Bee venom acupuncture reduces neuroinflammation modulating microglia/macrophage phenotype polarization in spinal cord injury compression model

作     者：Raquel do Nascimento de Souza Júlia Miccolis Azevedo Lopes Lívia da Rocha Natalino Monteiro Raiana Andrade Quintanilha Barbosa Gabriela Hollmann Silvana Allodi Luis Carlos Reis MagdaAlves de Medeiros Raquel do Nascimento de Souza;Júlia Miccolis Azevedo Lopes;Lívia da Rocha Natalino Monteiro;Raiana Andrade Quintanilha Barbosa;Gabriela Hollmann;Silvana Allodi;Luis Carlos Reis;Magda Alves de Medeiros

作者机构：Department of Physiological SciencesInstitute of Biology and Health SciencesFederal Rural University of Rio de JaneiroSeropedica 23897-000RJBrazil Carlos Chagas Filho Institute of BiophysicsFederal University of Rio de JaneiroRio de Janeiro 21941-902RJBrazil. 

出 版 物：《Neuroimmunology and Neuroinflammation》 (神经免疫与神经炎症（英文版）)

年 卷 期：2019年第6卷第11期

页      面：1-13页

学科分类：1006[医学-中西医结合] 1005[医学-中医学] 100502[医学-中医临床基础] 10[医学] 100602[医学-中西医结合临床] 

基　　金：This work was supported by FAPERJ(Research support foundation in the state of Rio de Janeiro)(grand number.111.616/2010) 

主　　题：Acupuncture bee venom spinal cord injury compression microglia macrophage neuroinflammation 

摘      要：Aim: The present study aimed to examine whether apipuncture (stimulation of acupuncture points with bee venom)at ST36 and GV3 acupoints promotes neuroprotection and reduces neuroinflammation by modulating M1 and M2 phenotype ***: Wistar rats were treated with bee venom (BV) (0.08 mg/kg) injection at acupoints ST36 and GV3 [BV (ST36 + GV3)-spinal cord injury (SCI)] or BV injection at non-acupoints [BV (NP)-SCI] or no treatment (CTL-SCI)after SCI by compression. The spinal cord mRNA expression of iNOS, Arg-1 and TGF-β was measured by real time PCR and the levels of IBA-1;BCL-2;NeuN e CNPase was measured by western blotting. Locomotor performance was measured by Basso, Beattie, and Bresnahan (BBB) and grid-walking ***: Apipuncture treatment was able to (1) ameliorate locomotor performance;(2) reduce inflammatory markers (Cox-2 levels) and activation of microglia and macrophages;(3) reduce the polarization of the M1 phenotype marker (iNOS) and increase M2 (Arg-1 and TGF-β) phenotypic markers;(4) promote neuroprotection by reducing the death of neurons and oligodendrocytes;and (5) increase the expression of the anti-apoptotic factor ***: Apipuncture treatment induces locomotor recovery and neuroprotection after the compression model of spinal cord injury. Further, it reduces neuroinflammation by decreasing M1 polarization and increasing M2 phenotype.