Single molecular DNA/RNA sequencing with microscopy

作     者：Masanori Kataoka Kuniaki Nagayama Hidehiro Oana 

作者机构：Oceanography SectionScience Research CenterKochi UniversityKochiJapan The Graduate University for Advanced StudiesKanagawaJapan Department of Mechanical EngineeringThe University of TokyoTokyoJapan 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2016年第11卷第1期

页      面：83-84页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：Japan Society for the Promotion of Science  JSPS  (15K12757) 

主　　题：Single molecular DNA/RNA sequencing Quantum dot complex 

摘      要：Ultra high-throughput DNA sequencing has been a very hot topic beyond the field of genomic *** have been various approaches to this issue ranging from direct observation of individual DNA synthesis[1],amperometric/optical detection of bases using a nanopore[2,3]and direct sequencing with a high-resolution probe microscope[4].Recently,we have developed a technique to chemically modify all nucleobases in DNA[5],so that the bases A,T,G,C can be differentiated in the sequence by using high-resolution electron microscopy(EM).