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Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PnO1 Expression and Activating p53/p21 Signaling Pathway

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Chinese journal of Integrative Medicine 2024年第6期30卷 515-524页

作者： CAO Liu-j.ng LIU Li-ya CHEn You-qin HAn Yu-ying WEI Li-hui YAO Meng-ying FAnG Yi WU Mei-zhu CHEnG Ying Thomas j.Sferra LIU Hui-xin LI Li peng j.n SHEn A-ling Clinical Research Institute the Second Affiliated Hospital&Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou350122China Fujian Key Laboratory of Integrative Medicine in Geriatrics Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Pediatrics Case Western Reserve University School of MedicineUH Rainbow Babies and Children’s HospitalClevelandOH44106USA Innovation and Transformation Center Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Health Management Fujian Provincial HospitalShengli Clinical College of Fujian Medical UniversityFuzhou000000China

ObjectiveTo explore the regulatory effect of Pien Tze Huang (PZH) on targeting partner of nOB1 (PnO1) and it’s down-stream mediators in colorectal cancer (CRC) *** polymerase chain reaction was performed to determine mRnA levels of PnO1, TP53, and CDKn1A. Western blotting was performed to determine protein levels of PnO1, p53, and p21. HCT-8 cells were transduced with a lentivirus over-expressing PnO1. Colony formation assay was used to detect cell survival in PnO1 overexpression of HCT-8 cells after PZH treatment. Cell-cycle distribution, cell viability and cell apoptosis were performed to identify the effect of PnO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment. Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PnO1 or sh-Ctrl lentivirus were evaluated. Western blot assay was performed to detect PnO1, p53, p21 and PCnA expression in tumor sections. Terminal deoxynucleotidyl transferase dUTP nick end labling (TUnEL) assay was used to determine the apoptotic cells in *** treatment decreased cell viability, down-regulated PnO1 expression, and up-regulated p53 and p21 expressions in HCT-8 cells (PnO1 overexpression attenuated the effects of PZH treatment, including the expression of p53 and p21, cell growth, cell viability, cell cycle arrest and cell apoptosis in vitro (PnO1 knockdown eliminated the effects of PZH treatment on tumor growth, inhibiting cell proliferation inhibition and apoptosis induction in vivo (PnO1 knockdown attenuated the effects of PZH treatment on the down-regulation of PnO1 and up-regulation of p53 and p21 in vivo (PnclusionThe mechanism by which PZH induces its CRC anti-proliferative effect is at least in part by regulating the expression of PnO1 and its downstream targets p53 and p21.

关键词： colorectal cancer Pien Tze Huang Chinese medicine partner of nOB1 proliferation p53/p21 pathway

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The DnA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

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journal of Integrative Agriculture 2024年第6期23卷 1956-1966页

作者： Yue jiang Rong Wang Lili Du Xueyu Wang Xi Zhang pengfei Qi Qianfei Wu Baoyi peng Zonghua Wang Mo Wang Ya Li State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultural UniversityKunming 650201China Fujian Universities Key Laboratory for Plant-Microbe Interaction Fujian Agriculture and Forestry UniversityFuzhou 350002China Ministerial and Provincial joint Innovation Centre for Safety Production of Cross-Strait Crops Minjiang UniversityFuzhou 350108China Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests Fuzhou 350003China

The conserved DnA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and ***,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains *** this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in *** first demonstrated the importance of DnA damage repair in *** by showing that the DnA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this ***,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in *** deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in ***,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DnA damage ***,our results provide insights into the roles of MoMMS21 and MoSMC5 in ***,highlighting their functions beyond DnA damage repair.

关键词： Magnaporthe oryzae MMS21 SMC5 DnA damage repair pathogenicity

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Genome-wide association study reveals that jASMOnATE ZIM-DOMAIn 5 regulates seed germination in rice

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The Crop journal 2024年第4期12卷 1001-1009页

作者： Shan Sun Liling peng Qianqian Huang Zhibo Huang Chengj.ng Wang jia Zhao Zhoufei Wang Yongqi He The Laboratory of Seed Science and Technology Guangdong Key Laboratory of Plant Molecular BreedingKey Laboratory for Enhancing Resource Use Efficiency of Crops in South ChinaMinistry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou 510642GuangdongChina

Seed germination is a complex trait regulated by multiple genes in ***,the regulators of rice seed germination have yet to be sufficiently ***,a quantitative trait locus(QTL)for rice seed germination was identified in a genome-wide association *** candidate gene jASMOnATE ZIM-DOMAIn 5(OsjAZ5)of the QTL was verified that positively regulates seed ***5 regulation of seed germination involves an OsABI3-mediated abscisic acid *** of OsjAZ5 facilitated seed *** application of OsjAZ5 might be useful for increasing seed germination for rice direct seeding.

关键词： Abscisic acid jASMOnATE ZIM-DOMAIn Oryza sativa Seed germination

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Grain boundary engineering for enhancing intergranular damage resistance of ferritic/martensitic steel P92

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nuclear Science and Techniques 2024年第5期35卷 186-199页

作者： Lei peng Shang-Ming Chen j.ng-Yi Shi Yong-jie Sun Yi-Fei Liu Yin-Zhong Shen Hong-Ya He Hui-juan Wang jie Tian State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China School of nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China School of Materials Engineering Shanghai University of Engineering Science333 Long Teng RoadShanghai 201620China Experimental Center of Engineering and Material Science University of Science and Technology of ChinaHefei 230026China

Ferritic/martensitic(F/M)steel is widely used as a structural material in thermal and nuclear power ***,it is susceptible to intergranular damage,which is a critical issue,under service *** this study,to improve the resistance to intergranular damage of F/M steel,a thermomechanical process(TMP)was employed to achieve a grain boundary engineering(GBE)microstructure in F/M steel *** TMP,including cold-rolling thickness reduction of 6%,9%,and 12%,followed by austenitization at 1323 K for 40 min and tempering at 1053 K for 45 min,was applied to the as-received(AR)P92 *** prior austenite grain(PAG)size,prior austenite grain boundary character distribution(GBCD),and connectivity of prior austenite grain boundaries(PAGBs)were *** to the AR specimen,the PAG size did not change *** fraction of coincident site lattice boundaries(CSLBs,3≤Σ≤29)and Σ3^(n) boundaries along PAGBs decreased with increasing reduction ratio because the recrystallization fraction increased with increasing reduction *** PAGB connectivity of the 6%deformed specimen slightly deteriorated compared with that of the AR ***,potentiodynamic polarization studies revealed that the intergranular damage resistance of the studied steel could be improved by increasing the fraction of CSLBs along the PAGBs,indicating that the TMP,which involves low deformation,could enhance the intergranular damage resistance.

关键词： Grain boundary engineering Ferritic/martensitic steel Prior austenite grain boundary character distribution Grain boundary connectivity Intergranular damage resistance

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Dynamic immune recovery process after liver transplantation revealed by single-cell multi-omics analysis

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The Innovation 2024年第3期5卷 55-68页

作者： Rui Wang Xiao peng Yixin Yuan Baojie Shi Yuan Liu Hengxiao ni Wenzhi Guo Qiwei Yang Pingguo Liu jie Wang Zhaojie Su Shengnan Yu Dehua Liu j.nyan Zhang j.njie Xia Xueni Liu Hao Li Zhengfeng Yang Zhihai peng Organ Transplantation Clinical Medical Center of Xiamen University Department of General SurgeryXiang’an Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361102China Organ Transplantation Institute of Xiamen University Xiamen Human Organ Transplantation Quality Control CenterXiamen Key Laboratory of Regeneration MedicineFujian Provincial Key Laboratory of Organ and Tissue RegenerationSchool of MedicineXiamen UniversityXiamen 361102China Department of General Surgery Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China State Key Laboratory of Cellular Stress Biology School of Life SciencesFaculty of Medicine and Life SciencesXiamen UniversityXiamen 361102China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China Department of Hepatobiliary&Pancreatic Surgery The National Key Clinical SpecialtyZhongshan Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361102China Precision Research Center for Refractory Diseases Institute for Clinical ResearchShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 201620China

Elucidating the temporal process of immune remodeling under immunosuppressive treatment after liver transplantation(LT)is critical for precise clinical management ***,we performed a single-cell multi-omics analysis of peripheral blood mononuclear cells(PBMCs)collected from LT patients(with and without acute cellular rejection[ACR])at 13 time *** was performed in two independent cohorts with additional LT patients and healthy *** study revealed a four-phase recovery process after LT and delineated changes in immune cell composition,expression programs,and interactions along this *** intensity of the immune response differs between the ACR and non-ACR ***,the newly identified inflamed nK cells,CD14+RnASE2+monocytes,and FOS-expressing monocytes emerged as predictive indicators of *** study illuminates the longitudinal evolution of the immune cell landscape under tacrolimus-based immunosuppressive treatment during LT recovery,providing a four-phase framework that aids the clinical management of LT patients.

关键词： process analysis precise

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Hemodialysis bilayer bionic blood vessels developed by the mechanical stimulation of hepatitis B viral X(HBX)genetransfected hepatic stellate cells

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journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第6期25卷 499-512页

作者： Hongyi LIU Yuanyuan ZHOU peng GUO Xiongwei ZHEnG Weibin CHEn Shichao ZHAnG Yu FU Xu ZHOU Zheng WAn Bin ZHAO Yilin ZHAO Department of Oncology and Vascular Interventional Radiology Zhongshan Hospital Affiliated to Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361004China School of Medicine Xiamen UniversityXiamen 361102China Xiamen Health and Medical Big Data Center Xiamen 361008China Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma(Zhongshan Hospital Affiliated to Xiamen University) Xiamen 361004China Xiamen Key Laboratory of Cellular Intervention and Interventional Medical Materials Xiamen 361004China

Artificial vascular graft(AVG)fistula is widely used for hemodialysis treatment in patients with renal ***,it has poor elasticity and compliance,leading to stenosis and *** ideal artificial blood vessel for dialysis should replicate the structure and components of a real artery,which is primarily maintained by collagen in the extracellular matrix(ECM)of arterial *** have revealed that in hepatitis B virus(HBV)-induced liver fibrosis,hepatic stellate cells(HSCs)become hyperactive and produce excessive ECM ***,mechanical stimulation can encourage ECM secretion and remodeling of a fiber *** on the above factors,we transfected HSCs with the hepatitis B viral X(HBX)gene for simulating the process of HBV ***,these HBX-HSCs were implanted into a polycaprolactonepolyurethane(PCL-PU)bilayer scaffold in which the inner layer is dense and the outer layer consists of pores,which was mechanically stimulated to promote the secretion of collagen nanofiber from the HBX-HSCs and to facilitate crosslinking with the *** obtained an ECM-PCL-PU composite bionic blood vessel that could act as access for dialysis after ***,the vessel scaffold was implanted into a rabbit’s neck arteriovenous fistula *** exhibited strong tensile strength and smooth blood flow and formed autologous blood vessels in the rabbit’s *** study demonstrates the use of human cells to create biomimetic dialysis blood vessels,providing a novel approach for creating clinical vascular access for dialysis.

关键词： Composite bilayer bionic blood vessel Extracellular matrix(ECM) Hepatic stellate cells(HSCs) Hepatitis B viral X(HBX)gene Mechanical force

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Metal-free two-dimensional phosphorene-based electrocatalyst with covalent P-n heterointerfacial reconstruction for electrolyte-lean lithium-sulfur batteries

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Carbon Energy 2024年第5期6卷 175-185页

作者： jiangqi Zhou Chengyong Shu jiawu Cui Chengxin peng Yong Liu Weibo Hua Laura Simonelli Yuping Wu Shi Xue Dou Wei Tang School of Chemical Engineering and Technology Xi'an Jiaotong UniversityXi'anChina School of Materials Science and Engineering University of Shanghai for Science and TechnologyShanghaiChina State Key Laboratory of Space Power-Sources Technology Shanghai Institute of Space Power-SourcesShanghaiChina CELLS-ALBA Synchrotron BarcelonaSpain School of Energy and Environment Southeast UniversityNanjingChina

The use of lithium-sulfur batteries under high sulfur loading and low electrolyte concentrations is severely restricted by the detrimental shuttling behavior of polysulfides and the sluggish kinetics in redox ***-dimensional(2D)few layered black phosphorus with fully exposed atoms and high sulfur affinity can be potential lithium-sulfur battery electrocatalysts,which,however,have limitations of restricted catalytic activity and poor electrochemical/chemical *** resolve these issues,we developed a multifunctional metal-free catalyst by covalently bonding few layered black phosphorus nanosheets with nitrogen-doped carbon-coated multiwalled carbon nanotubes(denoted c-FBP-nC).The experimental characterizations and theoretical calculations show that the formed polarized P-n covalent bonds in c-FBP-nC can efficiently regulate electron transfer from nC to FBP and significantly promote the capture and catalysis of lithium polysulfides,thus alleviating the shuttle ***,the robust 1D-2D interwoven structure with large surface area and high porosity allows strong physical confinement and fast mass ***,with c-FBP-nC as the sulfur host,the battery shows a high areal capacity of 7.69 mAh cm^(−2) under high sulfur loading of 8.74 mg cm^(−2) and a low electrolyte/sulfur ratio of 5.7μL mg^(−1).Moreover,the assembled pouch cell with sulfur loading of 4 mg cm^(−2) and an electrolyte/sulfur ratio of 3.5μL mg^(−1) shows good rate capability and outstanding *** work proposes an interfacial and electronic structure engineering strategy for fast and durable sulfur electrochemistry,demonstrating great potential in lithium-sulfur batteries.

关键词： black phosphorus electronic structure high sulfur loading interfacial covalent bonds lean electrolyte

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基于PEI界面作用增强的AP热分解催化性能

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火炸药学报 2024年第3期47卷 262-270,I0006页

作者：许洪鹏黄琪金波彭汝芳西南科技大学环境友好能源材料国家重点实验室四川绵阳621010 西南科技大学材料与化学学院四川绵阳621010

为了改善高氯酸铵(AP)的热分解性能,基于聚乙烯亚胺(PEI)本征的正电荷和氨基基团,在与负电荷AP发生静电自组装包覆的同时,吸附铜金属催化剂于AP表面,制备了AP/PEI/Cu(ClO_(4))_(2)包覆样品,并通过DTA和TG-DSC等热分析技术研究了其热分... 详细信息

为了改善高氯酸铵(AP)的热分解性能,基于聚乙烯亚胺(PEI)本征的正电荷和氨基基团,在与负电荷AP发生静电自组装包覆的同时,吸附铜金属催化剂于AP表面,制备了AP/PEI/Cu(ClO_(4))_(2)包覆样品,并通过DTA和TG-DSC等热分析技术研究了其热分解行为;通过SEM、XPS、XRD、IR、ICP-OES和Zeta电位等测试手段对AP/PEI/Cu(ClO_(4))_(2)包覆样品的形貌与结构进行了表征。结果表明,与原料AP相比,AP/PEI/Cu(ClO_(4))_(2)包覆样品的高温分解峰温降低了94.2℃,表观放热量增加了512.91 j/g,证实PEI/Cu(ClO_(4))_(2)对AP的热分解具有良好的催化效果;PEI的加入能增强金属催化剂和AP的界面作用,使得催化剂更均匀地分布于AP表面,从而促使金属催化剂更好地发挥热分解催化作用。

关键词：物理化学高氯酸铵 AP 聚乙烯亚胺 PEI 催化热分解静电自组装

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natural variation in the cytochrome c oxidase subunit 5B OsCOX5B regulates seed vigor by altering energy production in rice

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journal of Integrative Agriculture 2024年第9期23卷 2898-2910页

作者： Chengwei Huang Zhijuan ji Qianqian Huang Liling peng Wenwen Li Dandan Wang Zepeng Wu jia Zhao Yongqi He Zhoufei Wang Laboratory of Seed Science and Technology/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/South China Agricultural University Guangzhou 510642China national nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572025 China national Rice Research Institute Hangzhou 310006China

Seed vigor is a crucial trait for the direct seeding of *** we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study *** major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and *** candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/*** disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle *** glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed *** elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed ***,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding.

关键词： cytochrome c oxidase natural variation rice seed vigor

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new perspectives on the genetic structure of dotted gizzard shad(Konosirus punctatus)based on RAD-seq

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Marine Life Science & Technology 2024年第1期6卷 50-67页

作者： Ying peng Yifan Liu jiasheng Li Kun Zhang Xun j.n Sixu Zheng Yunpeng Wang Zhenming Lu Liqin Liu Li Gong Bingjian Liu national Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization Zhejiang Ocean UniversityZhoushan 316022China national Engineering Research Center for Facilitated Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean UniversityZhoushan 316022China

To maintain,develop and rationally utilize marine organisms,understanding their genetic structure and habitat adaptation pattern is *** punctatus,which is a commercial fish species inhabiting the Indo-west Pacific Ocean,has shown an obvious annual global capture and aquaculture production decline due to climate changes and human *** the present study,restriction-site associated DnA sequencing(RAD-seq)was used to describe its genome-wide single nucleotide polymorphisms panel(SnPs).Among 146 individuals collected at nine locations scattered in China,Korea and japan,a set of 632,090 SnPs were *** genetic analysis showed that *** individuals were divided into two significant genetic ***,potential genetic differentiation between northern and southern population of *** was *** results indicated that gene flow existed among sampling locations of ***,especially from southern japan to ***,candidate genes associated with habitat adaptations of *** were identified,which are involved in diverse physiological processes of *** including growth and development(e.g.,KIDInS220,PAn3),substance metabolism(e.g.,PGM5)and immune response(e.g.,VAV3,CCT7,HSPA12B).Our findings may aid in understanding the possible mechanisms for the population genetic structure and local adaptation of ***,which is beneficial to establish the management and conservation units of ***,guiding the rational use of resources,with reference significance for a profound understanding of the adaptative mechanisms of other marine organisms to the environment.

关键词： Population structure Konosirus punctatus SnP RAD-seq Local adaptation

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