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Open Journal of Stomatology 2014年第5期4卷 249-257页

作者： isao tamura Aiko Kamada Seiji Goda Yoshihiro Yoshikawa Eisuke Domae Takashi Ikeo Department of Biochemistry Osaka Dental University Hirakata Japan

In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human buccal mucosa squamous cancer cell line BICR 10 treated with 3 nM tyrphostin. Growth of BICR 10 cells was inhibited by treatment with tyrphostin. Although changes were not observed in the expression of EGFR and p120-catenin, expression of Akt, Src and periplakin in BICR 10 treated with 3 nM tyrphostin tended to decrease. In addition, phosphorylation of EGFR, Akt and Src was inhibited by treatment with tyrphostin. On immunocytochemical staining, immunoreactions with phosphorylated EGFR, phosphorylated Akt and phosphorylated p120-catenin were weak in BICR 10 treated with tyrphostin. There was a slight immunocy to chemical reaction to periplakin in BICR 10 cells induced by tyrphostin. In conclusion, the decrease in phosphorylation in EGFR and p120-catenin by tyrphostin, following the decrease in Src or Akt phosphorylation, may inhibit expression of several growth factors associated with the proliferation and migration of cancer cells.

关键词： 4-(3-Chloro-Benzyl)-6 7-Dimethoxy-Quinazoline Human Buccal Mucosa Squamous Cancer Cell Line P120-Catenin Periplakin

Influence of Adiponectin and Leptin on the Kinetics of Human Pulp Cells

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Open Journal of Stomatology 2020年第12期10卷 360-371页

作者： isao tamura Katsura Ueda Yoshifumi Matsuda Aiko Kamada Yoshihiro Yoshikawa Eisuke Domae Takashi Ikeo Shunji Kumabe Department of Oral Anatomy School of Dentistry Osaka Dental University 8-1 Kuzuhahanazono-cho Hirakata-shi Osaka Japan Department of Biochemistry School of Dentistry Osaka Dental University 8-1 Kuzuhahanazono-cho Hirakata-shi Osaka Japan

Adipocytokines, such as adiponectin and leptin, are expressed by adipocytes and are known as anti-metabolic syndrome factors. They are also thought to mediate calcification in mineralized tissue. We investigated the effects of adiponectin and leptin on the kinetics of human pulp cells using ELISA and western blot. After gaining informed consent, we obtained human pulp cells from three patients. After cultivation in Dulbecco’s Modified Eagle Medium (DMEM) without serum, cells from the 4th to 6th passages were incubated with various concentrations of adiponectin (1, 10, or 100 ng/mL) in DMEM or leptin (0.1, 1, or 10 ng/mL) in DMEM for 24 h. We confirmed that human pulp cells expressed adiponectin receptor 1 and leptin receptor. Although the proliferation index of these cells as measured by 5-bromo-2'-deoxyuridine (BrdU) incorporation increased in the presence of adiponectin in a dose-dependent manner, pulp cells stimulated with leptin showed no significant changes in BrdU incorporation. Alkaline phosphatase activity showed no significant changes after stimulation with either adipocytokine. Adiponectin induced expression of BMP-2 and osteopontin more strongly than did leptin. In particular, expression of BMP-2 increased in the presence of adiponectin in a dose-dependent manner. In contrast, the expression of dentin sialoprotein increased after stimulation with leptin. The expression of Runx2 was not observed from the cultured pulp cells stimulated with both molecules. These results indicate that adiponectin and leptin contribute to growth and differentiation of human pulp cells and may consequently affect the formation of secondary dentin or reparative dentin in the dentin-pulp complex.

关键词： Dental Pulp Cells Adiponectin Leptin Bone Morphogenetic Protein-2 Dentin Sialoprotein Osteopontin ECM Formation

Expression of Plectin-1 and Trichohyalin in Human Tongue Cancer Cells

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Open Journal of Stomatology 2018年第6期8卷 196-204页

作者： isao tamura Katsura Ueda Tetsunari Nishikawa Aiko Kamada Tomoharu Okamura Yoshifumi Matsuda Kentaro Ueno Yoshihiro Yoshikawa Eisuke Domae Kazuya Tominaga Shunji Kumabe Takashi Ikeo Akio Tanaka Department of Oral Anatomy School of Dentistry Osaka Dental University Hirakata Japan Department of Innovation in Dental Education School of Dentistry Osaka Dental University Hirakata Japan Department of Biochemistry School of Dentistry Osaka Dental University Hirakata Japan Department of Oral Pathology School of Dentistry Osaka Dental University Hirakata Japan Department of Pathology School of Dentistry Osaka Dental University Hirakata Japan

In basal squamous cells, plectin-1 interacts with intermediate filaments, whereas trichohyalin, which is distributed primarily in the medulla and inner root sheath cells of human hair follicles, plays a role in strengthening cells during keratinization. Although both cytoskeletal proteins occur in trace amounts in human tongue epithelial cells, there are minimal data on their expression in human tongue primary cancer cells. We therefore investigated the expression of plectin-1 and trichohyalin in human tongue epithelial cell line (DOK) and tongue cancer cell line (BICR31) using western blotting and FITC-labeled immunocytochemistry techniques. DOK and BICR31 cells were cultivated to subconfluence in Dulbecco’s Modified Eagle’s Medium containing 0.4 μg/ml of hydrocortisone and 10% fetal bovine serum, and the levels of trichohyalin and plectin-1 were determined by western blot analysis and immunocytochemical staining. Trichohyalin expression was clearly observed, with no differences between DOK and BICR31 cells. Although DOK cells expressed trace levels of plectin-1, obvious plectin-1 bands were detected in western blot analyses of BICR31 cells. Immunocytochemical staining revealed that trichohyalin and plectin-1 localize in the cytoplasm. Trichohyalin was diffusely distributed in both cell lines, and colocalization of trichohyalin and cytokeratin 1/10 was observed in almost all BICR31 cells. There were no correlations between western blot and immunocytochemical data for trichohyalin. Conversely, correlations in immunochemical reactions for plectin-1 were observed. Most DOK cells showed no localization of plectin-1, but strong reactions were detected in the cytoplasm of BICR31 cells. These results indicate that trichohyalin is expressed by cancerous tongue epithelial cells during various stages of malignancy and that plectin-1 provides an index of malignancy.

关键词： Tongue Cancer Plectin-1 Trichohyalin Diagnosis

Expression of the Japanese oak silkworm Antheraea yamamai fibroin gene in the domesticated silkworm Bombyx mori

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Insect Science 2009年第6期16卷 465-473页

作者： isao Kobayashi Katsura Kojima Hideki Sezutsu Keiro Uchino Toshiki tamura Transgenic Silkworm Research Center Silk Materials Research Unit National Institute of Agrobiological Sciences Ibaraki Japan

To understand the evolutionary conservation ofthe gene expression mechanism and secretion machinery between Antheraea and Bombyx fibroins, we introduced the genomic A. yamamai fibroin gene into the domesticated silkworm, B. mori. The spliced A. yamamai fibroin mRNA appeared only in the posterior region of the silk gland of the transgenic silkworm, suggesting that the functions of the fibroin promoter region and the splicing machinery are conserved between these two species. The A. yamarnai fibroin protein was detected in the lumen of the silk gland of the transgenic silkworm, albeit at lower levels compared with the B. mori-type fibroin. We found a strong degeneration of the posterior region of the silk gland of the transgenic silkworm. As a result, the cocoon shell weight was much lower in the transgenic silkworm than in the non-transgenic line. These results indicate that the promoter function and splicing machinery are well conserved between A. yamamai and B. mori but that the secretion mechanism of fibroin is diversified between the two.

关键词： Antheraea yamamai Bombyx mori evolution fibroin silk transgenic

Diagnosis and management of pancreatic neuroendocrine tumor in von Hippel-Lindau disease

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World Journal of Gastroenterology 2010年第36期16卷 4515-4518页

作者： Kenji tamura isao Nishimori Tetsuhide Ito Ichiro Yamasaki Hisato Igarashi Taro Shuin Department of Urology Kochi Medical SchoolNankokuKochi 783-8505Japan Nishimori's Clinic SakawaKochi 789-1233Japan Department of Medicine and Bioregulatory Science Graduate School of Medical SciencesKyushu UniversityHigashi-kuFukuoka 812-8582Japan

The pancreatic manifestations seen in patients with von Hippel-Lindau(VHL) disease are subdivided into 2 categories:pancreatic neuroendocrine tumors(NET),and cystic lesions,including simple cyst and serous *** VHL-associated cystic lesions are generally asymptomatic and do not require any treatment,unless they are indistinguishable from other cystic tumor types with malignant *** pancreatic NET in VHL disease are non-functioning and have malignant potential,it is of clinical importance to find and diagnose these as early as *** will be recommended that comprehensive surveillance using dynamic computed tomography for abdominal manifestations,including pancreatic NET,should start from the age of 15 years in VHL *** sporadic non-functioning NET without VHL disease,in which surgical resection is generally recommended,VHL patients at lower metastatic risk of pancreatic NET should be spared the risks of operative resection.

关键词： Von Hippel-Lindau disease Pancreas Neuroendocrine tumor,Diagnosis Clinical protocols

Rescue of white egg 1 mutant by introduction of the wildtype Bombyx kynurenine 3-monooxygenase gene

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Insect Science 2007年第2期14卷 85-92页

作者： GUO-XING QUAN isao KOBAYASHI KATSURA KOJIMA KEIRO UCHINO TOSHIO KANDA HIDEKI SEZUTSU TORU SHIMADA TOSHIKI tamura Transgenic Silkworm Research Center National Institute of Agrobiological Sciences Tsukuba Ibaraki 305-8634 Japan Biomimetic Laboratory National Institute of Agrobiological Sciences Tsukuba Ibaraki 305-8634 Japan Department of Agricultural and Environmental Biology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan

In silkworms, the white egg 1 （w-1） mutant, which is characterized by white eyes and white eggs, is deficient in Bombyx kynurenine 3-monooxygenase （KMO） activity. To investigate whether the w-1 mutant phenotype is rescued by introducing the wild-type KMO gene, we constructed transgenic silkworms with the wild-type Bombyx KMO gene under the control of either the cytoplasmic actin gene promoter （A3KMO） or the native KMO gene promoter （KKMO）. We created two transgenic lines with A3KMO and one line with KKMO constructs. The eyes of adults in these lines were brown, and the eggs laid by the transgenic females were also brown. Reverse transcription-polymerase chain reaction（RT-PCR） analysis showed that the A3KMO silkworm lines expressed the transcript in the mid-gut, fat bodies, and Malpighian tubules. The KKMO line expressed the transcript only in the fat bodies and Malpighian tubules. The intensity of eye and egg color in the transgenic lines was proportional to the KMO expression level. Interestingly, transgenic larvae with the A3KMO construct had a light brown larval cuticle, but the KKMO line did not. These results indicate that the wild-type KMO gene can be used as a marker gene for visually screening transgenic silkworms.

关键词： Bombyx mori KMO marker gene transgenic w-1

Increasing spatial coverage in rough terrain and vegetated areas using InSAR optimized pixel selection:application to Tohoku,Japan

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Geo-Spatial Information Science 2022年第2期25卷 295-311页

作者： Tamer ElGharbawi Masayuki tamura Civil and Earth Resources Engineering Department Kyoto UniversityKyotoJapan

One of the major limitations of using Interferometric Synthetic Aperture Radar(InSAR)in time series analysis is the low-phase coherence associated with rough terrain and vegetated areas,which results in limited spatial coverage in such *** scatterers technique was introduced to overcome this limitation using time-series ***,identifying major scatterers within a pixel requires the single-looked pixels oversampling which can be a demanding process especially with large interferometric stacks and vast study ***,using multilooked temporal coherent pixels was proposed to increase processing efficiency and coverage by utilizing distributed targets,but this technique may exclude pixels with reliable phase returns because of their temporal varying neighboring *** this paper,we propose a technique to identify multilooked temporal stable pixels with reliable phase returns independent of their neighboring *** conduct a simulation analysis to relate the spatial coherence of a pixel with its expected temporal correlation in the time series analysis *** found that a liberal temporal correlation threshold of 0.53 in multilooked pixels stack is equivalent to a spatial coherence threshold of 0.2 when using number of looks of 9,which is considered acceptable in temporal coherent pixels,in terms of phase standard *** these findings to study the 2011 Tohoku earthquake in the northeastern part of Japan resulted in increasing the number of usable pixels and spatial coverage index by nearly 50.4%and 36.8%,respectively,compared to the temporal coherent ***,we propose an approach to integrate GPS observations with InSAR time series analysis,which resulted in deformation maps of the megathrust 2011 Tohoku earthquake with mean RMSE of 11.4 mm and a correlation of 98%in comparison to GPS observations.

关键词： 2011 Tohoku earthquake temporal stable pixels coherence phase noise GPS coverage index

Alterations of tumor suppressor and tumor-related genes in the development and progression of gastric cancer

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World Journal of Gastroenterology 2006年第2期12卷 192-198页

作者： Gen tamura Department of Pathology Yamagata University School of MedicineYamagata 990-9585Japan

The development and progression of gastric cancer involves a number of genetic and epigenetic alterations of tumor suppressor and tumor-related genes. The majority of differentiated carcinomas arise from intestinal metaplastic mucosa and exhibit structurally altered tumor suppressor genes, typified by p53, which is inactivated via the classic two-hit mechanism, i.e. loss of heterozygosity （LOH） and mutation of the remaining allele. LOH at certain chromosomal loci accumulates during tumor progression. Approximately 20% of differentiated carcinomas show evidence of mutator pathway tumorigenesis due to hMLH1 inactivation via hypermethylation of promoter CpG islands, and exhibit high-frequency microsatellite instability. In contrast, undifferentiated carcinomas rarely exhibit structurally altered tumor suppressor genes. For instance, while methylation of E-cadherin is often observed in undifferentiated carcinomas, mutation of this gene is generally associated with the progression from differentiated to undifferentiated carcinomas. Hypermethylation of tumor suppressor and tumor-related genes, including APC, CHFR, DAP- kinase, DCC, E-cadherin, GSTP1, hMLH1, p16, PTEN, RASSF1A, RUNX3, and TSLC1, can be detected in both differentiated and undifferentiated carcinomas at varying frequencies. However, the significance of the hypermethylation varies according to the analyzed genomic region, and hypermethylation of these genes can also be present in non-neoplastic gastric epithelia. Promoter demethylation of specific genes, such as MAGE and synudein y, can occur during the progressive stages of both histological types, and is associated with patient prognosis. Thus, while the molecular pathways of gastric carcinogenesis are dependent on histological background, specific genetic alterations can still be used for risk assessment, diagnosis, and prognosis.

关键词： Gastric cancer p53 E-cadherin hMLH1 Methylation

Pediatric kidney transplantation during the COVID-19 pandemic

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World Journal of Clinical Cases 2022年第34期10卷 12494-12499页

作者： Hiroshi tamura Department of Pediatrics Kumamoto UniversityKumamoto 860-8556Japan

A waiting list for non-emergency transplant medical care was recommended in the first half of 2020 due to the coronavirus disease 2019(COVID-19)***,the number of kidney transplants temporarily ***,the waiting list for transplant medical care was lifted in the latter half of 2020 with the establishment of a polymerase chain reaction test system and the spread of infection *** basic stance is to recommend vaccination to posttransplant recipients,recipients,and donors who are scheduled to undergo transplantation,and their families,with the start of vaccine therapy in *** mortality rate of patients undergoing kidney transplants who had COVID-19 is slightly higher than healthy persons,and acute kidney injury was reported to lead to graft ***,pediatric cases of severe disease are rare and without *** transplantation medical care will be continuously provided by implementing infection prevention and treatments based on the latest evidence,promoting donated kidney transplantation,and hoping that pediatric patients with renal failure will grow up healthy,both physically and mentally,and become independent members of society,just like healthy children.

关键词： Kidney transplantation COVID-19 pandemic Children

Response of understory vegetation over 10 years after thinning in an old-growth cedar and cypress plantation overgrazed by sika deer in eastern Japan

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Forest Ecosystems 2017年第2期4卷 93-102页

作者： Atsushi tamura Masanobu Yamane Division of Forest Research and Nature Restoration Planning Kanagawa Prefecture Natural Environment Conservation Center 657 Nanasawa AtsugiKanagawa 243-072 Japan

Background： Forest management strategies such as thinning have long been used to enhance ecosystem functions, especially in *** in plantations with high deer density, however, may not yield a desired increase in understory vegetation because deer graze on germinating plants after ***, we examine the changes in understory vegetation after thinning in plantations that have been overgrazed by sika deer to provide insight into the effects of thinning on ecosystem functions such as soil conservation and biological ***： We conducted our survey in the Tanzawa Mountains of eastern *** surveyed the change in understory vegetation within and outside of three deer exclosures on a single slope with three levels of understory vegetation cover： sparse（1%, exclosure ＂US＂）, moderate（30%, exclosure ＂MM＂）, and dense（80%, exclosure ＂LD＂） over10 years after a 30% thinning of an old-growth cedar and cypress plantation which was overgrazed by sika ***： Understory vegetation cover, biomass and species richness increased within and outside the ＂US＂ and＂MM＂ exclosures after thinning, and biomass was greater within than outside the exclosures at 10 years after *** species dominated both ＂US＂ and ＂MM＂ exclosures before thinning, and trees and shrubs dominated within the exclosures over time after *** contrast, unpalatable, grazing-tolerant, perennial,and annual species increased outside the ＂US＂ and ＂MM＂ *** noticeable changes were observed within and outside the ＂LD＂ exclosure when compared with the ＂US＂ and ＂MM＂ ***： Our results suggest that thinning a stand by 30% based on volume resulted in an increase in understory vegetation cover mainly composed of both unpalatable and grazing-tolerant species in a plantation forest where understory vegetation is sparse or moderate and sika deer density is *** emphasize that establishing deer exclosures or controlling deer is essential to ma

关键词： Biomass Deer exclosure Grazing-tolerant species Understory cover Unpalatable species