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Screening of Environmental Bacterial Strains for Wild Aedes Mosquito Larval Biocontrol

Open journal of Applied Sciences 2024年第9期14卷 2431-2442页

作者： Alexandre Paulo Machado Liliana Alves Correa Gabriel de Paula Albuquerque Ana Lúcia Maria Ribeiro Rosina Djunko Miyazaki j.ssica da sil.a gava Dyego Bichofre Cruz Laboratory of Microbiology Medical Faculty Federal University of Mato Grosso Mato Grosso Brazil Laboratory of Entomology Institute of Biosciences Federal University of Mato Grosso Mato Grosso Brazil

The effectiveness of current control measures against Aedes mosquitoes remains low, resulting in persistent epidemics in urban areas. The emergence of resistant mosquito populations to chemical insecticides highlights the need for novel, environmentally friendly, cost-effective control strategies. This study explored the potential of environmental bacterial isolates to biocontrol wild Aedes larvae. Initially, we collected bacterial samples from infectious masses of Aedes fluviatilis larvae. The isolated bacteria were identified using biochemical, enzymatic, and molecular methods, including 16S rRNA sequencing and MALDI-TOF. Previously, Aeromonas hydrophila and Bacillus thuringiensis isolated from these infectious masses showed limited Aedes larval inhibition. Consequently, we screened additional environmental isolates from the bacteriotheque. Six isolates previously identified were tested: Chromobacterium violaceum, Enterobacter cloacae, Bacillus cereus, Bacillus sphaericus, and two strains of Bacillus thuringiensis israelensis. Among these strains, B. thuringiensis and C. violaceum exhibited significant inhibitory activities against wild Aedes larvae. Bacillus thuringiensis cultures grown under daylight conditions showed a slight ability to inhibit Aedes larvae. The potential of B. thuringiensis and C. violaceum strains studied, along with optimized culture growth conditions, will be further investigated to develop bioinsecticide products to provide safer and more sustainable alternatives for controlling larvae of Aedes mosquitoes.

关键词： Bacillus thuringiensis Chromobacterium violaceum Aedes Biocontrol Larval Inhibition

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Growth and Quality of Clonal Plantlets of Conilon Coffee(Coffea canephora Pierre ex ***)Influenced by Types of Cuttings

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American journal of Plant Sciences 2014年第14期5卷 2148-2153页

作者： Abraao Carlos Verdin Filho Aldo Luiz Mauri Paulo Sergio Volpi Aymbire Francisco Almeida da Fonseca Romario gava Ferrao Maria Amelia gava Ferrao Wagner Nunes Rodrigues Saul de Andrade júnior Tafarel Victor Colodetti Instituto Capixaba de Pesquisa Assistencia Técnica e Extensao Rural(Incaper) MarilandiaBrazil Empresa Brasileira de Pesquisa Agropecuaria(Embrapa Cafe) BrasiliaBrazil Centro de Ciencias Agrarias Universidade Federal do Espírito Santo(CCA-UFES)AlegreBrazil Escola Estadual Rural de Colatina(EECOR) ColatinaBrazil

To achieve high crop yieldin agriculture, the production of plantlets of superior quality is one of thebasic prerequisites. For conilon coffee, the current recommendation in theproduction of clonal plantlets through vegetative propagation is to usediagonal cuttings, in bevel form. However, there are indications that othertypes of cutting have been successfully used to produce plantlets. In thiscontext, this experiment was conducted with the objective of studying thedevelopment of plantlets of conilon coffee obtained by different types ofcuttings. The experiment was conducted in a greenhouse. The cuttings wereobtained from the middle portion of orthotropic stems of standardized *** experiment followed a split plot in time design, with three types ofcuttings (bevel, sharp bevel and straight) and two times of evaluation, withfour repetitions. At 30 and 120 days after planting, plantlets were collectedand evaluated. The results show the increase occurred in the variables due tothe straight cut when compared to the others, except for stem *** in this context, the straight cut promoted an increase in seedlingheight, production of biomass and in the Dickson’s quality index when comparedto cuttings in bevel and sharp bevel.

关键词： Coffea canephora Asexual Propagation Vegetative Propagation Multiplication

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Nobiletin Prevents Body Weight Gain and Bone Loss in Ovariectomized C57BL/6j Mice

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Pharmacology & Pharmacy 2014年第10期5卷 959-965页

作者： Young-sil Lee Midori Asai Sun-sil Choi Takayuki Yonezawa Toshiaki Teruya Kazuo Nagai je-Tae Woo Byung-Yoon Cha Research Institute for Biological Functions Chubu University Kasugai Japan Faculty of Education University of the Ryukyus Nishihara Japan Department of Biological Chemistry Chubu University Kasugai Japan Department of Research and Development Erina Co. Inc. Tokyo Japan

Obesity and osteoporosis are associated with estrogen deficiency following menopause. Therefore, it is important to prevent and treat both disorders to maintain a healthy life in postmenopausal women. Nobiletin, a polymethoxylated flavone, exhibits various pharmacologic effects, including anti-tumor and anti-inflammatory activities. Therefore, in this study, we examined the effects of nobiletin on obesity, obesity-related metabolic disorders, and bone mass in ovariectomized (OVX) mice. Mice were divided into four groups and underwent sham operation or OVX. OVX mice were treated with 50 or 100 mg/kg nobiletin, or received vehicle alone (0.3% carboxyl methyl cellulose/0.5% dimethyl sulfoxide). Nobiletin decreased body weight gain and white adipose tissue weight in OVX mice. Nobiletin also decreased triglyceride levels, and tended to reduce plasma total cholesterol and glucose levels. Additionally, nobiletin prevented the reduction in bone mineral density of the trabecular region of the femur in OVX mice. Taken together, our results suggest that nobiletin improves adiposity, dyslipidemia, hyperglycemia, and prevents bone loss in OVX mice. Therefore, nobiletin is expected to have beneficial effects for the prevention and improvement of metabolic disorders and osteoporosis in postmenopausal women.

关键词： Nobiletin Ovariectomy Obesity Lipid and Glucose Metabolism Bone Mineral Density

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CHARACTERIZATION OF Si_(1-x-y)Ge_xC_y FILMS GROWN BY ION IMPLANTATION AND SUBSEQUENT SOLID PHASE EPITAXY

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Acta Metallurgica Sinica(English Letters) 2002年第1期15卷 49-52页

作者： X.Q. Liu Y.Y. Wang W.j. Cheng j. Zhang S.Y. Ma Department of Physics. Lanzhou University Department of physics Northwest Normal University

Si1-x-yGexCy ternary alloy films were grown on monocrystalline silicon substrates by C+ ion implantation and subsequent solid phase epitaxy (SPE). Two-step annealing technique was employed in the SPE. The structure and electrical properties of the alloy films were determined using Fourier transform infrared spectroscopy and van der Pauw Hall measurements, respectively. With the optimization of two-step annealing technique for the implanted Si1-x-yGexCy layers, a certain amount of C atoms occupied substitutional sites and no SiC was formed.

关键词： Si1-x-yGexCu film ion implantation SPE

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Entropy generation for mixed convection flow in vertical annulus with two regions hydromagnetic viscous and Cu-Ag water hybrid nanofluid through porous zone: a comparative numerical study

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Propulsion and Power Research 2022年第3期11卷 401-415页

作者： T.Rahim j.Hasnain N.Abid Z.Abbas Department of Mathematics The Islamia University of BahawalpurBahawalpur63100Pakistan Department of Computer Sciences Bahria UniversityIslamabad44000Pakistan

The primary determination of this study is a numerical investigation of the entropygeneration (EG) in the steady two-region flow of viscous fluid and hybrid nanofluid (NF) in along-infinite vertical annulus having a clear region as well as porous media. Stoke’s and single-phase NF models are used to study the viscous fluid and hybrid nanofluid (HNF) heat transferdevelopments, respectively. Two types of nanoparticles are taken, such as copper (Cu) and sil-ver (Ag) within base fluid water to make it a HNF. darcy-Brinkman law is also used to examinethe flow through the porous zone in the annulus. Necessary quantities have been used in thesystem of equations to transfer them into non-dimensional forms. For momentum and energytransport, the numerical results are evaluated for various model parameters and are examinedvia the shooting method in MATHEMATICA. It is noted that the momentum and energy trans-port are more significant when two immiscible fluids in a clear vertical annulus are taken. Thefindings also indicate that two-phase momentum and heat flow are greater when a NF is used in Region-II and lower when a HNF is used. The temperature (in Region-II) falls with a high na-nomaterials volume fraction (see Figure 4) while it is increased when the Hartman number isincreased. Moreover, velocity declines with increment in nanomaterials volume fraction. Thus,higher thermal conductivity can be accomplished by using a magnetic field.

关键词： Two-phase flow Hybrid nanofluid Viscous fluid Porous zone Vertical annulus Entropy generation

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低分子透明质酸片段对红细胞聚集诱发作用及其种属特异性

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青岛农业大学学报（自然科学版） 2022年第2期39卷 96-108页

作者：郭田田王家麒贾潇潇 LAZCANO sil VEIRA Rayko HUI SHOFARO j.ssica 王凤舞马泽芳惠觅宙青岛农业大学动物科技学院山东青岛266109

旨在开发一种组织渗透性好的35 kda透明质酸片段(HA35),探究HA35对红细胞的生物活性作用及其种属特异性。分别利用足量或轻度过量的重组人透明质酸酶PH20和牛提取透明质酸酶PH20,酶解切割平均分子量1600 kda的透明质酸原料,制备一种能通... 详细信息

旨在开发一种组织渗透性好的35 kda透明质酸片段(HA35),探究HA35对红细胞的生物活性作用及其种属特异性。分别利用足量或轻度过量的重组人透明质酸酶PH20和牛提取透明质酸酶PH20,酶解切割平均分子量1600 kda的透明质酸原料,制备一种能通过220 nm孔径的平均分子量35 kda的透明质酸片段HA35,并进一步使用HA35与人和不同动物的红细胞共同孵育,研究HA35对红细胞的生物活性作用及其种属特异性。结果表明:HA35和其他平均分子量不同的透明质酸片段,通过CD44受体诱发人和多种实验动物的红细胞呈钱串状聚集,但存在种属特异性。HA35和其他平均分子量不同的透明质酸片段,诱发红细胞钱串状聚集的透明质酸片段的最小浓度与其分子量呈负相关,这种负相关关系首次被用来准确测定低分子透明质酸片段的分子量。HA35不仅诱发人和动物红细胞钱串状聚集,还诱发红细胞沉降率增加,被用于HA35产品的生产质控。

关键词：低分子量透明质酸片段分子量测定红细胞钱串状聚集 CD44 红细胞沉降率

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利用固体类Fenton试剂降解五氯酚的探讨

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农业环境科学学报 2015年第10期34卷 1914-1920页

作者：杨冰 Pignatello joseph j 曲东西北农林科技大学资源环境学院陕西杨凌712100 Department of Environmental Sciences The Connecticut Agricultural Experiment Station

采用四种固体过氧化物(过氧化钙,CP;过硼酸钠,SPB;过碳酸钠,SPC;过氧化尿素,UHP)以及H2O2和两种铁源[(Fe3(PO4)2和Fe SO4],通过室内模拟试验,研究不同过氧化物和Fe(Ⅱ)源组合构成的(类)Fenton反应对石英砂中五氯酚(PCP)的降解能力,探讨... 详细信息

采用四种固体过氧化物(过氧化钙,CP;过硼酸钠,SPB;过碳酸钠,SPC;过氧化尿素,UHP)以及H2O2和两种铁源[(Fe3(PO4)2和Fe SO4],通过室内模拟试验,研究不同过氧化物和Fe(Ⅱ)源组合构成的(类)Fenton反应对石英砂中五氯酚(PCP)的降解能力,探讨了影响类Fenton试剂活性成分有效性和持续性的主要因素。结果表明,使用Fe SO4为铁源,添加CP、SPB或SPC使体系p H快速升高至10~12,极大抑制了铁源有效性,仅在反应初期对PCP有快速降解,24 h降解率分别为22.0%、14.6%和17.3%;添加H2O2或UHP对体系的p H无明显影响,24 h降解率为86.5%和83.8%;但在24~48 h,PCP降解均无明显增加,表明活性成分已殆尽。使用人工合成的纳米级Fe3(PO4)2为铁源,能够持续稳定地提供Fe(Ⅱ),在0~20 d实验期间PCP均有持续降解,在20 d时CP、SPB、SPC和UHP对PCP的降解率分别为30.1%、13.6%、8.6%、37.0%,H2O2对PCP在16 d时的降解率为55.6%。因此,使用致碱性过氧化物作为类Fenton试剂时,需要添加p H缓冲剂以提高对有机物的降解率;UHP在一定程度上可以替代H2O2。尽管固体类Fenton试剂对石英砂中PCP的去除率低于传统Fenton试剂,但活性成分持续时间能由不到24 h增加至20 d以上,故在土壤污染修复中具有更大潜力。

关键词：五氯酚化学降解固体过氧化物铁矿物芬顿反应

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Generation and Analysis of Hybrid-Electric Vehicle Transmission Shift Schedules with a Torque Split Algorithm

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journal of Transportation Technologies 2020年第1期10卷 21-49页

作者： Nicholas j. Connelly Derek I. George Andrew C. Nix W. Scott Wayne West Virginia University Morgantown WV USA

The increased concern over global climate change and lack of long-term sustainability of fossil fuels in the projected future has prompted further research into advanced alternative fuel vehicles to reduce vehicle emissions and fuel consumption. One of the primary advanced vehicle research areas involves electrification and hybridization of vehicles. As hybrid-electric vehicle technology has advanced, so has the need for more innovative control schemes for hybrid vehicles, including the development and optimization of hybrid powertrain transmission shift schedules. The hybrid shift schedule works in tandem with a cost function-based torque split algorithm that dynamically determines the optimal torque command for the electric motor and engine. The focus of this work is to develop and analyze the benefits and limitations of two different shift schedules for a position-3 (P3) parallel hybrid-electric vehicle. a traditional two-parameter shift schedule that operates as a function of vehicle accelerator position and vehicle speed (state of charge (SOC) independent shift schedule), and a three-parameter shift schedule that also adapts to fluctuations in the state of charge of the high voltage batteries (SOC dependent shift schedule). The shift schedules were generated using an exhaustive search coupled with a fitness function to evaluate all possible vehicle operating points. The generated shift schedules were then tested in the software-in-the-loop (sil) environment and the vehicle-in-the-loop (VIL) environment and compared to each other, as well as to the stock 8L45 8-speed transmission shift schedule. The results show that both generated shift schedules improved upon the stock transmission shift schedule used in the hybrid powertrain comparing component efficiency, vehicle efficiency, engine fuel economy, and vehicle fuel economy.

关键词： Hybrid-Electric Vehicles Torque Split Algorithm Shift Schedule Shift Map Cost Function SOC Dependent

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IFNbeta Induces secreted IL-1 receptor antagonist production through a MEK2/PI3Kdelta-dependent,ERK1/2-Independent pathway in human monocytes

IFNbeta Induces secreted IL-1 receptor antagonist production...

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21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology

作者： Karim j.Brandt Rakel Carpintero Lyssla Gruaz Nicolas Moinarfi danlelle Burger University of Geneva Switzerland University of California San FranciscoUSA

The secreted form of IL-1 receptor antagonist(sil-1Ra) is a natural inhibitor of the pro-inflammatory cytokine IL-1beta and therefore plays an important part in the regulation of inflammatory *** human monocytes,IFNbeta induces the production of slL-1Ra through the activation of PIsK but not of its canonical STAT *** used RNA interference and kinase

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SEDIMENT YIELD-SURFACE TOPOGRAPHY RELATIONSHIPS FOR SELECTED MISSISSIPPI SOILS

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International journal of Sediment Research 2000年第1期15卷 1-16页

作者： M. j. M. ■(USda-ARS National Sedimentation Laboratory, Oxford, MS 38655)K. Helming (ZALF, Muencheberg, Germany)S. N. Prasad(Dept. of Civil Engineering, University of Mississippi, University, MS 38677

Microtopography is an important surface characteristic of upland areas that affect erosion processes of detachment, transport, and runoff Yet, little quantitative information is available about the relationship between surface microtopography and sediment yield. A laboraory rainfall simulation study was conducted to determine for four different erosion susceptible soils the changes in surface microtopography and sediment yield during a series of 6 to 8 rainstorms of 0.75 h duration and 60 mm h-1 intensity each. The soils chosen were the Ap-materials of a Grenada sil (Glossic Fragiudalfs), Atwood sil (Typic Paleudalfs), and a Forestdale sicl (Typic Ochraqualfs), as well as the C-material, a Glauconitic sediment, of a Ruston sil (Typic Paleudalfs). Soil beds were prepared in a flume with a seedbed-like surface condition. Before all and after each rainstorm, the surface microtopography was determined using a laser microreliefmeter. Microtopography, expressed in terms of the mean local topographic gradient, and runoff data indicate a very similar pattern among the four soils. The data show an initially rapid increase in the sediment concenttation, which reached quickly a maximum and then gradually decreased to a near constant value at the end of the storm series. Sediment yield followed closely the sediment concentration trend due to a near constant runoff rate. Surface microtopography changed rapidly during the first rainstorm but then decreased more gradual to an approximate constant value for most of the later rainstorms in the sequence. Three distinct phases in the sediment yieldmicrotopography relationship are recognized: (1) a preponding phase, (2) a post ponding-increased sediment yield phase, and (3) a post ponding-decreased sediment yield phase. These phases reflect changes in the relative importance of soil erosion processes of roughness dissipation, rill development,and soil surface matrix stabilization.

关键词： Surface microrelief sediment yield sediment concentration surface roughness microtopography runoff

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