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Chinese Journal of Natural Medicines 2014年第5期12卷 367-372页

作者： hu gao-sheng JIA Jing-Ming Doh Hoon Kim School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Joint Laboratory of Shenyang Pharmaceutical University Dong-A University College of Life Science and Natural Resources Dong-A University

AIM: To investigate the effects of feeding phenylalanine(Phe) and tyrosine(Tyr) on the accumulation of total phenolic compounds and four phenylethanoid glycosides(PeGs) to a cell suspension culture of the parasitic plant Cistanche deserticola. METHOD: A cell suspension culture of C. deserticola was established and precursors of different concentrations were fed. In each group, the cell was sampled at the 24th day after inoculation. The content of total phenolic compounds and four PeGs compounds were determined using the Folin-Ciocalteu method and an HPLC method, respectively. RESULTS: In the Phe fed cells, the maximum PeGs yield was achieved when Phe was fed at 1.5 mmol·L-1 and the yield reached 1.13 times the control cell concentration. In the Tyr fed cells, the maximum yield of PeGs was 1.60 times of control when 0.75 mmol·L-1 Tyr was fed to the cells. Furthermore, it was found that the salidroside yield was 4.01 times of control group when 5 mmol·L-1 Tyr was fed. CONCLUSION: Tyr is a better precursor for PeGs accumulation compared with Phe, and the rate limiting enzymes might be involved in the Tyr branch.

关键词： Cistanche deserticola Cell suspension culture Phenylalanine Tyrosine Precursor feeding RP-HPLC Salidroside Biosynthesis Phenylethanoid glycosides

Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats

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Chinese Journal of Natural Medicines 2017年第4期15卷 265-270页

作者： hu gao-sheng huANG Chong-Gui ZHANG Yang XIAO Wei JIA Jing-Ming School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Pharmaceutical Research Center Jiangsu Kanion Pharmaceutical Co. Ltd. The Sixth Traditional Chinese Medicines Factory Zhongxin Pharmaceutical Group Ltd.

Poria cocos(Schw.) Wolf, an important medicinal and food fungus, is well known in East Asia. Due to growing market demand, long cultivation period, and consumption of pine trunk during cultivation, developing alternative methods for producing P. cocos and/or its active components is of interest. In the present study, the effects of different culture methods on biomass and accumulation of four triterpenoids were investigated. The ethanol extract of fermented mycelium(EFM) was orally administered to rats. Urine output and concentrations of electrolytes(Na^+, K^+, and Cl^-) were measured. Our results showed that mycelia grew better under continuous shaking culture condition(7.5 g DW·L^(-1)), and higher triterpenoid levels were accumulated in two-stage culture(112 mg·L-1, 2.03%). The optimal starting time of static culture for triterpenoid yield was 4^(th) d after shaking culture. Single administration of middle and high dose of EFM significantly increased urine output, Na^+ and Cl^- excretion, and Na^+/K^+ ratio. These results suggested that ethanol extract of cultured mycelia showed significant diuretic activity in rats and two-stage culture of P. cocos could be an alternative way to produce mycelia and triterpenoids.

关键词： Poria cocos Two-stage culture Diuretic activity Triterpenoids Content determination

Molecular cloning and characterization of three phenylalanine ammonia-lyase genes from Schisandra chinensis

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Chinese Journal of Natural Medicines 2022年第7期20卷 527-536页

作者： FAN San-Peng CHEN Wei WEI Jiang-Chun gao Xiao-Xu YANG Yong-Cheng WANG An-hua hu gao-sheng JIA Jing-Ming School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China

Phenylalanine ammonia-lyase(PAL),which catalyzes the conversion from L-phenylalanine to trans-cinnamic acid,is a well-known key enzyme and a connecting step between primary and secondary metabolisms in the phenylpropanoid biosynthetic pathway of plants and *** chinensis,a woody vine plant belonging to the family of Magnoliaceae,is a rich source of dibenzocyclooctadiene lignans exhibiting potent ***,the functional role of PAL in the biosynthesis of lignan is relatively limited,compared with those in lignin and flavonoids ***,it is essential to clone and characterize the PAL genes from this valuable medicinal *** this study,molecular cloning and characterization of three PAL genes(ScPAL1−3)from *** was carried *** were cloned using RACE *** sequence analysis of the three ScPALs was carried out to give basic characteristics followed by docking *** order to determine their catalytic activity,recombinant protein was obtained by heterologous expression in pCold-TF vector in Escherichia coli(BL21-DE3),followed by Ni-affinity *** catalytic product of the purified recombinant proteins was verified using RP-HPLC through comparing with standard *** optimal temperature,pH value and effects of different metal ions were ***,Kcat and Km values were determined under the optimal *** expression of three ScPALs in different tissues was also *** work provided essential information for the function of ScPALs.

关键词： Schisandra chinensis Phenylalanine ammonia-lyase Molecular cloning and characterization Kinetics

Epidemiology and current management of cerebrovascular disease in China

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Journal of Geriatric Cardiology 2024年第5期21卷 465-474页

作者： sheng-Shou hu The Writing Committee of the Report on Cardiovascular Health and Diseases in China

The Annual Report on Cardiovascular Health and Diseases in China(2022)intricate landscape of cardiovascular health in *** connection with the previous section,this fifth section of the report continues the dissection on the manage-ment of cardiovascular diseases(CVD).Cerebrovascular disease is the leading cause of death and loss of healthy life among Chinese *** on the results of GBD 2019,from 1990 to 2019,the years of life lost due to premature death caused by stroke showed a decreasing trend,while the years lived with disability still increased *** present,national mortal-ity surveillance system can provide national and provincial representative annual death data on cerebrovascular disease,but the national representative data on some other important epidemiological indicators(such as incidence,prevalence,disability rate,and case fatality rate)are scarce in *** the construction of large cohort population and extension of follow-up time,re-search on stroke-related risk factors is increasing,providing a basis for the prevention and control of risk *** to limited large-scale population-based intervention studies,there is a lack of epidemiological evidence to transform into feasible interven-tion strategies and *** recent years,great progress in endovascular treatment for basilar-artery occlusion has been achieved in China,but there is still much room for improvement of guideline-based anticoagulant treatment and lipid-lowering treatment,as well as standardized diagnosis and treatment among patients with ischemic stroke.

关键词： prevention diagnosis Epidemiology

Epidemiology and current management of cardiovascular disease in China

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Journal of Geriatric Cardiology 2024年第4期21卷 387-406页

作者： sheng-Shou hu the Writing Committee of the Report on Cardiovascular Health and Dis-eases in China Fuwai Hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

The Annual Report on Cardiovascular Health and Diseases in China(2022) intricate landscape of cardiovascular health in China. This is the fourth section of the report with a specific focus on epidemiology and current management of cardiovascular disease(CVD) in China. This section of the report highlights the epidemiological trends of CVD in China. It reveal a concerning rise in prevalence, with approximately 330 million affected individuals, including significant numbers with stroke, coronary artery disease(CAD), heart failure, and other conditions. CVD stands as the primary cause of mortality among both urban and rural populations, accounting for nearly half of all deaths in 2020. Mortality rates are notably higher in rural areas compared to urban centers since 2009. While age-standardized mortality rates have decreased, the absolute number of CVD deaths has increased, primarily due to population aging. Ischemic heart disease, hemorrhagic and ischemic strokes are the leading causes of CVD-related deaths. Notably, the burden of atherosclerotic cardiovascular disease has risen substantially, with atherosclerotic cardiovascular disease-related deaths increasing from 1990 to 2016. The incidence of ischemic stroke and ischemic heart disease has shown similar increasing trends over the past three decades. CAD mortality, particularly acute myocardial infarction, has been on the rise, with higher mortality rates observed in rural areas since 2016. The prevalence of CAD has increased significantly, with over 11 million patients identified in 2013. Studies assessing hospital performance in managing acute coronary syndrome reveal gaps in adherence to guideline-recommended strategies, with disparities in care quality across hospitals. However, initiatives like the China Patient-centered Evaluative Assessment of Cardiac Events(PEACE)-Retrospective AMI Study and the Improving Care for Cardiovascular Disease in China-Acute Coronary Syndrome(CCC-ACS) project aim to improve patien

关键词： epidemiology landscape deaths

Influence of the Acoustic Liner in Large Eddy Simulation of Longitudinal Thermoacoustic Instability in a Model Annular Combustor

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Journal of Thermal Science 2024年第2期33卷 710-724页

作者： MENG sheng ZHANG Man gao Yi AECC Commercial Aircraft Engine Co. Ltd.Shanghai200241China School of Aeronautics and Astronautics Shanghai Jiao Tong UniversityShanghai200240China

Although extensive efforts have been made to dampen the thermoacoustic instability,successfully controlling the pressure oscillations in modern gas turbines or aeroengines remains *** influence of the acoustic liner on the longitudinal thermoacoustic mode in a model annular combustor is investigated by Large Eddy Simulation(LES) in this *** result of the self-excited longitudinal thermoacoustic instability without the liner agrees well with the frequency and acoustic analysis of the pressure mode based on experimental *** different bias flow velocities of the liner located downstream of the combustor are then *** results reveal that the existence of the liner influences not only the acoustic field but also the flow *** the bias velocity is large,it leads to intense turbulence-induced fluctuations,and the pressure oscillation is modulated *** shows that the weak coupling between flow and pressure oscillations plays a significant role in the onset of the intermittency of a thermoacoustic *** on the dynamic analysis of the thermoacoustic system with the acoustic liner,this intermittency is caused by the influence of the flow field on the flame-acoustic ***,a low-order modeling method based on Van der Pol(VdP) oscillator with additive stochastic forcing is conducted to reproduce the evolving dynamics of the thermoacoustic *** the numerical cases demonstrated in this work are relatively simpler than those in a practical combustion system,the results are helpful for us to understand the effect of the acoustic liner and show the attractive potential to apply this device to suppress thermoacoustic instability.

关键词： thermoacoustic instability acoustic liner Large Eddy Simulation low-order modeling

Influencing Factors on Cardiovascular Health in China

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Journal of Geriatric Cardiology 2024年第1期21卷 4-33页

作者： sheng-Shou hu 无 Fuwai Hospital Chinese Academy of Medical Sciences BeijingChina the Writing Committee of the Report on Cardiovascular Health and Diseases in China

The Annual Report on Cardiovascular Health and Diseases in China(2022)intricate landscape of cardiovascular health in *** is the first section of the report,which dissects influential factors across diverse *** investigation identifies tobacco use as a paramount concern,portraying China as the global epicenter of tobacco *** smoking,exacerbated by second-hand smoke exposure,emerges as a critical and preventable risk factor,contributing to a surge in attributable deaths over the past three *** the realm of dietary nutrition,the study discerns an overall improvement,yet discerns worrisome deviations,notably an escalating fat intake surpassing recommended *** shifting dietary structure reveals diminished consumption of cereals and vegetables juxtaposed with an uptick in animal foods,while excessive intake of cooking oil and salt persists,straying substantially from endorsed *** exploration of physical activity patterns unfolds a nuanced *** trends are observed among students,with concerns arising from sedentary behaviors and inadequate adherence to recommended *** analysis spans a trajectory of declining physical activity in Chinese adults,coupled with an alarming surge in sedentary leisure time,ultimately linking these factors to heightened risks of cardiovascular diseases and increased *** examination of overweight and obesity trends uncovers a relentless upward trajectory,projecting substantial prevalence by *** prevalence rates underscore the imperative for targeted interventions to curtail this burgeoning health crisis,with the anticipated prevalence extending to nearly two-thirds of the adult *** factors,notably depression,constitute an integral facet of cardiovascular *** rates among patients with coronary artery disease and acute myocardial infarction underscore the intricate interplay between mental health and cardiovascular

关键词： Influencing smoke linking

Salp Swarm Incorporated Adaptive Dwarf Mongoose Optimizer with Lévy Flight and Gbest-Guided Strategy

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Journal of Bionic Engineering 2024年第4期21卷 2110-2144页

作者： Gang hu Yuxuan Guo Guanglei sheng Department of Applied Mathematics Xi’an University of TechnologyXi’an710054People’s Republic of China School of Computer Science and Engineering Xi’an University of TechnologyXi’an710048People’s Republic of China Department of Electronics and Information Engineering Bozhou UniversityBozhou236800People’s Republic of China

In response to the shortcomings of Dwarf Mongoose Optimization(DMO)algorithm,such as insufficient exploitation capability and slow convergence speed,this paper proposes a multi-strategy enhanced DMO,referred to as ***,we propose an improved solution search equation that utilizes the Gbest-guided strategy with different parameters to achieve a trade-off between exploration and exploitation(EE).Secondly,the Lévy flight is introduced to increase the diversity of population distribution and avoid the algorithm getting stuck in a local *** addition,in order to address the problem of low convergence efficiency of DMO,this study uses the strong nonlinear convergence factor Sigmaid function as the moving step size parameter of the mongoose during collective activities,and combines the strategy of the salp swarm leader with the mongoose for cooperative optimization,which enhances the search efficiency of agents and accelerating the convergence of the algorithm to the global optimal solution(Gbest).Subsequently,the superiority of GLSDMO is verified on CEC2017 and CEC2019,and the optimization effect of GLSDMO is analyzed in *** results show that GLSDMO is significantly superior to the compared algorithms in solution quality,robustness and global convergence rate on most test ***,the optimization performance of GLSDMO is verified on three classic engineering examples and one truss topology optimization *** simulation results show that GLSDMO achieves optimal costs on these real-world engineering problems.

关键词： Dwarf mongoose optimization algorithm Gbest-guided Lévy flight Adaptive parameter Salp swarm algorithm Engineering optimization Truss topological optimization

Disorders of cardiac rhythm in China

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Journal of Geriatric Cardiology 2024年第7期21卷 703-712页

作者： sheng-Shou hu The Writing Committee of the Report on Cardiovascular Health and Diseases in China

The Annual Report on Cardiovascular Health and Diseases in China(2022)intricate landscape of cardiovascular health in *** connection with the previous section,this seventh section of the report offers a comprehensive analysis of dis-orders of heart rhythm in *** 2021,China has achieved significant development and gratifying results in many aspects of the field of *** bundle branch pacing(LBBP),as an emerging pacing technique originating from China,has received widespread *** research results have emerged on its indications,surgical procedures,clinical evaluation,and com-parison with other pacing *** feasibility,effectiveness,and safety have been basically verified,but its long-term pro-gnosis still needs further confirmation from larger samples and longer follow-up time research *** pacemakers have begun to be used in a wider range of clinical applications,and related large sample cohort studies have been *** addi-tion,there are also noteworthy new achievements in the fields of pacemaker remote programming,anticoagulation and radiofre-quency catheter ablation(RFCA)therapy for atrial fibrillation,and implantable cardioverter defibrillator prevention of sudden cardiac *** terms of clinical practice,due to COVID-19 pandemic,the number of RFCA procedures and other device im-plantations in China has fluctuated,but it has gradually recovered since 2020.

关键词： cardiac prevention landscape

Trajectory tracking guidance of interceptor via prescribed performance integral sliding mode with neural network disturbance observer

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Defence Technology（防务技术） 2024年第2期32卷 412-429页

作者： Wenxue Chen Yudong hu Changsheng gao Ruoming An School of Astronautics Harbin Institute of TechnologyHarbin 150001China

This paper investigates interception missiles’trajectory tracking guidance problem under wind field and external disturbances in the boost ***,the velocity control in such trajectory tracking guidance systems of missiles is *** our contribution,the velocity control channel is designed to deal with the intractable velocity problem and improve tracking *** global prescribed performance function,which guarantees the tracking error within the set range and the global convergence of the tracking guidance system,is first proposed based on the traditional ***,a tracking guidance strategy is derived using the integral sliding mode control techniques to make the sliding manifold and tracking errors converge to zero and avoid ***,an improved switching control law is introduced into the designed tracking guidance algorithm to deal with the chattering problem.A back propagation neural network(BPNN)extended state observer(BPNNESO)is employed in the inner loop to identify *** obtained results indicate that the proposed tracking guidance approach achieves the trajectory tracking guidance objective without and with disturbances and outperforms the existing tracking guidance schemes with the lowest tracking errors,convergence times,and overshoots.

关键词： BP network neural Integral sliding mode control(ISMC) Missile defense Prescribed performance function(PPF) State observer Tracking guidance system