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Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

作物学报:英文版 2023年第5期11卷 1512-1520页

作者： Caiyun Liu Wei Guo Yang Wang Bisheng Fu jaroslav doležel Ying Liu Wenling Zhai Mahmoud Said István Molnár Kateřina Holušová Ruiqi Zhang Jizhong Wu Institute of Germplasm Resources and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina College of Agronomy/JCIC-MCP/National Key Lab of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Experimental Botany Centre of the HanáRegion for Biotechnological and Agricultural ResearchŠlechtitelů31CZ-783671 Olomoucthe Czech Republic Field Crops Research Institute Agricultural Research Centre9 Gamma StreetGiz112619 CairoEgypt Agricultural Institute Centre for Agricultural ResearchELKHMartonvásár 2462Hungary Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Zhongshan Biological Breeding Laboratory Nanjing 210014JiangsuChina

Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.

关键词： Triticum aestivum Rhizoctonia cerealis Dasypyrum villosum Flow sorting KASP

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Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa,a wild relative of common wheat

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The Crop Journal 2020年第4期8卷 676-681页

作者： Jia Lei Jiawen Zhou Haojie Sun Wentao Wan Jin Xiao Chunxia Yuan Miroslava Karafiátová jaroslav dolezel Haiyan Wang Xiue Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjing 210095JiangsuChina Institute of Experimental Botany Czech Academy of SciencesCentre of the Region Hana for Biotechnological and Agricultural ResearchŠlechtitelů3177900 OlomoucCzech Republic

Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat *** to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of *** can be characterized is poor,hampering accurate characterization of small segmental alien *** designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of *** chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on *** *** these,we constructed FISH karyotypes for *** using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different *** *** new FISH probes can efficiently characterize *** introgressions into wheat.

关键词： Chromosome identification Haynaldia villosa Oligonucleotide probes Tandem DNA repeats

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Sequence analysis of Erianthus arundinaceus chromosome 1 isolated by flow sorting after genomic in situ hybridization in suspension

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The Crop Journal 2022年第6期10卷 1746-1754页

作者： Shan Yang Petr Cápal jaroslav dolezel Xueting Li Wang Qian Zhiqiang Wang Kai Zeng Peiting Li Hongkai Zhou Rui Xia Muqing Zhang Zuhu Deng National Engineering Research Center for Sugarcane&State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources&Guangxi Key Laboratory of Sugarcane Biology Guangxi UniversityNanning 530004GuangxiChina Institute of Experimental Botany of the Czech Academy of Sciences Center of the Region Hanáfor Biotechnological and Agricultural ResearchOlomouc CZ-77900Czech Republic College of Coastal Agricultural Sciences Guangdong Ocean UniversityZhanjiang 524088GuangdongChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642GuangdongChina

Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic ***,limited knowledge of the complex genome of hexaploid *** has impeded the development of required molecular *** complex genomes into single chromosomes can simplify *** flow-cytometric sorting of a single chromosome of *** in a Saccharum-Erianthus introgression line is reported.A novel approach called genomic in situ hybridization in suspension was used to discriminate the alien chromosome from sugarcane chromosomes at the same size.A total of 218,000 *** chromosome 1(EaC1)were sorted to>97%purity and amplified DNA was sequenced using Illumina and Pac Bio *** resulting assembly had a 70.93 Mb contig sequence with an N50 of 19.62 kb.A total of 56.69 Mb repeat sequences were predicted,accounting for 79.1%of the chromosome and 2646 genes having a total length of 1.84 Mb that represented 2.59%of the *** these genes,1877(70.9%)genes were functionally *** phylogenetic relationship of *** with other species using the chromosome1 sequence revealed that *** was distantly related to Oryza sativa and Zea mays,followed by Sorghum bicolor,and was closely related to *** and Saccharum *** study provides the first insights into the characteristics of EaC1,and the results will provide tools to support molecular improvement and alien introgression breeding of sugarcane.

关键词： Flow cytometry GISHIS Chromosome sorting Genome analysis Sugarcane

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Social networks of reintroduced Przewalski's horses in the Great Gobi B Strictly Protected Area (Mongolia)

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Current Zoology 2024年第2期70卷 182-194页

作者： Anna Bernatkova Ganbaatar Oyunsaikhan jaroslav Simek Martina Komarkova Francisco Ceacero Faculty of Tropical AgriSciences Czech University of Life Sciences PragueCzech Republic Great Gobi B Strictly Protected Area Takhin TalMongolia Zoo Praha U Trojského zamku 120/3PragueCzech Republic

Analyzing social networks is a powerful tool for understanding the ecology of social *** most studies focus on the role of each group member,few compare groups with different *** current population of Przewalski's horses Equus ferus przewalskii at the Great Gobi B Strictly Protected Area(Mongolia)includes groups of wild-born and captive-bred individuals with different experiences with the area(acclimatizing,long-term reintroduced,and wild-born),therefore serving as an ideal natural behavioral *** filmed 11 groups for 141.5 hours in summer 2018(July),late spring 2019(May,June),and autumn 2019(September,October).Affiliative and agonistic interactions were recorded,and social networks were *** tested the influence of origin,experience,season,sex,age,relative time belonging to the group,relat-edness,and dominance rank on different network indices at the individual and group *** found that groups with greater experience in the area are generally better connected than members of the newly formed ***,these strong networks were created by wild-born individuals with very low interaction *** the contrary,inexperienced groups composed of captive-bred individuals displayed many inter actions but created weak social *** results show a trend of behavioral transition from acclimatizing through long-term reintroduced to wild-born groups,supporting that the newly formed groups of released Przewalski's horses need time to display the typical social behavior patterns of wild-born ***-term monitoring of released Przewalski's horses is recommended to promote the success of this reintroduction program.

关键词： Przewalski's horses reintroduction social behavior soft-release.

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基于染色体分拣及二代测序技术内含子靶向的外源染色体特异分子标记的开发

基于染色体分拣及二代测序技术内含子靶向的外源染色体特异分子标...

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江苏省遗传学会2016年学术年会

作者：王海燕代渴丽肖进袁春霞赵仁慧 jaroslav dole?el 吴玉峰曹爱忠陈佩度张守忠王秀娥南京农业大学细胞遗传研究所作物遗传与种质创新国家重点实验室

簇毛麦（Haynaldiavillosa,VV,2n=14）是普通小麦的二倍体近缘物种,是小麦遗传改良的重要基因资源。实验室前期研究中,发现簇毛麦4V染色体短臂（4VS）上携带有抗小麦黄花叶病、抗全蚀病等优异基因,并通过ph1b突变体诱导部分同源染色体... 详细信息

簇毛麦（Haynaldiavillosa,VV,2n=14）是普通小麦的二倍体近缘物种,是小麦遗传改良的重要基因资源。实验室前期研究中,发现簇毛麦4V染色体短臂（4VS）上携带有抗小麦黄花叶病、抗全蚀病等优异基因,并通过ph1b突变体诱导部分同源染色体配对和花粉辐射创制了涉及4V染色体的一系列结构变异体,对这些结构变异体的快速和精确鉴定将有助于外源基因的准确定位,覆盖全染色体、分布均匀的高密度外源染色体特异分子标记开发是外源染色体片段准确鉴定的基础。本研究在实验室选育了4VS染色体端体异附加系的基础上,利用流式细胞仪分拣出4VS染色体臂,利用高通量测序获得和拼接了4VS基因组组装序列。将拼接序列与已经公布的中国春4AL,4BS,4DS以及粗山羊草的4DS序列进行比对,发现存在大量的直系同源基因,证明它们之间良好的共线性关系。为开发4VS特异的高密度分子标记,筛选出包含内含子的直系同源基因,比较4VS和4AL,4BS和4DS同源基因内含子大小,选择4VS内含子大小分别都与中国春4AL,4BS,4DS对应内含子差异10%以上的序列,在簇毛麦该内含子前后相临近的两个外显子序列上分别设计PCR引物,开发跨内含子的分子标记,即内含子靶向的分子标记（Intron Targeted Marker,ITmarker）。本研究共设计359对引物,在中国春小麦（AABBDD）、簇毛麦（VV）、硬粒小麦-簇毛麦双二倍体（AABBVV）和小麦-簇毛麦易位系（T4DL·4VS）中扩增表明,232个为4VS特异的分子标记,多态率达64.62%,显著高于传统的外源染色体特异分子标记开发方法。

关键词：小麦簇毛麦分子标记内含子多态性

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Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat

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The Crop Journal 2020年第4期

作者： Jia Lei Jiawen Zhou Haojie Sun Wentao Wan Jin Xiao Chunxia Yuan Miroslava Karafiátová jaroslav doležel Haiyan Wang Xiue Wang

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Enigmatic thymus: Variations in anatomical localisation of thymic tissue as an easily misdiagnosed congenital anomaly in surgical practice

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World Journal of Clinical Cases 2024年第25期12卷 5646-5652页

作者： Alexander Mayer jaroslav Voller Ivan Varga Fourth Department of Surgery Faculty of MedicineComenius University and University Hospital BratislavaBratislava 81372Slovakia Faculty of Health Care Studies University of Western BohemiaPilsen 30100Czech Republic Institute of Histology and Embryology Faculty of MedicineComenius University in BratislavaBratislava 81372Slovakia

We point out the issue of differential diagnosis regarding the finding of ectopically localised thymic tissue(a thymic cyst)in the *** tissue can be found anywhere along its developmental tract of descent,from the angle of the mandible to the upper *** of the thymic descent can result in ectopically/abnormally localised islets of accessory thymic tissue,which may undergo cystic changes,as described in a case report by Sun et *** anatomical variation of the thymus may be clinically misinterpreted as a neoplasm or other congenital anomalies as a branchial cyst,lymphatic malformation or cystic *** present editorial focuses on the challenge of establishing a diagnosis of ectopically localised tissue of thymus often presented as a lateral cervical mass,especially in the case of cystic variation/degeneration of this thymic *** summarise hypotheses on the origin of such congenital cervical thymic cysts from the point of view of evolutionary history and *** also discuss lesser-known facts about the anatomy,histopathology and developmental biology of the thymus as one of the most enigmatic organs in the human body.

关键词： Thymus Thymic anomalies Thymic ectopy Congenital cervical thymic cyst Embryonic development

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Antimicrobial susceptibility of Enterobacteriaceae causing urinary tract infections： a central military hospital study

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Chinese Medical Journal 2014年第23期127卷 4150-4151页

作者： jaroslav Timko Faculty of Health Care Catholic University in Ruzomberok Institute of Clinical Microbiology of Central Military Hospital - Faculty Hospital Ruzomberok Slovakia

Urinary tract infection （UTI） remains one of the most common bacterial infections seen in hospitalized patients of all ages. Prevalence of UTIs increases with age and is often accompanied by higher resistance rates. The aim of this study was to determine the degree of resistance of most common enterobacterial uropathogens isolated from patients at Central Military Hospital and compare this resistance with the results found by the North America and Europe Study for Monitoring Antimicrobial Resistance Trends （SMART） study during 2009-2010.

关键词： urinary tract infection uropathogens Escherichia coli resistance ampicillin-sulbactam

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Evaluation of granulability and absorbtivity of biomass in agglomeration mixture

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Journal of Central South University 2017年第10期24卷 2260-2265页

作者： Hudák Jozef Lesko jaroslav Semanová Zuzana Frohlichová Mária Findorák Róbert Legemza jaroslav Institute of Metallurgy Faculty of MetallurgyTechnical University of KosiceKosice04200Slovakia

The granulability and absorbtivity of sawdust and coke breeze were *** properties are important within the preparation of agglomeration *** have a significant impact on plynodynamic batch *** of materials to wet is a fundamental condition of granulability of *** and water absorption of wood with liquids is generally significantly affected by their chemical *** important factors affecting the wetting process are undoubtedly the wood moisture content,the value of the surface tension of liquid media and their chemical *** increasing moisture content of wood,the time to equilibrium *** of water absorption and granulability of sawdust was conducted to seek their possible use as a partial replacement of coke dust in the production of the *** have been realized with alder,oak and pine sawdust and reference material-coke *** materials were divided to four classes of granularity:supplied material,1-2 mm,0.5-1 mm and less than 0.5 *** different liquid media were used:water 16°C,water 60°C,soapy water,lime milk and surfactant.

关键词： agglomeration biomass coke breeze granulability absorbtivity

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Classification of Spectra of Emission Line Stars Using Machine Learning Techniques

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International Journal of Automation and computing 2014年第3期11卷 265-273页

作者： Pavla Bromová Petr koda jaroslav Vázn Faculty of Information Technology Brno University of Technology Astronomical Institute of the Academy of Sciences of the Czech Republic

Advances in the technology of astronomical spectra acquisition have resulted in an enormous amount of data available in world-wide telescope archives. It is no longer feasible to analyze them using classical approaches, so a new astronomical discipline,astroinformatics, has emerged. We describe the initial experiments in the investigation of spectral line profiles of emission line stars using machine learning with attempt to automatically identify Be and B[e] stars spectra in large archives and classify their types in an automatic manner. Due to the size of spectra collections, the dimension reduction techniques based on wavelet transformation are studied as well. The result clearly justifies that machine learning is able to distinguish different shapes of line profiles even after drastic dimension reduction.

关键词： Be star stellar spectrum feature extraction dimension reduction discrete wavelet transform classification support vector machines(SVM) clustering.

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