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MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

science Bulletin 2012年第Z2期 3804-3810页

作者： LUO ZhongQin,JIN LongGuo & QIU LiJuan * The National Key Facility for crop Gene Resources and Genetic Improvement(NFCRI),institute of crop science,chinese academy of agricultural sciences,beijing 100081,China

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5’RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词： soybean miR1511 miR1511 * GmRPL4a

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Analysis of differential expression proteins of a dwarf soybean mutant by proteomlcs

Analysis of differential expression proteins of a dwarf soyb...

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第十三届全国植物基因组学大会

作者： Wei He,Lijuan Qiu~* The National Key Facility for crop Gene Resources and Genetic Improvement(NFCRI), institute of crop science,chinese academy of agricultural sciences,100081 beijing,China

Plant height is an important trait for crops and dwarf plant becomes one of important objectives of soybean ***808 is a dwarf mutant derived from Ethylmethylsulfonate(EMS) mutation of soybean cultivar *** this study d...

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Long-Term Effect of No-Tillage on Soil Organic Carbon Fractions in a Continuous Maize cropping System of Northeast China

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Pedosphere 2010年第3期20卷 285-292页

作者： HUANG Shan SUN Yan-Ni RUI Wen-Yi LIU Wu-Ren ZHANG Wei-Jian institute of Applied Ecology Nanjing Agricultural University Nanjing 210095 (China) Jilin academy of agricultural sciences Changchun 130124 (China) Centre for Agroecology & Farming Systems Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing100081 (China)

Increasing evidence has shown that conservation tillage is an effective agricultural practice to increase carbon （C） sequestration in soils. In order to understand the mechanisms underlying the responses of soil organic carbon （SOC） to tillage regimes, physical fractionation techniques were employed to evaluate the effect of long-term no-tillage （NT） on soil aggregation and SOC fractions. Results showed that NT increased the concentration of total SOC by 18.1% compared with conventional tillage （CT） under a long-term maize （Zea mays L.） cropping system in Northeast China. The proportion of soil large macroaggregates （〉 2 000 μm） was higher in NT than that in CT, while small macroaggregates （250-2 000μm） showed an opposite trend. Therefore, the total proportion of macroaggregates （〉 2 000 and 250-2 000μm） was not affected by tillage management. However, C concentrations of macroaggregates on a whole soil basis were higher under NT relative to CT, indicating that both the amount of aggregation and aggregate turnover affected C stabilization. Carbon concentrations of intra-aggregate particulate organic matter associated with microaggregates （iPOM-m） and microaggregates occluded within macroaggregates （iPOM-mM） in NT were 1.6 and 1.8 times greater than those in CT, respectively. Carbon proportions of iPOM-n and iPOM-mM in the total SOC increased from 5.4% and 6.3% in CT to 7.2% and 9.7% in NT, respectively. Furthermore, the difference in the microaggregate protected C （i. e., iPOM-m and iPOM-mM） between NT and CT could explain 45.4% of the difference in the whole SOC. The above results indicate that NT stimulates C accumulation within microaggregates which then are further acted upon in the soil to form macroaggregates. The shift of SOC within microaggregates is beneficial for long-term C sequestration in soil. We also corroborate that the microaggregate protected C is useful as a pool for assessing the impact of tillage management on SOC storage.

关键词： carbon sequestration conservation tillage particulate organic matter physical fractionation soil aggregates

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The Effect of Accelerated Aging on the Energy Supply of Maize Seed

The Effect of Accelerated Aging on the Energy Supply of Maiz...

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第二届国际整合植物生物学学术研讨会

作者： Ze-Lin Wang,Chao Song,Yue Zhao,Xia Xin and Xin-Xiong Lu~* institute of crop science,chinese academy of agricultural sciences,beijing 100081,China

It is necessary to mobilize storage compounds to provide energy for seed *** starchy seeds,such as maize seeds,the energy for germination is mainly provided by *** proteomic analyses showed that in maize seed,the expression of some proteins responsible for energy supply,such as 3-phosphoglycerate kinase,malate dehydrogenase and ATP synthase, were significantly down-regulated by accelerated *** was presumed that the energy supply system was closely related to seed *** further study the effect of accelerated aging on energy supply system at the level of the catalytic activity of the enzyme and gene expression,we detected the activity of key enzymes of glycolysis and phosphate pentose pathway by spectrophotography,such as hexokinase,glucose-6-phosphate dehydrogenase and pyruvate ***,the expression of their related genes was determined by RT-PCR and the level of ATP was estimated by bioluminescence,so as to explore the effect of accelerating aging on the pathway and the efficiency of energy *** the present study,maize(Zea mays ***. Jiyu 8) seeds,with the initial germination of 96%,were accelerated aged at 50℃,and seeds with germination percentages of 85%,73%,54%,22%,0%were used for further *** results showed that with declining germination,the activities of the three enzymes and gene expression displayed a downward trend,and ATP content decreased in non-imbibing seeds after *** the imbibition process,the activity of hexokinase gradually increased in non-aging seeds,but this trend was inhibited in aging *** the seeds with germination percentage higher than 54%, the activity of phosphate-6-glucose dehydrogenase slightly decreased until 12 h imbibition,and then increased to 1.5-1.8 times that of dry seeds after 24 h of imbibition,whereas no obvious changes in seeds with germination lower than 22%were *** activity of pyruvate kinase in non-aging dry seeds was 1.16 umol NADH mg protein min,but

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Development and Application of EST-derived Molecular Markers Specific to Chromosome Arms of Rye(Secale cereale L.)

Development and Application of EST-derived Molecular Markers...

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2009整合植物生物学研讨会

作者： Hongxing Xu~(1*),Dongdong Yin~(1*),Lihui Li~(2**),Qiuxia Wang~2,Xiuquan Li~2,Weihua Liu~2, Xinming Yang~2 and Diaoguo An~(1**) 1 Center for agricultural Resources Research,institute of Genetics and Developmental Biology,chinese academy of sciences,Shijiazhuang 050021,China 2 institute of crop science,chinese academy of agricultural sciences,beijing 100081,China.

Rye(Secale cereale L.)genome carries abundant advantageous genes for wheat genetic *** to cytogenetic characterization,chromosome specific markers are more convenient and quick methods to identify the transfer of rye chromosome to common wheat(Triticum aestivum L.).Recently,more and more expressed sequence tag(EST)

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Diversity of waxy Gene in chinese Rice Germplasm Resource

Diversity of waxy Gene in Chinese Rice Germplasm Resource

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Chen You-Tao,Qiao Wei-Hua,Yang Qin-Wen~* The National Key Facility for crop Gene Resources and Genetic Improvement institute of crop science,chinese academy of agricultural sciences,beijing 100081 *Author for correspondence

Waxy gene;Diversity;SNP;Granule-bound starch synthase;Domestication;Rice

关键词： Waxy gene Diversity SNP Granule-bound starch synthase Domestication Rice

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Genetic Diversity among Parents of Hybrid Rice Based on Cluster Analysis of Morphological Traits and Simple Sequence Repeat Markers

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Rice science 2006年第3期 155-160页

作者： WANG Sheng-jun 1, LU Zuo-mei 1, WAN Jian-min 1, 2 (1State Key Laboratory of crop Genetics & Germplasm Enhancement, Nanjing agricultural University, Nanjing 210095, China 2institute of crop science, chinese academy of agricultural sciences, beijing 100081, China)

The genetic diversity of 41 parental lines popularized in commercial hybrid rice production in China was studied by using cluster analysis of morphological traits and simple sequence repeat (SSR) markers. Forty-one entries were assigned into two clusters (i.e. early or medium-maturing cluster; medium or late-maturing cluster) and further assigned into six sub-clusters based on morphological trait cluster analysis. The early or medium-maturing cluster was composed of 15 maintainer lines, four early-maturing restorer lines and two thermo-sensitive genic male sterile lines, and the medium or late-maturing cluster included 16 restorer lines and 4 medium or late-maturing maintainer lines. Moreover, the SSR cluster analysis classified 41 entries into two clusters (i.e. maintainer line cluster and restorer line cluster) and seven sub-clusters. The maintainer line cluster consisted of all 19 maintainer lines, two thermo-sensitive genic male sterile lines, while the restorer line cluster was composed of all 20 restorer lines. The SSR analysis fitted better with the pedigree information. From the views on hybrid rice breeding, the results suggested that SSR analysis might be a better method to study the diversity of parental lines in indica hybrid rice.

关键词： parental lines hybrid rice morphological trait simple sequence repeats clustering analysis genetic diversity pedigree

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Mining the candidate genes related to SCN based on the pedigree analysis

Mining the candidate genes related to SCN based on the pedig...

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第十三届全国植物基因组学大会

作者： Shan-shan Zhang,Yin-hui Li,Li-juan Qiu~* The National Key Facility for crop Gene Resources and Genetic Improvement(NFCRI), institute of crop science,chinese academy of agricultural sciences,beijing 100081,China

Soybean cyst nematode(SCN) was one of the most destructive pests for soybean production which could easily cause significant agricultural *** discovering the new SCN related genes or markers,17 cultivars from the pedi...

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EFFECT OF (60)COγ RAYS ON MUTATION INDUCTION IN TISSUE-CULTURED CHRYSANTHEMUM

EFFECT OF (60)COγ RAYS ON MUTATION INDUCTION IN TISSUE-CULT...

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全国作物生物技术与诱变技术学术研讨会

作者： WANG Jing LIU Luxiang ZHAO Shirong GUO Huijun ZHAO Linshu CHEN Wenhua (Key Laboratory of agricultural Nuclear Technology & Space Breeding, Ministry of Agriculture/institute of crop science, chinese academy of agricultural sciences, beijing 100081)

The microshoots of tissue-cultured chrysanthemum cv. Qiuzhishan were irradiated by Co γrays with doses of 5,10,20 and 30Gy, respectively. After irradiation the microcuttings were transferred to root-inducing medium and subculture medium respectively to study the inductive effects of γrays on rooting and shoot growth and multiplication on media. The plant growth and flower characters were investigated after the regenerated plantlets were transplanted out of the aseptic protected environment. The results showed that 20Gy was the lethal dose of Co γrays to irradiate the microshoots of tissue-cultured chrysanthemum cv. Qiuzhishan, while 10Gy was the optimum dose to induce mutations. After bloom, 8.2% of the plants in the treatment of 10Gy were selected for their flower color mutations. In contrast with the bright yellow flowers of the control, different shades of red color were observed in those mutant flowers. All the mutant plants were heritable homogeneous mutants.

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Polymorphism of drought-response gene TaSAP2-A1 and its association with agronomic traits in wheat

Polymorphism of drought-response gene TaSAP2-A1 and its asso...

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第十三届全国植物基因组学大会

作者： Jianzhong Chang,Jianan Zhang,Xinguo Mao,Ang Li,Jizeng Jia,Ruilian Jing~* National Key Facility for crop Gene Resources and Genetic Improvement,Key Lab of crop Resources and Utilization,Ministry of Agriculture/institute of crop science,chinese academy of agricultural sciences,beijing 100081,China

TaSAP2,a member of stress association protein(SAP) genes from wheat,is involved in response to several abiotic stresses,including drought,salt and *** this study,TaSAP2-A1,a TaSAP2 on chromosome 7A,and its flanking sequences were *** analysis indicated the average nucleotide diversity(π) of the whole region was *** highest nucleotide diversity occurred in the

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