Adenosine-treated bioprinted muscle constructs prolong cell survival and improve tissue formation

作     者：Dongxu Ke Adam M.Jorgensen Sang J.Lee James J.Yoo Sean V.Murphy Dongxu Ke;Adam M.Jorgensen;Sang J.Lee;James J.Yoo;Sean V.Murphy

作者机构：Wake Forest Institute for Regenerative MedicineWake Forest School of MedicineWinston-SalemNCUSA Novaprint Therapeutics Suzhou Co.LtdSuzhouJiangsuChina 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2021年第4卷第3期

页      面：441-451页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0817[工学-化学工程与技术] 08[工学] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0836[工学-生物工程] 0811[工学-控制科学与工程] 

基　　金：funding from the Armed Forces Institute for Regenerative Medicine (AFIRM) under the grant 

主　　题：Bioprinting Hypoxia Adenosine Cell viability In vivo 

摘      要：It is crucial to maintaining the viability of biofabricated human-sized tissues to ensure their successful survival and function after *** is a purine nucleoside that has the function to suppress cellular metabolism and has been previously proposed as a method to prolong cell viability under *** this study,we optimized the dose concentration of adenosine for incorporation into bioprinted constructs to preserve long-term cell viability in *** results showed that muscle cells(C2C12)containing 6,7,or 8 mM adenosine maintained high cell viability for 20 days under hypoxic conditions(0.1%O2),whereas cells without adenosine treatment showed 100%cell death after 11 *** 20 days under hypoxic conditions,muscle cells treated with adenosine proliferated and differentiated when transferred to normoxic *** these adenosine concentrations,6 mM was picked as the optimized adenosine concentration for further investigations due to its most effective results on improving cell *** bioprinted muscle constructs containing adenosine(6 mM)maintained high cell viability for 11 days under hypoxic conditions,while the control constructs without adenosine had no live *** in vivo validation,the bioprinted constructs with adenosine implanted under the dorsal subcutaneous space in mice,showed the enhanced formation of muscle tissue with minimal central necrosis and apoptosis,when compared to the constructs without *** positive in vitro and in vivo results demonstrate that the use of adenosine is an effective approach to preventing the challenge of hypoxia-induced necrosis in bioprinted tissues for clinical translation.