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Adenosine-treated bioprinted muscle constructs prolong cell survival and improve tissue formation

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Bio-Design and Manufacturing 2021年第3期4卷 441-451页

作者： Dongxu Ke Adam M.jorgensen Sang j.Lee james j.yoo Sean V.Murphy Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNCUSA Novaprint Therapeutics Suzhou Co. LtdSuzhouJiangsuChina

It is crucial to maintaining the viability of biofabricated human-sized tissues to ensure their successful survival and function after *** is a purine nucleoside that has the function to suppress cellular metabolism and has been previously proposed as a method to prolong cell viability under *** this study,we optimized the dose concentration of adenosine for incorporation into bioprinted constructs to preserve long-term cell viability in *** results showed that muscle cells(C2C12)containing 6,7,or 8 mM adenosine maintained high cell viability for 20 days under hypoxic conditions(0.1%O2),whereas cells without adenosine treatment showed 100%cell death after 11 *** 20 days under hypoxic conditions,muscle cells treated with adenosine proliferated and differentiated when transferred to normoxic *** these adenosine concentrations,6 mM was picked as the optimized adenosine concentration for further investigations due to its most effective results on improving cell *** bioprinted muscle constructs containing adenosine(6 mM)maintained high cell viability for 11 days under hypoxic conditions,while the control constructs without adenosine had no live *** in vivo validation,the bioprinted constructs with adenosine implanted under the dorsal subcutaneous space in mice,showed the enhanced formation of muscle tissue with minimal central necrosis and apoptosis,when compared to the constructs without *** positive in vitro and in vivo results demonstrate that the use of adenosine is an effective approach to preventing the challenge of hypoxia-induced necrosis in bioprinted tissues for clinical translation.

关键词： Bioprinting Hypoxia Adenosine Cell viability In vivo

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Skin bioprinting:the future of burn wound reconstruction?

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Burns & Trauma 2019年第1期7卷 25-36页

作者： Mathew Varkey Dafydd O.Visscher Paul P.Mvan Zuijlen Anthony Atala james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineMedical Center BoulevardWinston-SalemNC 27101USA Department of Plastic Reconstructive and Hand SurgeryAmsterdam University Medical Center1081HVAmsterdamThe Netherlands Amsterdam Movement Sciences AmsterdamThe Netherlands Burn Center Red Cross Hospital1942LEBeverwijkThe Netherlands Association of Dutch Burn Centres 1942LEBeverwijkThe Netherlands

Burns are a significant cause of trauma,and over the years,the focus of patient care has shifted from just survival to facilitation of improved functional ***,burn treatment,especially in the case of extensive burn injuries,involves surgical excision of injured skin and reconstruction of the burn injury with the aid of skin *** skin substitutes do not contain all skin cell types and do not facilitate recapitulation of native skin ***-dimensional(3D)bioprinting for reconstruction of burn injuries involves layer-by-layer deposition of cells along with scaffolding materials over the injured *** bioprinting can be done either in situ or in *** these approaches are similar except for the site of printing and tissue *** are technological and regulatory challenges that need to be overcome for clinical translation of bioprinted skin for burn ***,the use of bioprinting for skin reconstruction following burns is promising;bioprinting will enable accurate placement of cell types and precise and reproducible fabrication of constructs to replace the injured or damaged ***,3D bioprinting is a very transformative technology,and its use for wound reconstruction will lead to a paradigm shift in patient *** this review,we aim to introduce bioprinting,the different stages involved,in vitro and in vivo skin bioprinting,and the various clinical and regulatory challenges in adoption of this technology.

关键词： Bioprinting Skin Burns Reconstruction

Bioreactor design and validation for manufacturing strategies in tissue engineering

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Bio-Design and Manufacturing 2022年第1期5卷 43-63页

作者： Diana Lim Eric S.Renteria Drake S.Sime Young Min ju ji Hyun Kim Tracy Criswell Thomas D.Shupe Anthony Atala Frank C.Marini Metin N.Gurcan Shay Soker joshua Hunsberger james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNC 27157USA Center for Biomedical Informatics Wake Forest School of MedicineWinston-SalemNC 27157USA RegenMed Development Organization(ReMDO) Winston SalemNC 27106USA

The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore,maintain or improve tissue function following disease or *** maximize the biological function of a tissue-engineered clinical product,specific conditions must be maintained within a bioreactor to allow the maturation of the product in preparation for ***,the bioreactor should be designed to mimic the mechanical,electrochemical and biochemical environment that the product will be exposed to in ***-time monitoring of the functional capacity of tissue-engineered products during manufacturing is a critical component of the quality management *** present review provides a brief overview of bioreactor engineering *** addition,strategies for bioreactor automation,in-line product monitoring and quality assurance are discussed.

关键词： Bioreactor Monitoring sensors and automation Manufacturing Tissue engineering Regenerative medicine

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组织工程中肾脏血管化构建的领先方法（英文）

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Engineering 2022年第12期 117-127+6页

作者： Diana S.Lim john D.jackson Anthony Atala james j.yoo Wake Forest Institute for Regenerative Medicine Wake Forest University School of MedicineMedical Center Boulevard

由于肾衰竭发病率升高的速度远超治疗方法的进展速度,因而对这种疾病新的治疗方法的需求也是以前所未有的速度不断增加。目前的治疗受制于可供应的活器官,而世界范围内均缺乏这种器官。这些治疗模式还需要大量的基础结构,这也极大地限...

由于肾衰竭发病率升高的速度远超治疗方法的进展速度,因而对这种疾病新的治疗方法的需求也是以前所未有的速度不断增加。目前的治疗受制于可供应的活器官,而世界范围内均缺乏这种器官。这些治疗模式还需要大量的基础结构,这也极大地限制了大多数国家的患者获得医疗服务的机会。通过肾脏组织工程方法有望开发出替代的解决方案,解决目前治疗中仍存在的许多不足之处。尽管在生物制造和全器官组织工程方面已经取得了许多进展——主要是在过去的十年中取得的,但仍然存在许多挑战。当前组织工程方法进展中的一个主要障碍是如何对已形成的工程化组织构建体实施成功的血管化。本文主要涉及近年来解决血管问题取得的进展,包括脉管系统的生物制造、通过脱细胞和再细胞化方法实施全器官工程、微尺度器官形成,以及在肾脏组织工程背景下以类器官取得血管化。我们还着重探讨了在制定成功的临床转化策略中仍然存在的具体问题。

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Particle elasticity influences polymeric artificial antigen presenting cell effectiveness in vivo via CD8+T cell activation,macrophage uptake,and the protein corona

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Nano Research 2024年第10期17卷 9052-9064页

作者： Savannah E.Est-Witte Sydney R.Shannon Dennis H.Gong Kaitlyn G.Calabresi jawaun j.Harris Kaitlyn Storm Edwin j.yoo Ariel Isser Vivek P.jani Natalie K.Livingston Mary O.Omotoso Kelly Rhodes Elana Ben-Akiva Randall A.Meyer Zoe T.Hsieh Simone Sidoli Stephany Y.Tzeng jonathan P.Schneck jordan j.Green Translational Tissue Engineering Center Institute for NanobiotechnologyDepartment of Biomedical EngineeringJohns Hopkins School of Medicine400 N BroadwayBaltimoreMD 21231USA Institute for Cell Engineering Department of PathologyJohns Hopkins School of Medicine733 N BroadwayBaltimoreMD 21205USA Department of Biochemistry Albert Einstein College of MedicineThe BronxNew York CityNY 10461USA

Adoptive cell therapy(ACT)is an immunotherapy strategy for cancer that has seen widespread clinical *** ACT,patient-derived lymphocytes are stimulated with the antigen of interest ex vivo,proliferated,then returned to the patient to initiate an antigen-specific antitumor *** effective,this process is resource-intensive and logistically impossible for many *** artificial antigen presenting cells(aAPCs)offer a potential“off-the-shelf”alternative to ex vivo *** particulate aAPCs perform well in vitro,they have had limited success in vivo due to poor bioavailability after *** to bioavailability include rapid clearance,unfavorable biodistribution,and inadequate interactions with CD8+T cells at sites of *** properties such as elasticity have been shown to vastly impact the bioavailability and particle-cell interactions,but this has yet to be investigated in the context of aAPCs for in vivo T-cell *** literature likewise indicates that biomaterial properties,especially elasticity,can modulate T-cell activation in *** the goal of creating a more biomimetic,next-generation particulate aAPC,we developed a poly(ethylene)glycol hydrogel particle platform with tunable elasticity to investigate the impact of elasticity on antigen-specific T cell activation for in vivo adoptive *** this knowledge,we were able to gain more precise control over in vivo T cell activation and investigate possible mechanisms including the effects of aAPC elasticity on T cell binding,macrophage uptake,and the protein corona.

关键词： adoptive cell therapy artificial antigen presenting cells particle drug delivery elasticity protein corona poly(ethylene glycol)

An analytical model to estimate the time delay to reach spontaneous ignition considering heat loss in oil reservoirs

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Petroleum Science 2024年第4期21卷 2469-2474页

作者： james j.Sheng Er-Long Yang College of Petroleum Engineering Northeast Petroleum University

During air injection into an oil reservoir, an oxidation reaction generates some heat to raise the reservoir temperature. When the reservoir temperature reaches an ignition temperature, spontaneous ignition occurs. There is a time delay from the injection to ignition. There are mixed results regarding the feasibility of spontaneous ignition in real-field projects and in laboratory experiments. No analytical model is available in the literature to estimate the oxidation time required to reach spontaneous ignition with heat *** paper discusses the feasibility of spontaneous ignition from theoretical points and experimental and field project observations. An analytical model considering heat loss is proposed. Analytical models with and without heat loss investigate the factors that affect spontaneous ignition. Based on the discussion and investigations, we find that it is more difficult for spontaneous ignition to occur in laboratory experiments than in oil reservoirs; spontaneous ignition is strongly affected by the initial reservoir temperature, oil activity, and heat loss; spontaneous ignition is only possible when the initial reservoir temperature is high, the oil oxidation rate is high, and the heat loss is low.

关键词： Spontaneous ignition Time delay Air injection Heat loss Low temperature oxidation Combustion

Evaluating reservoir suitability for large-scale hydrogen storage:A preliminary assessment considering reservoir properties

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Energy Geoscience 2024年第4期5卷 198-211页

作者： Chinedu j.Okere james j.Sheng Chinedu Ejike Bob L.Herd Department of Petroleum Engineering Texas Tech UniversityLubbockTX79409United States Mewbourne School of Petroleum and Geological Engineering University of OklahomaOK73019United States

With rising demand for clean energy,global focus turns to finding ideal sites for large-scale underground hydrogen storage(UHS)in depleted petroleum reservoirs.A thorough preliminary reservoir evaluation before hydrogen(H_(2))injection is crucial for UHS success and *** criteria for UHS often emphasize economics and chemistry,neglecting key reservoir *** study introduces a comprehensive framework for the reservoir-scale preliminary assessment,specifically tailored for long-term H_(2) storage within depleted gas *** evaluation criteria encompass critical components,including reservoir geometry,petrophysical properties,tectonics,and formation *** illustrate the practical application of this approach,we assess the Barnett shale play reservoir *** assessment unfolds through three key stages:(1)A systematic evaluation of the reservoir's properties against our comprehensive screening criteria determines its suitability for H_(2) storage.(2)Using both homogeneous and multilayered gas reservoir models,we explore the feasibility and efficiency of H_(2) *** phase involves an in-depth examination of reservoir behavior during the injection stage.(3)To enhance understanding of UHS performance,sensitivity analyses investigate the impact of varying reservoir dimensions and injection/production *** findings reveal the following:(a)Despite potential challenges associated with reservoir compaction and aquifer support,the reservoir exhibits substantial promise as an H_(2) storage site.(b)Notably,a pronounced increase in reservoir pressure manifests during the injection stage,particularly in homogeneous reservoirs.(c)Furthermore,optimizing injection-extraction cycle efficiency can be achieved by augmenting reservoir dimensions while maintaining a consistent *** ensure a smooth transition to implementation,further comprehensive investigations are advised,including experimental and numerical studies to address

关键词： Site selection Underground hydrogen storage Preliminary evaluation Depleted petroleum reservoirs Reservoir assessment

Structural performance of flexible freeform panelssubjected to wind loads

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Frontiers of Structural and Civil Engineering 2024年第6期18卷 851-869页

作者： Yong yoo Zaryab SHAHID Renzhe CHEN Maria KOLIOU Anastasia MULIANA Negar KALANTAR Zachry Department of Civil and Environmental Engineering Texas A&M UniversityCollege StationTX 77843USA j.Mike Walker'66 Department of Mechanical Engineering Texas A&M UniversityCollege StationTX 77843USA Architecture Division California College of the ArtsSan FranciscoCA 94107USA

An increased number of hurricanes and tornadoes have been recorded worldwide in the last decade,while research efforts to reduce wind-related damage to structures become *** architecture,which focuses on generating complex curved shapes including streamlined shapes,has recently gained *** study focuses on investigating the potential of kerf panels,which have unique flexibility depending on the cut patterns and densities,to generate complex shapes for facades and their performance under wind *** investigate the kerf panel's potential capacity against wind loads,static and dynamic analyses were conducted for two kerf panel types with different cut densities and pre-deformed *** was observed that although solid panels result in smaller displacement amplitudes,stresses,and strains in some cases,the kerf panels allow for global and local cell deformations resulting in stress reduction in various locations with the potential to reduce damage due to overstress in *** the predeformed kerf panels,it was observed that both the overall stress and strain responses in kerf cut arrangements were lower than those of the flat-shaped *** study shows the promise of the use of kerf panels in achieving both design flexibility and performance demands when exposed to service *** that this newly proposed architectural configuration(design paradigm)for facades could revolutionize structural engineering by pushing complex freeform shapes to a standard practice that intertwines aesthetic arguments,building performance requirements,and material design considerations has the potential for significant practical applications.

关键词： freeform structure kerfing method wind load analysis cladding component

Insm1:orchestrating cellular mimicry in the thymus medulla

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Cellular & Molecular Immunology 2024年第4期21卷 414-416页

作者： K.D.james j.E.Cowan UCL Institute of Immunity and Transplantation The Pears BuildingRowland Hill StreetLondonNW32PPUK

The thymus is the primary production site for a functional and self-tolerant T-cell *** establishment of immune tolerance is mediated by the generation and presentation of tissue-restricted self-antigens(TRAs)by medullary thymic epithelial cells(mTECs),which screen T-cell receptors for self-reactivity to eliminate autoreactive T *** studies have extended our understanding of mTEC heterogeneity by examining the mechanisms by which mTECs mimic the peripheral self[1].

关键词： medullary eliminate restricted