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EB病毒基因组限制片段长度多态性(RFLP)在鼻咽组织中的分布

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中华微生物学和免疫学杂志 1995年第6期15卷 415-419页

作者：陈鸿霖宗永生 j.sham D.Choy 吴文翰广东省汕头大学生物系 515063 中山医科大学病理教研室香港玛丽医院放射治疗部香港大学微生物系

利用PCR和限制性内切酶分析,探讨了EB病毒基因组限制片段长度多态型(RFLP)在正常鼻咽组织,鼻咽癌组织和口腔上皮脱落细胞中的分布情况。结果表明在正常鼻咽组织的EB病毒感染是一种普遍现象并且感染可能发生在癌变前;EB病毒基因组BamHF区... 详细信息

利用PCR和限制性内切酶分析,探讨了EB病毒基因组限制片段长度多态型(RFLP)在正常鼻咽组织,鼻咽癌组织和口腔上皮脱落细胞中的分布情况。结果表明在正常鼻咽组织的EB病毒感染是一种普遍现象并且感染可能发生在癌变前;EB病毒基因组BamHF区的RFLP在鼻咽癌组织和正常的鼻咽组织之间有不同的分布;不同EB病毒RFLP变型(variant)的混合感染在正常咽组织比在癌组织中普遍,这结果表明可能在癌变发展过程中,由于部分带有EB病毒的细胞的选择生长,结果使得癌组织中的细胞以一种EB病毒变型为主;本文探讨了EB病毒的RFLP变型在鼻咽组织和口腔上皮脱落细胞分布的关系,这种关系可能为鼻咽癌高发区的癌变检测提供一种简单可靠的方法。

关键词： EB病毒鼻咽癌口腔上皮脱落细胞

Serological diagnosis of nasopharyngeal carcinoma by enzyme linked immunosorbant assay:optimization,standardization and diagnostic criteria

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Chinese Medical journal 1998年第6期000卷 51-56页

作者： M.H. Ng, H.L. Chen, R.X. Luo, K.H. Chan, P.C.Y. Woo, j.S.T. sham, j. Huang, W.H.Seto P. Smith and B.E. Griffin Department of Microbiology The University of Hong Kong Hong Kong China Department of Biology Shantou University Shantou China Department of Microbiology Queen Mary Hospital Hong Kong China Department of Microbiology and Department of Radiation Oncology The University of Hong Kong Hong Kong China Department of Virology Royal Postgraduate Medical School University of London London United Kingdom

Objectives To produce an enzyme linked immunosorbant assay (ELISA) to detect antibodies against Epstein Barr virus (EBV) specified nuclear antigen 1 (EBNA 1) and the 18kD EBV matrix protein, and to determine and optimize its sensitivity and specificity for the diagnosis of nasopharyngeal carcinoma (NPC).Methods We used a combination of highly purified glutathione transferase fusion proteins of the 40kD carboxy domain of EBNA1 and the 18kD EBV matrix protein for coating ELISA plates. In three separate studies, we tested for IgA antibodies in serum specimens from 28 EBV seronegative donors, 284 EBV seropositive donors and 160 newly diagnosed NPC patients. By comparing the sensitivity and specificity of diagnosis obtained for different cutoff values, we derived several quantitative parameters to evaluate assay performance, establish objective diagnostic criteria which optimize the intrinsic diagnostic capability of the assay and assess the significance of individual test results, respectively. Optimum cutoff optical density (OD) is defined as the cutoff OD where sensitivity of the assay equals its specificity, and resolution of the assay is indicated by the value of sensitivity (or specificity) determined at the optimum cutoff OD. Diagnosis of NPC was achieved by setting a cutoff zone at +/-20% of this *** All the EBV seronegative donors tested were not reactive, and most of the EBV seropositive donors were weakly reactive, while the majority of NPC patients were moderately or strongly reactive. While the assay was thus shown to be specific for EBV, there was an overlap in the level of these serum antibodies between few individuals of the two latter groups. It was shown that the assay performed equally well in two separate studies conducted under different testing conditions and using different collections of sera in that assay resolution determined on these occasions were 86% and 87% respectively. Diagnosis of NPC can be achieved at the same expected sensiti

关键词： Serological diagnosis of nasopharyngeal carcinoma by enzyme linked immunosorbant assay

Potent antitumor efficacy of an E1B 55kDa-deficient adenovirus carrying murine endostatin in hepatocellular carcinoma

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第二军医大学学报 2005年第11期26卷 1330-1330页

作者： Li G sham j Yang j Su C Xue H Chua D Sun L Zhang Q Cui Z Wu M Qian Q Laboratory of Viral and Gene Therapy Eastern Hepatobiliary Surgery HospitalSecond Military Medical University Shanghai 200438 China

Potent antitumor efficacy of an E1B 55 kDa-deficient adenovirus carrying murine endostatin in hepatocellular carcinoma

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第二军医大学学报 2006年第4期27卷 423-423页

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光控耦合双量子点之间的超快相位门

光控耦合双量子点之间的超快相位门

Potent antitumoral efficacy of a novel replicative adenovirus CNHK300 targeting telomerase-positive cancer cells

第十五届全国量子光学学术报告会

作者：陈立波 L.j.sham EdoWaks 顾永建中国海洋大学物理系 Center for Advanced Nanoscience Department of PhysicsUniversity of California-San Diego 青岛理工大学理学院 joint Quantum Institute University of Maryland and National Institute of Standards and TechnologyCollege Park

＜正＞在本文中我们利用空穴隧道效应(取代文献中的电子隧道效应)来实现这类垂直生长的双量子点之间的相位门。和文献相比我们的方案中两个量子点之间的电子初始时是没有相互作用

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来源： cnki会议评论

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第二军医大学学报 2006年第1期27卷 75-75页

作者： Su CQ sham j Xue HB Wang XH Chua D Cui ZF Peng LH Li LF jiang LH Wu MC Qian Qj Laboratory of Viral and Gene Therapy Eastern Hepatobiliary Surgical Hospital Second Military Medical University Shanghai 200438 China

Effective gene-viral therapy for telomerase-positive cancers by selective replicative-competent adenovirus combining with endostatin gene

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第二军医大学学报 2005年第2期26卷 194-194页

作者： Zhang Q Nie M sham j Su C Xue H Chua D Wang W Cui Z liu Y Liu C jiang M Fang G Liu X Wu M Qian Q Laboratory of Viral and Gene Therapy Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

Gene-viral therapy, which uses replication-selective transgene-expressing viruses to manage tumors, can exploit the virtues of gene therapy and virotherapy and overcome the limitations of conventional gene therapy. Using a human telomerase reverse transcriptase-targeted replicative adenovirus as an antiangiogenic gene transfer vector to target new angiogenesis and making use of its unrestrained proliferation are completely new concepts in tumor management. CNHK300-mE is a selective replication transgene-expressing adenovirus constructed to carry mouse endostatin gene therapeutically. Infection with CNHK300-mE was associated with selective replication of the adenovirus and production of mouse endostatin in telomerase-positive cancer cells. Endostatin secreted from a human gastric cell line, SGC-7901, infected with CNHK300-mE was significantly higher than that infected with nonreplicative adenovirus Ad-mE in vitro (800±94.7 ng/ml versus 132.9±9.9 ng/ml) and in vivo (610±42 ng/ml versus 126 +/- 13 ng/ml). Embryonic chorioallantoic membrane assay showed that the mouse endostatin secreted by CNHK300-mE inhibited angiogenesis efficiently and also induced distortion of pre-existing vasculature. CNHK300-mE exhibited a superior suppression of xenografts in nude mice compared with CNHK300 and Ad-mE. In summary, we provided a more efficient gene-viral therapy strategy by combining oncolysis with antiangiogenesis.

关键词： gene therapy replicative adenovirus gene transfer nude mice cell line in vitro

Attenuation of mitochondrial,but not cytosolic,Ca2+ overload reduces myocardial injury induced by ischemia and reperfusion

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Acta Pharmacologica Sinica 2006年第7期27卷 911-918页

作者： Kenneth WL KAM james SK sham Tlak-ming WONG Department of Physiology the University of Hong KongHong Kong SARChina Division of Pulmonary Critical Care Medicine Johns Hopkins School of MedicineBaltimoreMaryland 21224USA

Aim:Attenuation of mitochondrial Ca2+([Ca2+]m),but not cytosolic Ca2+([Ca2+]c),overload improves contractile *** hypothesized that attenuation of[Ca2+]m,but not[Ca2+]c,overload confers cardioprotection against ischemia/reperfusion-induced ***:Infarct size from isolated perfused rat heart,cell viability,and electrically-induced Ca2+transient in isolated rat ventricularmyocytes were *** determined the effects of BAPTA-AM,a Ca2+chelator,at concentrations that abolish the overload of both[Ca2+]cand[Ca2+]m,and ruthenium red,an inhibitor of mitochondrial uniporter of Ca2+transport,atconcentrations that abolish the overload of[Ca2+]m,but not[Ca2+]c,on cardiacinjury induced by ischemia/***:Attenuation of both[Ca2+]mand[Ca2+]cby BAPTA-AM,and attenuation of[Ca2+]m,but not[Ca2+]c,overloadby ruthenium red,reduced the cardiac injury observations,indicating the impor-tance of[Ca2+]min cardioprotection and contractile recovery in response to is-chemia/***:The study has provided unequivocal evidenceusing a cause-effect approach that attenuation of[Ca2+]m,but not[Ca2+]c,over-load is responsible for cardioprotection against ischemia/*** also confirmed the previous observation that attenuation of[Ca2+]m,but not[Ca2+]c,by ruthenium red improves contractile recovery following ischemia/reperfusion.

关键词： ruthenium red BAPTA-AM infarct size electrically-induced Ca2+transient isolated perfused rat heart cardiac myocytes

Statistical issues and approaches in endophenotype research

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Chinese Science Bulletin 2011年第32期56卷 3403-3408页

作者： sham Pak Chung CHERNY Stacey S HALL Mei-Hua Department of Psychiatry and The State Key Laboratory of Brain and Cognitive Sciences The University of Hong Kong Hong Kong China Psychology Research Laboratory McLean Hospital Harvard Medical School Boston MA 02115-6092 USA

The endophenotype concept was initially proposed to enhance the power of genetic studies of complex disorders. It is closely related to the genetic component in a liability-threshold model; a perfect endophenotype should have a correlation of 1 with the genetic component of the liability to disease. In reality, a putative endophenotype is unlikely to be a perfect representation of the genetic component of disease liability. The magnitude of the correlation between a putative endophenotype and the genetic component of disease liability can be estimated by fitting multivariate genetic models to twin data. A number of statistical methods have been developed for incorporating endophenotypes in genetic linkage and association analyses with the aim of improving statistical power. The most recent of such methods can handle multiple endophenotypes simultaneously for the greatest increase in power. In addition to increasing statistical power, endophenotype research plays an important role in helping to understand the mechanisms which connect the associated genetic variants with disease occurrence. Novel statistical approaches may be required for the analysis of the complex relationships between endophenotypes at different levels and how they converge to cause the occurrence of disease.

关键词：统计问题稳定遗传疾病 COM组件复杂疾病组成部分模型估计统计方法