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Triose phosphate export from chloroplasts and cellular sugar content regulate anthocyanin biosynthesis during high light acclimation

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Plant Communications 2023年第1期4卷 42-61页

作者： Max-Emanuel Zirngibl Galileo Estopare Araguirang Anastasia Kitashova Kathrin j.hnke Tobias Rolka Christine Kühn Thomas Nägele Andreas S.Richter University of Rostock Institute for BiosciencesPhysiology of Plant MetabolismAlbert-Einstein-Strasse 318059 RostockGermany Humboldt-Universit€at zu Berlin Institute of BiologyPhysiology of Plant Cell OrganellesPhilippstrasse 1310115 BerlinGermany Ludwig-Maximilians-Universit€at M€unchen Faculty of BiologyPlant Evolutionary Cell Biology82152 Planegg-MartinsriedGermany

Plants have evolvedmultiple strategies to cope with rapid changes in the *** high light(HL)acclimation,the biosynthesis of photoprotective flavonoids,such as anthocyanins,is ***,the exact nature of the signal and downstreamfactors forHL induction of flavonoid biosynthesis(FB)is still under ***,we show that carbon fixation in chloroplasts,subsequent export of photosynthates by triose phosphate/phosphate translocator(TPT),and rapid increase in cellular sugar content permit the transcriptional and metabolic activation of anthocyanin biosynthesis during HL *** combination with genetic and physiological analysis,targeted and whole-transcriptome gene expression studies suggest that reactive oxygen species and phytohormones play only a minor role in rapid HL induction of the anthocyanin branch of *** addition to transcripts of FB,sugar-responsive genes showed delayed repression or induction in tpt-2 during HL treatment,and a significant overlapwith transcripts regulated by SNF1-related protein kinase 1(SnRK1)was observed,including a central transcription factor of *** of mutants with increased and repressed SnRK1 activity suggests that sugar-induced inactivation of SnRK1 is required for HL-mediated activation of anthocyanin *** study emphasizes the central role of chloroplasts as sensors for environmental changes as well as the vital function of sugar signaling in plant acclimation.

关键词： flavonoid biosynthesis acclimation sugar signaling high light anthocyanin SnRK1

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接触斑点对蜗轮副承载能力的影响

机械传动 2014年第10期38卷 9-13,17页

作者： Hermes j..Hhn B.-R. Stahl K. Monz A. 陈金山 SEW-Eurodrive传动设备德国有限公司德国布鲁克塞尔76646 慕尼黑工业大学齿轮研究中心(FZG) 德国加兴85748 德国IMO集团公司德国格蕾姆斯多夫91350 浙江台玖精密机械有限公司浙江嘉兴314500

根据现有的技术发展水平,只有当蜗轮副的齿面完全啮合,才能评估其抗点蚀能力。但实际上,在蜗轮与蜗杆初始啮合时,它们只有一个较小的啮合斑点。由于时间和经费的关系,通过长时间轻载跑合使其达到完全啮合一般不太可能。在初期,由于接触... 详细信息

根据现有的技术发展水平,只有当蜗轮副的齿面完全啮合,才能评估其抗点蚀能力。但实际上,在蜗轮与蜗杆初始啮合时,它们只有一个较小的啮合斑点。由于时间和经费的关系,通过长时间轻载跑合使其达到完全啮合一般不太可能。在初期,由于接触面较小,齿部的接触应力会很大,有可能会在啮合处的表面产生一条微观裂纹,随后裂纹继续发展使材料从蜗轮上剥落,从而形成点蚀。较小的啮合斑点与点蚀的关系迄今还没有被系统的分析与研究,因此蜗轮轮齿也不能很好的进行优化。通过理论和实践相结合,提一种新的、可适用于不同尺寸、不同接触斑点位置以及齿形的计算方法,以弥补这一空白领域。

关键词：蜗轮蜗杆点蚀

Role of the intracellular receptor domain of gp130 (exon 17) in human inflammatory bowel disease

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World j..rnal of Gastroenterology 2005年第8期11卷 1196-1199页

作者： Christoph j..uernhammer Kathrin Zitzmann Fabian Schnitzler j..ia Seiderer Peter Lohse George Vlotides Dieter Engelhardt Michael Sackmann Burkhard G(o|¨)ke Thomas Ochsenkühn Department of Internal Medicine Ⅱ-Grosshadern Department of Clinical Chemistry-Grosshadern

AIM: To study the role of the intracellular receptor domain of gp130 in human inflammatory bowel disease (IBD).METHODS: We amplified and sequenced the complete exon 17 of the human gp130 gene in 146 patients with IBD. According to clinical and histopathological signs,the 146 patients with IBD were classified as having Crohn's disease (n = 73) or ulcerative colitis (n = 63),or as indeterminate status (n = 10).RESULTS: No mutations in exon 17 of the gp130 gene could be detected in any of the 146 patients with IBD ***: There is no evidence that mutations in exon 17 of the gp130 gene are involved in the pathogenesis of human IBD.

关键词： Exon 17 Inflammatory bowel disease gp130 gene

High-resolution two-photon polymerization:the most versatile technique for the fabrication of microneedle arrays

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Microsystems & Nanoengineering 2021年第5期7卷 1-17页

作者： Zahra Faraj..Rad Philip D.Prewett Graham j..avies School of Mechanical and Elearical Engineering University of Southern QueenslandSpringfield CentralQLD 4300Australia Department of Mechanical Engineering University of BirminghamBirmingham B152TTUK Oxacus Ltd Dorchester-on-Thames 0X107HNUK Faculty of Engineering UNSW AustraliaKensingtonNSW 2052Australia College of Engineering and Physical Sciences School of EngineeringUniversity of BirminghamBirmingham B152TTUK

Microneedle patches have received much interest in the last two decades as drug/vaccine delivery or fluid sampling systems for diagnostic and monitoring *** are manufactured using a variety of additive and subtractive micromanufacturing *** the last decade,much attention has been paid to using additive manufacturing techniques in both research and industry,such as 3D printing,fused deposition modeling,inkj.. printing,and two-photon polymerization(2PP),with 2PP being the most flexible method for the fabrication of microneedle arrays.2PP is one of the most versatile and precise additive manufacturing processes,which enables the fabrication of arbitrary three-dimensional(3D)prototypes directly from computer-aided-design(CAD)models with a resolution down to 100 *** to its unprecedented flexibility and high spatial resolution,the use of this technology has been widespread for the fabrication of bio-microdevices and bio-nanodevices such as microneedles and microfluidic *** is a pioneering transformative technology that facilitates the fabrication of complex miniaturized structures that cannot be fabricated with established multistep manufacturing methods such as inj..tion molding,photolithography,and ***,microstructures are designed according to structural and fluid dynamics considerations rather than the manufacturing constraints imposed by methods such as machining or etching *** article presents the fundamentals of 2PP and the recent development of microneedle array fabrication through 2PP as a precise and unique method for the manufacture of microstructures,which may overcome the shortcomings of conventional manufacturing processes.

关键词： resolution needle additive

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快乐之旅 XROYG复活节岛远征纪实

现代通信 2013年第3期 81-85页

作者： j.hn·Warburton 阮东升(编译)

BA6QH：此次复活节岛XROYG远征结束后，远征队成员之一的G41RN约翰袄伯顿先生回国给CDXC会刊专门撰文介绍了这次DX远征。对于这次复活节岛XROYG远征的具体情况，笔者相信无论是否与其通联成功，国内DXer都会很感兴趣。当约翰先生得知笔... 详细信息

BA6QH：此次复活节岛XROYG远征结束后，远征队成员之一的G41RN约翰袄伯顿先生回国给CDXC会刊专门撰文介绍了这次DX远征。对于这次复活节岛XROYG远征的具体情况，笔者相信无论是否与其通联成功，国内DXer都会很感兴趣。当约翰先生得知笔者准备把他们的这次远征介绍给中国的HAM后非常高兴，不仅同意笔者翻译转载他的文章，还提供了大量的帮助。笔者在朋友的帮助下尝试着将这篇远征纪实为国内HAM翻译过来，希望能对感兴趣的朋友有所帮助。特别感谢远征队成员之一，G3TXF奈吉尔先生，他向我提供了一些原始图片作为素材，使我们能更加全面地了解这次远征活动。

关键词：复活节岛 HAM 国内翻译

On the combined effect of soil fertility and topography on tree growth in subtropical forest ecosystems—a study from SE China

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j..rnal of Plant Ecology 2017年第1期10卷 111-127页

作者： Thomas Scholten Philipp Goebes Peter Kühn Steffen Seitz Thorsten Assmann j..gen Bauhus Helge Bruelheide Francois Buscot Alexandra Erfmeier Markus Fischer Werner Härdtle j..-Sheng He Keping Ma Pascal A.Niklaus Michael Scherer-Lorenzen Bernhard Schmid Xuezheng Shi Zhengshan Song Goddert von Oheimb Christian Wirth Tesfaye Wubet Karsten Schmidt Department of Geosciences Soil Science and GeomorphologyUniversity of TübingenRümelinstraße 19-2372070 TübingenGermany Institute of Ecology Leuphana University LüneburgScharnhorststr.121335 LüneburgGermany Faculty of Forest and Environmental Sciences SilvicultureUniversity of FreiburgTennenbacherstraße 479085 Freiburg im BreisgauGermany Institute of Biology/Geobotany and Botanical Garden Martin Luther University Halle WittenbergAm Kirchtor 106108 HalleGermany German Centre for Integrative Biodiversity Research(iDiv)Halle-j..a-Leipzig Deutscher Platz 5e04103 LeipzigGermany Helmholtz Centre for Environmental Research UFZ Theodor-Lieser-Straße 406120 HalleGermany Department of Plant Sciences University of BernAltenbergrain 213013 BernSwitzerland Peking University No.5 Yiheyuan RoadHaidian DistrictBeijing 100871China Institute of Botany Chinese Academy of SciencesNo.20 NanxincunXiangshanBeijing 100093China Institute of Evolutionary Biology and Environmental Studies University of ZürichWinterthurerstrasse 1908057 ZürichSwitzerland Faculty of Biology Department of GeobotanyUniversity of FreiburgSchänzlestraße 179104 FreiburgGermany Institute of Soil Science Chinese Academy of SciencesNo.71 East Beijing RoadNanjingChina TU Dresden Biodiversity and Nature ConservationPienner Straße 701737 TharandtGermany Department of Systematic Botany and Functional Biodiversity University of LeipzigJohannisallee 2104103 LeipzigGermany

Aims The aim of our research was to understand small-scale effects of topography and soil fertility on tree growth in a forest biodiversity and ecosystem functioning(BEF)experiment in subtropical SE *** Geomorphometric terrain analyses were carried out at a spatial resolution of 5×5 *** samples of different depth increments and data on tree height were collected from a total of 566 plots(667 m2 each).The soils were analyzed for carbon(soil organic carbon[SOC]),nitrogen,acidity,cation exchange capacity(CEC),exchangeable cations and base saturation as soil fertility *** plots were classified into geomorphological *** of variance and linear regressions were applied to all terrain,soil fertility and tree growth *** Findings In general,young and shallow soils and relatively small differences in stable soil properties suggest that soil erosion has truncated the soils to a large extent over the whole area of the *** explains the concurrently increasing CEC and SOC stocks downslope,in hollows and in ***,colluvial,carbon-rich sediments are missing widely due to the convexity of the footslopes caused by uplift and removal of eroded sediments by adj..ent *** results showed that soil fertility is mainly influenced by ***-Carlo flow accumulation(MCCA),curvature,slope and aspect significantly affected soil ***,soil fertility was affected by the different geomorphological positions on the experimental sites with ridge and spur positions showing lower exchangeable base cation contents,especially potassium(K),due to *** geomorphological effect of soil fertility is most pronounced in the topsoil and decreases when considering the subsoil down to 50 cm *** soil fertility attributes affect tree height after 1-2 years of growth,among which C stocks proved to be most important while pH_(KCl)and CEC only played minor ***,soil acidity and a hig

关键词： soil fertility topography soil erosion matter transport biodiversity DSM carbon stocks tree forest BEF-China China

Attractivity in a Delayed Three-species Ratio-dependent Predator-prey System without Dominating Instantaneous Negative Feedback

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Acta Mathematicae Applicatae Sinica 2003年第2期19卷 317-332页

作者： RuiXu Lan-sunChen M.A.j..haplain DepartmentofMathematics MechanicalEngineeringCollegeShijiazhuang050003China InstituteofMathematics AcademyofMathematicsandSystemSciencesChineseAcademyofSciencesBeijing100080China DepartmentofMathematics UniversityofDundeeDundeeDD14HNUK

Abstract A delayed three-species ratio-dependent predator-prey food-chain model without dominating instantaneous negative feedback is investigated. It is shown that the system is permanent under some appropriate condi... 详细信息

关键词： Keywords Time delay uniform persistence global attractivity

Customized molecular tools to strengthen metabolic engineering of cyanobacteria

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Green Carbon 2024年第2期2卷 149-163页

作者： Stephan Klähn Franz Opel Wolfgang R.Hess Department of Solar Materials Biotechnology Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany Genetics and Experimental Bioinformatics Albert-Ludwig University FreiburgFreiburg im Breisgau 79104Germany

Cyanobacteria are promising oxygenic phototrophs for the production of various *** their(photo)biotechnological exploitation,molecular tools are required,such as,for the introduction and expression of heterologous genes,or the modulation of enzyme activities or entire *** and strategies for the development of photosynthetic biomanufacturing technologies based on cyanobacteria have been extensively reviewed,as well as certain specialized aspects of their genetic ***,options for metabolic engineering of specific cyanobacterial cells are still less developed than those for other bacteria of biotechnological *** addition to the standard genetic toolbox for“classical”metabolic engineering,we emphasize certain aspects,including recently developed vector systems for the extrachromosomal maintenance of genes and approaches based on clustered regularly interspaced short palindromic repeats(CRISPR)*** highlight the development of custom molecular tools for specific strains or products,discuss the emerging use of small regulatory proteins that appear promising for advanced metabolic engineering approaches to promote specific product formation,and provide an overview of suitable online ***,we discuss the current trends in this field and indicate their potential,such as using suitable product sensors that enable systematic screening,and optimization approaches.

关键词： Metabolic engineering Cyanobacteria Synthetic plasmids Regulatory proteins Sensors

Achieving exceptional wear resistance in a crack-free high-carbon tool steel fabricated by laser powder bed fusion without pre-heating

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j..rnal of Materials Science & Technology 2023年第25期156卷 1-19页

作者： Konrad Kosiba Daniel Wolf Matthias Bönisch Kai Neufeld Ruben Hühne Tobias Gustmann j..ef Bednarčík Hongyu Chen Xiaoliang Han Volker Hoffmann Lukas Beyer Uta Kühn Sergio Scudino Lars Giebeler j..ia K.Hufenbach Leibniz Institute for Solid State and Materials Research (IFW) Dresden E.V. Helmholtzstr. 20Dresden 01069Germany Department of Materials Engineering KU LeuvenKasteelpark Arenberg 44bus 2450Leuven 3001Belgium Department of Condensed Matter Physics Institute of PhysicsP.J. Safarik University in KosicePark Angelinum 9Kosice 04154Slovakia Institute of Experimental Physics Slovak Academy of ScienceWatsonova 47Kosice 040 01Slovakia Key Laboratory of Impact and Safety Engineering of Ministry of Education of China Ningbo UniversityNingbo 315211China TU Bergakademie Freiberg Institute of Materials ScienceGustav-Zeuner-Str. 5Freiberg 09599Germany

Laser powder bed fusion(LPBF)for the fabrication of dense components used for tooling applications,is highly *** stresses,which evolve in the additively manufactured part,are inherent to LPBF *** additional stress contribution in high-carbon steels arises from the austenite-to-martensite phase transformation,which may eventually lead to cracking or even *** an alternative to pre-heating the base plate,which is not striven by industry,lowering the martensite content which forms in the part,is essential for the fabrication of dense parts by LPBF of high-carbon tool steels which are then adapted to *** this study,a successful strategy demonstrates the processing of the Fe85Cr4Mo1V1W8C1(wt%)high-carbon steel by LPBF into dense parts(99.8%).The hierarchical microstructure consists of austenitic and martensitic grains separated by elemental segregations in which nanoscopic carbide particles form a network.A high density of microsegregation was observed at the molten pool boundary ultimately forming a *** LPBF-fabricated steel shows a yield strength,ultimate compressive stress,and total strain of 1210 MPa,3556 MPa,and 27.4%,*** mechanical and wear performance is rated against the industrially employed and highly wear-resistant 1.2379 tool steel taken as the *** its lower macro-hardness,the LPBF steel(58.6 HRC,0.0061 mm^(3) Nm^(-1))shows a higher wear resistance than the reference steel(62.6 HRC,0.0078 mm^(3) Nm^(-1)).This behavior results from the wear-induced formation of martensite in a microscale thick layer directly at the worn surface,as it was proven via high-energy X-ray diffraction mapping.

关键词： Additive manufacturing Laser powder bed fusion Steel Wear TRIP