Medicine and Health Sciences, Washington DC Department of Hematopoiesis American Red Cross Rockville MD 20855 USA. quc@***

Cellular biological activities are tightly controlled by intracellular signaling processes initiated by extracellular signals. Protein tyrosine phosphatases, which remove phosphate groups from phosphorylated signaling molecules, play equally important tyrosine roles as protein tyrosine kinases in signal transduction. SHP-2, a cytoplajsmic SH2 domain containing protein tyrosine phosphatase, is involved in the signaling pathways of a variety of growth factors and cytokines. Recent studies have clearly demonstrated that this phosphatase plays an important role in transducing signal relay from the cell surface to the nucleus, and is a critical intracellular regulator in mediating cell proliferation and differentiation.

关键词： SHP-2 SHP-1 Signal transduction.?

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Optical Properties of Iron to 30 eV

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Materials Sciences and Applications 2021年第12期12卷 622-638页

作者： jerome K. Butler Maria Martinez Reyhane Kilci Gary A. Evans Southern Methodist University Dallas USA Federal Center for Technological Education Celso Suckow da Fonseca CEFET/RJ Avenida Maracan? Rio de Janeiro Brazil

A modified approach closely related to the Drude, Lorentz-Drude and Brendel-Bormann theories is developed to fit the experimental data of the optical properties of metals. This work, while simplifying and redefining the parameters of previous models, can be directly compared with the parameters of the Brendel-Bormann model. As a test of validity, our model is compared with the Brendel-Bormann model and experimental data for gold. Our model shows excellent agreement with the experimental data for gold (up to 5 eV) and iron (up to 30 eV).

关键词： Isolators Waveguides Optical Properties Metals

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论处世（节选）

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英语角 2017年第8期 62-63页

作者： jerome Klapka jerome

喧嚣嘈杂，纷繁熙攘的人儿啊!王子与乞丐，罪人与圣者，屠夫、面包师和烛台制造商，补锅匠与裁缝，农家小伙与水手簇拥在一起。这里既有头戴假发、身着礼服的律师．又有头戴肮脏头饰的老年犹太裔旧衣商.这里既有身穿红色军装的士兵．又... 详细信息

喧嚣嘈杂，纷繁熙攘的人儿啊!王子与乞丐，罪人与圣者，屠夫、面包师和烛台制造商，补锅匠与裁缝，农家小伙与水手簇拥在一起。这里既有头戴假发、身着礼服的律师．又有头戴肮脏头饰的老年犹太裔旧衣商.这里既有身穿红色军装的士兵．又有戴着飘带帽子和旧棉手套，吹着哀乐的送葬者：这里既有迂腐顽固的学者．翻阅着早已发黄的书页．又有香气十足的演员，炫耀摆弄着自己的皮大衣：这里既有油嘴滑舌的政客．叫嚣着立法万能．又有走街串巷的小贩．高举着他那“包治百病”的狗皮膏药：这里既有圆滑老道的资本家．又有身强力壮的劳动者，这里既有科学家，又有擦鞋匠；这里既有诗人．又有收水费的人；这里既有内阁大臣，又有芭蕾舞演员；这里既有自卖自夸的糟鼻子酒商．又有每晚收取50英镑报酬的戒酒宣讲师：这里既有法官．又有骗子；这里既有神父．又有赌徒；这里既有满身珠光宝气、笑容可掬、雍容华贵的贵妇，又有早已厌烦了做饭，瘦骨嶙岣的客房服务员；还有嗲声嗲气、趾高气昂、浓妆艳抹的货色。

关键词：节选处世制造商劳动者资本家科学家芭蕾舞服务员

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Passive eye movements induced by electromagnetic force(EMF) in rats

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Zoological Research 2019年第3期40卷 211-218页

作者： Yue Yu Jun Huang Chun-Ming Zhang Tian-Wen Chen David S.Sandlin Shao-Xun Wang Alberto A.Arteaga jerome Allison Yang Ou Susan Warren Paul May Hong Zhu Wu Zhou Departments of Otolaryngology and Communicative Sciences University of Mississippi Medical CenterJackson MS 39216USA Department of Otolaryngology First Affiliated HospitalShanxi Medical UniversityTaiyuan Shanxi 030001China MD/PhD Program University of Mississippi Medical CenterJackson MS 39216USA Pharmacology and Toxicology University of Mississippi Medical CenterJackson MS 39216.USA Neurobiology and Anatomical Sciences University of Mississippi Medical CenterJackson MS 39216USA Neurology University of Mississippi Medical CenterJackson MS 39216USA

Accurate information on eye position in the orbit is available from visual feedback,efference copy of the oculomotor commands and proprioceptive signals from the extraocular muscles (EOM).Whereas visual feedback and oculomotor commands have been extensively studied,central processing of EOM proprioceptive signals remains to be elucidated.A challenge to the field is to develop an approach to induce passive eye movements without physically contacting the eyes.A novel method was developed to generate passive eye movements in rats.A small rare-earth magnet disk (0.7 mm diameter,0.5 mm thickness) was attached to the surface of a rat's eyeball.A metal rod (5 mm diameter) wrapped with an electromagnetic (EM) coil was placed near the magnet (8-15 mm).By passing currents to the EM coil,electromagnetic force (EMF) was generated and acted upon the magnet and induced passive eye *** EMF induced well-defined passive eye movements,whose directions were dependent on current polarity and amplitudes and peak velocities were dependent on current intensity and *** velocities of the EMF-induced eye movements were linearly related to amplitudes,exhibiting main sequence relationships similar to that of saccades in awake rats and eye movements induced by electrical microstimulation of the abducens nucleus in anesthetized *** examination showed that repetitive EMF stimulations did not appear to result in damages in the EOM *** results validated the EMF approach as a novel tool to investigate EOM proprioceptive signals and their roles in visual localization and gaze control.

关键词： Eye movement Proprioception Extraocular muscles Stretch reflex

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