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<i>Agrobacterium</i>-Mediated Transformation of Mexican Lime (<i>Citrus aurantifolia</i>Swingle) Using Optimized Systems for Epicotyls and Cotyledons

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Advances in Bioscience and Biotechnology 2015年第11期6卷 657-668页

作者： Maria Luiza P. de Oliveira Gloria Moore james G. thomson Ed Stover USDA-ARS Subtropical Insects and Horticulture Research Unit Fort Pierce USA Horticultural Science Department Institute of Food and Agricultural Science University of Florida Gainesville USA USDA-ARS Crop Improvement and Utilization Albany USA

Transgenic Mexican lime (Citrus aurantifolia Swingle) was produced through two explant sources, each using systems previously optimized for each source. One used epicotyls segments, which was the predominant explant for transgenic Citrus production following co-cultivation with Agrobacterium, and has a well-established protocol. The other procedure used embryo cotyledons from mature seeds, which was developed in our lab as an alternative for stable Citrus transformation. Cotyledon transformation and regeneration protocols were optimized by comparing variables in culture medium composition on shoot regeneration and four parameters in transient transformation. The optimized protocols were compared, and frequency of regeneration, frequency of transgenic plant-recovery and stable transformation efficiency indicated the superiority of the cotyledon protocol for Agrobacterium-mediated genetic transformation in Mexican lime. The tissue choice resulted in marked improvement in shoot regeneration (14.1% of explants producing shoots in epicotyls;55.8% in cotyledons), stable transformation frequency (11.4% of epicotyls explants;40.2% in cotyledons), and frequency of transgenic plant-recovery (37.9% in epicotyl explants;92.6% in cotyledons). Thus, easy availability of explants using embryo cotyledons from mature seeds, technical simplicity, shortening of transformation time-course, and higher transformation and regeneration frequencies makes this new system an attractive alternative over the previously published Citrus transformation protocols. In the course of this project, we generated Mexican lime with a Recombinase Mediated Exchange Cassette landing pad, which was designed for stacking transgenes.

关键词： Agrobacterium tumefaciens Embryo Cotyledons Epicotyls Segments Recombinase Mediated Exchange Cassette Tissue Culture

Handbook of Stem Cells

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丛书名： v. 1

2004年

作者： Robert Lanza John Gearhart Brigid Hogan Douglas Melton Roger Pedersen james thomson Michael West

ISBN: (数字)9780080533735

ISBN: (纸本)9780124366435

New discoveries in the field of stem cell research have frequently appeared in the news and in scientific literature. Research in this area promises to lead to new therapies for cancer, heart disease, diabetes, and a wide variety of other diseases. This two-volume reference integrates this exciting area of biology, combining the prerequisites for a general understanding of adult and embryonic stem cells, the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations, as well as a presentation by the world's experts of what is currently known about each specific organ system. The editors of the Handbook of Stem Cells include: Robert Lanza, Helen Blau, John Gearhart, Brigid Hogan, Douglas Melton, Malcolm Moore, Roger Pedersen, E. Donnall Thomas, james thomson, Catherine Verfaillie, Irving Weissman, and Michael West. The Editorial Board includes: W. French Anderson, Peter Andrews, Anthony Atala, Jose Cibelli, Giulio Cossu, Robert Edwards, Martin Evans, Elaine Fuchs, Margaret Fuller, Fred Gage, Richard Gardner, Margaret Goodell, Ronald Green, William Haseltine, Joseph Itskovitz-Eldor, Rudolf Jaenisch, Ihor Lemischka, Dame Anne McLaren, Richard Mulligan, Stuart Orkin, Martin Pera, Benjamin Reubinoff, Janet Rossant, Hans Scholer, Austin Smith, Evan Snyder, Davor Solter, Alan Trounson, and Leonard Zon. This comprehensive set should be a much-needed addition to the library of students and researchers alike.

关键词： Stem cells.

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使用水平定向钻进技术安装海洋排水管口

非开挖技术 2017年第1期 15-17页

作者： james thomson 徐晗陈晨

海洋排污口的设计与施工是非常重要的，涉及到很多的专业知识。污水的排放要经过预处理，而不是直接排放。运用非开挖技术安装海洋废水排污口是一项很好的选择，包括微型隧道技术和水平定向钻。本文介绍了水平定向钻进安装海洋排水管口... 详细信息

海洋排污口的设计与施工是非常重要的，涉及到很多的专业知识。污水的排放要经过预处理，而不是直接排放。运用非开挖技术安装海洋废水排污口是一项很好的选择，包括微型隧道技术和水平定向钻。本文介绍了水平定向钻进安装海洋排水管口的施工技术。

关键词：排水系统海洋水平定向钻进

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美中关系史论

作者：欧内斯特·梅(Ernest R. May) 小詹姆斯·汤姆逊(james C. thomson)

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亚马逊该如何巧取轻奢市场

新营销 2018年第1期20卷 6-6页

作者： james thomson

https://***/亚马逊一直在为平台招揽轻奢品牌。事实上,要想获取更多轻奢消费者,亚马逊可以采取这样一种方法:推出垂直整合、大规模定制的自营品牌,采取技术手段优化客户购物流程,就像Indochino(加拿大在线定制男装电商)那样。虽然亚马... 详细信息

https://***/亚马逊一直在为平台招揽轻奢品牌。事实上,要想获取更多轻奢消费者,亚马逊可以采取这样一种方法:推出垂直整合、大规模定制的自营品牌,采取技术手段优化客户购物流程,就像Indochino(加拿大在线定制男装电商)那样。虽然亚马逊不太可能跟那些高端奢侈品牌抢客户,但它的确能通过算法降低轻奢品牌与其用户之间的关联度,甚至取而代之,推出自营奢侈品牌。

关键词：亚马逊奢侈品牌自营品牌

Recent advances in celiac disease

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World Journal of Gastroenterology 2011年第18期17卷 2259-2272页

作者： Hugh james Freeman Angeli Chopra Michael Tom Clandinin Alan BR thomson Division of Gastroenterology University of British Columbia Division of General Internal Medicine University of Alberta Department of Agricultural Food and Nutritional Science University of Alberta

Celiac disease now affects about one person in a hundred in Europe and North America. In this review, we consider a number of important and exciting recent developments, such as clinical associations, HLA-DQ2 and HLA-DQ8 predispositions, the concept of potential celiac disease, the use of new imaging/endoscopy techniques, and the development of refractory disease. This review will be of use to all internists, pediatricians and gastroenterologists.

关键词： Inflammation Infection Malabsorption Pathophysiology Physiology

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向前

时代英语（高一版） 2017年第2期 1-1页

作者：詹姆斯·汤姆逊

火车载着我们朝前急驶。树木和房屋向后飞逝．原野上星空一片灿烂。沿着铁轨扑面而至。满天的星斗多么美丽。犹如夜空之林中银色的鸽子．在朦胧的大地上成群聚集。陪同我们一起腾空翱翔。

关键词：高中英语课外阅读阅读材料

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马克士威

Remote Sensing of Selected Winter and Spring Host Plants of Tarnished Plant Bug (Heteroptera: Miridae) and Herbicide Use Strategies as a Management Tactic

丛书名：自然科学小丛书

1936年

作者：（英）J.J.thomson等周麟译

书名原文:james Clark Maxwell:A commemoration Volume:译文集。内收《詹姆士克娄克马克士威》（J.J.汤姆孙）、《马克士威对于德国理论物理学的影响》（蒲郎克）、《马克士威与物理的实在概念之发展》（爱因斯坦）、《马克士威的科学... 详细信息

书名原文:james Clark Maxwell:A commemoration Volume:译文集。内收《詹姆士克娄克马克士威》（J.J.汤姆孙）、《马克士威对于德国理论物理学的影响》（蒲郎克）、《马克士威与物理的实在概念之发展》（爱因斯坦）、《马克士威的科学环境》（拉默尔）、《马克士威的方法》（詹斯）、《马克士威的实验室》（加奈特）、《对于马克士威教授的一些回忆》（夫丽明）、《马克士威与无线电报》（洛治）、《卡文迪虚实验室的初年》（格奈慈不鲁克）、《作为讲师的克娄克马克士威》（兰布）等10篇文章。

关键词：马克斯韦尔学科:研究

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American Journal of Plant Sciences 2015年第8期6卷 1313-1327页

作者： Donald L. Sudbrink Steven J. thomson Reginald S. Fletcher F. Aubrey Harris Patrick J. English james T. Robbins Austin Peay State University Department of Agriculture Clarksville USA United States Department of Agriculture Agricultural Research Service Crop Production Systems Research Unit Stoneville USA Delta Research and Extension Center Mississippi State University Stoneville USA

Remote sensing was used in a series of experiments over a three-year period to obtain spectral reflectance data for studying differences in vegetation indices between grasses, broadleaf plants, and grass/broadleaf plant mixtures. Selected non-crop winter and spring host plants of tarnished plant bug, Lygus lineolaris, were planted in field-plot experiments. Multispectral reflectance data were aerially acquired with both a Real-Time Digital Airborne Camera System (RDACS) sensor and Geospatial Systems (Duncan Tech) MS-2100 multispectral camera. The following six vegetation indices of spectral reflectance were evaluated in this study: normalized difference vegetation index, ratio vegetation index, green normalized difference vegetation index, green vegetation index, green ratio vegetation index, and Ashburn vegetation index. Vegetation indices calculated with imagery data for the grasses and broadleaves differed significantly;there appeared to be more discriminating differences between vegetation indices for grasses and broadleaf plants when the indices were based on a combination of green and near infrared or green and red spectral bands than when the indices were based on red and near infrared spectral bands. Insect data from these studies confirm that tarnished plant bug prefers broadleaf host plants, but can use Italian ryegrass for food and reproduction. The narrow temporal window of host suitability for Italian ryegrass may limit its significance. Herbicide destruction of broadleaf host plants in early spring prevented the tarnished plant bug population increases that occurred in untreated plots. Findings of this study would be useful in site-specific vegetation management decisions in programs such as the prototypical area-wide tarnished plant bug management experiment conducted in the Delta region of Mississippi.

关键词： Remote Sensing Vegetation Index Lygus lineolaris Area-Wide Management