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Agricultural Sciences 2020年第3期11卷 260-277页

作者： Rababe Es-Soufi houda tahiri Latifa Azaroual Aicha El Oualkadi Patrick Martin Alain Badoc Ahmed Lamarti Laboratory of Plant Biotechnology Biology DepartmentFaculty of SciencesAbdelmalek Essaadi UniversityTetouanMorocco INRA-CRRA-Tangier TangerMorocco Water Laboratory Environmental Studies and Analyzes(L2EAE)Department of ChemistryFaculty of ScienceAbdelmalek Essaadi UniversityTétouanMorocco Artois university UniLaSalleULR7519-Transformations&Agroressources UnitBéthuneFrance MIB(Molecules of Biological Interest) Research Unit(Enology EA 4577USC 1366 INRA)UFR of Pharmaceutical SciencesBordeaux UniversityISVV(Institute of Vine and Wine Sciences)BordeauxFrance

The increasingly strict regulation of the use of phytosanitary products and the will of the populations to move towards sustainable development allow bio-sourced products and more particularly for biocontrol in the field of pesticides to progress. The study carried out here concerns the evaluation of the potency of Bacillus amyloliquefaciens Bc2 and Trichoderma harzianum to fight against strawberry cultures infected with anthracnose (Fragaria × ananassa Duch.). The studies were carried out in the laboratory and in the field. The results indicate that B. amyloliquefaciens Bc2 and Trichoderma harzianum TR are effective for the biological control of anthracnose, gray mold and powdery mildew on strawberries grown in field conditions. The increase in plant size and the number of fruits produced with these control agents has also been observed.

关键词： Biocontrol Bacillus amyloliquefaciens Trichoderma harzianum Strawberry Anthracnose Colletotrichum acutatum

Effect of Different Concentrations of the Suspensions of *** and *** on the Development of ***

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Advances in Microbiology 2020年第3期10卷 72-81页

作者： Rababe Es-Soufi houda tahiri Latifa Azaroual Aicha El Oualkadi Patrick Martin Alain Badoc Ahmed Lamarti Laboratory of Plant Biotechnology Biology DepartmentFaculty of SciencesAbdelmalek Essaadi UniversityTetouanMorocco INRA-CRRA-Tangier 78.bd.Sidi Mohamed Ben AbdellahTangerMorocco Water Laboratory Environmental Studies and Analyzes(L2EAE)Department of ChemistryFaculty of ScienceAbdelmalek Essaadi UniversityTétouanMorocco Artois University UniLaSalleULR7519-Transformations&Agroressources UnitBéthuneFrance MIB(Molecules of Biological Interest) Research Unit(Enology EA 4577USC 1366 INRA)UFR of Pharmaceutical SciencesBordeaux UniversityISVV(Institute of Vine and Wine Sciences)BordeauxFrance

The effect of nine isolates of Bacillus amyloliquefaciensand one strain of Trichoderma harzianum, TR, on mycelial growth and germination of Colletotrichum acutatum were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations. Results showed that one Bacillus isolates (Bc2) and TR were more effective at the lower concentration tested (3 × 105 CFU/ml and 105 conidia/ml).

关键词： Biocontrol Concentration Inoculum Bacillus amyloliquefaciens Trichoderma harzianum Colletotrichum acutatum

In Vitro Antagonistic Activity of Trichoderma harzianum and Bacillus amyloliquefaciens against Colletotrichum acutatum

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Advances in Microbiology 2020年第3期10卷 82-94页

作者： Rababe Es-Soufi houda tahiri Latifa Azaroual Aïcha El Oualkadi Patrick Martin Alain Badoc Ahmed Lamarti Laboratory of Plant Biotechnology Biology DepartmentFaculty of SciencesAbdelmalek Essaadi UniversityTetouanMorocco INRA-CRRA-Tangier 78.bd.Sidi Mohamed Ben AbdellahTangerMorocco Water Laboratory Environmental Studies and Analyzes(L2EAE)Department of ChemistryFaculty of ScienceAbdelmalek Essaadi UniversityTétouanMorocco Artois University UniLaSalleULR7519-Transformations&Agroressources UnitBéthuneFrance MIB(Molecules of Biological Interest) Research Unit(Enology EA 4577USC 1366 INRA)UFR of Pharmaceutical SciencesBordeaux UniversityISVV(Institute of Vine and Wine Sciences)BordeauxFrance

Biological control of plant diseases makes extensive use of the antagonists. The effectiveness of the control depends on the choice of an effective antagonist strains from criteria of implying a good knowledge of biological peculiarities of the material used. In this study, the antagonistic activities of nine isolates of Bacillus amyloliquefaciens and one isolate of Trichoderma harzianum were tested in vitro against seven strains of Colletotrichum acutatum, the causal agent of strawberry anthracnose. It was found that T. harzianum and B. amyloliquefaciens strains showed the ability to inhibit the mycelial growth of the pathogen by dual culture technique by more than 50 percent. The nonvolatile substances produced by the antagonists showed high inhibition percentages that are more than 99 percent, meanwhile their volatile compounds inhibited the mycelial growth of C. acutatum strains with low inhibition percentages starting from 30 percent except the substances produced by B. amyloliquefaciens Bc2 that showed a high inhibition percentage of more than 70 percent.

关键词： Biocontrol Antagonism Bacillus amyloliquefaciens Trichoderma harzianum Anthracnose Colletotrichum acutatum

Evaluation of Plant Growth Promoting Ability of Bacillus amyloliquefaciens Bc2 and Trichoderma harzianum TR In Vivo

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Agricultural Sciences 2020年第3期11卷 247-259页

作者： Rababe Es-Soufi houda tahiri Aicha El Oualkadi Latifa Azaroual Patrick Martin Alain Badoc Ahmed Lamarti Laboratory of Plant Biotechnology Biology DepartmentFaculty of SciencesAbdelmalek Essaadi UniversityTetouanMorocco INRA-CRRA-Tangier TangerMorocco Water Laboratory Environmental Studies and Analyzes(L2EAE)Department of ChemistryFaculty of ScienceAbdelmalek Essaadi UniversityTétouanMorocco Artois University UniLaSalleULR7519-Transformations&Agro Resources UnitBéthuneFrance MIB(Molecules of Biological Interest) Research Unit(Enology EA 4577USC 1366 INRA)UFR of Pharmaceutical SciencesBordeaux UniversityISVV(Institute of Vine and Wine Sciences)BordeauxFrance

Bacillus amyloliquefaciens Bc2 and Trichderma harzianum TR were used to evaluate their growth-promoting activity on cultivated strawberries, under laboratory and field conditions, and we have noticed that the percentage of achene germination is important for ones treated with TR (=97%) followed by those treated with Bc2 strain (=90%) and the control (=84%). Inoculations on field showed that on untreated soil with insecticide, TR is effective and allows the development of plants and extends the duration of flowering and fruiting. On treated soil, Bc2 clearly promotes the growth and development of strawberry seedlings and its role as plant growth promoting microorganisms has been proved.

关键词： Plant Growth Promoting Strawberry PGPM Bacillus amyloliquefaciens Trichoderma harzianum

Micropropagation of the Moroccan Endemic Plant Thymus broussonetiiBoiss. with Aromatic-Medicinal Value and Conservation Concern

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American Journal of Plant Sciences 2020年第6期11卷 913-938页

作者： Zineb Nejjar El Ansari Ibtissam Boussaoudi Rajae Benkaddour houda tahiri Aïcha El Oualkadi Alain Badoc Patrick Martin Ahmed Lamarti Laboratory of Plant Biotechnology Biology Department Faculty of Sciences Abdelmalek Essaadi University Tetouan Morocco Genetic Improvement of Plants Laboratory/National Institute of Agronomic Research (INRA) Tangier Morocco Axe MIB (Molécules d’Intérêt Biologique) Unité de Recherche &#338 nologie UFR des Sciences Pharmaceutiques USC INRAE 1366-EA 4577 ISVV (Institut des Sciences de la Vigne et du Vin) Villenave-d’Ornon France Université d’Artois UniLaSalle ULR7519-Unité Transformations & Agroressources Béthune France

Micropropagation from shoot tips and nodal segments was carried out for the conservation and domestication of spontaneous Moroccan thyme, Thymus broussonetii Boiss. subsp. broussonetii (endemic threatened). The mineral composition of the culture medium, as well as the succession of different growth regulators, influenced the in vitro growth of this species. Sterilized achenes of T. broussonetii were able to germinate on an agar medium containing Gautheret macronutrients with a rate of 25% and a degree of contamination of less than 4%. Shoot apices of 15-day seedlings (two cotyledon leaves) were cultivated on SD + 0.46 μM Kin medium and the explants obtained were transplanted every month. Six macronutrients (MS, B5, SH, SD, MSm and N30K) were tested and N30K was chosen for the following experiments. Seven cytokinins (Kin, BAP, 2iP, DPU, adenine, Zeat and TDZ) at 0.46, 0.93 and 2.32 μM/l were evaluated and the addition of 0.93 μM adenine to N30K medium favored significantly the induction of buds and the elongation of explants. Three polyamines (putrescine, spermidine and spermine) at 2, 5, 10 and 20 μM/l were tested. A better multip

关键词： Thymus broussonetii Micropropagation Shoot Tip Nodes Macronutrients Cytokinins Polyamines Auxins Prototypes Conservation

Effect of Macronutrients, Cytokinins and Auxins, on in VitroOrganogenesis of Thymus vulgarisL.

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American Journal of Plant Sciences 2019年第9期10卷 1482-1502页

作者： Zineb Nejjar El Ansari Amina El Mihyaoui Ibtissam Boussaoudi Rajae Benkaddour Ouafaa Hamdoun houda tahiri Alain Badoc Aicha El Oualkadi Ahmed Lamarti Laboratory of Plant Biotechnology Biology Department Faculty of Sciences Abdelmalek Essaadi University Tetouan Morocco Genetic Improvement of Plants Laboratory /National Institute of Agronomic Research (INRA) Tangier Morocco Axe MIB (Molécules d’Intéret Biologique) Unité de Recherche (Enologie EA 4577 USC 1366 INRA) UFR des Sciences Pharma-ceutiques Université de Bordeaux ISVV (Institut des Sciences de la Vigne et du Vin) Bordeaux France

The present study reports an efficient protocol for in vitro propagation of Thymus vulgaris L., an aromatic and medicinal plant in Morocco. Initially, we performed in vitro multiplication of Thymus vulgaris explants existing in the laboratory and obtained from micropropagation by shoot tip culture. Afterwards, we have evaluated the effect of six macronutrients. After that, seven cytokinins (Kin, BAP, 2iP, DPU, Adenine, Zeatine and TDZ) in three different concentrations (0.46, 0.93, 2.32 μM) have been evaluated to optimize cultures multiplication and elongation. Moreover, the effect of three auxins (IAA, IBA and NAA) at 0.57 μM, combined to 4 cytokinins (Kin, BAP, DPU and Ad.) at 0.46 μM, on shoot rooting has been studied. Thereby, MS medium has been proved the most favorable for plantlets growing. Also, we found that the addition of certain cytokinins, specifically 0.46 Kin, 0.46 and 0.93 BAP, 0.46 2iP, 0.46 DPU, 0.46 Ad. and 0.46 Zeat., ensures better multiplication and growth of vitroplants. In addition, multiplication and rooting of cultures were well optimized after addition 0.46 Kin + 0.57 IAA or NAA, 0.46 DPU + 0.57 IBA and 0.46 Ad. + 0.57 IBA combinations to the culture medium. Lastly, plantlets with roots were successfully acclimatized to ex-vitro conditions and these latter served as a source to establish in vitro culture again.

关键词： Thymus vulgaris L. Macronutrients Cytokinins Auxins In Vitro Propagation

*** of beta-adrenergic receptor in choroidal neovascularization

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Annals of Eye Science 2018年第1期 439-439页

作者： houda tahiri Samy Omri Isabelle Lahaie Sylvain Chemtob Research Center Hôpital Maisonneuve-Rosemont Universitéde MontréalMontréalQCCanada Research Center CHU Sainte-Justine Universitéde MontréalMontréalQCCanada Department of Pharmacology Universitéde MontréalMontréalQCCanada Department of Ophthalmology Universitéde MontréalMontréalQCCanada Department of Pediatrics Universitéde MontréalMontréalQCCanada

Background:We investigated the role of beta-adrenergic receptor(B-AR)on choroidal neovascularization(CNV)in an animal model of age-related macular degeneration in ***:The angiogenic effect of the B-AR was evaluated in retinal pigment epithelium(RPE)-choroid explants from C57Bl6 mice stimulated with propranolol or isoproterenol(10μM)(respectively antagonist and agonist of the B-AR)during 24 ***,a classic choroidal neovascularization(CNV)model induced by laser burn in C57Bl6 mice(8 weeks)was used to assess the anti-angiogenic effect of *** this experiment,mice were treated with intraperitoneal propranolol(6 mg/kg/d)or vehicle(saline solution)daily for 10 days,starting on day 4 after laser burn and until sacrifice(day 14).Immunostaining analysis on retinal flatmounts and cryosections were performed to determine the surface of CNV,the distribution of B-AR and the number and morphology of microglia/macrophages associated with *** explore if the antiangiogenic effect of propranolol involved the modulation of the inflammatory microenvironment associated with CNV,we used RPE primary cells,J774 macrophages cell line and polarized M1 and M2 bone marrow-derived macrophage(BMDM).Choroidal explants treated with conditioned media(CM)from J774 or polarized M1/M2 BMDM pre-treated with propranolol to confirm the anti-angiogenic effect of *** of angiogenic factors was evaluated by RT PCR and ***:The expression and distribution of the B-1,B-2 and B-3 adrenergic receptors were localized in the choroid and RPE *** stimulation of RPE-choroid explants with isoproterenol increased CNV compared to vehicle,while propranolol decreased *** vivo,propranolol inhibited significantly the levels of VEGF and CNV growth in laser burn model compared to the ***,the treatment with propranolol decremented the number of activated(amoeboid shape)microglia/macrophages but surprisingly,the number of non-activated microglia

关键词： Choroidal neovascularization(CNV) macrophages beta-adrenergic receptor(B-AR)

***-standing choroidal thinning in oxygen-induced retinopathy

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Annals of Eye Science 2018年第1期 449-449页

作者： Tianwei Ellen Zhou Tang Zhu houda tahiri Samy Omri JoséCarlos Rivera Isabelle Lahaie Cheri Deal Stanley Nattel Sylvain Chemtob Department of Pharmacology McGill UniversityMontréalQCCanada Department of Pharmacology Universitéde MontréalMontréalQCCanada Research Center Hôpital Maisonneuve-Rosemont Universitéde MontréalMontréalQCCanada Research Center CHU Sainte-Justine Universitéde MontréalMontréalQCCanada Department of Pediatrics Universitéde MontréalMontréalQCCanada Department of Ophthalmology Universitéde MontréalMontréalQCCanada

Background:Retinopathy of prematurity(ROP),the most common cause of blindness in premature infants,has long been associated with pathologic retinal ***,recent studies reveal choroidal involution in adolescent patients formerly afflicted with *** have recently demonstrated that choroidal thinning occurs early in retinopathy and persists into *** retinal vessels,the damaged choroidal vasculature in ROP is incapably to ***,we investigated the molecular mechanism implicated in the lack of choroidal repair in ischemic ***:The oxygen-induced retinopathy(OIR)model was *** Sprague-Dawley(albino)or Long-Evans rats(pigmented)rats were placed under oxygen concentration which cycles at 50%±1%or 10%±1%every 24 hours(hr)from postnatal day(P)0 to *** P14,all rats were returned to room *** blotting and qPCR were used to quantify protein and RNA abundances,*** Dual-Luciferase®Reporter Assay System was used to confirm microRNA(miRNA)-mRNA ***:We detected a substantial oxidative stress in retinal pigment epithelium(RPE)and choroidal tissue,accompanied by a drastic reduction in insulin-like growth factor 1 receptor(IGF1R),a critical player in post-injury *** mechanism of decreasing IGF1R involves the over-activation of the p53 tumor suppressor that regulates miRNA let-7b,which subsequently silences Igf1r mRNA in the RPE/choroid complex of OIR *** reporter assay confirmed that let-7b directly targets Igf1r mRNA at its 3’untranslated region(UTR).Indeed,silencing p53 resulted in a decreased let-7b expression,and re-established IGF1R abundance that promoted choroidal ***:Together,this study sets forth new mechanistic notion by uncovering the novel p53/let-7b/IGF1R axis;timely intervention of this pathway facilitates healthy choroidal *** investigations on anti-angiogenic miRNAs can better our understanding

关键词： Choroid retinopathy of prematurity(ROP) p53 microRNA

AB035. Lactate receptor GPR81 modulates epigenetic modification in the subretina

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Annals of Eye Science 2018年第1期 441-441页

作者： Xiaojuan Yang Raphael Rouget Tang Zhu Christiane Quiniou Shasha Lv Suna Jung Francois Duhamel houda tahiri Samy Omri Baraa Noueihed Xin Hou JoséCarlos Rivera Mathieu Nadeau-Vallée Pierre Lachapelle Sylvain Chemtob Department of Pharmacology McGill UniversityMontréalQCCanada Department of Pharmacology Universitéde MontréalMontréalQCCanada Research Center CHU Sainte-Justine Universitéde MontréalMontréalQCCanada Department of Ophthalmology and Neurology-Neurosurgery Montréal Children’s Hospital-Research InstituteMcGill UniversityMontréalQCCanada Research Center Hôpital Maisonneuve-Rosemont Universitéde MontréalMontréalQCCanada Department of Ophthalmology Universitéde MontréalMontréalQCCanada Department of Pediatrics Universitéde MontréalMontréalQCCanada

Background:Retinal pigment epithelium(RPE)is vital for the homeostasis of the subretina including photoreceptors and ***,our previous results suggested that the recently discovered lactate receptor GPR81 is abundantly expressed in *** date,only one previous study has shown that activating GPR81 could enhance DNA repair by activating ***,we investigated whether GPR81 exhibits epigenetic modification in the subretina by using GPR81−/−***:GPR81−/−mice and wide type littermates were generated on a background of C57BL/6J *** thicknesses of their choroid were evaluated by ***,Q-PCR,western blot and choroid sprout assay were *** vitro,primary retinal pigment epithelium(pRPE)cells were isolated from mice,and cultured for ***:The thickness of choroid was reduced in GPR81−/−mice compared to GPR81+/+mice,suggesting that GPR81 is important for the integrity of *** the choroid sprout assay,lactate treated RPE/choroid complex showed a significant increase in angiogenesis compared to controls while lactate treated KO RPE/choroid complex showed no difference compared to their *** Q-PCR,most of the genes screened elevated their expression in GPR81−/−mice compared to WT mice,suggesting epigenetic modification may exist,which were confirmed by histone acetylation and HDACs activity ***:Taking together,the lactate receptor GPR81 in RPE is very important for maintaining homeostasis of the *** novel discovery sheds new light on the relationship between metabolism and epigenetic modification.

关键词： Subretina GPR81 epigenetic modification

Off-shell BRST-VSUSY Superalgebra for D = 4 BF Theories in Superconnection Formalism

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Communications in Theoretical Physics 2007年第2X期48卷 309-312页

作者： A. Aidaoui M. tahiri Laboratoire de Physique Théorique Universitd d'Oran Es-Senia 31100 Oran Algeria Centre Universitaire de Béchar Institut des Sciences Exactes B.P. 417 08000 Béchar Algeria

We propose the supereonneetion formalism to construct the off-shell BRST-VSUSY superalgebra for D = 4 BF theories. The method is based on the natural introduction of physical fields as well as auxiliary fields via supereon- neetions and their associated supereurvatures defined on a superspaee. We also give a prescription to build the off-shell BRST-VSUSY exact quantum action.

关键词： superconnection off-shell BRST-VSUSY superalgebra BF theories