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Acta Oceanologica Sinica 2013年第2期32卷 59-65页

作者： LIU Chenlin huang xiaohang LIU Jianguo Marine Ecological Center The First Institute of OceanographyState Oceanic Administration Institute of Oceanology the Chinese Academy of Sciences

Due to morphological plasticity and paucity of diagnostic morphological characters, the taxonomy of Kap- paphycus gets more and more confused with the expanding of commercial cultivation. In this study, the phylogenetic relationship of 13 strains of introduced Kappaphycus species in China was defined using DNA molecular markers, such as 18S rDNA, rbcL and cox2-cox3 spacer region. The resolutions obtained by three different molecular markers were compared： both cox2--cox3 spacer region and rbcL sequences are eligible in inter- species identification of Kappaphycus, whereas cox2-cox3 spacer region is more variable than rbcL sequence. There is several basepairs＇ discrepancy among 18S rDNA sequences, while it is 100% identical among both cox2-cox3 spacer region and rbcL sequences of the ten strains of K. alvarezii. We suppose that 18S rDNA sequence can provide more information in biogeography study of Kappaphycus than other two DNA sequences.

关键词： 18S rDNA, cox2-cox3 spacer region, molecular marker, Kappaphycus rbcL

Key genes expression of reductive tricarboxylic acid cycle from deep-sea hydrothermal chemolithoautotrophic Caminibacter profundus in response to salinity, pH and O_2

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Acta Oceanologica Sinica 2013年第2期32卷 35-41页

作者： HE Peiqing LIU Yan YUE Wenjuan huang xiaohang Key Laboratory of Marine Bioactive Substance State Oceanic Administration The First Institute of Oceanography State Oceanic Administration

CO2 fixation pathway of Caminibacter profundus, a chemolithoautotrophic e-Proteobacteria from deep-sea hydrothermal vent, was determined and characterized by genetic and enzymatic analyses. Gene expression of key enzymes for CO2 fixation in response to salinity, pH and O2 in Medium 829 were also investigated. The results demonstrate that C. profundus contained aclB, porA and oorA, the genes encoding key enzymes of reductive tricarboxylic acid （rTCA） cycle. However, genes fragments of cbbL and cbbMencoding key enzyme of Calvin cycle were not recovered. Key enzymatic activities of ATP citrate lyase （ACL）, pyruvate： ferredoxin oxidoreductase （POR） and 2-oxoglutarate： ferredoxin oxidoreductase （OOR） were also present in C. profun- dus. The combination of genetic and enzymatic analyses confirm that C. profundus adopted rTCA cycle for carbon assimilation. The results of aclB and oorA relative expressions of C. profundus demonstrate that the ranges of environmental factors for high genes expression were sea salt 3.0%-5.0% （optimum 3.0%）, pH 5.0-6.5（optimum pH 6.5）, anaerobic to microaerobic conditions （optimum 1.0% 02）. Gene expression pat- terns under different conditions show similar patterns with bacterial growth, revealing that key rTCA cycle genes provided molecular basis for bacterial growth and propagation. Our results suggest that C. profun- dus could regulate key genes of rTCA cycle for carbon assimilation and energy metabolism in response to environmental fluctuations in hydrothermal vent.

关键词： Caminibacter profundus reductive tricarboxylic acid (rTCA) cycle ATP citrate lyase pyruvate:ferredoxin oxidoreductase 2-oxoglutarate: ferredoxin oxidoreductase hydrothermal vent

Screening and Characterization of the Alkaline Protease Isolated from PLI-1, a Strain of Brevibacillus sp. Collected from Indonesia's Hot Springs

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Journal of Ocean University of China 2012年第2期11卷 213-218页

作者： WANG Shuai LIN Xuezheng huang xiaohang ZHENG Li Dewi Seswita Zilda The Key Laboratory of Marine Bioactive Substances The First Institute of OceanographySOAQingdao 266061P R.China Research Center for Marine and Fishery Product Processing and Biotechnology JakartaIndonesia

A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia's hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1 was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 26.54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2+ was found to promote protease activity at both low and high concentrations, whereas Cu2+ and Zn2+ could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.27%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.25%, 52.37% ± 0.25%, 62.47% ± 2.96% and 50.99% ± 0.24%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1 was a thermophilic protease and had a high-temperature stability and a pH stability.

关键词： hot spring thermophilic bacterium thermophilic protease identification

Identification of SNARE proteins in fish—Tilapia Oreochromis niloticus

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Acta Oceanologica Sinica 2007年第1期26卷 159-163,164页

作者： huang xiaohang LAM Patrick P L LIN Xuezheng LIU Chenlin BIAN Ji GAISANO Herbert Key Laboratory of Marine Bioactive Substances First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Department of Physiology and Medicine University of Toronto Toronto M5S 1A8 Canada

SNARE proteins are a group of membrane-associated proteins involved in exocytosis, secretion and membrane trafficking events in eukaryotic cells. Research on SNARE protein biology has become a more attractive field in recent years, which is applied to marine biology specifically to the fish Tilapia （Oreochromis niloticus）. Plasma membrane fractions of different tissues of Tilapia, including brain, liver-pancreas, intestine, skin and muscle, were extracted, and immuno-decorated with isoform-specific antibodies to the SNARE families and associated proteins. The presence of Syntaxins - 1 A, 2 and 3, SNAP - 23 and SNAP - 25, VAMP - 2, Munc - 18 - 1 and Munc - 13 in the brain was identified, which were differentially distributed in the other organ tissues of the fish Tilapia. The distinct distribution of SNARE and associated proteins will serve as the basis for further investigation into their special secretory function in these tissues of the fish.

关键词： SNARE proteins exocytosis secretion fish

Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus)

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Acta Oceanologica Sinica 2010年第1期29卷 116-119页

作者： CHAI Yingmei huang xiaohang CONG Bailin LIU Chenlin LIU Shenghao College of Marine Life Sciences Shandong University at Weihai Weihai 264209 China Marine Bioactive Substances Laboratory the First Institute of Oceanography StateOceanic Administration Qingdao 266061 China

In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot （Scophthalmus maximus）. Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dma TEA or 5 mmol/dm^3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot.

关键词： patch-clamp technique potassium channel turbot peripheral blood lymphocyte

Phylogenetic analysis and in vitro culture of mosses fromthe Antarctic Fildes Peninsula

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Advances in Polar Science 2014年第2期25卷 97-104页

作者： LIU Shenghao ZHANG Zhaohui WANG Nengfei CONG Bailin ZHANG Pengying LIN Xuezheng huang xiaohang Marine Ecology Research Center The First Institute of Oceanography State Oceanic Administration College of Life Science Shandong University

Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species. In the present study, the small subunit ribosomal RNA （SSU rDNA） and internal transcribed spacers of the nuclear ribosomal DNA （ITS rDNA） were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica. Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses. In addition, in our phylogenetic analysis, one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species. Moreover, the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated. The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation.

关键词： phylogenetic tree tissue culture protonemata formation Pohlia nutans

Toxoplasma gondii infection in children after allogeneic hematopoietic stem cell transplantation: A case report and literature review

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Pediatric Investigation 2021年第3期5卷 239-243页

作者： Lijuan Luo Nan Shen Wenjuan Chen Chengjuan Luo xiaohang huang Yuelian Jiang Qing Cao Department of Infectious Diseases Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Hematology and Oncology Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Pharmacy Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

Introduction:Toxoplasmosis is a life-threatening complication after hematopoietic stem cell transplantation(HSCT).However,for several reasons,clinicians know little about Toxoplasma *** presentation:We report a case of toxoplasmosis that was diagnosed by bone marrow smear and metagenomic next-generation sequencing(mNGS)after HSCT in a ***,we summarize the characteristics of toxoplasmosis after pediatric HSCT reported in the literature published in ***:Clinicians should increase their awareness of toxoplasmosis in children after HSCT and implement pre-transplant screening and post-transplant monitoring and prevention in future according to the national conditions of our country.

关键词： Toxoplasmosis Hematopoietic stem cell transplantation Pediatric

Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean

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Acta Oceanologica Sinica 2003年第4期22卷 643-649页

作者： Lin Xuezheng1,Yang Xiuxia2,Bian Ji1,huang xiaohang1 1. First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China 2. Fishery College, Ocean University of China,Qingdao 266003,China First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Fishery College Ocean University of China Qingdao 266003 China

A strain of psychrophilic bacterium, 2-5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern Ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2+ and Zn2+.

关键词： Southern Ocean deep sea psychrophilic bacteria low-temperature lipase