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Animal Models and Experimental Medicine 2020年第3期3卷 264-272页

作者： Katerina Bakela Maria Georgia Dimitraki Evangelia Skoufa irene Athanassakis Laboratory of Immunology Department of BiologyUniversity of CreteHeraklionCreteGreece

Background:Soluble major histocompatibility complex class II(sMHCII)molecules have been described to maintain tolerance through the suppression of autoreactive T *** order to evaluate their ability to rescue autoimmune hepatitis(AIH)symptoms,the present work attempted to administer sMHCII molecules to an in vitro as well as in vivo concanavalin A(ConA)-induced AIH ***:The in vitro AIH model consisted of splenocyte stimulation with ConA in the presence or absence of serum-isolated sMHCII *** in vivo ConAmodified model with or without sMHCII treatment was *** cytokine profile in culture supernatants and serum was tested by *** markers were evaluated by immunofluorescence,while cell proliferation by tritiated thymidine *** symptoms were assessed by daily observations for the establishment of a disease severity scoring system and liver histology was evaluated using a biomolecular ***:The presence of sMHCII molecules in the ConA-stimulated cell cultures leads to a significant reduction of cell *** administration of sMHCII molecules to the ConA-treated animals showed a significant reduction in the levels of IL-2,IL-4,and IL-10,as well as a decrease in the number of spleen CD4+and CD8+*** development of a scoring system,it was shown that the sMHCII treatment was accompanied by a slower progression of the disease,while rescuing fibrotic liver ***:The results presented in this study confirm the ability of sMHCII proteins to alleviate autoimmune hepatitis,possibly highlighting new therapeutic approaches for autoimmune diseases.

关键词： autoimmune hepatitis cytokines immunosuppression inflammation

Data augmentation and machine learning techniques for control strategy development in bio-polymerization process

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Environmental Science and Ecotechnology 2022年第3期11卷 40-48页

作者： Sizhou Wei Zhiyuan Chen Senthil Kumar Arumugasamy irene Mei Leng Chew School of Computer Science University of NottinghamNottinghamNG81BBUnited Kingdom School of Computer Science University of Nottingham MalaysiaSemenyih43500Malaysia Department of Chemical and Environmental Engineering University of Nottingham MalaysiaSemenyih43500Malaysia School of Engineering Monash University MalaysiaSubang Jaya47500Malaysia

Machine learning has been increasingly used in ***,in organic chemistry and other experiment-based fields,data collected from real experiments are inadequate and the current coronavirus disease(COVID-19)pandemic has made the situation even *** limited data resources may result in the low performance of modeling and affect the proper development of a control *** paper proposes a feasible machine learning solution to the problem of small sample size in the biopolymerization *** avoid overfitting,the variational auto-encoder and generative adversarial network algorithms are used for data *** random forest and artificial neural network algorithms are implemented in the modeling *** results prove that data augmentation techniques effectively improve the performance of the regression *** machine learning models were compared and the experimental results show that the random forest model with data augmentation by the generative adversarial network technique achieved the best performance in predicting the molecular weight on the training set(with an R^(2) of 0.94)and on the test set(with an R^(2) of 0.74),and the coefficient of determination of this model was 0.74.

关键词： Bio-polymerization Variational autoencoder generative adversarial network Random forest Artificial neural network

The composition and diversity of natural regeneration of tree species in gaps under different intensities of forest disturbance

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Journal of Forestry Research 2021年第5期32卷 1843-1853页

作者： Maame Esi Hammond Radek Pokorný Daniel Okae-Anti Augustine Gyedu irene Otwuwa Obeng Department of Silviculture Faculty of Forestry and Wood TechnologyMendel University in BrnoZemedelska 361300 BrnoCzech Republic Department of Soil Science School of AgricultureUniversity of Cape CoastP.M.BCape CoastGhana Forestry Services Division(FSD) P.M.BKumasiGhana 4 Madina-AccraGhana

The positive ecological interaction between gap formation and natural regeneration has been examined but little research has been carried out on the effects of gaps on natural regeneration in forests under different intensities of *** study evaluates the composition,diversity,regeneration density and abundance of natural regeneration of tree species in gaps in undisturbed,intermittently disturbed,and disturbed forest *** Tano Forest Reserve in Ghana was the study area and three gaps each were selected in the three forest site *** circular subsampling areas of 1 m2 were delineated at 2 m spacing along north,south,east,and west transects within individual *** on natural regeneration<350 cm height were *** results show that the intensity of disturbance was disproportional to gap *** diversity differed significantly between undisturbed and disturbed sites and,also between intermittently disturbed and disturbed sites for Simpson’s(1-D),Equitability(J),and Berger-Parker(B-P)***,there was no significant difference among forest sites for Shannon diversity(H)and Margalef richness(MI)*** species composition on the sites *** density on the disturbed site was significantly higher than on the two other *** abundance and density of shade-dependent species on all sites identified them as opportunistic replacements of gap-dependent *** species giving way to shade tolerant species is a natural process,thus make them worst variant in gap regeneration.

关键词： Disturbed Intermittently disturbed Undisturbed Forest sites Gap regeneration

Association of autoimmune type atrophic corpus gastritis with Helicobacter pylori infection

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World Journal of Gastroenterology 2010年第1期16卷 83-88页

作者： Lea irene Veijola Aino Mirjam Oksanen Pentti Ilmari Sipponen Hilpi Iris Kaarina Rautelin Herttoniemi Hospital City of Helsinki Department of Bacteriology and Immunology Haartman InstituteUniversity of Helsinki Repolar Oy Box 2602101 EspooFinland HUSLAB Helsinki University Central Hospital Laboratory Department of Medical Sciences University of Uppsala

AIM:To study the association between Helicobacter pylori(***)infection and autoimmune type atrophic gastritis. METHODS:Twenty-three patients with different grades of atrophic gastritis were analysed using enzyme immunoassay-based serology,immunoblot-based serology,and histology to reveal a past or a present *** *** addition,serum markers for gastric atrophy(pepsinogenⅠ,pepsinogenⅠ/Ⅱand gastrin)and autoimmunity[parietal cell antibodies(PCA), and intrinsic factor(IF),antibodies]were determined. RESULTS:Of the 14 patients with severe gastricatrophy,as demonstrated by histology and serum markers,and no evidence for an ongoing *** infection,eight showed *** antibodies by *** eight had elevated PCA and 4/8 also had IF *** the six immunoblot-negative patients with severe corpus atrophy,PCA and IF antibodies were detected in *** the patients with low to moderate grade atrophic gastritis(all except one with an ongoing *** infection),serum markers for gastric atrophy and autoimmunity were seldom ***,one *** negative patient with mild atrophic gastritis had PCA and IF antibodies suggestive of a pre-atrophic autoimmune gastritis. CONCLUSION:Signs of *** infection in autoimmune gastritis,and positive autoimmune serum markers in *** gastritis suggest an etiological role for *** in autoimmune gastritis.

关键词： Helicobacter pylori Autoimmune gastritis Gastric atrophy Vitamin B12 deficiency

Enhancement of biogas potential of primary sludge by co-digestion with cow manure and brewery sludge

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International Journal of Agricultural and Biological Engineering 2015年第4期8卷 86-94页

作者： irene Nansubuga Noble Banadda Mohammed Babu Jo De Vrieze Willy Verstraete Korneel Rabaey Laboratory of Microbial Ecology and Technology(LabMET) Ghent UniversityCoupure links 653B-9000 GentBelgium Department of Agricultural and Bio-Systems Engineering Makerere UniversityP.O.Box 7062KampalaUganda National Water and Sewerage corporation Plot 39Jinja RoadP.O.Box 7053KampalaUganda

Anaerobic digestion(AD)has long been used to treat different types of organic wastes especially in the developed ***,organic wastes are still more often considered as a waste instead of a resource in the developing world,which contributes to environmental pollution arising from their *** study has been conducted at Bugolobi Sewage Treatment Plant(BSTP),where two organic wastes,cow manure and brewery sludge were co-digested with primary sludge in different *** study was done in lab-scale reactors at mesophilic temperature and sludge retention time of 20 *** main objective was to evaluate the biodegradability of primary sludge generated at BSTP,Kampala,Uganda and enhance its ability of biogas *** the brewery sludge was added to primary STP sludge at all proportions,the biogas production rate increased by a factor of *** was significantly(p<0.001)higher than observed gas yield(337±18)mL/(L·d))in the control treatment containing(only STP sludge).Co-digesting STP sludge with cow manure did not show different results compared to the control *** conclusion,Bugolobi STP sludge is poorly anaerobically degradable with low biogas production but co-digestion with brewery sludge enhanced the biogas production rate,while co-digestion with cow manure was not beneficial.

关键词： wastewater treatment co-digestion cow manure brewery primary sludge biogas energy recovery

Predicting sandy soil moisture content with hyperspectral imaging

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International Journal of Agricultural and Biological Engineering 2017年第6期10卷 175-183页

作者： Qi Haijun Jin Xiu Zhao Liu DEDO irene Maxime Li Shaowen School of Information and Computer Anhui Agricultural UniversityHefei 230036China University of Abomey-Calavi CotonouBenin

In this study,a rapid and non-invasive technology for predicting soil moisture content(SMC)was presented based on hyperspectral imaging(HSI).Firstly,a set of HSI system was developed to collect both spectral(400-1000 nm)and spatial(1620×841 pixels)information from sandy soil samples with variable SMC levels in the *** component analysis(PCA)transformation,K-means clustering,and several other image processing methods were performed to obtain a region of interest(ROI)of soil sample from the original HSI ***,256 optimal spectral wavelengths were selected from the average reflectance of the ROI,and 28 textural features were extracted using a gray-level co-occurrence matrix(GLCM).Data dimensionality reduction was conducted on both the spectral information and textural information by using a partial least square *** latent variables(LVs)extracted from the spectral information,four LVs extracted from the textural information and fused data were used to build regression models with a three-layer BPNN,*** results showed that all of the three calibration models achieved high prediction accuracy,particularly when using spectral information with R^(2)_(C)=0.9532 and RMSEC=***,validation models demonstrate that predicting SMC using fused data is more effective than using spectral reflectance and textural features separately,with a R^(2)_(P)=0.9350 and RMSEP=0.0141,thus proving that the HSI technique is capable of detecting SMC.

关键词： hyperspectral imaging soil moisture content image processing prediction model fused data BPNN regression

Impact of intraocular pressure fluctuations on progression of normal tension glaucoma

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International Journal of Ophthalmology(English edition) 2021年第10期14卷 1553-1559页

作者： Susanne Hopf Doris Schwantuschke irene Schmidtmann Norbert Pfeiffer Esther Maria Hoffmann Department of Ophthalmology University Medical Center MainzMainz 55131Germany Pediatric Dentistry Nieder-Olm 55268Germany Institute of Medical Biostatistics Epidemiology and Informatics(IMBEI)University Medical Center MainzMainz 55131Germany

AIM:To investigate short-and long-term intraocular pressure(IOP)fluctuations and fur ther ocular and demographic parameters as predictors for normal tension glaucoma(NTG)***:This retrospective,longitudinal cohort study included 137 eyes of 75 patients with NTG,defined by glaucomatous optic disc or visual field defect with normal IOP(<21 mm Hg),independently from therapy *** fluctuation,mean,and maximum were inspected with a mean follow-up of 38 mo[standard deviation(SD)18 mo].Inclusion criteria were the performance of minimum two 48-hour profiles including perimetry,Heidelberg retina tomograph(HRT)imaging,and optic disc *** impact of IOP parameters,myopia,sex,cup-to-disc-ratio,and visual field results on progression of NTG were analyzed using Cox regression models.A sub-group analysis with results from optical coherence tomography(OCT)was ***:IOP fluctuations,average,and maximum were not risk factors for progression in NTG patients,although maximum IOP at the initial IOP profile was higher in eyes with progression than in eyes without progression(P=0.054).The 46/137(33.5%)eyes progressed over the followup *** progression(at least three progression confirmations)occurred in 28/137 eyes(20.4%).Most progressions were detected by perimetry(36/46).Longterm IOP mean over all pressure profiles was 12.8 mm Hg(SD 1.3 mm Hg);IOP fluctuation was 1.4 mm Hg(SD 0.8 mm Hg).The progression-free five-year rate was 58.2%(SD 6.5%).CONCLUSION:Short-and long-term IOP fluctuations do not result in progression of *** functional changes are most likely to happen,NTG should be monitored with visual field testing more often than with other devices.

关键词： intraocular pressure intraocular pressure fluctuation glaucoma progression visual field optical optical coherence tomography

Microtubule Depolymerization Affects Endocytosis and Exocytosis in the Tip and Influences Endosome Movement in Tobacco Pollen Tubes

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Molecular Plant 2013年第4期6卷 1109-1130页

作者： Aurora irene Idilli Piero Morandini Elisabetta Onelli Simona Rodighiero Marco Caccianiga Alessandra Moscatelli Department of Biosciences Milan University Via Celoria 26 20133 Milan Italy Fondazione Filarete Milan University Viale Ortles 22 Milan Italy

Polarized organization of the cytoplasm of growing pollen tubes is maintained by coordinated function of actin filaments （AFs） and microtubules （MTs）. AFs convey post-Golgi secretory vesicles to the tip where some fuse with specific domains of the plasma membrane （PM）. Secretory activity is balanced by PM retrieval that maintains cell mem- brane economy and regulates the polarized composition of the PM, by dividing lipids/proteins between the shank and the tip. Although AFs play a key role in PM internalization in the shank, the role of MTs in exo-endocytosis needs to be characterized. The present results show that integrity of the MT cytoskeleton is necessary to control exo-endocytosis events in the tip. MT polymerization plays a role in promoting PM invagination in the apex of tobacco pollen tubes since nocodazole affected PM internalization in the tip and subsequent migration of endocytic vesicles from the apex for degradation. MT depolymerization in the apex and shank was associated with misallocation of a significantly greater amount of internalized PM to the Golgi apparatus and its early recycling to the secretory pathway. Fluorescence Recovery After Photobleaching （FRAP） experiments also showed that MT depolymerization in the tip region influenced the rate of exocytosis in the central domain of the apical PM.

关键词： pollen tube microtubules endocytosis exocytosis.

Immunohistochemical localization of glutathione S-transferase-pi in human colorectal polyps

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World Journal of Gastroenterology 2008年第26期14卷 4179-4184页

作者： Eleni Gaitanarou Eleni Seretis Dimitrios Xinopoulos Emmanuel Paraskevas Niki Arnoyiannaki irene Voloudakis-Baltatzis Department of Electron Microscopy-Cell Biology "G. Papanicolaou" Research Center of Oncology and Experimental Surgery"Agios Savvas" Anticancer Hospital of Athens Athens 11522Greece Department of Electron Microscopy-Cell Biology "G. Papanicolaou" Research Center of Oncology and Experimental Surgery"Agios Savvas" Anticancer Hospital of Athens Athens 11522Greece Department of Gastroenterology "Agios Savvas" Anticancer Hospital of AthensAthens 11522Greece Department of Gastroenterology "Agios Savvas" Anticancer Hospital of AthensAthens 11522Greece Department of Pathology of the Regional Anticancer-Oncologic Hospital of Athens "Agios Savvas" Athens 11522Greece Department of Electron Microscopy-Cell Biology "G. Papanicolaou" Research Center of Oncology and Experimental Surgery"Agios Savvas" Anticancer Hospital of Athens Athens 11522Greece

AIM:To investigate the distribution of the placental form of glutathione-S-transferase (GST) in colon polyps in order to evaluate the role of GST-pi in these tissues. METHODS: Sixteen polyp tissues removed at colonoscopy were examined. Tissues were investigated histologically and ultrastructurally. GST-pi expression was also analysed immunohistochemically, using peroxidase anti-peroxidase (PAP) method and immunogold labelling method, for light and electron microscope respectively. RESULTS: All polyp tissues examined were adenoma of low, mild and high-grade dysplasia as shown in the histopathological reports. Nevertheless, the examination of the above specimens with electron microscope revealed that 3 of 9 adenoma of mild dysplasia had ultrastuctural features similar to high-grade dysplasia adenoma. GST-pi was variably expressed in adenoma, with the lowest relative levels occurring in low-gradeadenoma and the highest levels found in high-grade adenoma. GST-pi was located mainly in undifferentiated epithelial cells. GST-pi positive particles were found in the cytoplasm and especially in the nucleus adjacent to the nuclear membrane of these cells. CONCLUSION:The overexpression of GST-pi in mildgrade adenomas with significant subcellular changes and in the majority of high-grade dysplasia adenoma suggests that this might be related to the carcinogenetic proceeding. Immunohistochemical localization of GST-pi in combination with ultrastructural changes indicate that GST-pi might be a sensitive agent for the detection of preneoplastic transformations in adenoma.

关键词： Glutathione S-transferase-pi Colorectal polyps Adenoma Electron microscopy Peroxidase anti-peroxidase method Immunogold method

On Modeling Bio-Scaffolds:Structural and Fluid Transport Characterization Based on 3-D Imaging Data

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Tsinghua Science and Technology 2009年第S1期14卷 20-23页

作者： NOTARBERARDINO Bruno BAKER Matthew TABOR Gavin TURNER irene YANG Lincoln School of Engineering Computing and Mathematics University of Exeter Exeter EX4 4QF United Kingdom Department of Mechanical Engineering University of Bath Bath BA2 7AY United Kingdom Shanghai Gaitech Scientific Instruments Co. Ltd.

Bio-scaffolds which are most commonly open celled porous structures are increasingly used for tissue engineering and regenerative medicine. A number of studies have shown that the bulk properties of such irregular structures are poorly modeled using idealized unit cell approaches. The paper therefore uses novel image based meshing techniques to explore both fluid flow and bulk structural properties of a bone scaffold, as accurate modeling of bio-scaffolds with non-uniform cellular structures is very important for the development of optimal scaffolds for tissue engineering application. In this study, a porous hydroxyapatite/tricalcium phosphate (HA/TCP) bone scaffold has been scanned in a Micro-CT scanner, and converted into a volumetric mesh using image processing software developed by the authors. The resulting mesh was then exported to commercial FEA and CFD solvers for analysis. Initial FEA and CFD studies have shown promising results and have highlighted the importance of accurate modeling to understand how microstructures influence the mechanical property of the scaffold, and to analyze flow regimes through the sample. The work highlights the potential use of image based meshing for the ad hoc characterization of scaffolds as well as for assisting in the design of scaffolds with tailored strength, stiffness, and transport properties.

关键词： bio-scaffolds material characterization finite element computational fluid dynamics dynamics microstructures