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Localised solid-state nanopore fabrication via controlled breakdown using on-chip electrodes

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Nano Research 2022年第11期15卷 9881-9889页

作者： Jasper P.Fried Jacob L.Swett Binoy Paulose Nadappuram Aleksandra Fedosyuk Alex Gee Ondrej E.Dyck James R.Yates Aleksandar P.Ivanov Joshua B.Edel jan a.mol Department of Materials University of OxfordOxfordOX13PHUK School of Chemistry University of New South WalesSydneyNew South Wales 2052Australia Department of Chemistry Imperial College LondonLondonW120BZUK Center for Nanophase Materials Sciences Oak Ridge National LaboratoryOak RidgeTN 37830USA Instituto de Tecnologia Química e Biológica António Xavier Universidade Nova de LisboaAv.da RepúblicaOeiras 2780-157Portugal School of Physics and Astronomy Queen Mary University of LondonLondonE14NSUK

Controlled breakdown has recently emerged as a highly accessible technique to fabricate solid-state ***,in its most common form,controlled breakdown creates a single nanopore at an arbitrary location in the ***,we introduce a new strategy whereby breakdown is performed by applying the electric field between an on-chip electrode and an electrolyte solution in contact with the opposite side of the *** demonstrate two advantages of this ***,we can independently fabricate multiple nanopores at given positions in the membrane by localising the applied field to the ***,we can create nanopores that are self-aligned with complementary nanoelectrodes by applying voltages to the on-chip electrodes to locally heat the membrane during controlled *** new controlled breakdown method provides a path towards the affordable,rapid,and automatable fabrication of arrays of nanopores self-aligned with complementary on-chip nanostructures.

关键词： solid-state nanopores dielectric breakdown nanofabrication single-molecule sensing nanopore arrays

Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

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Insect Science 2019年第4期26卷 656-670页

作者： Arkadiusz Urbanski jan Lubawy Pawel Marciniak Grzegorz Rosinski Faculty of Biology Department of Animal Physiology and Development Adam Mickiewicz University in Poznan Poznan Poland

Burying beetles (Nicrophorus sp.) are necrophagous insects with developed parental care. Genome of Nicrophorus vespilloides has been recently sequenced, which makes them interesting model organism in behavioral ecology. However, we know very little about their physiology, including the functioning of their neuroendocrine system. In this study, one of the physiological activities of proctolin, myosuppressin (Nieve? MS), myoinhibitory peptide (Trica-MIP-5) and the short neuropeptide F (Nicve-sNPF) in N. vespilloides have been investigated. The tested neuropeptides were myoactive on N. vespilloides hindgut. After application of the proctolin increased hindgut contraction frequency was observed (EC50 value was 5.47 x 10-8 mol/L). The other tested neuropeptides led to inhibition of N. vespilloides hindgut contractions (Nicve-MS: IC50 = 5.20 x 10~5 mol/L;Trica-MIP-5: IC50 = 5.95 x 10-6 mol/L;Nicvc-sNPF: IC50 = 4.08 x 10-5 mol/L). Moreover, the tested neuropeptides were immunolocalized in the nervous system of N. vespilloides. Neurons containing sNPF and MIP in brain and ventral nerve cord (VNC) were identified. Proctolin-immunolabeled neurons only in VNC were observed. Moreover, MIP-immunolabeled varicosities and fibers in retrocerebral complex were observed. In addition, our results have been supplemented with alignments of amino acid sequences of these neuropeptides in beetle species. This alignment analysis clearly showed amino acid sequence similarities between neuropeptides. Moreover, this allowed to deduce amino acid sequence of N. vespilloides proctolin (RYLPTa), Nicve-MS (QDVDHVFLRFa) and six isoforms ofNicve-MIP (Nicve-MIP-1一 DWNRNLHSWa;Nicve-MIP-2—AWQNLQGGWa;Nicve-MIP-3—AWQNLQGGWa;Nicve-MlP-4—AWKNLNNAGWa;Nicve-MIP-5—SEWGNFRGSWa;Nicve-MIP-6— DPAWTNLKGIWa;and Nicve-sNPF—SGRSPSLRLRFa).

关键词： burying beetles immunolocalization myotropic activity neuropeptides nervous system

Is Chloroplast Movement in Tobacco Plants Influenced Systemically after Local Illumination or Burning Stress?

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Journal of Integrative Plant Biology 2008年第10期50卷 1292-1299页

作者： jan Nau Monika Rolencov Vladimíra Hlavkov Laboratory of Biophysics Department of Experimental Physics Palacky' University tr. Svobody 26 77146 Olomouc Czech Republic

Chloroplast movement has been studied in many plants mainly in relation to the local light, mechanical or stress effects. Here we investigated possible systemic responses of chloroplast movement to local light or burning stress in tobacco plants （Nicotisna tabacum cv. Samsun）. Chloroplast movement was measured using two independent methods： one with a SPAD 502 Chlorophyll meter and another by collimated transmittance at a selected wavelength （676 nm）. A sensitive periodic movement of chloroplasts was used in high or low （2 000 or 50 μmol/m2 per s photosynthetically active radiation, respectively） cold white light with periods of 50 or 130 min. Measurements were carried out in the irradiated area, in the non-irradisted area of the same leaf or in the leaf located on the stem below the irradiated or bumed one. No significant changes in systemic chloroplast movement in non-irradiated parts of the leaf and in the non-treated leaf were detected. Our data indicate that chloroplast movement in tobacco is dependent dominantly on the intensity and spectral composition of the incident light and on the local stimulation and state of the target tissue. No systemic signal was strong enough to evoke a detectable systemic response in chloroplast movement in distant untreated tissues of tobacco plants.

关键词： buming chloroplast movement irradiance systemic response tobacco.

Recent progress in the structure optimization and development of proton-conducting electrolyte materials for low-temperature solid oxide cells

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International Journal of Minerals,Metallurgy and Materials 2022年第4期29卷 848-869页

作者： Jia Song Yuvraj Y.Birdja Deepak Pant Zhiyuan Chen jan Vaes Separation and Conversion Technology Flemish Institute for Technological Research(VITO)Mol 2400Belgium

This work reviews technologies that can be used to develop low-temperature solid oxide cells(LT-SOCs)and can be applied in fuel cells and electrolyzers operating at temperatures below 500℃,thus providing a more cost-effective alternative than conventional high-temperature *** routes showing potential to reduce the operating temperature of SOCs to below 500℃ are *** first route is the principal way to enhance cell performance,namely,structure *** technique includes the reduction of electrolyte thickness to the nanometer scale and the exploration of electrode structure with low polarization *** other route is the development of novel protonconducting electrolyte materials,which is in the frontier of SOCs *** fundamentals of proton conduction and the design principles of commonly used electrolyte materials are briefly *** most widely studied electrolyte materials for LT-SOCs,namely,perovskitetype BaCeO_(3) -and BaZrO_(3) -based oxides,and the effect of doping on the physical-chemical properties of these oxide materials are summarized.

关键词： low-temperature solid oxide cells perovskite doping thin-film

Indole phytoalexin derivatives induce mitochondrialmediated apoptosis in human colorectal carcinoma cells

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World Journal of Gastroenterology 2017年第24期23卷 4341-4353页

作者： Viera Tischlerova Martin Kello Mariana Budovska jan Mojzis Department of Pharmacology Faculty of MedicinePavol Jozef Safarik University Department of Organic Chemistry Institute of ChemistryFaculty of SciencePavol Jozef Safarik University

To investigate the mechanism of the antiproliferative effect of synthetic indole phytoalexin derivatives on human colorectal cancer cell lines. METHODSChanges in cell proliferation and the cytotoxic effect of the tested compounds on human colorectal cancer cell lines and human fibroblasts were evaluated using MTS and BrdU assay, allowing us to choose the most potent substance. Cell cycle alterations were analyzed using flow cytometric analysis. The apoptosis-inducing effect of compound K-453 on the HCT116 cell line was examined with annexin V/PI double staining using flow cytometry, as well as acridine orange/propidium iodide (AO/PI) staining. The flow cytometry method also allowed us to measure changes in levels or activation states of other factors associated with apoptosis, such as poly (ADP-ribose) polymerase (PARP), caspase-3 and -9, cytochrome c, Bcl-2 family proteins, and also the integrity of the mitochondrial membrane. To evaluate activity of the transcription factors and proteins involved in signaling pathways we used Western blot analysis together with flow cytometry. RESULTSAmong the ten tested compounds, compound K-453 {(±)-trans-1,2-dimethoxy-2’-(3,5-bis-trifluoromethylphenylamino)spiro{indoline-3,5’[4’,5’]dihydrothiazol} exhibited the most potent activity with IC50 = 32.22 ± 1.14 μmol/L in human colorectal HCT116 cells and was thus selected for further studies. Flow cytometric analysis revealed a K-453-induced increase in the population of cells with sub-G1 DNA content, which is considered as a marker of apoptotic cell death. The apoptosis-inducing effect of compound K453 was also confirmed by annexin V/PI double staining and AO/PI staining. The apoptosis was associated with the loss of mitochondrial membrane potential, PARP cleavage, caspase-3 and caspase-9 activation, release of cytochrome c, as well as changes in the levels of Bcl-2 family members. Moreover, flow cytometry showed that compound K-453 stimulates phosphorylation

关键词： Colorectal carcinoma Indole phytoalexins Antiproliferative Apoptosis Intrinsic pathway

Physico-chemical properties of 3-methoxy-2-nitrobenzoates of some rare earth elements(Ⅲ)

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Journal of Rare Earths 2012年第3期30卷 262-268页

作者： Wiesawa Ferenc Beata Cristóvao jan Sarzyński Halina Guchowska Faculty of Chemistry Maria Curie-Skodowska University Institute of Physics Maria Curie-Skodowska University

The complexes of 3-methoxy-2-nitrobenzoates of Pr（Ⅲ）, Nd（Ⅲ）, Sm（Ⅲ）, Eu（Ⅲ）, Gd（Ⅲ）, Tb（Ⅲ）, Er（Ⅲ） and Tm（Ⅲ） with the formula： Ln（C8H6NO5）3·2H2O, where Ln=lanthanides（Ⅲ）, were synthesized and characterized by elemental analysis, Forier transform irtrared （FTIR） spectroscopy, magnetic and thermogravimetric studies and also by X-ray diffraction （XRD） measurements. The complexes had col- ours typical for Ln（Ⅲ） ions. The carboxylate groups bound as bidentate chelating. On heating to 1173 K in air they decomposed in the same way, at first, dehydrated in one step to anhydrous salts, and then decomposed to the oxides of respective metals with intermediate formation of the oxycarbonates. The enthalpy values of the dehydration process changed from 133.72 to 44.50 kJ/mol. Their solubility in water at 293 K was of the order of 10-4 mol/dm3. The magnetic moments of analysed complexes were determined by Gouy＇s method in the range of 76-303 K.

关键词： 3-methoxy-2-nitrobenzoates thermal stability magnetic properties rare earths

Influence of nitrogen and sulfur fertilization on quality of canola(Brassica napus L.) under rainfed conditions

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第10期8卷 731-737页

作者： AHMAD G. jan A. ARIF M. jan M.T. KHATTAK R.A. Cereal Crops Research Institute (CCRI) Pirsabak North West Frontier Province (NWFP) 25000 Pakistan Department of Agronomy Agricultural University Peshawar North West Frontier Province (NWFP) 25000 Pakistan Dean Faculty of Crop Production Sciences Agricultural University Peshawar North West Frontier Province (NWFP) 25000 Pakistan

Field experiments were conducted at Cereal Crops Research Institute, Pirsabak, Nowshera, Pakistan, during winter 2003～2004; 2004～2005 to evaluate the effect of nitrogen; sulfur levels; methods of nitrogen application on canola (Brassica napus L. cv. Bulbul-98) under rainfed conditions. Four levels of S (0, 10, 20,; 30 kg/ha); three levels of N (40, 60,; 80 kg/ha); a control treatment with both nutrients at zero level were included in the experiments. Sulfur levels were applied at sowing while N levels were applied by three methods (100% soil application, 90% soil+10% foliar application,; 80% soil +20% foliar application). The experiments were laid out in randomized complete block (RCB) design having four replications. Oil content increased significantly up to 20 kg S/ha but further increase in S level did not enhance oil content. Glucosinolate content increased from 13.6 to 24.6 μmol/g as S rate was increased from 0 to 30 kg/ha. Protein content increased from 22.4% to 23.2% as S rate was increased from 0 to 20 kg/ha. Oil content responded negatively to the increasing N levels. The highest N level resulted in the highest values for protein (23.5%); glucosinolate (19.9 μmol/g) contents. Methods of N application had no significant impact on any parameters under study.

关键词： Canola Nitrogen (N) Sulfur (S) Methods of N application Quality

Removal of Rhodamine B from aqueous solutions and wastewater by walnut shells： kinetics, equilibrium and thermodynamics studies

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Frontiers of Chemical Science and Engineering 2013年第4期7卷 428-436页

作者： Jasmin Shah M. Rasul jan Attaul Haq Younas Khan Institute of Chemical Sciences University of Peshawar Peshawar Pakistan

An adsorption study ofRhodamine B （RB） dye from aqueous solutions was carried out using walnut shells pretreated by different methods. In addition to the effects of the pretreatment, the effects of various parameters like pH, adsorbent dose, contact time, initial dye concentration and temperature on the adsorption of RB was studied. The adsorption process was highly pH dependent and a maximum adsorption was achieved at pH 3.0. The best fit for the rates of dye adsorption was a pseudo-second- order kinetic model with good correlation coefficients （R2〉 0.99）. Langmuir isotherms were used to determine that the maximum loading capacity of the different walnut shells and the RB capacities ranged from 1.451-2.292 mg ~ gl. The dye adsorption was also evaluated thermodyna- mically. Positive standard enthalpy （M-P） values were obtained indicating that the RB adsorption process is endothermic as well as AG~ and AS~ values showed that adsorption process is spontaneous with an increased randomness at the solid-liquid interface~ Desorption studies were carried out to explore the feasibility of regenerating the used walnut shells and it was found that 97~71%-99.17% of the retained RB was recovered with 0.1 mol＂ L 1 NaOH solution. The walnut shells were also successfully used to remove RB from industrial effluents.

关键词： Rhodamine B walnut shell adsorption,kinetics isotherms regeneration

Design,synthesis and in vitro evaluation of mono(2,2,2-trifluoroethyl)esters,mono L-amino acid ester prodrugs of acyclic nucleoside phosphonates as anti-HBV agents

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Chinese Chemical Letters 2011年第12期22卷 1387-1390页

作者： Xiao Zhong Fu Yu Ou jan Xin Yu She Yang School of Pharmacy Guiyang Medical CollegeGuiyang 550004China Shanghai Fudan-Yueda Bio-Tech Co. Ltd.Shanghai 201203China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaShanghai Institute for Biological Sciences Chinese Academy of ScienceShanghai 201203China

A series of novel mono（2,2,2-trifluoroethyl） esters,mono L-amino acid ester prodrugs of acyclic nucleoside phosphonates was synthesized and their in vitro anti-HBVactivity was evaluated in HepG 2 2.2.15 *** 1d exhibited more potent anti-HBV activity and lower cytotoxicity than those of adefovir dipivoxil and alamifovir（MCC-478） with EC_（50） and CC_（50） values of 0.01μmol/L and 8000μmol/L respectively.

关键词： Acyclic nucleoside phosphonates L-Amino acid Prodrug