检索结果-南通市图书馆

Cloning, expression and chitin-binding activity of two peritrophin-like protein genes in the common cutworm, Spodoptera fitura

在线全文

学校读者我要写书评

暂无评论

Insect Science 2014年第4期21卷 449-458页

作者： Wei-Jun Chen Li-Xia huang Dan Hu Li-Yan Liu Jun Gu Li-hua huang qi-Li Feng Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life Sciences South China Normal UniversityGuangzhou China

Insect midgut secretes a semi-permeable peritrophic membrane （PM）, which plays important roles in protecting the midgut and helping with food digestion. The lep- idopteran larvae produce type 1 PM, which is degraded when insects develop into the metamorphic stages. However, the underlying mechanism is unclear. In the present study, two peritrophin-like proteins （peritrophin-57 and 37） were identified from the midgut expression sequence tag library and transcriptome of the common cutworm, Spodoptera litura. The temporal and spatial expression patterns and responses to the induction of 20- hydroxyecdysone （20E） and starvation were examined by real-time quantitative polymerase chain reaction according to their common sequence region. The chitin-binding activity was also studied using a competitor, calcofluor. The open reading frames are 1 554 and 1 020 bp, respectively. They shared four highly conserved peritrophin-A domains and were ex- pressed only in the midgut rather than in the other tissues, including fat body, epidermis, Malpighian tube and hemolymph. Their transcriptional expression could only be detected at the larval stages rather than in eggs, prepupae, pupae and adults. The purified protein of peritrophin-37 bound to chitin in a dose-dependent manner. These results indicate that the two proteins are peritrophins, the structural components of PM. In addition, the messenger RNA levels of the two peritrophins were significantly down-regulated by 20E injection, whereas feeding/starvation had no effect on the expression. These findings suggest that the increase of20E titer may be an important factor which controls the degradation of PM during metamorphosis.

关键词： metamorphosis peritrophic membrane peritrophin starvation 20-hydroxyecdysone

Fluorescence emission from excited molecular ions in intense femtosecond laser fields

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of physics 2013年第1期8卷 34-38页

作者： Yun-Chen Wang Cheng-Yin Wu Yuan-Xing Liu Shao-hua Xu qi-huang Gong State Key Laboratory for Mesoscopic Physics Department of Physics Peking University Beijing 100871 China

Strong fluorescence emissions were observed for nitrogen, carbon monoxide, and carbon dioxide molecules in intense femtosecond laser fields. These emissions can be assigned to the transitions of the molecular ions from the excited electronic states to the ground electronic states. The formation mechanisms were discussed and the lifetimes were measured for these excited molecular ions in intense laser fields.

关键词： tunneling ionization molecular orbital fluorescence

Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan huang Yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li Hai-Wei Pu Xin-Yian Guo Jian-Gang Shen Su-hua qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

Meningeal lymphatic vessel crosstalk with central nervous system immune cells in aging and neurodegenerative diseases

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 763-778页

作者： Minghuang Gao Xinyue Wang Shijie Su Weicheng Feng Yaona Lai Kongli huang Dandan Cao qi Wang Science and Technology Innovation Center Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Institute of Clinical Pharmacology Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina The Sixth Affiliated Hospital Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain metabolites but also contribute to antigen delivery and immune cell activation. The advent of novel genomic technologies has enabled rapid progress in the characterization of myeloid and lymphoid cells and their interactions with meningeal lymphatic vessels within the central nervous system. In this review, we provide an overview of the multifaceted roles of meningeal lymphatic vessels within the context of the central nervous system immune network, highlighting recent discoveries on the immunological niche provided by meningeal lymphatic vessels. Furthermore, we delve into the mechanisms of crosstalk between meningeal lymphatic vessels and immune cells in the central nervous system under both homeostatic conditions and neurodegenerative diseases, discussing how these interactions shape the pathological outcomes. Regulation of meningeal lymphatic vessel function and structure can influence lymphatic drainage, cerebrospinal fluid-borne immune modulators, and immune cell populations in aging and neurodegenerative disorders, thereby playing a key role in shaping meningeal and brain parenchyma immunity.

关键词： central nervous system meningeal lymphatic vessels immunity myeloid cells lymphatic cells neurodegenerative disease

在线全文

学校读者我要写书评

暂无评论

钛合金磨削加工性的改善

西北工业大学学报 1989年第4期7卷 355-363页

作者：任敬心华定安黄奇张智龙西北工业大学

本文分析了磨削钛合金的砂轮磨损机理,提出了改善钛合金磨削加工性的措施。试验研究结果表明,若采用所推荐的砂轮及磨削油,则可使砂轮粘附、磨削力和磨削温度明显降低,磨削比显著提高,极大地改善了钛合金的磨削加工性。

关键词：钛合金磨削加工性陶瓷CBN砂轮极压磨削油

Amperometric Glucose Sensor Based on Batch CuO-Modified Screen-Printed Silver-Carbon Electrodes

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 35-41页

作者： XIA Haiyan huang Yin YANG hua WANG Xintian SHI Jianping LIU Lixia FU Yacheng QUAN Changyun LI Suyuan Cofoe Medical Technology Company Limited Changsha 410000China Institute of New Energy Hexi UniversityZhangye 734000China Chemistry and Chemical Engineering Central South UniversityChangsha 410000China

CuO nanoparticles were successfully synthesized via a two-jet electrospun method,and then screen-printed on silver-carbon electrodes,forming CuO-modified Ag-C(CuO/Ag-C)disposable strip *** natural environment condition for glucose detection,the obtained CuO/Ag-C electrodes show a high sensitivity of 540 nA·mM^(-1)·cm^(-2),and a low limit of detection(0.68 mM)in a wide linear response range of 0.68 mM and 3 mM(signal/noise=3),*** addition,the CuO/Ag-C electrodes also exhibit excellent anti-interference,air stability and *** a result,the fabrication of CuO nanoparticles via an electrospun process and the technique of screen-printed electrodes are of great significance for glucose detection.

关键词： electrochemical sensor CuO nanoparticles glucose detection screen-printing technique

Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： Yanting Liu Wei huang Jiayi Wen Xin Xiong Ting Xu qi Wang Xiusheng Chen Xianxian Zhao Shihua Li Xiaojiang Li Weili Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution

Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu Guo Lanyue Zhang Gening Du Hao You Junfeng huang Mudong Tu hua Li Yong Peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： X ray photoelectron spectroscopy

Advanced Functional Electromagnetic Shielding Materials:A Review Based on Micro‑Nano Structure Interface Control of Biomass Cell Walls

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第1期17卷 98-134页

作者： Yang Shi Mingjun Wu Shengbo Ge Jianzhang Li Anoud Saud Alshammari Jing Luo Mohammed A.Amin hua qiu Jinxuan Jiang Yazeed M.Asiri Runzhou huang hua Hou Zeinhom M.El‑Bahy Zhanhu Guo Chong Jia Kaimeng Xu Xiangmeng Chen Co‑Innovation Center of Efficient Processing and Utilization of Forest Resources College of Materials Science and EngineeringNanjing Forestry UniversityNanjing 210037People’s Republic of China State Key Laboratory of Efficient Production of Forest Resourced Beijing Forestry UniversityQinghua East Road 35Haidian DistrictBeijing 100083People’s Republic of China Department of Physics Faculty of Sciences‑ArarNorthern Border UniversityArar 91431Saudi Arabia Department of Chemistry College of ScienceTaif UniversityP.O.Box 1109921944 TaifSaudi Arabia Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China Integrated Composites Lab Department of Mechanical and Construction EngineeringNorthumbria UniversityNewcastle Upon Tyne NE18STUK College of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024People’s Republic of China Department of Chemistry Faculty of ScienceAl-Azhar UniversityNasr CityCairo 11884Egypt Yunnan Provincial Key Laboratory of Wood Adhesives and Glued Products International Joint Research Center for Biomass MaterialsSouthwest Forestry UniversityKunming 650224People’s Republic of China School of Science Henan Agricultural UniversityZhengzhou 450002People’s Republic of China

Research efforts on electromagnetic interference(EMI)shielding materials have begun to converge on green and sustainable biomass *** materials offer numerous advantages such as being lightweight,porous,and *** to their porous nature,interfacial compatibility,and electrical conductivity,biomass materials hold significant potential as EMI shielding *** concerted efforts on the EMI shielding of biomass materials have been reported,this research area is still relatively new compared to traditional EMI shielding *** particular,a more comprehensive study and summary of the factors influencing biomass EMI shielding materials including the pore structure adjustment,preparation process,and micro-control would be *** preparation methods and characteristics of wood,bamboo,cellulose and lignin in EMI shielding field are critically discussed in this paper,and similar biomass EMI materials are summarized and *** composite methods and fillers of various biomass materials were *** paper also highlights the mechanism of EMI shielding as well as existing prospects and challenges for development trends in this field.

关键词： Biomass materials Electromagnetic interference shielding Micro-nano structure interface control Conductivity