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Development and application of functional gene arrays for microbial community analysis

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中国有色金属学会会刊：英文版 2008年第6期18卷 1319-1327页

作者： z. L. HE j. D. VAN NOSTRAND L.Y. wu j. z. z.OU Institute for Environmental Genomics Department of Botany and Microbiology University of Oklahoma Norman OK 73019 USA

功能的基因标记能关于功能的基因差异和微生物引起的社区的潜在的活动提供重要信息。尽管 microarray 技术成功地被使用了为纯文化学习基因表示，简单、人工的微生物引起的社区，改编如此的一种技术分析复杂微生物引起的社区仍然以设计... 详细信息

功能的基因标记能关于功能的基因差异和微生物引起的社区的潜在的活动提供重要信息。尽管 microarray 技术成功地被使用了为纯文化学习基因表示，简单、人工的微生物引起的社区，改编如此的一种技术分析复杂微生物引起的社区仍然以设计，样品准备，和数据分析提出很多挑战。这个工作集中于功能的基因数组(FGA ) 的开发和应用程序为微生物引起的社区研究指向关键功能的基因标记。一些与 FGA 有关明确地给问题调音，例如 oligonucleotide 探查设计， nucleic 酸抽取和纯化，数据分析，特性，敏感，和量的能力详细被讨论。最近的研究证明了 FGA 能从许多自然环境关于微生物引起的社区提供特定、敏感、潜在地量的信息并且控制生态系统。这种技术被期望革命化微生物引起的社区的分析，并且连接微生物引起的结构到生态系统工作。

关键词：微数列应用函数基因标记微生物种群

Numerical investigation on the flowfield of “swallowtail” cavity for supersonic mixing enhancement

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Acta Mechanica Sinica 2009年第1期25卷 37-44页

作者： C. Wang z. j.ang z. Hu j. Lu Institute or Mechanics. Chinese Academy of Sciences 100190 Beijing China ReCAPT Gyeongsang National University JinjuKyeongnam 660-701 South Korea

A ＂swallowtail＂ cavity for the supersonic combustor was proposed to serve as an efficient flame holder for scramj.ts by enhancing the mass exchange between the cavity and the main flow. A numerical study on the ＂swallow- tail＂ cavity was conducted by solving the three-dimensional Reynolds-averaged Navier-Stokes equations implemented with a k-e turbulence model in a multi-block mesh. Turbu- lence model and numerical algorithms were validated first, and then test cases were calculated to investigate into the mechanism of cavity flows. Numerical results demonstrated that the certain mass in the supersonic main flow was sucked into the cavity and moved spirally toward the combustor walls. After that, the flow went out of the cavity at its lateral end, and finally was efficiently mixed with the main flow. The comparison between the ＂swallowtail＂ cavity and the conventional one showed that the mass exchanged between the cavity and the main flow was enhanced by the lateral flow that was induced due to the pressure gradient inside the cavity and was driven by the three-dimensional vortex ring generated from the ＂swallowtail＂ cavity structure.

关键词： Supersonic mixing - Flame holder "Swallowtail" cavity - Combustion ignition Numerical simulation

HOT DEFORMATION AND MARTENSITIC TRANSFORMATION BEHAVIORS OF Fe-32%Ni ALLOY

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Acta Metallurgica Sinica(English Letters) 2006年第2期19卷 133-138页

作者： j. Huang z. Xu Cold Rolling Mill Baoshan Iron ＆ Steel Co. Ltd Shanghai 201900 China Key Laboratory for High Temperature Materials and Tests of Ministry of Education Shanghai Jiaotong University Shanghai 200030 China

The Hot deformation and martensitic transformation behaviors of Fe-32%Ni alloy was investigated by measurements of electrical resistance and X-ray diffraction. With the increase in strain, the austenite goes through from the work-hardened to the partial dynamcally re-crystalliz.d and then to the completed dynamically re-crystalliz.d. The martensitic transformation characteristics depend on the austenite states. The work-hardening in small strain is helpful to martensitic transformation due to the low dislocation density and little lattice distortion, while the high dislocation density and severe lattice distortion by the increase in strain will hinder the martensitic nucleation. Once dynamic re-crystalliz.tion （ DRX ） takes place, the martensitic transformation will be enhanced again, which is related to the heterogeneous dynamic substructures. The growing DRX grain can enhance the martensitic nucleation due to the low dislocation density near its grain boundary.

关键词： Fe-32%Ni alloy hot deformation work hardening dynamic re-crystalliz.tion martensitic transformation

Decoloriz.tion of Direct Black 22 by Aspergillus ficuum

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j.urnal of Environmental Sciences 2001年第4期13卷 472-475页

作者： Dong, X.-j. Du, z.-y. Chen, z. Dept. of Biology/Environmental Sci. Wenzhou Normal College Wenzhou 325003 China

The decoloriz.tion of Direct Black 22 by Aspergillus ficuum has been studied. It was found that Aspergillus ficuum could effectively decoloriz. Direct Black 22 especially when grown as pelleted mycelia. Results showed that the media containing Direct Black 22 at 50 mg/L could be decoloriz.d by 98.05% of the initial color in 24 h. The optimum pH and temperature of decoloriz.tion are 4.0 and 33 °C respectively. Aeration was quite beneficial to decoloriz.tion. Medium composition and the concentration of Direct Black 22 could affect the rate of decoloriz.tion. The dye degraded products assayed by UV-visible spectrophotometer and macroscopic observation showed that the decoloriz.tion of Direct Black 22 by mycelial pellets includes two important processes: bioadsorption and biodegradation. The degradation experiment agree with the Michaelis-Menten kinetics equation.

关键词： Bioassay Biodegradation Color Concentration (process) Dyes pH Ultraviolet spectrophotometers

Large pore siz. polyacrylonitrile membrane for ultrafiltration

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j.urnal of Environmental Sciences 2000年第4期12卷 478-481页

作者： wu, K. Qiu, P. Wang, z. Wang, j. Rsrch. Center for Eco-Envtl. Sci. Chinese Academy of Sciences Beijing 100085 China

The effects of the components of solution for membrane casting and preparation conditions on the membrane performances are studied in this paper. Polyacrylonitrile (PAN) was used as polymer and DMAC as solvent. The ultrafiltration (UF) membranes whose cut-off of molecular weight is 150000 and flux of pure water reaches 150-200 ml/(cm2·h) were prepared by selecting proper components of solution for membrane casting and membrane preparation conditions.

关键词： Casting Molecular weight Polyacrylonitriles Pore siz. Water quality

Large eddy simulation of turbulent diffusion flame combustion using a conserved scalar methodology

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航空动力学报 2007年第7期22卷 1106-1117页

作者： K. Wang z. Yang j. j. McGuirk Department of Aeronautical and Automotive EngineeringLoughborough University Loughborough Leicestershire LE11 3TU UK

The present paper describes an LES prediction of turbulent diffusion flame combustion in a simplifled axi-symmetric combustor *** calculations are carried out using a well-tested finite volume incompressible LES code which has been modified to handle variable density and reacting *** basic mixture fraction conserved scalar method *** with the chemical state relationships described by fast *** turbulence-chemistry interaction is modelled by a sub-grid PDF method and the PDF is assumed to follow a Beta-function *** LES predictions have been time-averaged over 3.5 flow-through times to generate the mean radial profiles of mixture fraction,product mass fraction,temperature,axial velocity and axial *** agreement of the LES predictions with the experimental data is good for all the above quantities at four different axial positions with largest differences at the first measurement *** LES method also provides information on the unsteady nature of turbulent diffusion *** turbulent reacting flows with large density ratio,it was found necessary to use a relaxation method in order to remove unphysical high-frequency fluctuations and to maintain numerical stability.

关键词：大涡模拟湍流扩散数量方法论弛豫法燃烧理论

EFFECT OF AUSTEMPERING ON TRANSFORMATION INDUCED PLASTICITY OF HOT ROLLED MULTIPHASE STEELS

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Acta Metallurgica Sinica(English Letters) 2007年第1期20卷 1-8页

作者： z. Li D. wu The State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 China Shenyang Institute of Aeronautical Engineering Shenyang 110034 China

Effect of austempering on the transformation induced plasticity （TRIP） of hot rolled multiphase steel was investigated. Polygonal ferrite, granular bainite, and a large amount of stabiliz.d retained austenite could be obtained in the hot rolled multiphase steel. Strain induced martensite transformation （SIMT） of retained austenite and TRIP effectively occur under straining owing to austempering after hot rolling, and mechanical properties of the present steel remain at a relatively high constant value for austempering at 400℃. The mechanical properties of the steel exhibited a good combination of tensile strength （791MPa） and total elongation （36%） because the stability of retained austenite is optimal when the steel is held for 20min.

关键词： austempering hot rolled multiphase steel mechanical property strain-induced transformation transformation-induced plasticity (TRIP)

The j.int Control of Variable Speed and On-Ramp Metering for Urban Expressway

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j.urnal of Systems Engineering and Electronics 2001年第3期12卷 25-30页

作者： Ma, j. Liu, X. Rong, j. Li, z. Transportation Research Center Beijing Polytechnic University Beijing 100022 China

The traffic performance of urban expressway is subj.ct to non-recurring and recurring events, which may cause heavy congestion and vehicles long queuing on ramps. The low performance may bring more traffic delay to the whole network of urban road. This paper presents a new method, the j.int control of variable speed control and on-ramp metering, which attempts to improve the level of traffic operations on urban expressway. By analyz.ng traffic flow on urban expressway, an optimum control strategy of variable speed and on-ramp metering is established in the paper.

关键词： Function evaluation Highway traffic control Intelligent control Mathematical models Motor transportation Parameter estimation Ramp generators Speed control

A novel process for sorting fine-siz.d sulphide minerals by biocoagulation

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中国有色金属学会会刊：英文版 2008年第6期18卷 1473-1479页

作者： H. z. KUYUMCU j. PINKA T. BIELIG Department of Mechanical Process Engineering and Solids Processing Technical University of Berlin Sekr. BH 11 Strasse des 17. Juni 135 10623 Berlin Germany

基于一个过程设计想法，在柏林的技术大学的调查证实微生物的 biocoagulation 和稳固的粒子将是一个新方法产生对排序合适的更粗糙的粒子。微生物的选择 biocoagulation 的过程，分别地例如酵母喜欢 Saccharomyces cerevisiae 和 Yarrow... 详细信息

基于一个过程设计想法，在柏林的技术大学的调查证实微生物的 biocoagulation 和稳固的粒子将是一个新方法产生对排序合适的更粗糙的粒子。微生物的选择 biocoagulation 的过程，分别地例如酵母喜欢 Saccharomyces cerevisiae 和 Yarrowia lipolytica，并且例如象方铅矿和闪锌矿一样的矿物质，微分散固体作为一个新奇排序过程的一个基础被分析了。因此，特别微生物的房间表面的特征例如静电的费用和细胞外的聚合物质的作文，以及他们对选择 biocoagulation 的影响被学习。在 10 m 下面的微生物和硫醚粒子有效地凝结的试验性的调查表演。而且，筹款对有选择地形成的 biocoagulates 的分离合适。与设计的列筹款，令人满意的恢复率被到达。

关键词：硫矿拣选生物凝固微生物