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Seroprevalence of toxoplasmosis and rubella in pregnant women attending antenatal private clinic at Ouagadougou,Burkina Faso

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Asian Pacific Journal of Tropical Medicine 2012年第10期5卷 810-813页

作者： Laure Stella Ghoma Linguissi Bolni Marius Nagalo Cyrille Bisseye Thrse S Kagon Mahamoudou Sanou Issoufou Tao Victoire Benao jacques simpor Bibiane Kon Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA/LABIOGENE) UFR/SVTUniversite de OuagadougouBurkina Faso Departement de Biologie Universite des Sciences et Techniques de MasukuFrancevilleGabon Centre MURAZ Bobo-DioulassoBurkina Faso Centre National de Transfusion Sanguine(CNTS) Ouagadougou.Burkina Faso Clinique Moussa Kone OuagadougouBurkina Faso Centre Medical Saint Camille OuagadougouBurkina Faso

Objective:To evaluate the prevalence of toxoplasmosis and rubella among pregnant women at Ouagadougou in Burkina ***:All patient sera were tested for rubella and toxoplasmosis anti-IgG using commercial ELISA kits(PlateliaTMRubella IgG and Platelia? Toxo IgG).The presence of anti-rubella and anti-toxoplasmosis tgM in serum samples was tested using commercial ELISA kits Platelia Rubella TgM and Platelia Toxo ***:Among all the pregnant women tested for toxoplasmosis and rubella,their prevalence were 20.3% and 77.0%,*** women in the age group of 18-25 years showed the highest frequency of anti-toxoplasmosis（34.5%） and anti-rubella IgG（84.6%）.The prevalence of anti-toxoplasma and anti-rubella IgG decreased between 2006 and 2008 from 32.7%to 12.1%and 84.6% to 65.0%,*** was no significant association between age and the mean titer of anti-toxoplasmosis IgG among pregnant ***:The diagnosis of toxoplasmosis and rubella is necessary in pregnant women in Burkina Faso because of the low immunization coverage rate of rubella and the high level of exposure to these two infections which can be harmful to the newborn if contracted by women before the third trimester of pregnancy.

关键词： IgG Toxoplasma gondii Rubella Seroprevalence Burkina Faso

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G6PD Deficiency and COVID-19 in Burkina Faso: A Possible Link?

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Journal of Biosciences and Medicines 2023年第1期11卷 57-69页

作者： Abdoul Karim Ouattara Lassina Traoré Tégwendé Rebeca Compaoré Théodora Mahoukèdè Zohoncon jacques simporé Université Norbert Zongo/Centre Universitaire de Manga Koudougou Burkina Faso Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) UFR/SVT Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Département Biomédical et de Santé Publique Institut de Recherche en Sciences de la Santé (CNRST/IRSS) Ouagadougou Burkina Faso Faculté de Médecine Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso

Burkina Faso is a malaria-endemic country, with a high incidence of G6PD deficiency (G6PDd), which recorded its first case of COVID-19 in March 2020. G6PDd leads to a decrease in the efficiency of erythrocytes to combat oxidative stress, while SARS-CoV-2 infection induces massive production of Reactive Oxygen Species (ROS) in patients. In the present review, we discuss a possible link between G6PDd and SARS-CoV-2 infection. The mean prevalence of G6PDd in Burkina Faso is estimated at 16.6% among males and 6.5% among females. A total of 21,128 cases of COVID-19 have been recorded in Burkina Faso with 387 deaths reported (with a mortality rate of 1.15% among diagnosed cases) as of August 30, 2022. To our knowledge, no association study between G6PDd and SARS-CoV-2 infection has been conducted to date in Burkina Faso. However, several case reports around the world have described elevated risks of hemolysis and thrombosis, and other complications among G6PD-deficient patients infected with SARS-CoV-2. The use of Hydroxychloroquine (HCQ) has also been deemed unsafe by some authors for the treatment of COVID-19 among patients with G6PDd. Although HCQ has been shown to be well tolerated in COVID-19 patients in Burkina Faso, the drug could induce hemolytic crises in people with G6PD deficiency. G6PD is important in regulating ROS and maintaining erythrocyte homeostasis. In view of its high prevalence in Burkina Faso, determination of the G6PD status is required in COVID-19 patients for adequate management such as identifying a subset of COVID-19 patients for whom close monitoring and supportive care may be essential and to restrict treatment with HCQ.

关键词： G6PD Deficiency COVID-19 CQ/HCQ Hemolysis Burkina Faso

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Resistance of Klebsiella to Imipenem by Production of Carbapenemase Gene bla_IMP at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso

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International Journal of Clinical Medicine 2023年第8期11卷 347-356页

作者： Blandine Ouédraogo Abdoul Karim Ouattara Amana Mètuor Dabiré Rahimatou Yasmine Wendkuni Tiemtoré Serge Sougé jacques simporé Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) UFR-SVT Université Joseph KI-ZERBO Ouagadougou Burkina Faso Université Norbert Zongo Centre Universitaire de Manga Koudougou Burkina Faso Département de Biochimie-Microbiologie Université de Dédougou Dédougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso

Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene blaIMP in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. Methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with blaIMP-type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the blaIMP-type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.

关键词： Klebsiella Resistance blaIMPGenes β-lactam Burkina Faso

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Can Plasmodium falciparum Induce Homocysteinemia in Malaria Patients?

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Journal of Biosciences and Medicines 2022年第5期10卷 117-128页

作者： Noé Yaméogo Abdoul Karim Ouattara Bapio Valérie Bazié Alfred Rakissida Ouédraogo Florencia Wendkuuni Djigma jacques simporé Laboratoire de Biologie Moléculaires et de Génétique (LABIOGENE) UFR-SVT Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre Hospitalier Universitaire de Bogodogo Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Université Norbert Zongo (UNZ) Centre Universitaire de Manga Koudougou Burkina Faso Centre National de Recherche Scientifique et Technologique Ouagadougou Burkina Faso UFR-SDS Université Joseph KI-ZERBO Ouagadougou Burkina Faso

Background: Plasmodium falciparum has developed elaborate strategies to survive in the hostile intracellular environment of the infected host cell, including resistance to oxidative stress. Cysteine is a metabolic product of homocysteine and a precursor of the antioxidant glutathione used by Plasmodium falciparum to escape harmful oxidation. Objectives: In the present study we aimed to assess whether Plasmodium falciparum can induce homocysteinemia in malaria patients of Burkina Faso. Methods: Eighty-five (85) individuals including 25 affected by severe malaria, 44 by simple malaria, and 12 negative controls for P. falciparum infection were included in the present study. An enzymatic assay of plasma homocysteinemia was performed using the Homocysteine Enzymatic Assay reagent (ref 05385415 190) on the Roche/ Hitachi Cobas c. Results: The results of the present study show that the mean plasma homocysteine concentrations were 15.1 ± 8.4 μmol/L among patients with severe malaria, 14.0 ± 6.0 μmol/L in patients with uncomplicated malaria, and 12.6 ± 4.1 μmol/L in negative controls for malaria parasite. Conclusions: Our findings suggest high homocysteinemia in malaria patients, especially in those with severe malaria. Monitoring homocysteinemia in the latter group will be useful to avoid complications when an elevated plasma level of homocysteine is a known risk factor for cardiovascular diseases.

关键词： Homocysteine Malaria Burkina Faso

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Molecular Detection of Extended-Spectrum Beta Lactamase and Carbapenemase Genes in Escherichia coli and Salmonella spp. Isolated from Chickens Consumed Outdoors in Ouagadougou, Burkina Faso

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Journal of Biosciences and Medicines 2024年第11期12卷 221-232页

作者： Amana Mètuor Dabiré Stéphanie Lynseh Carine Sita Bénao Nicolas Ouédraogo Rahimatou Yasmine Wendkouni Tiemtoré Rhaina Olivia Badini Lionel Eliada Benoit Bambara jacques simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center Department of Biochemistry-Microbiology Joseph Ki-Zerbo University Ouagadougou Burkina Faso Department of Biochemistry-Microbiology University of Ddougou Ddougou Burkina Faso Centre National de Recherche et de Formation sur le Paludisme Institut National de Sant Publique Ouagadougou Burkina Faso Laboratoire de biochimie et immunologie applique (LABIA) Joseph Ki-Zerbo University Ouagadougou Burkina Faso

This study was carried out to assess the prevalence of resistance genes in strains of Escherichia coli and Salmonella spp. isolated from free-range chickens in Ouagadougou, where resistant bacteria can be transmitted to humans via faeces or contaminated meat. A total of 280 strains of Escherichia coli and 129 strains of Salmonella spp. resistant to at least one beta-lactam or carbapenem antibiotic were used in this study. PCR analyses revealed the presence of ESBL (extended spectrum beta lactamase) resistance genes in Escherichia coli isolates, with 3.21% (9/280) possessing the CTX-M (Cefotaximase) gene, 15.35% (43/280) had the SHV (Sulfhydril Variable) gene, and 11.42% (32/280) had carbapenemase resistance genes, more specifically IMP (Imipenemase metallo-beta-lactamase). As regards Salmonella spp. strains, only the presence of the SHV (Sulfhydril Variable) gene was identified in 2.32% (3/129) of isolates belonging to the ESBL family, while 26.35% (34/129) and 13.95% (18/129) of isolates respectively possessed the IMP (Imipenemase metallo-beta-lactamase) and NDM (New Delhi metallo-β-lactamase) genes, both of the carbapenemase type. The significant prevalence of resistance genes in bacterial strains isolated from chickens sold outdoors in Ouagadougou raises major public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： Bacteria Resistance Extended-Spectrum Beta Lactamases Carbapenemases Ouagadougou

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Frequency of Antibiotic Resistance of Escherichia coliand Klebsiella pneumoniaeby Production of TOHO-Type β-Lactamases at Saint Camille Hospital, Ouagadougou (Burkina Faso)

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Advances in Microbiology 2021年第12期11卷 713-722页

作者： Serge Sougué Amana Mètuor-Dabiré Djénéba Ouermi Yasmine Rahimatou Wend-Kouni Tiemtoré Bénao Stéphanie Lynseh Carine Sita Théodora Mahoukèdè Zohoncon Yasmine Aminata Bangré Elie Kabré jacques simporé Department of Biochemistry and Microbiology Faculty of Applied Science and Technology Biomolecular Research Centre (CERBA/LABIOGENE) UFR-SVT Joseph KI-ZERBO University Ouagadougou Burkina Faso National Public Health Laboratory of Ouagadougou Ouagadougou Burkina Faso University of Dedougou Dedougou Burkina Faso Saint Thomas Aquinas University (USTA) Saint Camille Hospital of Ouagadougou Ouagadougou Burkina Faso

Extended-spectrum β-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEM, blaCTX-M and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-M subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in Escherichia coli and Klebsiella pneumoniae in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by Escherichia coli and Klebsiella pneumoniae using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value Escherichia coli and 18 (46.2%) Klebsiella pneumoniae. Molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in Escherichia coli and Klebsiella pneumoniae.

关键词： Antibiotic Resistance Bacteria ESBL Genes TOHO

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Multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

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Open Journal of Applied Sciences 2024年第4期14卷 881-892页

作者： Stéphanie Lynseh Carine Sita Bénao Dabiré Amana Métuor Abdoul Karim Ouattara Rahimatou Yasmine Wendkouni Tiemtoré Nicolas Ouédraogo Blandine Ouédraogo Rhaina Olivia Badini Lionel Eliada Benoit Bambara Serge Sougué jacques simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center (CERBA) Department of Biochemistry-Microbiology University Joseph Ki-Zerbo Ouagadougou Burkina Faso Department of Biochemistry-Microbiology University of D&#233dougou D&#233dougou Burkina Faso National Malaria Research and Training Centre (CNRFP) Ouagadougou Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： Multidrug-Resistant Chicken Ouagadougou Escherichia coli Salmonella spp. Antibiotic

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Antibacterial, Anti-Inflammatory and Antioxidant Activities of an ACAZY Herbal Formula Used in Traditional Medicine to Treat Respiratory Infections

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Natural Science 2024年第9期16卷 131-145页

作者： Mohamed Bonewendé Belemlilga Bagora Bayala Abdoul Gilchrist Laurent Boly Madinatou Annick Ouédraogo Alida Raissa Boina Boubacar Yaro Florentina Badea Camélia Diguti Moumouni Koala Denise P. Ilboudo Charles Bavouma Sombié Pascal Nadembega Jean-Marie Compaoré Paul Darga Mahoukèdè Théodora Zohoncon Moussa Ouédraogo Aristide Traore Noufou Ouédraogo Florentina Matei Rasmane Semdé Sylvin Ouédraogo jacques simporé Innocent Pierre Guissou Laboratoire de Recherche-Dveloppement de Phytomdicaments et Mdicaments (LR-D/PM) Institut de Recherche en Sciences de la Sant (IRSS) Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Centre de Recherche Biomolculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie Molculaire et de Gntique (LABIOGENE) Universit Joseph KI-ZERBO Ouagadougou Burkina Faso Ecole Normale Suprieure Koudougou Burkina Faso Laboratoire de Dveloppement du Mdicament (LADME) Centre de Formation de Recherche et dExpertises en Sciences du Mdicament (CEA-CFOREM) cole Doctorale Sciences de la Sant (ED2S) Universit Joseph KI-ZERBO Burkina Faso Laboratoire de Microbiologie Applique Universit des Sciences Agronomiques et de Mdecine Vtrinaire (UASMV) de Bucarest en Roumanie Bucharest Romania Dpartement de la mdecine traditionnelle Ministre de la Sant et de lHygine Publique Ouagadougou Burkina Faso Universit Saint Thomas dAquin (USTA) Ouagadougou Burkina Faso

ACAZY is a plant formula used in traditional medicine in Burkina Faso to treat respiratory infections. After phytochemical analysis, this study evaluated extracts’ anti-inflammatory, antioxidant and antibacterial properties from the ACAZY recipe. Three extractions, an aqueous macerate (AM), an aqueous decoction (AD) and an hydroethanolic macerate (HEM) of the ACAZY recipe powder were carried out. Phytochemical screening of the extracts was carried out using high-performance thin-layer chromatography (HPTLC) and the determination of phenolic compounds. The anti-inflammatory potential was assessed in vitro using pro-inflammatory enzyme inhibition tests. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric-reducing antioxidant power (FRAP) antioxidant properties were also determined. The antibacterial activity was evaluated on Staphylococcus aureus and Streptococcus pneumoniae strains. Phytochemical analysis revealed the presence of flavonoids, saponins, tannins, anthracenosids, sterols and triterpenes in the extracts. The extracts inhibited pro-inflammatory enzymes by more than 40% at only 100 µg/mL. The extracts also showed potent antibacterial activity with a minimum inhibitory concentration 1 mg/mL on Staphylococcus aureus and 2 mg/mL on Streptococcus pneumoniae. The extracts in the ACAZY formula have shown anti-inflammatory and antioxidant properties in vitro. The AD also showed an antibacterial activity. This justifies its use in traditional medicine to treat acute respiratory infections.

关键词： ACAZY Anti-Inflammatory Antioxidant Antibacterial In Vitro

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