Natural variation in the cytochrome c oxidase subunit 5B OsCOX5B regulates seed vigor by altering energy production in rice

作     者：Chengwei Huang Zhijuan Ji Qianqian Huang Liling Peng Wenwen Li Dandan Wang Zepeng Wu Jia Zhao Yongqi He Zhoufei Wang Chengwei Huang;Zhijuan Ji;Qianqian Huang;Liling Peng;Wenwen Li;Dandan Wang;Zepeng Wu;Jia Zhao;Yongqi He;Zhoufei Wang

作者机构：Laboratory of Seed Science and Technology/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/South China Agricultural UniversityGuangzhou 510642China National Nanfan Research Institute(Sanya)Chinese Academy of Agricultural SciencesSanya 572025 China National Rice Research InstituteHangzhou 310006China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第9期

页      面：2898-2910页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the Hainan Province Science and Technology Special Fund,China(ZDYF2023XDNY086) the Project of Sanya Yazhou Bay Science and Technology City,China(SCKJ-JYRC-2022-87) the Natural Science Foundation of Guangdong Province,China(2023A1515012052 and 2023A1515012092) the Guangzhou Science and Technology Plan Project,China(2023A04J1452 and 2023A04J0749) the Double First-class Discipline Promotion Project,China(2021B10564001) 

主　　题：cytochrome c oxidase natural variation rice seed vigor 

摘      要：Seed vigor is a crucial trait for the direct seeding of *** we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study *** major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and *** candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/*** disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica Nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle *** glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed *** elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed ***,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding.