检索结果-南通市图书馆

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Cheng-Tan LI,Wei-Ping ZHANG,Yue-Mei YUAN,San-Hua FANG,Er-Qing WEI Department of Pharmacology,institute of Neuroscience,school of medicine,zhejiang university,hangzhou 310058, china

＜正＞5-Lipoxygenase（5-LOX） is the key enzyme metabolizing arachidonic acid to produce pro-inflammatory *** initial step of 5-LOX activation is its translocation to the nuclear *** cultured rat cortical neurons and PC 12 cells,we have found that 5-LOX is activated by oxygen-glucose deprivation（OGD） via NMDA receptor *** whether reactive oxygen species（ROS） is also involved in OGD-induced 5-LOX activation and whether 5-LOX inhibitors affect 5-LOX translocation in different patterns remain *** answer theses questions, in the present study,we permanently transfected human GFP-5-LOX into PC12 cells,and further determined the involvement of 5-LOX in OGD- and exogenous H202-induced injuries using various *** found new properties of the changes in 5-LOX activation and inhibition.（1） Using immunohistochemistry and GFP fluorescent imaging,we found that the transfected 5-LOX was localized in the nucleus,while the native 5-LOX in both the cytosol and nucleus.（2） After 2-h OGD and 2-h recovery,both cytosolic and nuclear 5-LOX were translocated to the nuclear envelope,which related to the increased the production of cysteinyl leukotrienes and the cell *** the agents used to inhibit 5-LOX,baicalein （0.01-10 mmmol/L）,baicalin（0.1-10 mmmol/L）,caffeic acid（0.1-10 mmol/L） and NDGA（0.01-1 mmmol/L） inhibited the translocation of the transfected 5-LOX,while MK886 and zileuton did *** the agents protected PC12 cells from the injury after 2-h OGD and 24-h recovery,and decreased the production of cysteinyl leukotrienes.（3） After 2-h OGD,ROS was increased within 1-h *** exogenous H202 also induced cell injury in time-dependent（12-24 h） and concentration -dependent（80-640 mmmol/L） manners,which was attenuated by the agents mentioned above.H202（160 mM） treatment for 40 min - 1.5 h induced translocation of the transfected 5-LOX to the nuclear envelope,which was also inhibited by baicalein,baicalin,caffeic acid and NDGA,but

关键词： 5-lipoxygenase transfection oxygen-glucose deprivation(OGD) PC12 cells reactive oxygen species H2O2

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

用噬菌体展示筛选Gal-α-1,3-Gal的模拟肽

引用

生物化学与生物物理进展 2003年第3期30卷 461-465页

作者：张晖詹金彪许林海严志焜王克夷浙江大学医学院生物化学教研室杭州310006 浙江省人民医院杭州310014 中国科学院上海生物化学与细胞生物学研究所上海200031

猪心移植是解决心脏移植供体少的有效方法 .由于猪心血管内皮细胞表面抗原半乳糖 α 1,3 半乳糖 (Gal α 1,3 Gal) ,能与人体内预存的天然抗体结合产生超急性排斥反应 (HAR) ,而无法应用于临床 .为了解决这一难题 ,应用噬菌体展示技... 详细信息

猪心移植是解决心脏移植供体少的有效方法 .由于猪心血管内皮细胞表面抗原半乳糖 α 1,3 半乳糖 (Gal α 1,3 Gal) ,能与人体内预存的天然抗体结合产生超急性排斥反应 (HAR) ,而无法应用于临床 .为了解决这一难题 ,应用噬菌体展示技术 ,筛选出一个能与抗B型血单克隆抗体 (mABanti B)特异性结合的阳性噬菌体克隆 ,应用酶联免疫吸附测定 (ELISA)和竞争性ELISA结果表明 ,所获得的阳性噬菌体克隆能特异性地与mABanti B结合 ,并且这种结合可被蜜二糖 (具有Gal α 1,6 Glc的结构 )所竞争抑制 .由此推测 ,筛选到的噬菌体阳性克隆很可能就结合在蜜二糖与mABanti B结合的位点 .同时 ,进行了阳性噬菌体克隆的抑制猪红细胞凝集活性试验 ,试验结果表明 ,此阳性克隆不仅可抑制Gal α 1,3 Gal与抗体的结合 ,也可抑制Gal α 1,3 Gal与西非单叶豆凝集素 (GS I B4)的结合 .此噬菌体阳性克隆测序后 ,得小肽序列为CCWLLRQPVRFVRSIRS .鉴于以上的结果 ,认为此小肽可以作为Gal α 1,3 Gal的模拟肽。

关键词：噬菌体筛选 Gal-α-1 3-Gal 模拟肽器官移植

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

血管生成素对细胞核内功能的体外分析体系

引用

生物化学与生物物理学报 2003年第4期35卷 350-354页

作者：李凌云张耀洲郑一凡胡国富许正平浙江大学医学院公共卫生系浙江大学生命科学学院生物化学研究所浙江大学医学院公共卫生系哈佛大学医学院生物化学与生物物理及医学研究中心浙江大学医学院公共卫生系杭州310031 浙江大学生命科学学院生物化学研究所杭州310029杭州310029杭州310031剑桥02139杭州310031

利用人血管内皮细胞的细胞核而建立的体外转录分析体系可灵敏地定量测定血管生成素促进的RNA转录。反应体系中血管内皮细胞核先在缓冲液中与血管生成素混合 ,然后加四种核糖核苷三磷酸混合物启动反应 ,并以 [α 3 2 P]CTP作为示踪剂确... 详细信息

利用人血管内皮细胞的细胞核而建立的体外转录分析体系可灵敏地定量测定血管生成素促进的RNA转录。反应体系中血管内皮细胞核先在缓冲液中与血管生成素混合 ,然后加四种核糖核苷三磷酸混合物启动反应 ,并以 [α 3 2 P]CTP作为示踪剂确定新生RNA的产量。结果显示 ,该体外分析体系的最适反应温度为 30℃ ,最佳反应时间是 30min ,在此反应条件下血管生成素可定量促进RNA转录 ,并存在着剂量效应。研究发现 ,体系中过高浓度的血管生成素降解RNA产物 ,提示细胞具有控制该因子在细胞核内积聚的生物学机制 ,以保证其在细胞内恰当地发挥作用。抑制血管生成素诱导的RNA转录可能可以抑制血管新生 ,因而可能是治疗肿瘤的一个新的分子靶。

关键词：血管新生血管生成素 RNA转录分子靶

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A slice preparation of rat pancreatic islets for patch-clamp recordings in single β cells

A slice preparation of rat pancreatic islets for patch-clamp...

引用

2008北京国际生理科学学术大会

作者： WU Zheng-Yong~(1,2),ZHENG Liang-Hong~1,DOU Hai-Qiang~1,ZHU Kuai-Chang~1,LIU Chang~1,YAO Li-Juan~1,YU Xiao~1,ZHANG Claire Xi~1,SHEN Ying~2,ZHOU Zhuan~1 1 institute of Molecular medicine,Peking university,Beijing 100871,china 2 Department of Neurobiology,zhejiang university school of medicine,hangzhou 310031,china

Pancreatic isletβcells are responsible for blood glucose control by releasing *** cells sense blood glucose by ATP-receptor,which controls Ca entry and insulin *** disorder inβcell may lead to the severe disease *** fighting diabetes,patch clamp recording in cultureβcells is a powerful tool to examine mechanisms of insulin ***,βcell is metabolic type;it is difficult to prepare primary culture of rat or mouseβcells for *** overcome problem inβcell culture,we aim to prepare slices of pancreas,which enables patch clamp *** gelling-agarose was injected into the distally clamped bile duct and cooled it with an ice-cold extracellular solution,which enhance the mechanical strength for ***βcells in slices are easily patch-clamped,and its voltage-gated currents and evoked secretion can be recorded under whole-cell configuration.

关键词： Pancreatic islet b cells patch-clamp voltage-gated currents ATP-receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

DIALYZABLELEUCOCYTEEXTRACTSMEDIATEDTRANSFEROFSPECIFICDELAYED-TYPEHYPERSENSITIVITYRESPONSETOTAEKYLEUSTRICLENTENTUSHEMOCYANIN

引用

潍坊医学院学报 1993年第4期 270-272页

作者： Li Zailian Feng Yongtang Miao Naifa Yang Bingzhi Department of institute of Immunoloy,Current adress:Johns Hopkins university school of medicine and Department of Immunology and infectious diseases,Baltimore,Maryland 21205,USA.

Dialyzable leucocyte extracts (DLE)containing transfer factor (TF)was prepared from a pig which was primed by Taekypleus Triclentus Hemocyanin(TTH) according to an effective immunization *** porcine DLE is capable oftransferring TTH-specific delayed-type hypersensitivity (DTH) response to human *** results implicated that cellular immunities in procine and human speciescan be croos-activated,that specific DLE can be used in the treatment of infectious dis-ease such as hepatitis B and epidemic hemorrhagic fever,and that pigs provide an alter-native for preparing DLE for extensive human use.

关键词： taekypleus triclententus hemocyanin delayed-type hypersensitivity dialyzable leucocyte extracts

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

一种新的肝细胞生成素(HPO)转录本及其生物学活性

引用

生物化学与生物物理学报 2002年第2期34卷 225-230页

作者：闾军许望翔詹轶群崔晓林蔡卫民贺福初杨晓明浙江大学医学院第一附属医院传染病研究所军事医学科学院放射医学研究所军事医学科学院放射医学研究所杭州310003 北京100850

利用 5′RACE技术从人胎肝组织中分离一种新形式的肝细胞生成素 (HPO 2 0 5 )cDNA ,其编码蛋白质氨基酸序列的N端较已报道的人肝细胞生成素HPO(hepatopoietin)多 80个氨基酸 ,推测其蛋白质分子量为 2 3kD。RT PCR检测HPOmRNA在多种肝癌... 详细信息

利用 5′RACE技术从人胎肝组织中分离一种新形式的肝细胞生成素 (HPO 2 0 5 )cDNA ,其编码蛋白质氨基酸序列的N端较已报道的人肝细胞生成素HPO(hepatopoietin)多 80个氨基酸 ,推测其蛋白质分子量为 2 3kD。RT PCR检测HPOmRNA在多种肝癌细胞中表达 ,Western印迹可检测到 2 3kDHPO 2 0 5表达 ,表明此种形式HPO在自然状态下存在。将构建的HPO 2 0 5真核表达载体转染入COS 7细胞 ,其表达蛋白质能够刺激HepG2肝癌细胞DNA合成 ;将HPO 2 0 5、HPO和荷空表达载体分别转染入低水平表达HPO的Bel 740 2肝癌细胞株 ,发现HPO 2 0 5比HPO具有较强的激活MAPK磷酸化的活性。细胞周期分析稳定转染HPO 2 0 5 ,HPO细胞的增殖周期也支持这一结论。这些结果表明HPO 2 0 5具有刺激肝源性细胞增殖的活性 ,并提示HPO 2 0 5可能较HPO有更强的生物学活性

关键词： HPO 细胞增殖 MAPK HPO-205

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Oncogenesis of RON receptor tyrosine kinase:a molecular target for malignant epithelial cancers

引用

Acta Pharmacologica Sinica 2006年第6期27卷 641-650页

作者： Ming-hai WANG~(2,3,4)Hang-ping YAO~2 Yong-qing ZHOU~2 2 Laboratory of Chang-Kung Scholars Program for Tumor Biology,First Affiliated Hospital,zhejiang university school of medicine,hangzhou 310003.china 3 Cancer Biology Center and Department of Pharmaceutical Sciences,Texas Tech university Health Sciences Center school of Pharmacy,Amarillo,TX 79106,USA 3Cancer Biology Center and Department of Pharmaceutical Sciences Texas Tech University Health Sciences Center School of Pharmacy Amarillo TX 79106 USA 2Laboratory of Chang-Kung Scholars Program for Tumor Biology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China 2Laboratory of Chang-Kung Scholars Program for Tumor Biology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

Recepteur d’origine nantais(RON)belongs to a subfamily of receptor tyrosinekinases(RTK)with unique expression patterns and biological *** isactivated by a serum-derived growth factor macrophage stimulating protein(MSP).The RON gene transcription is essential for embryonic development and critical inregulating certain physiological *** studies have indicated thataltered RON expression contributes significantly to cancer progression *** primary tumors,such as colon and breast cancers,overexpressionof RON exists in large numbers and is often accompanied by the generation ofdifferent splicing *** RON variants direct a unique program thatcontrols cell transformation,growth,migration,and invasion,indicating that al-tered RON expression has the ability to regulate motile/invasive *** activities were also seen in transgenic mice,in which targeted expression ofRON in lung epithelial cells resulted in numerous tumors with pathological fea-tures of human bronchioloalveolar ***,abnormal RON activation isa pathogenic factor that transduces oncogenic signals leading to uncontrolledcell growth and subsequent malignant *** these facts,RON and its variants can be considered as potential targets for *** using small interfering RNA and neutralizing mono-clonal antibodies demonstrated that suppressing RON expression and activationdecreases cancer cell proliferation,increases apoptotic death,prevents tumorformation in nude mice,and reduces malignant ***,blocking RONexpression and activation has clinical significance in reversing malignant pheno-types and controlling tumor growth.

关键词： growth factor receptor signal transduction tumorigenesis monoclonal antibody small molecule inhibitor therapeutics

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Effects of vasoactive intestinal peptide on phenotypic and functional maturation of dendritic cells

Effects of vasoactive intestinal peptide on phenotypic and f...

引用

第十七次全国中西医结合肝病学术会议

作者： Jie Lu~a Ming-Hua Zheng~a Jie Yan~b Yong-Ping Chen~(a,§), Jian-Ping Pan~(b,§) a Department of infectious and Liver diseases, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 323000, P R. china b Department of Microbiology and Parasitology, zhejiang university school of medicine, hangzhou 310058, P R. china

The present study was designed to investigate the effects of vasoactive intestinal peptide (VIP) on differentiation, maturation of dendritic cells (DCs) in vitro. DCs were derived from the murine bone marrow hemopoietic progenitor cells by culturing in RPMI 1640 complete medium supplemented with GM-CSF and IL-4 in the presence or absence of various concentrations of vasoactive intestinal peptide (VIP) and lipopolysaccharide (LPS) The phenotype of DCs was analyzed by flow cytometry. Mixed leukocyte reaction (MLR) was employed to measure the capacity of DC to stimulate the allogeneic T cells. IL-12p70 secretion by DC was examined by ELISA. In the absence of LPS, VIP, in a dose dependent manner, up-regulated the expression of CDS0, CD86, CD54 and CD40, but down-regulated the expression of MHC class Ⅱ molecule (Ia). In the presence of LPS, VIP also dose dependently up-regulated the expression of CD80, CD86, CD54 and CD40, and down-regulated the expression of Ia. The capacity to stimulate alloreactive T cells and the production of IL-12p70 by DC were significantly augmented by VIP when compared with VIP-untreated DCs. These data suggest that VIP could promote the phenotypic and functional maturation of DCs, hereby regulating the type and outcome of the conducting immune response.

关键词： Dendritic cells Mixed leukocyte reaction IL-12 Costimulatory molecules Vasoactive intestinal peptide

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Detect early stage lung cancer by a LAMP microfluidic chip system with a real-time fluorescent filter processor

引用

Science china Chemistry 2012年第4期55卷 508-514页

作者： WANG TongZhou1, ZHANG Ye1, HUANG GuoLiang1,2,3, WANG Can2, XIE Lan1, MA Li2, LI ZhiYong2, LUO XianBo2, TIAN Hao2, LI Qiang1, LI Xin1, LV ZhouYan1 & BAO XiaoFan11Department of Biomedical Engineering, school of medicine, Tsinghua university, Beijing 100084, china 2National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, china 3Yangtze Delta Region institute of Tsinghua university, hangzhou 330401, china 1. Department of Biomedical Engineering School of Medicine Tsinghua University Beijing 100084 China2. National Engineering Research Center for Beijing Biochip Technology Beijing 102206 China3. Yangtze Delta Region Institute of Tsinghua University Hangzhou 330401 China

This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.

关键词： microfluidic chip – isothermal amplification – lung cancer – confocal detection – non linear filter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of histamine H1 receptor antagonist on the epileptogenesis and the involved mechanism related to astrocytes

Effect of histamine H1 receptor antagonist on the epileptoge...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Wei-Wei HU~1 Ping HE~1 Hai-Jing YAN~1 Zheng-Hao XU~1 Qi FANG~1 Kai ZHONG~1 Zhong CHEN~(1 2*) 1 institute of Neuroscience Department of Pharmacology school of medicine zhejiang university hangzhou 310058 china 2 Department of Pharmacology College of Pharmaceutical Sciences zhejiang university hangzhou 310058 china

＜正＞Objective Histamine H1 receptor antagonists present epileptogenic activities such as inducing the seizure or promoting the progress of epilepsy during the ***,the effect of H1 receptor antagonists on the epileptogenesis after drug withdrawal is still unknown and the existed mechanism is *** our preliminary experiment,H1 receptor antagonist elevated the astrocyte extracelluar glutamate content,which is suggested to affect the neuron function and possibly involved in the *** the present study is to explore the effect of H1 receptor antagonists on the epileptogenesis after drug withdrawal and the astrocyte glutamate uptake related *** The afterdischarge threshold and behavioral stage of seizures during amygdaloid kindling acquisition was observed after 10- day drug withdrawal following two-week chronic diphenhydramine *** extracellular glutamate content,the expression,distribution and current of glutamate transporters,the expression of glutamine synthetase（GS）,and the morphology in astrocytes were investigated after administration of diphenhydramine and *** Chronic pretreatment of diphenhydramine reduced the afterdischarge threshold,accelerated the behavioral development of seizures, and decreased the number of stimulations required to seizure stage 2,3 or 4 during amygdaloid kindling acquisition after 10-day drug *** diphenhydramine and pyrilamine elevated the extracellular glutamate content,decreased the glutamate transporters GLT-1,GLAST and GS expression,but not affected the distribution of GLT-1 and *** withdrawal of drug did not recover the glutamate content and the protein expression during early *** glutamate transporter-mediated current was also decreased after diphenhydramine *** diphenhydramine and pyrilamine induced obvious vacuolization in cytoplasm,but without noticeable cytotoxicity at the same *** diphenhydramine and pyrilamin

关键词： H1 receptor antagonist epilepsy glutamate astrocyte

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：