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Development of soft kernel durum wheat

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frontiers of Agricultural Science and Engineering 2019年第3期6卷 273-278页

作者： Craig f.morris USDA-ARS Western Wheat Quality Laboratory Washington State University

Kernel texture(grain hardness) is a fundamental and determining factor related to wheat(Triticum spp.) milling, baking and flour *** are three kernel texture classes in wheat: soft and hard hexaploid(***), and very hard durum(*** ***).The genetic basis for these three classes lies with the Puroindoline ***, the easiest means of quantifying kernel texture is with the Single Kernel Characterization System(SKCS), although other means are valid and can provide fundamental material *** SKCS values for soft wheat would be around 25 and for durum wheat≥*** kernel durum wheat was created via homeologous recombination using the ph1b mutation, which facilitated the transfer of ca.28 Mbp of5 DS that replaced ca.21 Mbp of 5 *** 5 DS translocation contained a complete and intact Hardness locus and both Puroindoline *** of the Puroindoline genes in durum grain resulted in kernel texture and flour milling characteristics nearly identical to that of soft wheat, with high yields of break and straightgrade flours, which had small particle size and low starch *** water absorption was markedly reduced compared to durum flour and *** strength was essentially unchanged and reflected the inherent gluten properties of the durum *** quality was essentially equal-to-or-better than pasta made from ***, soft durum germplasm showed good potential with moderate grain yield and resistance to a number of fungal pathogens and *** breeding efforts will no doubt further improve the quality and competitiveness of soft durum cultivars.

关键词： soft durum wheat grain hardness Puroindolines milling baking pasta noodles

Arabidopsis ADC1 functions as an Nδ-acetylornithine decarboxylase

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Journal of Integrative Plant Biology 2020年第5期62卷 601-613页

作者： Yann-Ru Lou Sheaza Ahmed Jian Yan Adewale M.Adio Hannah M.Powell Paul f.morris Georg Jander Boyce Thompson Institute for Plant Research IthacaNY 14853USA Department of Biological Sciences Bowling Green State UniversityBowling GreenOH 43403USA Key Laboratory of Agro-Environment in the Tropics Ministry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou 510642China Department of Biochemistry and Molecular Biology Michigan State UniversityEast LansingMI 48824USA Bayer Crop Science 700 Chesterfield Parkway WestChesterfieldMO 63017USA

Polyamines are small aliphatic amines found in almost all organisms,ranging from bacteria to plants and *** most plants,putrescine,the metabolic precursor for longer polyamines,such as spermidine and spermine,is produced from arginine,with either agmatine or ornithine as *** we show that Arabidopsis thaliana(Arabidopsis)arginine de-carboxylase 1(ADC1),one of the two known arginine decarboxylases in Arabidopsis,not only synthesizes agmatine from arginine,but also converts *** to *** analyses indicate that duplication and neofunctionalization of ADC1 and NATA1,the enzymes that synthesize Nδ-acetylormithine in Arabidopsis,co-occur in a small number of related species in the *** ADC2,which is localized in the chloroplasts,ADC1 is in the endoplasmic reticulum together with NATA1,an indication that these two enzymes have access to the same substrate ***,these results are con-sistent with a model whereby NATA1 and ADC1 to-gether provide a pathway for the synthesis of N-acetylputrescine in Arabidopsis.

关键词： Arabidopsis Phylogenetic Together

硬质小麦籽粒阿拉伯木聚糖含量的遗传变异研究

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麦类作物学报 2007年第6期27卷 1016-1022页

作者：李硕碧 Craig f.morris Arthur D.Bettge 西北农林科技大学陕西杨凌712100 美国农业部西部小麦品质实验室

为了更好地理解环境和基因型对小麦阿拉伯木聚糖含量的影响,为小麦品质改良提供科学依据,采用比色法测定了美国西部51个硬质小麦品种的阿拉伯木聚糖组分含量,这些小麦品种包括冬小麦和春小麦,分别被种植在3个不同的地点。结果表明,参试... 详细信息

为了更好地理解环境和基因型对小麦阿拉伯木聚糖含量的影响,为小麦品质改良提供科学依据,采用比色法测定了美国西部51个硬质小麦品种的阿拉伯木聚糖组分含量,这些小麦品种包括冬小麦和春小麦,分别被种植在3个不同的地点。结果表明,参试冬小麦品种水溶性和总阿拉伯木聚糖含量的变异范围分别为0.39%-0.81%和3.09%-4.04%,春小麦品种这两种木聚糖含量范围分别为0.48%-0.92%和3.94%-4.70%。小麦籽粒阿拉伯木聚糖含量的变异同时受品种、环境及其互作效应的显著影响,环境效应大于品种效应。小麦品种间阿拉伯木聚糖含量的变异主要表现在水溶性组分上。就不同组分而言,基因型对水溶性阿拉伯木聚糖组分的影响相对较大,环境则分别对冬小麦水不溶性、春小麦水溶性阿拉伯木聚糖组分有较大的影响。品种间和环境间阿拉伯木聚糖含量的变异,冬小麦大于春小麦;水溶性阿拉伯木聚糖含量的遗传型方差春小麦大于冬小麦。冬、春小麦的水溶性阿拉伯木聚糖含量的广义遗传力均大于95%,通过遗传育种的方法改良小麦阿拉伯木聚糖含量是可行的。冬小麦与春小麦阿拉伯木聚糖含量的平均值之间无明显差异。

关键词：小麦环境因素阿拉伯木聚糖变异

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皮肤穿支血管的解剖学研究

中国临床解剖学杂志 2006年第3期24卷 232-235页

作者：杨大平唐茂林 Christopher R.Geddes Steve f.morris 哈尔滨医科大学附属第二医院哈尔滨150086 温州医学院解剖学教研室浙江温州325035 Division of Plastic Surgery Dalhousie UniversityHalifaxB3H 3A7Canada

目的:对全身皮肤血管区域进行定性和定量分析,确定全身皮肤穿支血管的位置、数量、口径、穿支蒂的长度、类型、来源血管以及穿支所供应皮肤的面积,为穿支皮瓣提供血管解剖基础。方法:选用10具新鲜尸体,采用改良氧化铅-明胶灌注技术进行... 详细信息

目的:对全身皮肤血管区域进行定性和定量分析,确定全身皮肤穿支血管的位置、数量、口径、穿支蒂的长度、类型、来源血管以及穿支所供应皮肤的面积,为穿支皮瓣提供血管解剖基础。方法:选用10具新鲜尸体,采用改良氧化铅-明胶灌注技术进行动脉灌注。将每个口径大于0.5mm的穿支血管进行解剖并记录。拍摄X线片以显示皮肤内血管的形态和分布。定量数据分析包括全身各部位的穿支血管的数量、口径、类型及其供应区域的面积。结果:发现全身128支起源血管发出440支穿支供应皮肤。其中肌皮穿支与肌间隔穿支之比为3:2。穿支平均直径为0.7mm。穿支血管的分布规律如下:①躯干皮肤的血供主要来自肌皮穿支,这些穿支在皮肤内的走行距离和分布范围大于肢体皮肤的穿支。②肢体皮肤的血供主要来自肌间隙穿支血管,主要分布在深筋膜表面,皮神经和浅静脉周围穿支之间形成链式血管吻合。③单位面积的穿支数量与皮肤的移动程度成反比,穿支的口径大小和穿支在皮肤内走行距离与皮肤移动度成正比,与穿支的供应面积成正比。结论:本研究提供详细的皮肤穿支血管解剖数据,为临床设计应用穿支皮瓣提供解剖学依据。

关键词：穿支血管穿支皮瓣血管区域新鲜尸体定量分析

Exploring novel candidate genes from the Mouse Genome Informatics database: Potential implications for avian migration research

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Integrative Zoology 2016年第4期11卷 240-249页

作者： Andrea CONTINA Eli S.BRIDGE jeffrey f.KELLY Oklahoma Biological Survey University of OklahomaNormanOklahomaUSA Department of Biology University of OklahomaNormanOklahomaUSA

To search for genes associated with migratory phenotypes in songbirds,we selected candidate genes through annotations from the Mouse Genome Informatics database and assembled an extensive candidate-gene ***,we implemented a next-generation sequencing approach to obtain DNA sequences from the Painted Bunting *** focused on those sequences that were conserved across avian species and that aligned with candidate genes in our mouse *** genotyped short sequence repeats from the following candidate genes:ADRA1d,ANKRD17,CISH and *** studied the possible correlations between allelic variations occurring in these novel candidate migration genes and avian migratory phenotypes available from the published lit*** found that allele variation at MYH7 correlated with a calculated index of speed of migration(km/day)across 11 species of *** highlight the potential of the Mouse Genome Informatics database in providing new candidate genes that might play a crucial role in regulating migration in birds and possibly in other *** research effort shows the benefits and limitations of working with extensive genomic datasets and offers a snapshot of the challenges related to cross-species validation in behavioral and molecular ecology studies.

关键词： avian migration candidate gene approach Mouse Genome Informatics MYH7 songbirds

Comments by opponents on the British Medical Association’s guidance on non-therapeutic male circumcision of children seem one-sided and may undermine public health

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World Journal of Clinical Pediatrics 2023年第5期12卷 244-262页

作者： Stephen Moreton Guy Cox Mark Sheldon Stefan A Bailis jeffrey D Klausner Brian J morris Circfacts Warrington WA51HYCheshireUnited Kingdom Australian Centre for Microscopy&Microanalysis and School of Aeronautical Mechanical and Mechatronic EngineeringUniversity of SydneySydney 2006New South WalesAustralia Medical Humanities and Bioethics Program Feinberg School of MedicineNorthwestern UniversityChicagoIL 60661United States Cornerstone Therapy&Recovery Center St.PaulMN 55101United States Department of Medicine Population and Public Health SciencesKeck School of MedicineUniversity of Southern CaliforniaLos AngelesCA 90089United States School of Medical Sciences University of SydneySydney 2006New South WalesAustralia

The British Medical Association(BMA)guidance on non-therapeutic circumcision(NTMC)of male children is limited to ethical,legal and religious *** we evaluate criticisms of the BMA’s guidance by Lempert et *** their arguments promoting autonomy and consent might be superficially appealing,their claim of high procedural risks and negligible benefits seem one-sided and contrast with high quality evidence of low risk and lifelong *** literature reviews by the American Academy of Pediatrics and the United States Centers for Disease Control and Prevention in developing evidence-based policies,as well as risk-benefit analyses,have found that the medical benefits of infant NTMC greatly exceed the risks,and there is no reduction in sexual function and *** BMA’s failure to consider the medical benefits of early childhood NTMC may partly explain why this prophylactic intervention is discouraged in the United *** consequence is higher prevalence of preventable infections,adverse medical conditions,suffering and net costs to the UK’s National Health Service for treatment of *** of the issues and contradictions in the BMA guidance identified by Lempert et al stem from the BMA’s guidance not being sufficiently ***,that document called for a review by others of the medical issues surrounding *** societal factors apply,ultimately,NTMC can only be justified rationally on scientific,evidence-based *** are entitled to an accurate presentation of the medical evidence so that they can make an informed *** decision either for or against NTMC should then be respected.

关键词： Circumcision,Male Child Infections Risk Policy Public health

Interfacial Charge Transfer in Nanoscale Polymer Transistors

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Nano Research 2008年第4期1卷 341-350页

作者： jeffrey H.Worne Rajiv Giridharagopal Kevin f.Kelly Douglas Natelson Department of Electrical and Computer Engineering Rice University6100 Main St.HoustonTX 77005USA Department of Physics and Astronomy Rice University6100 Main St.HoustonTX 77005USA

Interfacial charge transfer plays an essential role in establishing the relative alignment of the metal fermi level and the energy bands of organic *** the details remain elusive in many systems,this charge transfer has been inferred in a number of photoemission *** present electronic transport measurements in very short channel(Lfrom poly(3-hexylthiophene)(P3HT).As channel length is reduced,the evolution of the contact resistance and the zero gate voltage conductance are consistent with such charge *** channel conduction in devices with Pt contacts is greatly enhanced compared to analogous devices with Au contacts,consistent with charge transfer *** current scanning tunneling microscopy(ACSTM)provides further evidence that holes are transferred from Pt into P3HT,while much less charge transfer takes place at the Au/P3HT interface.

关键词： Organic semiconductors band alignment charge transfer organic field-effect transistor scanning tunneling microscopy

A conserved oomycete effector RxLR23 triggers plant defense responses by targeting ERD15La to release NbNAC68

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A conserved oomycete effector RxLR23 triggers plant defense ...

Cold-inducible RNA-binding protein activates splenic T cells during sepsis in a TLR4-dependent manner

中国菌物学会2023年学术年会

作者： Hui Sheng Congcong Ai Cancan Yang Chunyuan Zhu Zhe Meng fengzhi Wu Xiaodan Wang Daolong Dou Paul f.morris Xiuguo Zhang Shandong Agricultural University College of Plant ProtectionShandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests Shandong Agricultural University College of Life SciencesState Key Laboratory of Crop Biology Shandong Normal University College of Life Sciences Northeast Agricultural University Department of Horticulture China Agricultural University College of Plant Protection Nanjing Agricultural University Department of Plant Pathology Bowling Green State University Department of Biological Sciences

Oomycete pathogens deliver many effectors to enhance virulence or suppress plant *** immune networks are interconnected,in which a few effectors can trigger a strong defense response when recognized by immunity-related *** effectors activate plant defense response remains poorly *** we report Phytophthora capsici effector RxLR23 can induce plant cell death and plant ***23 specifically binds to NbERD15La,a regulator of abscisic acid and salicylic acid pathway,and the binding intensity depends on the amino acid residues(K93and M320).NbNAC68,a downstream protein of ERD15La,can stimulate plant immunity that is compromised after binding with *** of NbNAC68substantially prevents the activation of plant defense ***23 binds to ERD15La,releasing NbNAC68 to activate plant immunity These findings highlight the importance of RxLR23activity as a strategy of plant defense responses.

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Cellular & Molecular Immunology 2018年第1期15卷 38-47页

作者： Alexandra C Bolognese Archna Sharma Weng-Lang Yang jeffrey Nicastro Gene f Coppa Ping Wang Elmezzi Graduate School of Molecular Medicine Manhasset11030NYUSA Department of Surgery Hofstra Northwell School of MedicineManhasset11030NYUSA Center for Immunology and Inflammation The Feinstein Institute for Medical ResearchManhasset11030NYUSA

Cold-inducible RNA-binding protein (CIRP) is a novel inflammatory mediator that stimulates the release of proinflammatory cytokines from macrophages in sepsis. Given the immune dysregulation that characterizes sepsis, the effect of CIRP on other immune cells is an area of increasing interest that has not yet been studied. In the present study, we hypothesized that extracellular CIRP promotes activation of T lymphocytes in the spleen during sepsis. We observed that mice subjected to sepsis by cecal ligation and puncture showed significantly higher expression of the early activation markers CD69 and CD25 at 20 h on CD4+ splenic T cells, and significantly higher CD69 expression on CD8+ splenic T cells compared with sham-operated controls. furthermore, at 20 h after receiving intravenous injection of recombinant murine CIRP (rmCIRP, 5 mg/kg body weight (BW)) or PBS (vehicle), those mice receiving rmCIRP showed significantly increased expression of CD69 and CD25 on both CD4+ and CD8+ splenic T cells. This effect, however, was not seen in TLR4-deficient mice after rmCIRP injection. In addition, treatment with CIRP predisposed CD4+ T cells to a Th1 hyperinflammatory response profile, and influenced CD8+ T cells toward a cytotoxic profile. Taken together, our findings indicate that CIRP is a proinflammatory mediator that plays an important role in T-cell dysregulation during sepsis in a TLR4-dependent manner.

关键词： CIRP immune response lymphocytes sepsis T-cell activation