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Portomesenteric Venous Thrombosis in Patients after Laparoscopic Bariatric Surgery

Surgical Science 2022年第9期13卷 419-428页

作者： Augusto Tinoco Renam C. Tinoco Luciana El-Kadre Matheus P. S. Netto j.lia M. Rocha Diogo R. de Souza Douglas G. Tavares Department of Surgery S&#227o Jos&#233 do Ava&#237 Hospital Itaperuna RJ Brazil São josé do Avaí Hospital Itaperuna RJ Brazil

Objective: Obesity has become a worldwide epidemic that is directly related to chronic tissue inflammation and impaired fibrinolysis, both of which lead to an increased risk of thrombosis. The management of complications after bariatric surgery is still a challenge. The study aims to report the incidence of portomesenteric venous thrombosis (PMVT) in patients undergoing bariatric surgery. Introduction: Bariatric surgery is the procedure of choice for the treatment of obesity type III. Despite the safe technique, the surgeon must be aware of the risks linked to laparoscopy such as the PMVT. Computed tomography (CT) with intravenous contrast was the imaging method of choice for diagnosis. Materials and Methods: We performed a retrospective analysis of 4790 patients from August 1999 to june 2020 was observed the incidence of PMVT in this group. Of all patients, 72.8% were female and 27.2% male. The mean age was 46.3 years (34 - 72) and the mean BMI before bariatric surgery was 41.4 kg/m2. Results: The study showed an incidence of PMVT of 0.12% (N = 6), portal vein thrombosis was of 0.10% (n = 5) and 0.03% of MVT (n = 1). Of these, 72.5% (n = 3473 patients) underwent laparoscopic gastric bypass, 23.6% (n = 1131 patients) were submitted to the laparoscopic sleeve gastrectomy and 3.8% (n = 186 patients) to other procedures. The mean postoperative hospital stay for bariatric surgery was 3.3 days. The average time of hospital stay of patients complicated with PMVT range from 5 to 14 days. No patient died of complications related to PMVT. Conclusion: Portomesenteric vein thrombosis is a rare presentation after laparoscopic bariatric surgery. However, it is a serious complication that has a high index of suspicion for making its diagnosis. Conservative treatment using anticoagulants and thrombolytics is effective, it should always be considered the main treatment option. However, these patients must be followed up in the long term, as late complications can occur i

关键词： Morbid Obesity Laparoscopy Portomesenteric Venous Thrombosis Bariatric Surgery

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Assessement of cryopreserved red seabream (Pagrus major) sperm by flow cytometry and ultrastructure analysis

Assessement of cryopreserved red seabream (Pagrus major) spe...

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中国海洋湖沼动物学会鱼类学分会第七届会员代表大会暨朱元...

作者： Q.H.Liua,b j.lia S.C.Zhangc Z.Z.Xiaoa F.H.Dinga,b D.D.Yua,b X.Z.Xua,b (aCenter of Biotechnology R&D,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,P.R.China.bGraduate school of Chinese Academy of Sciences,Beijing 100031,P.R,China.cDepartment of Marine Biology,Ocean University of China,Qingdao 266003,P.R.China.)

Sperm cryopreservation in liquid nitrogen is a promising technique for germplasm conservation. The aim of this study was to evaluate the effect of two month cryopreservation on the quality of post-thaw sperm in red seabream(Pagrus major).The collected sperm were diluted 1:3 in Cortland extender containing 15% DMSO,placed in 2 mL cryovials,and frozen from 0oC to-150℃ at a cooling rate of 20℃/min by a Kryo-360-1.7 programmable freezer,then plunged into liquid nitrogen. After two month storage,the cryopreserved sperm were thawed in 40 oC water *** the present study,we used electron microscopy and flow cytometry to examine changes in ultrastructure, membrane integrity and mitochondrial function of the post-thaw sperm frozen with 15% *** the ultrastructural investigation,about 50% to 60% of spermatozoa showed normal morphology,10% to 20% were slightly damaged in some way,such as swelling or rupture of head,mid-piece and tail region as well as mitochondria,and the rest were damaged *** outcome was also obtained from flow cytomerical analysis,with rhodamine 123 to assess mitochondrial function and propidium iodide to assess plasma membrane *** 60% to 80% displayed both an intact membrane and an undamaged mitochondrion ***,the percentage of motile post-thaw sperm and fertilization capacity were also *** data from ultrastructural investigation and flow cytomertric analysis are in agreement with motility,fertilization rate and hatching *** this research, our results demonstrated that the method established for the cryopreservation of red seabream sperm should be of great value for the establishment of spermatozoa *** of ultrastructure investigation in combination with flow cytomerical analysis to evaluate the viability and mitochondrial function may be very valuable for the cryodamage assessment to fish sperm.

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Bee venom acupuncture reduces neuroinflammation modulating microglia/macrophage phenotype polarization in spinal cord injury compression model

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Neuroimmunology and Neuroinflammation 2019年第11期6卷 1-13页

作者： Raquel do Nascimento de Souza j.lia Miccolis Azevedo Lopes Lívia da Rocha Natalino Monteiro Raiana Andrade Quintanilha Barbosa Gabriela Hollmann Silvana Allodi Luis Carlos Reis MagdaAlves de Medeiros Department of Physiological Sciences Institute of Biology and Health SciencesFederal Rural University of Rio de JaneiroSeropedica 23897-000RJBrazil Carlos Chagas Filho Institute of Biophysics Federal University of Rio de JaneiroRio de Janeiro 21941-902RJBrazil.

Aim: The present study aimed to examine whether apipuncture (stimulation of acupuncture points with bee venom)at ST36 and GV3 acupoints promotes neuroprotection and reduces neuroinflammation by modulating M1 and M2 phenotype ***: Wistar rats were treated with bee venom (BV) (0.08 mg/kg) injection at acupoints ST36 and GV3 [BV (ST36 + GV3)-spinal cord injury (SCI)] or BV injection at non-acupoints [BV (NP)-SCI] or no treatment (CTL-SCI)after SCI by compression. The spinal cord mRNA expression of iNOS, Arg-1 and TGF-β was measured by real time PCR and the levels of IBA-1;BCL-2;NeuN e CNPase was measured by western blotting. Locomotor performance was measured by Basso, Beattie, and Bresnahan (BBB) and grid-walking ***: Apipuncture treatment was able to (1) ameliorate locomotor performance;(2) reduce inflammatory markers (Cox-2 levels) and activation of microglia and macrophages;(3) reduce the polarization of the M1 phenotype marker (iNOS) and increase M2 (Arg-1 and TGF-β) phenotypic markers;(4) promote neuroprotection by reducing the death of neurons and oligodendrocytes;and (5) increase the expression of the anti-apoptotic factor ***: Apipuncture treatment induces locomotor recovery and neuroprotection after the compression model of spinal cord injury. Further, it reduces neuroinflammation by decreasing M1 polarization and increasing M2 phenotype.

关键词： Acupuncture bee venom spinal cord injury compression microglia macrophage neuroinflammation

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Assessment of LLLT systemic effects on thyroid hormones function after dental titanium implant installation:An experimental rabbit model

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Natural Science 2013年第8期5卷 933-940页

作者： Luciano Mayer Fernando Vacilotto Gomes Carlos Eduardo Baraldi joao Batista Blessmann Weber Marília Gerhardt de Oliveira Department of Oral and Maxillofacial Surgery School of DentistryPontifícia Universidade Católica do Rio Grande do SulPorto AlegreBrazil Oral and Maxillofacial Surgery and Traumatology—Department of Surgery Universidade Federal do Rio Grande do SulSchool of DentistryRio Grande do SulBrazil Oral and Maxillofacial Surgery Service Hospital Cristo Redentor—Grupo Hospitalar ConceicaoPorto AlegreBrazil

This study aimed to assess the systemic effect of LLLT on thyroid gland functioning and consequently on calcium regulation through Triiodothyronine (T3) and Thyroxine (T4) measurements in rabbits’ serum. A total of thirty two New Zealand male rabbits were randomly distributed in four groups with eight animals each: control group C (nonirradiated animals), group EI (5 j/cm2 per session), group EII (10 j/cm2 per session) and group EIII (20 j/cm2 per session). All animals underwent lower left incisor extraction followed by immediate insertion of an osseintegrated implant, providing an equality of initial clinical condition between the groups. The experimental groups were irradiated with aluminium gallium arsenide diode laser (GaAlAs, λ = 830 nm, 50 mW, CW), during 13 days at each 48 hours, totalizing 7 sessions. Laboratorial T3 and T4 measurements were done in four distinct moments (before surgical procedure, immediately after surgical procedure, after the first LLLT session and after the last LLLT session) in all animals. The results obtained showed statistically significant differences in Triiodothyronine values between the groups throughout the experiment. It was concluded that the LLLT, in the protocol of irradiation used in this study, promoted a significantly alteration on rabbits’ serum hormonal levels.

关键词： Thyroid Gland Laser Therapy Low Level Thyroid Hormones Dental Implant

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Non-pharmacological management of pediatric functional abdominal pain disorders:Current evidence and future perspectives

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World journal of Clinical Pediatrics 2022年第2期11卷 105-119页

作者： Maria Luísa Cordeiro Santos Ronaldo Teixeira da Silva júnior Breno Bittencourt de Brito Filipe Antônio França da Silva Hanna Santos Marques Vinícius Lima de SouzaGonçalves Talita Costa dos Santos Carolina Ladeia Cirne Natália Oliveira e Silva Márcio Vasconcelos Oliveira Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45083-900BahiaBrazil

Functional abdominal pain disorders(FAPDs) are an important and prevalent cause of functional gastrointestinal disorders among children, encompassing the diagnoses of functional dyspepsia, irritable bowel syndrome, abdominal migraine, and the one not previously present in Rome Ⅲ, functional abdominal pain not otherwise specified. In the absence of sufficiently effective and safe pharmacological treatments for this public problem, non-pharmacological therapies emerge as a viable means of treating these patients, avoiding not only possible side effects, but also unnecessary prescription, since many of the pharmacological treatments prescribed do not have good efficacy when compared to placebo. Thus, the present study provides a review of current and relevant evidence on non-pharmacological management of FAPDs, covering the most commonly indicated treatments, from cognitive behavioral therapy to meditation, acupuncture, yoga, massage, spinal manipulation, moxibustion, and physical activities. In addition, this article also analyzes the quality of publications in the area, assessing whether it is possible to state if non-pharmacological therapies are viable, safe, and sufficiently well-based for an appropriate and effective prescription of these treatments. Finally, it is possible to observe an increase not only in the number of publications on the non-pharmacological treatments for FAPDs in recent years, but also an increase in the quality of these publications. Finally, the sample selection of satisfactory age groups in these studies enables the formulation of specific guidelines for this age group, thus avoiding the need for adaptation of prescriptions initially made for adults, but for children use.

关键词： Functional abdominal pain disorder Pediatrics Rome IV Behavioral intervention Nonpharmacological treatment Complementary medicine

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Reappraisal of Hepatocellular Adenoma from Federal Health System, Rio de janeiro, Brazil

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Open journal of Organ Transplant Surgery 2019年第1期9卷 1-10页

作者： Reinaldo Fernandes Klaus Steinbruck Danielle B. Delai jú lia R. N. Souza Igor Duque Daniel Barbosa Marcelo Enne Renato Cano Marcelo D’Oliveira Luiza Maciel Giuliano Bento Hepatobiliary Surgery Bonsucesso Federal Hospital Health Ministry Rio de Janeiro Brazil Surgery Department Antonio Pedro University Hospital Fluminense Federal University Niter&oacute i Brazil Hepatobiliary Surgery Bonsucesso Federal Hospital Health Ministry Rio de Janeiro Brazil Antonio Pedro University Hospital Fluminense Federal University Niter&oacute i Brazil Hepatobiliary Surgery Servidores do Estado Federal Hospital Health Ministry Rio de Janeiro Brazil Hepatobiliary Surgery Ipanema Federal Hospital Health Ministry Rio de Janeiro Brazil Nacional Cancer Institute Rio de Janeiro Brazil

Background: Hepatocellular adenoma is a rare liver tumor that may require surgical treatment in cases of hemorrhage or suspicion of malignant lesions. Aim: To analyze data from patients who underwent hepatectomy for hepatocellular adenoma (HCA) in Rio de janeiro, Brazil. Methods: From january 2005 to March 2019, sixty-nine patients with HCA underwent hepatectomy at centers in Rio de janeiro. They were included in the analysis patients undergoing hepatectomy with pathological diagnosis of hepatocellular adenoma and excluded patients with hepatectomy with anatomopathological diagnosis other than hepatocellular adenoma, mainly nodular focal hyperplasia. Data related to patients, tumor and surgery were analyzed retrospectively. Results: Sixty patients (87%) were female and nine were male. Among women, 83% had a history of contraceptive use;among men, only one had an androgen intake history. Overall mean age was 36.4 years (15 - 49), with men older than women (33.9 ± 8.14 years vs. 40.4 ± 6.27 years;P = 0.02). Forty one patients reported abdominal pain, associated or not to other symptoms;32% had an episode of hemorrhage;28 were asymptomatic with an incidental radiological finding. In total, 45 patients presented only one lesion and overall mean size was 8.1 cm (2 - 31);tumors were larger among men (mean size 12.9 ± 9.86 cm vs. 7.7 ± 4.58 cm;P = 0.009). Twenty one surgeries were laparoscopic. Hepatocellular carcinoma (HCC) was identified in three specimens and the incidence was higher among men (22.2% vs. 1.6%;P = 0.042) and in tumors larger than 20 cm (66.6% vs. 0.02%). There was no perioperative mortality. Two of the three patients with HCC died with extrahepatic recurrence;the remaining patient is well after 36 months. Overall mean follow-up time was 14.2 months (2 - 76). Conclusion: Male patients with HCA were older and had larger tumors when compared to females. Incidence of HCC was higher among men and in lesions larger than 20 cm. Male patients with HCA should be

关键词： Hepatocellular Adenoma Hepatocellular Carcinoma Hepatectomy

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Flowering of Sugarcane Genotypes under Different Artificial Photoperiod Conditions

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American journal of Plant Sciences 2015年第3期6卷 456-463页

作者： Maria Letícia G. Melloni Maria Natália G. Melloni Maximiliano S. Scarpari júlio César Garcia Marcos G. A. Landell Luciana R. Pinto Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Jaboticabal Brazil Sugarcane Research Center Instituto Agron&ocircmico de Campinas (IAC) Ribeir&atildeo Preto Brazil

Flowering is essential in any breeding programme, particularly in genetic introgression programmes in which flowering synchronism is required. In this work, 16 sugarcane genotypes were evaluated using three different flowering-inductive photoperiod treatments (30 s, 45 s, and 1 min of a daily photoperiod decrease from 12 h 55 of light). Each genotype was planted in 43 L pots (equal proportion of soil, sand, and substrate) with three tillers per pot. Plants with 4 to 6 internodes were placed in a photoperiod facility with three controlled chambers, each chamber containing all 16 genotypes (one treatment per chamber). The temperature range (21°C to 32°C) and humidity were the same for all of the treatments. The flower induction started in September 2010 and ended in April 2011. The plant elongation and flag leaf and inflorescence emergence were recorded and the pollen viability was evaluated by using the iodine staining method. The photoperiod facility provided suitable conditions for flowering, as the plants in the three treatments successfully flowered. The genotypes in the three treatments behaved differently in relation to the date of panicle emergence, with the treatment of 45 s showing better results. The results here presented contribute to synchronise flowering for desired sugarcane crosses, particularly those from introgression programmes between commercial cultivars and species from the Saccharum complex.

关键词： Artificial Induction Leaf Flag Photoperiod Facilities Physiologic Flowering Pollen

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Quality of Semi-Prepared Products from Rainbow Trout Waste(Onchorynchus mykiss)by Using Different Technological Strategics

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Food and Nutrition Sciences 2014年第6期5卷 571-580页

作者： Karoline Ribeiro Palmeira Eliane Teixeira Mársico Laís Doro Mosar Lemos Claudia Emília Teixeira Vania Margaret Flosi Paschoalin Maria Lúcia Guerra Monteiro Carlos Adam Conte júnior Department of Food Technology Fluminense Federal UniversityRio de JaneiroBrazil Department of Biochemistry Chemistry InstituteFederal University of Riode JaneiroRio de JaneiroBrazil

The consumption of freshwater fish and fish products has gradually grown worldwide over the last decades, generating a proportional waste increase. The objective of the present study was to assess the chemical and bacteriological quality of restructured fish product, meatball-type, prepared with rainbow trout (Onchorynchus mykiss) waste added of 1% transglutaminase (MTG), 4% textured soy protein (TSP) and replacing part of the sodium chloride with potassium chloride (75%/25%) as described below: T1—starch addition (control);T2—MTG addition (1%);T3—soy protein addition (4%);T4—soy protein addition (4%) and MTG addition (1%);T5—soy protein addition (4%), MTG addition (1%) and partial replacement of salt (75% NaCl/25% KCl). Total aerobic mesophilic bacteria (TAMB), 2-thiobarbituric acid reactive substances (TBARS), pH determination and quantification of biogenic amines were performed on the day after manufacturing (P0) and after 60 days of storage (P1) at -25℃?± 2℃. The results showed that there was no significant difference (p < 0.05) of microbiological quality, TBARS and pH after storage. T4 presented the lowest total biogenic amine content (256.84 mg/kg) whereas T3 and T5 had the highest value (791.36 and 707.19 mg/kg, respectively) in this parameter. Putrescine was the biogenic amine that presented the highest concentration (504.00 mg/kg) in T3 and cadaverine that presented the smallest concentration (0.36 mg/kg) in T4. The use of technological strategies for developing new products with non-commercial fillets kept the most standards, having changes only in some biogenic amines.

关键词： Oncorhynchus mykiss Soy Protein Microbial Transglutaminase Salt Replacer Biogenic Amine

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Risk factors associated with inflammatory bowel disease: A multicenter case-control study in Brazil

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World journal of Gastroenterology 2020年第25期26卷 3611-3624页

作者： Valéria Cristina Loureiro Salgado Ronir Raggio Luiz Neio Lucio Fernandes Boéchat Isabella Sued Leao Bianca do Carmo Schorr joséMiguel Luz Parente Daniela Calado Lima Eduardo Santos Silveira júnior Genoile Oliveira Santana Silva Neogélia Pereira Almeida Andrea Vieira Maria Luiza Queiroz de Bueno júlio Maria Chebli érika Ruback Bertges Luísa Martins da Costa Brugnara Columbano junqueira Neto Stefania Burjack Gabriel Campbell Luana Letiza Discacciati joao paulo Silva Cézar Tiago Nunes Gilaad G Kaplan Cyrla Zaltman Division of Gastroenterology Department of Internal MedicineClementino Fraga Filho HospitalFederal University of Rio de JaneiroRio de Janeiro 21940-230Brazil Institute for Studies in Public Health Federal University of Rio de JaneiroRio de Janeiro 21940-230Brazil Multidisciplinary Research Laboratory Clementino Fraga Filho HospitalInstitute of Thoracic DiseasesFaculty of MedicineFederal University of Rio de JaneiroRio de Janeiro 21940-230Brazil Division of Gastroenterology Department of Internal MedicineHospital UniversityFaculty of MedicineFederal University of PiauíPiauí64049-550Brazil Almeida Division of GastroenterologyInflammatory Bowel Disease Outpatient ClinicRoberto Santos General Hospital(HGRS)of the Bahia State Department of HealthBahia 40110-060Brazil Division of Gastroenterology Inflammatory Bowel Disease Outpatient ClinicIrmandade Santa Casa da Misericórdia of São PauloSão Paulo 01221020Brazil Division of Gastroenterology Department of Internal MedicineHospital UniversityFaculty of MedicineFederal University of Juiz de ForaMinas Gerais 36036-247Brazil Division of Gastroenterology Inflammatory Bowel Disease Outpatient ClinicFederal District Base HospitalBrasília 70330-150Brazil Gastrointestinal Physiology Institute of Nutritional ScienceNestle Research CenterLausanne 1000Switzerland Departments of Medicine and Community Health Sciences University of CalgaryCalgary T2N4Z6Canada

BACKGROUND The etiology of inflammatory bowel disease(IBD)is unknown,but it is believed to be *** hygiene hypothesis proposes that better hygiene conditions would lead to less infectious disease during childhood and favor the development of immune-mediated *** To test the hygiene hypothesis in IBD by assessing the environmental risk factors associated with IBD development in different regions of Brazil with diverse socioeconomic development *** A multicenter case-control study was carried out with 548 Crohn’s disease(CD)and 492 ulcerative colitis(UC)outpatients and 416 healthy controls,from six IBD centers within different Brazilian states at diverse socioeconomic development stages.A semi-structured questionnaire with 87 socioeconomic and environmental questions was *** regression model was created to assess the odds ratio(OR)with P value and 95%confidence intervals(CI).RESULTS Predictive variables for both diseases(CD and UC)were women[odd ratios(OR)=1.31;OR=1.69],low monthly family income(OR=1.78;OR=1.57),lower number of cohabitants(OR=1.70;OR=1.60),absence of vaccination(OR=3.11;OR=2.51),previous history of bowel infections(OR=1.78;OR=1.49),and family history of IBD(OR=5.26;OR=3.33).Associated risk factors for CD were age(18-39 years)(OR=1.73),higher educational level(OR=2.22),absence of infectious childhood diseases(OR=1.99).The UC predictive variables were living in an urban area(OR=1.62),inadequate living conditions(OR=1.48)and former smokers(OR=3.36).Appendectomy was a risk factor for CD(OR=1.58)with inverse association with UC(OR=4.79).Consumption of treated and untreated water was associated with risk of CD(OR=1.38)and UC(OR=1.53),*** This is the first examining environmental exposures as risk factors for inflammatory bowel disease in *** of the variables associated with disease risk support the role of the hygiene hypothesis in IBD development.

关键词： Crohn’s disease Ulcerative colitis Risk factors Environmental factors Hygiene hypothesis

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Dietary Omega-3 Fatty Acids Deficiency Affects the Glutamatergic Transport System in Rat Retina:Modulatory Effects after High Intraocular Pressure

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Food and Nutrition Sciences 2013年第9期4卷 195-201页

作者： Letícia V.Siqueira Eduardo P.Rico Mário C.Bulla Luciano Bellini Luiz C.L.Silveira Lúcia Vinadé Diogo O.Souza j.lia D.Moreira Biochemistry Department Basic Sciences of Health InstituteFederal University of Rio Grande do SulPorto AlegreBrazil Phy-siology Department Biological Science CenterFederal University of ParáBelémBrazil Master Graduation Program in Biological Sciences Campus Sao GabrielFederal University of Pampa—UNIPAMPASao GabrielBrazil Department of Nutrition Health Sciences CentreFederal University of Santa CatarinaCampus Universitário TrindadeFlorianópolisBrazil

Glutamate excitotoxicity has been postulated as a putative mechanism involved in the physiopathology of glaucoma, a disease that can cause retinal cell damage. Thus, the modulation of glutamatergic parameters is a putative therapeutic target to prevent excitotoxic retinal injury. Here, we investigated the effect of dietary omega-3 fatty acids (w3) in the retinal glutamate transport system in basal and ischemic conditions. Female Wistar rats were divided into two groups: w3 diet (w3 group) and w3 deficient-diet (D group). Their pups, at 60 days old, were used for the experiments. Retinal ischemia, a mechanism involved in the physiopathology of glaucoma, was induced by high intraocular pressure (HIOP, 140 180 mmHg for 45 min) to impair retinal blood flow. Analyses were performed 7 days after ischemia. The D group showed a decreased glutamate uptake in basal conditions and after HIOP when compared to the w3 group. After HIOP, there was a decrease in glutamate uptake in the D group that was not observed in the w3 group (p < 0.005). Concerning glutamate transporters, the w3 group presented higher levels of GLT-1 compared to the D group in basal and ischemic conditions. After HIOP, EAAC1 was increased in both groups, while GLT-1 increased only in the D group, compared to basal levels. GLAST and EAAT5 presented no alterations. The modulation of the glutamatergic system by dietary w3 fatty acids points to a potential mechanism by which w3 PUFAs exert beneficial effects in the retina.

关键词： N-3 PUFAs Retina Ischemia HIOP Glaucoma Glutamate Transporters

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