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Particuology 2015年第3期13卷 114-123页

作者： M.Domat F.E.Kruis N.L.Azong-Wara j.M.Fernandez-diaz Department of Physics University of OviedoC/Calvo Sotelos/nE-33007 OviedoSpain Institute of Technology for Nanostructures(NST) and Center for Nanointegration Duisburg-Essen(CENIDE) University of Duisburg-EssenBismarckstr.81D-47057 DuisburgGermany

The inversion of the particle size distribution from electrical mobility measurements is analyzed. Three different methods are adapted for a dot-matrix approach to the problem, especially for non-square or singular matrices, and applied to electrical mobility measurements from fixed or scanning voltages. Mul- tiply charged particles, diffusion losses, arbitrary voltage steps and noise were considered, which results in non-adjoining and overlapping transfer functions. The individual contribution of the transfer func- tions in each size interval was geometrically estimated, which requires only its characteristic mobilities. The methodology is applied to mobility measurements from particles charged with unipolar and bipolar chargers. However, the method can be extrapolated to any charging method with a defined charge distribution, and retrieval of the singly charged particle distribution and mean charge from a tandem differential mobility analysis configuration was successfully demonstrated.

关键词： Tandem differential mobility analysisInverse problemRegularization methodsSize distributionTransfer functionUnipolar charger

Altitudinal differentiation of Quercus robur in Bosnia and Herzegovina

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journal of Forestry Research 2018年第5期29卷 1225-1232页

作者： D.Ballian M.Memievi F.Boguni I.j.diaz-Maroto Faculty of Forestry University of Sarajevo Higher Polytechnic School of Engineering University of Santiago de CompostelaCampus Universitario

Morphological variation based on eight measured and four derived traits was studied to establish whether there was significant variation between populations and to identify the influence of the altitude on morphological differentiation among 44 natural stands of common oak（Quercus robur L.） in Bosnia and Herzegovina,ranging from 82 to 860 *** results point to significant intra-and inter-population ***-related variation is less pronounced and determined largely by microclimatic *** results could provide management strategies for species reintroduction in the study *** are no differences in leaf morphology between the different branches of individual ***,there are important variations between the altitudinal groups and closely linked to environmental factors in all *** important recommendation is to use seeds from oak with attention to their altitudinal origin.

关键词： Common oak Conservation Ecology Management Morphology Renewal

Single molecular DNA/RNA sequencing with microscopy

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Asian journal of Pharmaceutical Sciences 2016年第1期11卷 83-84页

作者： Masanori Kataoka Kuniaki Nagayama Hidehiro oana Oceanography Section Science Research CenterKochi UniversityKochiJapan The Graduate University for Advanced Studies KanagawaJapan Department of Mechanical Engineering The University of TokyoTokyoJapan

Ultra high-throughput DNA sequencing has been a very hot topic beyond the field of genomic *** have been various approaches to this issue ranging from direct observation of individual DNA synthesis[1],amperometric/optical detection of bases using a nanopore[2,3]and direct sequencing with a high-resolution probe microscope[4].Recently,we have developed a technique to chemically modify all nucleobases in DNA[5],so that the bases A,T,G,C can be differentiated in the sequence by using high-resolution electron microscopy(EM).

关键词： Single molecular DNA/RNA sequencing Quantum dot complex

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

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Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal j.Goldschmidt-Clermont Aisha Khan George jimsheleishvili Patricia Graham Adriana Brooks Risset Silvera Alexander j.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi james D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

Ballistic impact response of flexible and rigid UHMWPE textile composites:Experiments and simulations

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Defence Technology（防务技术） 2023年第4期22卷 37-53页

作者： Hongxu Wang Dakshitha Weerasinghe Paul j.Hazell Damith Mohotti Evgeny V.Morozov juan P.Escobedo-diaz School of Engineering and Information Technology The University of New South WalesCanberraACT2600Australia

This study elaborates on the effects of matrix rigidity on the high-velocity impact behaviour of UHMWPE textile composites using experimental and numerical *** composite samples were manufactured of a plain-weave fabric(comprising Spectra?1000 fibres)and four different matrix ***-velocity impact tests were conducted by launching a spherical steel projectile to strike on the prepared samples via a gas *** experimental results showed that the textile composites gradually changed from a membrane stretching mode to a plate bending mode as the matrix rigidity and thickness *** composites deformed in the membrane stretching mode had higher impact resistance and energy absorption capacity,and it was found that the average energy absorption per ply was much higher in this mode,although the number of broken yarns was smaller in the perforated ***,the flexible matrix composites always had higher perforation resistance but larger deformation than the rigid matrix counterparts in the tested thickness and velocity range.A novel numerical modelling approach with enhanced computational efficiency was proposed to simulate textile composites in mesoscale *** simulation results revealed that stress and strain development in the more rigid matrix composite was localised in the vicinity of the impact location,leading to larger local deformation and inferior perforation resistance.

关键词： Impact behaviour Textile composite UHMWPE fibre Energy absorption Finite element analysis

Infuence of elemental composition in environmental impacts of steel

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journal of Iron and Steel Research International 2020年第5期27卷 598-607页

作者： E.Batuecas C.Mayo R.diaz F.j.Perez Thermal and Fluid Engineering Department Carlos III University of Madrid28911 LeganesMadridSpain Chemical and Materials Engineering Department Complutense University of Madrid28040 MadridSpain Technical Sciences and Engineering UDIMA University28400 Collado VillalbaMadridSpain。

The environmental behavior of four steels was *** the operation phase of concentrating solar power plants,steels withstand high temperature because of its contact with molten ***,choosing the steel type for the molten salt tanks remains a great *** the cold tank,carbon steel is usually used although an approach with low chromium content steel is being studied for these ***,in high temperature applications,such as hot store tank,austenitic stainless steel is the most frequent ***,ferritic steel is being considered as a promising material in these *** many researchers studied the steel technical properties without considering their environmental damages,this work aimed to introduce the environmental aspects into the material choice by using the life cycle assessment *** one hand,the results showed the environmental adequacy of carbon steel against low chromium content *** the other hand,the results obtained in those steels suitable in high temperature application revealed signifcant environmental benefts from the ferritic steel instead of the austenitic steel.

关键词： Renewable energy Concentrating solar power Life cycle assessment Molten salt Ferritic steel Austenitic steel

Redox Regulation of Glycogen Biosynthesis in the Cyanobacterium Synechocystis sp. PCC 6803： Analysis of the AGP and Glycogen Synthases

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Molecular Plant 2014年第1期7卷 87-100页

作者： Sandra diaz-Troya Luis Lopez-Maury Ana Maria Sanchez-Riego Miguel Roldan Francisco j. Florencio Instituto de Bioquimica Vegetal y Fotosintesis Universidad de Sevilla-CSIC Americo Vespucio 49 E-41092 Sevilla Spain

Glycogen constitutes the major carbon storage source in cyanobacteria, as starch in algae and higher plants. Glycogen and starch synthesis is linked to active photosynthesis and both of them are degraded to glucose in the dark to maintain cell metabolism. Control of glycogen biosynthesis in cyanobacteria could be mediated by the regulation of the enzymes involved in this process, ADP-glucose pyrophosphorylase （AGP） and glycogen synthase, which were identified as putative thioredoxin targets. We have analyzed whether both enzymes were subjected to redox modification using purified recombinant enzymes or cell extracts in the model cyanobacterium Synechocystis sp. PCC 6803. Our results indicate that both AGP and glycogen synthases are sensitive to copper oxidation. However, only AGP exhibits a decrease in its enzymatic activity, which is recovered after reduction by DTT or reduced thioredoxin （TrxA）, suggesting a redox control of AGP. In order to elucidate the role in redox control of the cysteine residues present on the AGP sequence （C45, C185, C320, and C337）, they were replaced with serine. All AGP mutant proteins remained active when expressed in Synechocystis, although they showed different electrophoretic mobility profiles after copper oxidation, reflecting a complex pattern of cysteines interaction.

关键词： cyanobacteria glycogen Synechocystis thioredoxin redox regulation.

Design,synthesis and performance of Ba-doped derivatives of SrMo_(0.9)Fe_(0.1)O_(3-δ)perovskite as anode materials in SOFCs

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journal of Materiomics 2019年第2期5卷 280-285页

作者： S.Sydyknazar V.Cascos M.T.Fernandez-diaz j.A.Alonso Instituto de Ciencia de Materiales de Madrid C.S.I.C.CantoblancoE-28049MadridSpain Institut Laue Langevin BP 156XGrenobleF-38042France

The oxides SrMo_(1-x)Fe_(x)O_(3-δ)(x=0.1 and 0.2)have recently been described as excellent anode materials for solid-oxide fuel cells at intermediate temperatures(IT-SOFC)with LSGM as the *** this work we have partially replaced Sr by Ba in a compound of formula Sr_(0.9)Ba_(0.1)Mo_(0.9)Fe_(0.1)O_(3-δ),in order to expand the unit-cell size and thereby improve the ionic diffusion of O^(2-)ions through the crystal *** anode materials must be stable under reducing conditions,since they work in the presence of the fuel(H_(2))at elevated *** anodes are inspired in the SrMoO_(3)perovskite,which contains Mo4+ions,stable in very reducing *** novel oxide has been structurally characterized from x-ray(XRD)and neutron powder diffraction(NPD)data;the structure is defined at room temperature in the Pm-3m space group,and shows oxygen vacancies,necessary for the performance of this material as mixed ionic-electronic(MIEC)*** single test cells supported on LSGM,the materials generated output powers close to 500 mW/cm^(2)at 850℃using pure H_(2)as *** thermal expansion is linear,with TEC=10.93×10^(-6)K^(-1).The chemical compatibility with the LSGM electrolyte was also verified.

关键词： IT-SOFC Anode Perovskite Neutron diffraction SrMoO_(3)

Comparative transcriptomics reveals hidden issues in the plant response to arthropod herbivores

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journal of Integrative Plant Biology 2021年第2期63卷 312-326页

作者： M.Estrella Santamaria Alejandro Garcia Ana Amaiz Irene Rosa-diaz Gara Romero-Hernandez Isabel diaz Manuel Martinez Centro de Biotecnologia y Genomica de Plantas Instituto Nacional de Investigacion y Tecnologia Agraria y AlimentariaUniversidad Politecnica de MadridMadridSpain Departamento de Biotecnologia-Biologia Vegetal Escuela Tecnica Superior de Ingenierfa AgronomicaAlimentaria y de BiosistemasUniversidad Politecnica de MadridMadridSpain

Plants experience different abiotic/biotic stresses,which trigger their molecular machinery to cope with them. Besides general mechanisms prompted by many stresses, specific mechanisms have been introduced to optimize the response to individual threats. However, these key mechanisms are difficult to identify. Here, we introduce an in-depth species-specific transcriptomic analysis and conduct an extensive meta-analysis of the responses to related species to gain more knowledge about plant responses. The spider mite Tetranychus urticae was used as the individual species, several arthropod herbivores as the related species for meta-analysis, and Arabidopsis thaliana plants as the common host. The analysis of the transcriptomic data showed typical common responses to herbivory, such as jasmonate signaling or glucosinolate biosynthesis. Also, a specific set of genes likely involved in the particularities of the Arabidopsis-spider mite interaction was discovered. The new findings have determined a prominent role in this interaction of the jasmonateinduced pathways leading to the biosynthesis of anthocyanins and tocopherols. Therefore, tandem individual/general transcriptomic profiling has been revealed as an effective method to identify novel relevant processes and specificities in the plant response to environmental stresses.

关键词： Arabidopsis thaliana arthropod herbivore comparative transcriptomics plant defense Tetranychus urticae

DBD-Corona Discharge for Degradation of Toxic Gases

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Plasma Science and Technology 2007年第6期9卷 682-685页

作者： M.PACHECO-PACHECO j.PACHECO-SOTELO H.MORENO-SAAVEDRA j.A.diaz-GOMEZ A.MERCADO-CABRERA M.YOUSFI National Institute of Nuclear Research OcoyoacacMexico Toluca Institute of Technology TolucaMexico Center of Plasma Physics and their Applications of Toulouse France

The non-thermal plasma technology is a promising technique to treat SO2 and NOx. Chemical radicals produced with this technology can remove several pollutants at atmospheric pressure in a very short period of time simultaneously. Both theoretical and experimental study on SO2 and NOx removal, by a dielectric barrier discharge （DBD） with corona effect, is presented.

关键词： DBD-corona de-NOx process de-SO2 process