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A complete reference genome for the soybean ***

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Plant Communications 2024年第2期5卷 19-22页

作者： Yicheng Huang Dal-Hoe Koo Yizhou Mao Eliot M.herman jianwei Zhang Monica A.Schmidt National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Wheat Genetics Resource Center Department of Plant PathologyKansas State UniversityManhattanKS 66506USA BIO5 Institute School of Plant SciencesUniversity of ArizonaTucsonAZ 85721USA

Dear Editors,Soybeans are a global commodity for their edible protein and vegetable *** global population is predicted to be 9.7 billion by 2050(UN,2022),with a concomitant drastic increase in protein *** already 2.4 billion people suffering from food insecurity(FAO et al.,2023),there is an urgent need to meet future production demands for plant-based proteins.

关键词： commodity soybean urgent

Precise genome-editing in human diseases:mechanisms,strategies and applications

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Signal Transduction and Targeted Therapy 2024年第3期9卷 1065-1085页

作者： Yanjiang Zheng Yifei Li Kaiyu Zhou Tiange Li Nathan j.VanDusen Yimin Hua Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE Department of PediatricsWest China Second University HospitalSichuan UniversityChengduSichuan 610041China Department of Cardiovascular Surgery West China HospitalSichuan UniversityChengduSichuan 610041China Department of Pediatrics Herman B Wells Center for Pediatric ResearchIndiana University School of MedicineIndianapolisIN 46202USA

Precise genome-editing platforms are versatile tools for generating specific,site-directed DNA insertions,deletions,and *** continuous enhancement of these tools has led to a revolution in the life sciences,which promises to deliver novel therapies for genetic *** genome-editing can be traced back to the 1950s with the discovery of DNA’s doublehelix and,after 70 years of development,has evolved from crude in vitro applications to a wide range of sophisticated capabilities,including in vivo ***,precise genome-editing faces constraints such as modest efficiency,delivery challenges,and off-target *** this review,we explore precise genome-editing,with a focus on introduction of the landmark events in its history,various platforms,delivery systems,and ***,we discuss the landmark events in the history of precise ***,we describe the current state of precise genome-editing strategies and explain how these techniques offer unprecedented precision and versatility for modifying the human ***,we introduce the current delivery systems used to deploy precise genome-editing components through DNA,RNA,and ***,we summarize the current applications of precise genome-editing in labeling endogenous genes,screening genetic variants,molecular recording,generating disease models,and gene therapy,including ex vivo therapy and in vivo therapy,and discuss potential future advances.

关键词： precise generating editing

Understanding Genetic Load in Potato for Hybrid Diploid Breeding

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Molecular Plant 2019年第7期12卷 896-898页

作者： Christian W.B.Bachem herman j.van Eck Michiel E.de Vries Plant Breeding Wageningen University and Research Wageningen the Netherlands Solynta Wageningen the Netherlands

Recently, Zhang et al.(2019) have managed to provide abundant evidence to support long-held theories regarding mechanisms of inbreeding depression and potential effects of heterosis that are likely to have a considerable impact on potato breeding in the future (Figure 1). Despite its large genetic variation regarding yield, harvest index, and water use efficiency, potato breeding has attained only negligible advances in the most important breeding goals such as yield, tuber quality, and abiotic stress tolerance, and only modest progress in pathogen resistance. This low level of improvement is clearly due to the complexity of breeding a heterozygous tetraploid outbreeder rather than to the lack of genetic potential available in wild and cultivated material. In contrast, breeding at the diploid level, in particular in combination with self-compatibility, will allow the fixation of beneficial alleles to homozygosity.

关键词： Genetic Load Potato Hybrid Diploid Breeding

Management of borderline and locally advanced pancreatic cancer:Where do we stand?

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World journal of Gastroenterology 2014年第9期20卷 2255-2266页

作者： jin He Andrew j Page Matthew Weiss Christopher L Wolfgang joseph M herman Timothy M Pawlik Department of Surgery Johns Hopkins Hospital Baltimore MD 21287 United States Department of Radiation Oncology Johns Hopkins Hospital Baltimore MD 21287 United States

Many patients with pancreas cancer present with locally advanced pancreatic cancer(LAPC).The principle tools used for diagnosis and staging of LAPC include endoscopic ultrasound,axial imaging with computed tomography and magnetic resonance imaging,and diagnostic *** definition of resectability has historically been vague,as there is considerable debate and controversy as to the definition of *** the patient with LAPC,there is some level of involvement of the surrounding vascular structures,which include the superior mesenteric artery,celiac axis,hepatic artery,superior mesenteric vein,or portal *** feasible,most surgeons would recommend possible surgical resection for patients with borderline LAPC,with the goal of an R0 *** initially unresectable LAPC,neoadjuvant should be strongly ***,these patients should be offered neoadjuvant therapy,and the tumor should be assessed for possible response and eventual *** efficacy of neoadjuvant therapy with this approach as a bridge to potential curative resection is broad,ranging from 3%-79%.The different modalities of neoadjuvant therapy include sin-gle or multi-agent chemotherapy combined with radiation,chemotherapy alone,and chemotherapy followed by chemotherapy with *** review focuses on patients with LAPC and addresses recent advances and controversies in the field.

关键词： Pancreas Locally advanced Chemotherapy Radiation Irreversible electroporation Pancreatic cancer

Absolute configuration of podophyllotoxone and its inhibitory activity against human prostate cancer cells

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Chinese journal of Natural Medicines 2015年第1期13卷 59-64页

作者： LI juan FENG juan LUO Cheng herman Ho-Yung Sung jIANG Ren-Wang Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization Guangxi Institute of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research College of Pharmacy Jinan University State Key Laboratory of New Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Department of Chemistry The Hong Kong University of Science and Technology

Podophyllotoxone(1) was isolated from the roots of Dysosma versipellis. The structure was determined by spectroscopic analysis in combination with single-crystal X-ray analysis. The absolute configuration of compound 1 was assigned based on the Flack parameter. It showed significant inhibitory activities against human prostate cancer cells PC3 and DU145 with IC50 values being 14.7 and 20.6 μmol·L-1, respectively. It also arrested the cells at G2/M phase. Tubulin polymerization assay showed that it inhibited the tubulin polymerization in a dose-dependent manner, and molecular docking analysis revealed a different binding mode with tubulin as compared with those known tubulin inhibitors.

关键词： Podophyllotoxone Absolute configuration Prostate cancer Tubulin Molecular docking

Synthesis of Isotopically-Labeled Graphite Films by Cold-Wall Chemical Vapor Deposition and Electronic Properties of Graphene Obtained from Such Films

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Nano Research 2009年第11期2卷 851-856页

作者： Weiwei Cai Richard D.Piner Yanwu Zhu Xuesong Li Zhenbing Tan herman Carlo Floresca Changli Yang Li Lu M.j.Kim Rodney S.Ruoff Department of Mechanical Engineering and the Texas Materials Institute University of Texas at AustinAustinTX 78712USA Institute of Physics Chinese Academy of SciencesBeijing 100190China Department of Materials Science and Engineering University of Texas at DallasRichardsonTX 75083USA

We report the synthesis of isotopically-labeled graphite films on nickel substrates by using cold-wall chemical vapor deposition(CVD).During the synthesis,carbon from^(12)C-and^(13)C-methane was deposited on,and dissolved in,a nickel foil at high temperature,and a uniform graphite film was segregated from the nickel surface by cooling the sample to room *** and transmission electron microscopy,micro-Raman spectroscopy,and X-ray diffraction prove the presence of a graphite *** graphene films obtained from such isotopically-labeled graphite films by mechanical methods have electron mobility values greater than 5000 cm^(2)·V^(-1)·s^(-1)at low ***,such films exhibit the half-integer quantum Hall effect over a wide temperature range from 2 K to 200 K,implying that the graphite grown by this cold-wall CVD approach has a quality as high as highly oriented pyrolytic graphite(HOPG).The results from transport measurements indicate that^(13)C-labeling does not significantly affect the electrical transport properties of graphene.

关键词： Chemical vapor deposition(CVD) isotopically-labeled graphite graphene

Comparison of Lattice Boltzmann,Finite Element and Volume of Fluid Multicomponent Methods for Microfluidic Flow Problems and the jetting of Microdroplets

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Communications in Computational Physics 2023年第3期33卷 912-936页

作者： Karun P.N.Datadien Gianluca Di Staso Christian Diddens herman M.A.Wijshoff Federico Toschi Department of Applied Physics and Science Education Eindhoven University of Technology5600MB EindhovenNetherlands FLOW Matters Consultancy B.V. 5612AEEindhovenThe Netherlands Physics of Fluids group Department of Science and TechnologyMesa+InstituteMax Planck Center for Complex Fluid Dynamics and J.M.Burgers Centre for Fluid DynamicsUniversity of TwenteP.O.Box 2177500 AE EnschedeThe Netherlands Canon Production Printing Netherlands B.V. 5914 HHVenloThe Netherlands Department of Mathematics and Computer Science Eindhoven University of Technology5600MB EindhovenNetherlands Istituto per le Applicazioni del Calcolo Consiglio Nazionale delle Ricerche00185 RomeItaly.

We show that the lattice Boltzmann method(LBM)based color-gradient model with a central moments formulation(CG-CM)is capable of accurately simulating the droplet-on-demand inkjetting process on a micrometer length scale by comparing it to the Arbitrary Lagrangian Eulerian Finite Element Method(ALE-FEM).A full jetting cycle is simulated using both CG-CM and ALE-FEMand results are quantitatively compared by measuring the ejected ink velocity,volume and contraction *** also show that the individual relevant physical phenomena are accurately captured by considering three test-cases;droplet oscillation,ligament contraction and capillary *** first two cases test accuracy for a dynamic system where surface tension is the driving force and the third case is designed to test wetting boundary *** the first two cases we also compare the CG-CM and ALE-FEM results to Volume of Fluid(VOF)*** of the three methods shows close agreement when compared to each other and analytical solutions,where *** we demonstrate that asymmetric jetting is achievable using 3D CG-CM simulations utilizing asymmetric wetting conditions inside the *** allows for systematic investigation into the physics of asymmetric jetting,*** to jet-nozzle manufacturing imperfections or due to other disturbances.

关键词： Lattice Boltzmann method multiphase flow multicomponent flow inkjet printing

Pathogenic Variation and Occurrence of Multiple Resistance-Breaking <i>Rice yellow mottle virus</i>Strains in Tanzania

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American journal of Plant Sciences 2017年第8期8卷 1820-1841页

作者： judith Hubert herman j. F. Lyimo Ashura Luzi-Kihupi Department of Crop Science and Horticulture Sokoine University of Agriculture Morogoro Tanzania

Rice yellow mottle virus (RYMV) is a major biotic constraint for rice production in Africa. The resistance-breaking ability of Tanzanian RYMV strains and phylotypes (S4lm (Tz526), S4lv (Tz516), S4ug (Tz508), S5 (Tz429, Tz445), S6c (Tz486) and S6w (Tz539)) were tested by inoculating rice cultivars with RYMV1 resistant alleles (Gigante (rymv1-2), Tog12387 (rymv1-3), Tog5681 (rymv1-3), Tog5438 (rymv1-4), Tog5672 (rymv1-4+rymv2) and Tog5674 (rymv 1-5)) in a screen house. The results revealed multiple resistance-breaking strains and phylotypes on resistant cultivars Gigante, Tog12387, Tog5438 and Tog5681. However, the resistance breakdown was highly variable depending on the strain used, and disease severity ranged from 11% - 75.3%. The virulence potential of RYMV phylotype S4lm (Tz526) was similar to phylotype S6w (Tz539). The impact of strains and phylotypes on yield and its components in rice cultivars revealed highly significant differences (P ≤ 0.001). The lowest percent plant height reduction (2.8%), number of tillers per plant (2.5%), 1000 grain weight (2.7%), spikelet sterility (3.5%) and yield (5%) was recorded in rice cultivar Gigante inoculated with RYMV phylotype S6c (Tz486). Phylotype S6c (Tz486) despite being less virulent compared to other strains, its virus titer in rice cultivar Gigante (1.833) was higher than S5 (Tz429, Tz445) inoculated on Tog5674 (0.171, 0.207) and S6w (Tz539) inoculated on Tog5681 (0.283). The resistant-breaking strain S5 (Tz445) multiplied in resistant rice cultivar Tog5674 without inducing visible symptoms but showed positive reaction to ELISA with low virus titer. The strain S5 overcame wide range of resistant alleles including rymv1-2, rymv1-3, rymv1-4 and rymv1-5 resistance, with exception of rymv1-4 + rymv2. The current results gave a new perspective for future identification of resistance-breaking mutations through sequencing of the RYMV genome in infected rice cultivars and mutagenesis of an infectious viral clone useful for

关键词： Pathogenic Variation Virulence Multiple Resistance-Breaking RYMV Strains Yield Losses Tanzania

Geographical Variation, Distribution and Diversity of <i>Rice Yellow Mottle Virus</i>Phylotypes in Tanzania

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American journal of Plant Sciences 2017年第6期8卷 1264-1284页

作者： judith Hubert herman j. F. Lyimo Ashura Luzi-Kihupi Department of Crop Science and Production Sokoine University of Agriculture Morogoro Tanzania

Rice yellow mottle virus (RYMV) is the most important disease of rice in Africa. The disease was first observed in1966 inKenya but has now spread in all rice-growing countries of Sub-Saharan Africa. In Tanzania, its distribution has been restricted to the major rice-growing regions. However, the knowledge on RYMV genetic diversity relies on a limited number of coat protein sequences. Previous studies revealed the presence of the phylotypes S4lv, S4lm and strain S5 in Mwanza, Mbeya and Morogoro regions, respectively, and strain S6 in Kilimanjaro region and Pemba Island. Surveys were conducted during the cropping seasons 2013-2014 in eight rice-growing regions of Tanzania to determine geographical variations and phylotypes of RYMV and the influence of environment factors on its distribution and diversity. A total of 185 rice fields were surveyed. Results indicate that prevalence, severity and phylotypes of RYMV varied significantly with rainfall intensity, temperature and relative humidity (P ≤ 0.01). The highest prevalence was found in Morogoro (82%), Mbeya (80%) and Arusha (67.33%) regions whereas Kigoma (9.33%), Rukwa (11.33%) and Shinyanga (18.67%) had the lowest RYMV prevalence. In each region, RYMV prevalence was higher in 2014 than in 2013. The phylotypes S4lm and new determined phylotypes (S6c and S6w) were highly adapted to low temperature (13.3°C) and rainfall (13.7 mm) areas. For the first time, strains from the phylotype S4ug were found outside Uganda, in Kilimanjaro region. Strain S4lv (phylotype Lake Victoria) was found for the first time in Arusha region. The strain S4lm was found in Mbeya, Morogoro and Rukwa regions. Strains S4lm and S4lv were also found in Shinyanga and Kigoma regions, respectively. The strain S5 was still restricted to Morogoro but extended to new locations such as Ulanga district. Strain S6 was found in several new areas and new phylotypes of S6 (S6c and S6w) are reported in this study.

关键词： Rice Yellow Mottle Virus Prevalence Severity Environmental Factors Tanzania