Effects of vasoactive intestinal peptide on phenotypic and functional maturation of dendritic cells

作     者：Jie Lu~a Ming-Hua Zheng~a Jie Yan~b Yong-Ping Chen~(a,§), Jian-Ping Pan~(b,§) a Department of Infectious and Liver Diseases, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 323000, P R. China b Department of Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou 310058, P R. China 

会议名称：《第十七次全国中西医结合肝病学术会议》

会议日期：2008年

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

关 键 词：Dendritic cells Mixed leukocyte reaction IL-12 Costimulatory molecules Vasoactive intestinal peptide 

摘      要：The present study was designed to investigate the effects of vasoactive intestinal peptide (VIP) on differentiation, maturation of dendritic cells (DCs) in vitro. DCs were derived from the murine bone marrow hemopoietic progenitor cells by culturing in RPMI 1640 complete medium supplemented with GM-CSF and IL-4 in the presence or absence of various concentrations of vasoactive intestinal peptide (VIP) and lipopolysaccharide (LPS) The phenotype of DCs was analyzed by flow cytometry. Mixed leukocyte reaction (MLR) was employed to measure the capacity of DC to stimulate the allogeneic T cells. IL-12p70 secretion by DC was examined by ELISA. In the absence of LPS, VIP, in a dose dependent manner, up-regulated the expression of CDS0, CD86, CD54 and CD40, but down-regulated the expression of MHC class Ⅱ molecule (Ia). In the presence of LPS, VIP also dose dependently up-regulated the expression of CD80, CD86, CD54 and CD40, and down-regulated the expression of Ia. The capacity to stimulate alloreactive T cells and the production of IL-12p70 by DC were significantly augmented by VIP when compared with VIP-untreated DCs. These data suggest that VIP could promote the phenotypic and functional maturation of DCs, hereby regulating the type and outcome of the conducting immune response.