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Expression and alteration of insulin-like growth factor II-messenger RNA in hepatoma tissues and peripheral blood of patients with hepatocellular carcinoma

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World Journal of Gastroenterology 2005年第30期11卷 4655-4660页

作者： Zhi-Zhen Dong Deng-Fu Yao Deng-Bing Yao Xin-hua wu Wei wu Li-Wei Qiu Dao-Rong Jiang Jian-hua Zhu Xian-Yong Meng Department of Internal Medicine Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China Research Center of Clinical Molecular Biology Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China Institute of Neurosciences Nantong University Nantong 226001 Jiangsu Province China

AIM: To investigate the clinical values of serum free insulin-like growth factor Ⅱ (IGF-Ⅱ) levels and IGF-Ⅱ mRNA in hepatocellular carcinoma (HCC) tissues and peripheral blood for diagnosis of HCC and monitoring of extrahepatic ***: Total RNAs were extracted from HCC tissues or peripheral blood mononuclear cells from patients with HCC, liver diseases devoid of cancer, non-hepatic tumors,and healthy controls, respectively. IGF-Ⅱ cDNAs were synthesized through random primers and reversetranscriptase, amplified by polymerase chain reaction (PCR), and confirmed by DNA sequencing analysis. Serum free IGF-Ⅱ levels in patients with different liver diseases were analyzed by an enzyme-linked immunosorbent ***: The amplified fragments of IGF-Ⅱ mRNA by RT-PCR were identical to originally designed ones with a size of 170 bp and confirmed by sequencing *** dilution experiments revealed that the lowest sensitivity of our system was 2 ng/L of total RNA. The positive frequencies of IGF-Ⅱ mRNA were 100% in HCC tissues,53.3% in para-cancerous tissues, and 0% in non-cancerous tissues, respectively. The serum free IGF-Ⅱ levels were significantly higher in HCC than those in chronic hepatitis or liver cirrhosis. The positive frequency of circulating IGF-Ⅱ mRNA was 34.2% in HCC, no amplified fragment was found in other liver diseases, extrahepatic tumors,and normal controls, respectively. The circulating IGF-Ⅱ mRNA correlated with the stage of HCC, and its positive rate was 100% in HCC with extrahepatic metastasis and 35.5% in HCC with AFP-negative. No significant correlation was found between tumor sizes and circulating IGF-Ⅱ mRNA ***: The abnormal expressions of free IGF-Ⅱ and IGF-Ⅱ mRNA are useful tumor markers for HCC diagnosis, differentiation of extrahepatic metastasis and monitoring postoperative recurrence.

关键词： IGF-Ⅱ Hepatocellular carcinoma

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Smoothed Maximum Score Change-point Estimation in Binary Response Model

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Acta Mathematicae Applicatae Sinica 2006年第4期22卷 655-662页

作者： Min Yuan Lin-cheng Zhao Yao- hua wu Department of Statistics and Finance University of Science and Technology of China Hefei 230026 China

This paper studies the large sample properties of the change point estimates in binary response models. The estimate is obtained by maximizing the smoothed score function when the median of the latent error variable i... 详细信息

关键词： Change-point strong consistency VC class maximum score function

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Solidification Structure of Continuous Casting Large Round Billets under Mold Electromagnetic Stirring

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Journal of Iron and Steel Research International 2016年第4期23卷 329-337页

作者： Tao SUN Feng YUE hua-jie wu Chun GUO Ying LI Zhong-cun MA Metallurgical Engineering Research Institute University of Science and Technology Beijing Collaborative Innovation Center of Steel Technology University of Science and Technology Beijing Beiman Special Steel Co. Ltd.

The solidification structure of a continuous casting large round billet was analyzed by a cellular-automaton-finite-element coupling model using the ProCAST software. The actual and simulated solidification structures were compared under mold electromagnetic stirring （MEMS） conditions （current of 300 A and frequency of 3 Hz）. Thereafter, the solidification structures of the large round billet were investigated under different superheats, casting speeds, and secondary cooling intensities. Finally, the effect of the MEMS current on the solidification structures was obtained under fixed superheat, casting speed, secondary cooling intensity, and MEMS frequency. The model accurately simulated the actual solidification structures of any steel, regardless of its size and the parameters used in the continuous casting process. The ratio of the central equiaxed grain zone was found to increase with decreasing superheat, increasing casting speed, decreasing secondary cooling intensity, and increasing MEMS current. The grain size obviously decreased with decreasing superheat and increasing MEMS current but was less sensitive to the casting speed and secondary cooling intensity.

关键词： continuous casting large round billet solidification structure cellular-automaton-finite-element method mold electromagnetic stirring central equiaxed grain zone grain size

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Incubation and development of corrosion in microstructures of low alloy steels under a thin liquid film of NaCl aqueous solution

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International Journal of Minerals,Metallurgy and Materials 2010年第6期17卷 748-755页

作者： Wen-hua Zhang Shan-wu Yang Jia Guo Zhi-yong Liu Xin-lai He School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

Electrochemical measurement, optical microscopy, and scanning electron microscopy were employed to investigate the corrosion behavior of some low alloy steels. The steels were held under a thin liquid film of 0.5wt% NaCl aqueous solution. It is found that the steels with the same chemical composition but different micmstructures exhibit obviously different corrosion behaviors. However, the corrosion behavior of the steels with different compositions but the same microstructnres may be similar in the present investigation. The corrosion rate of bainite is slower than that of ferrite and pearlite. The corrosion products of bainite are uniform and fine. The size of carbon-rich phases produces a great impact on the corrosion of the steels, whether in the initial stage or in the long tenn. It is easy to induce large pitting for carbon-rich phases with large size, which damages the compactness of the rust layer.

关键词： alloy steel microstructure atmospheric corrosion liquid films scanning electron microscope

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General Formulae for Two Pseudo-differential Operator

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Communications in Theoretical Physics 2008年第6期49卷 1393-1396页

作者： ZHANG Da-Jun wu hua DENG Shu-Fang BI Jin-Bo Department of Mathematics Shanghai University Shanghai 200444 China Department of Mathematics East China University of Science and Technology Shanghai 200237 China Department of Mathematics Hangzhou Dianzi University Hangzhou 310018 China

Some general formulas in the Sato theory related to the nonisospectral KP and mKP hierarchies are derived for simplifying calculations.

关键词： Sato theory pseudo-differential operators general formulas nonisospectral KP and mKP hierar-chies

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Geochemical modeling of groundwater in southern plain area of Pengyang County,Ningxia,China

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Water Science and Engineering 2010年第3期3卷 282-291页

作者： Pei-yue LI Hui QIAN Jian-hua wu Jia DING School of Environmental Science and Engineering Chang＇an University Xi ＇an 710054 P. R. China

The purpose of this paper is to examine the evolution mechanisms of a hydrochemical field and to promote its benefits to the living standards of local people and to the local economy in the southern plain area of Pengyang County, in Ningxia, China. Based on understanding of the hydrogeological conditions in Pengyang County, the chemical evolution characteristics of groundwater in the plain area were analyzed. PHREEQC geochemical modeling software was used to perform hydrochemical modeling of water-rock interaction and to quantitatively analyze the evolution processes and the formation mechanisms of the local groundwater. Geochemical modeling was performed for two groundwater paths. The results showed that, along path 1, Na＋ adsorption played the leading role in the precipitation process and its amount was the largest, up to 6.08 mmol/L; cation exchange was significant along path 1, while along simulated path 2, albite accounted for the largest amount of dissolution, reaching 9.06 mmol/L, and the cation exchange was not significant. According to the modeling results, along the groundwater flow path, calcite and dolomite showed oversaturated status with a precipitation trend, while the fluorite and gypsum throughout the simulated path were not saturated and showed a dissolution trend. The total dissolved solids （TDS） increased and water quality worsened along the flow path. The dissolution reactions of albite, CO2, and halite, the exchange adsorption reaction of Na＋, and the precipitation of sodium montmorillonite and calcite were the primary hydrogeochemical reactions, resulting in changes of hydrochemical ingredients.

关键词： hydrogeochemistry water-rock reaction groundwater modeling Pengyang County

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Metabolic assessement of spleen infiltration by non-Hodgkin lymphoma using ^(18)F-FDG PET/CT:Preliminary clinical results

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Nuclear Science and Techniques 2009年第4期20卷 235-242页

作者： SUN Long PAN Weimin LUO Zuoming WEI Jihong ZHAO Long wu hua Minnan PET Center and Department of Nuclear Medicine No.1 Hospital of Xiamen Fujian Medical University Xiamen 316003 China

18F-FDG PET/CT was used for evaluation of spleen infiltration in patients with B-cell non-Hodgkin lymphoma(NHL).Five patients with histological diagnosed B-cell NHL underwent1 8F-FDG PET/CT *** integrated PET/CT image,spleen infiltration was considered when PET images revealed a discrete margin of solid splenic masses or diffuse lesion of spleen with higher maximum standardized uptake value(SUV) greater than those of normal liver *** images demonstrating a positive splenic index(>480 mL)or focal hypodensities were classified as positive for spleen *** the patients underwent systemic ***1 8F-FDG PET and CT results were compared with final *** patients had spleen infiltration originating from B-cell NHL at final *** diagnoses,which were confirmed by clinical and CT(n=3) or1 8F-FDG PET/CT(n=1) follow-up in 4 patients and biopsy of 1 *** integrated PET/CT image,1 8F-FDG PET was true-positive in all 5 patients with spleen *** was true-positive in 4 of the 5 patients with spleen infiltration and false-negative in 1 patients (spleen infiltration without morphology changes).The accuracies of1 8F-FDG PET and CT for evaluating the spleen infiltration were 100% and 80% at staging,*** preliminary results suggested metabolic imaging of1 8F-FDG PET/CT may be helpful in the diagnosis of spleen infiltration in B-cell NHL *** patients may benefit from1 8F-FDG PET/CT in diagnosis,when spleen infiltration without morphology changes,which may not be diagnosed exactly by conventional image.

关键词： SUV ^18F-FDG PET/CT 能级原子核物理学

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Adaptive sampling algorithm for detection of superpoints

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Science in China(Series F) 2008年第11期51卷 1804-1821页

作者： CHENG Guang GONG Jian DING Wei wu hua QIANG ShiQiang Key Lab of Jiangsu Computer Network School of Computer Science and Engineering Southeast University Nanjing 210096 China

The superpoints are the sources （or the destinations） that connect with a great deal of destinations （or sources） during a measurement time interval, so detecting the superpoints in real time is very important to network security and management. Previous algorithms are not able to control the usage of the memory and to deliver the desired accuracy, so it is hard to detect the superpoints on a high speed link in real time. In this paper, we propose an adaptive sampling algorithm to detect the superpoints in real time, which uses a flow sample and hold module to reduce the detection of the non-superpoints and to improve the measurement accuracy of the superpoints. We also design a data stream structure to maintain the flow records, which compensates for the flow Hash collisions statistically. An adaptive process based on different sampling probabilities is used to maintain the recorded IP addresses in the limited memory. This algorithm is compared with the other algorithms by analyzing the real network trace data. Experiment results and mathematic analysis show that this algorithm has the advantages of both the limited memory requirement and high measurement accuracy.

关键词： superpoints detection adaptive process flow sample and hold collision compensation

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Reactive Oxygen Species Scavenging Enzymes and Down-Adjustment of Metabolism Level in Mitochondria Associated with Desiccation-Tolerance Acquisition of Maize Embryo

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Journal of Integrative Plant Biology 2009年第7期51卷 638-645页

作者： Jing-hua wu Wei-Qing Wang Song-Quan Song Hong-Yan Cheng Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

It is a well-known fact that a mature seed can survive losing most of its water, yet how seeds acquire desiccation- tolerance is not well understood. Through sampling maize embryos of different developmental stages and comparatively studying the integrity, oxygen consumption rate and activities of antioxidant enzymes in the mitochondria, the main origin site of reactive oxygen species （ROS） production in seed cells, we found that before an embryo achieves desiccation-tolerance, its mitochondria shows a more active metabolism, and might produce more ROS and therefore need a more effective ROS scavenging system. However, embryo dehydration in this developmental stage declined the activities of most main antioxidant enzymes and accumulated thiobarbituric acid-reactive products in mitochondria, and then destroyed the structure and functional integrity of mitochondria. In physiologically-matured embryos （dehydration- tolerant）, mitochondria showed lower metabolism levels, and no decline in ROS scavenging enzyme activities and less accumulation of thiobarbituric acid-reactive products after embryo dehydration. These data indicate that seed desiccation- tolerance acquisition might be associated with down-adjustment of the metabolism level in the late development stage, resulting in less ROS production, and ROS scavenging enzymes becoming desiccation-tolerant and then ensuring the structure and functional integrity of mitochondria.

关键词： desiccation-tolerance developing embryo mitochondria reactive oxygen species scavenging enzymes

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Impact of p27mt gene on transplantation model of human colorectal cancer in nude mice

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World Journal of Gastroenterology 2009年第3期15卷 369-372页

作者： Jun Chen wu-hua Ding Guang-Xin Lu Shao-Yong Xu Department of Gastroenterology Affiliated People's Hospital of Yunyang Medical College Shiyan 442000 Hubei Province China Obstetrics and Gynecology Department Affiliated Taihe Hospita of Yunyang Medical College Shiyan 442000 Hubei Province China

AIM:To investigate the inhibitory and anti-metastatic effect of mutant p27 gene(p27mt) on the growth of colorectal cancer xenografts in nude mice and its underlying ***:Inhibitory effect of p27mt gene on the growth of colorectal cancer xenografts was determined by measurement of tumor size before and after direct intratumoral injection of Ad-p27mt in a preestablished transplantation model of human colorectal cancer in nude *** cycle and apoptosis were detected by flow cytometry performed on single-cell suspension from an isolated *** of MMP-9 in tumor tissue was detected by ***:The average sizes of transplantation tumors were 1.94 ± 0.67 cm3,2.75 ± 0.83 cm3 and 3.01 ± 0.76 cm3 in the Ad-p27mt,Ad-LacZ and control groups,respectively(P < 0.05).The average proliferation rates were 37.34% ± 1.45%,53.16% ± 3.27% and 54.48% ± 2.43%,in the Ad-p27mt,Ad-LacZ and control groups,respectively(P < 0.05).The average apoptosis rates were 19.79% ± 3.32%,6.38% ± 4.91% and 7.25% ± 5.20% in the Ad-p27mt,Ad-LacZ and control groups,respectively(P < 0.01).The average MMP-9 expression rates were 20%,75% and 66.7% in the Ad-p27mt,Ad-LacZ and control groups,respectively(P < 0.01).CONCLUSION:p27mt inhibits the growth of transplanted tumor by blocking the proliferation of cancer xenografts and by promoting apoptosis of transplantated tumor cells,as well as decrease transpl-anted tumor metastasis.

关键词： Colorectal cancer p27mt gene Nude mice MMP-9

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