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反相胶束介质对2,3-二氨基吩嗪的荧光增敏作用研究

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高等学校化学学报 1999年第7期20卷 1031-1035页

作者：黄应平蔡汝秀黄厚评武汉大学化学系

研究了反相胶束介质丁二酸双（２－乙基己基）酯磺酸钠（ＡＯＴ）／正辛烷（ｎ－ｏｃｔａｎｅ）对漆酶催化氧化邻苯二胺的产物２，３－二氨基吩嗪（ＤＡＰ）光谱性质的影响．试验表明，ＡＯＴ／ｎ－ｏｃｔａｎｅ对ＤＡＰ吸光性质稍有增... 详细信息

研究了反相胶束介质丁二酸双（２－乙基己基）酯磺酸钠（ＡＯＴ）／正辛烷（ｎ－ｏｃｔａｎｅ）对漆酶催化氧化邻苯二胺的产物２，３－二氨基吩嗪（ＤＡＰ）光谱性质的影响．试验表明，ＡＯＴ／ｎ－ｏｃｔａｎｅ对ＤＡＰ吸光性质稍有增敏作用，而对其荧光具有强烈的增敏作用，使ＤＡＰ荧光量子产率提高近３０倍。

关键词：反相胶束介质二氨基吩嗪荧光增敏分子光谱

Functional Analysis of Multiple Transcription Factor Sites in a Regulatory Element of Human ε-Globin Gene

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 673-680页

作者： Chun-Hui hou, Jian huang, and Ruo-Lan QIAN* State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

The developmental control of the human ε-globin gene expression is mediated by transcrip- tional regulatory elements in the 5’ flanking DNA of this gene. A previously identified negative regulatory element (–3028 to –2902 bp, termed ε-NRAII) was analyzed and one putative NF-κB site and two GATA sites locate at –3004 bp, –2975 bp and –2948 bp were characterized. Electrophoresis mobility shift assay (EMSA) showed that the putative NF-κB site was specifically bound by nuclear proteins of K562 cells. Data obtained from transient transfection showed that the expression of reporter gene could be upregulated about 50% or 100% respectively when ε-NRAII was inserted upstream of the SV40 promoter or ε-globin gene proximal promoter (–177 bp to +1 bp), suggesting that ε-NRAII might not be a classic silencer. Mutation in the putative NF-κB site or in the GATA site (at –2975 bp) slightly reduced the expression of reporter gene driven by SV40 promoter or ε-globin gene proximal promoter. However, the mutation of GATA site at –2948 bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. Further mutation analysis showed that the negative effect of mutation in GATA site at –2948 bp on SV40 promoter was not affected by the mutation of the putative NF-κB site, whereas it could be abolished by the mutation of GATA site at –2975 bp. Furthermore, the mutation of both GATA sites could synergistically reduce the reporter gene activity driven by ε-globin gene proximal promoter. Those results suggested that ε- NRAII might function differently on the SV40 promoter and ε-globin gene proximal promoter.

关键词： ε-globin gene regulatory element NF-κB GATA

多值信息系统中基于粒计算的多级概念获取算法

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模式识别与人工智能 2009年第1期22卷 22-27页

作者：邱桃荣刘清黄厚宽南昌大学计算机系南昌330031 北京交通大学计算机与信息技术学院北京100044

以自身具有不确定信息的多值信息系统作为研究背景,分析研究构建领域多层次概念方法.讨论属性值的近似表示和相容信息粒的生成,提出基于粒计算从具有不确定信息的多值信息系统中获取多级概念的框架和算法.用一个实例和测试比较来说明该... 详细信息

以自身具有不确定信息的多值信息系统作为研究背景,分析研究构建领域多层次概念方法.讨论属性值的近似表示和相容信息粒的生成,提出基于粒计算从具有不确定信息的多值信息系统中获取多级概念的框架和算法.用一个实例和测试比较来说明该算法是可行有效的.

关键词：粒计算多值信息系统概念获取多级概念

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无须附加空间的数据立方体联机聚集

软件学报 2006年第4期17卷 806-813页

作者：李红松黄厚宽北京交通大学计算机与信息技术学院北京100044

以往在数据立方体上实现的联机聚集往往需要附加空间来存储联机聚集估算所需要的信息,极大地影响了数据立方体的存储和维护性能.提出了基于QC-Tree的用于范围查询处理的联机聚集PE(progressivelyestimate)算法以及它与简单聚集算法相结... 详细信息

以往在数据立方体上实现的联机聚集往往需要附加空间来存储联机聚集估算所需要的信息,极大地影响了数据立方体的存储和维护性能.提出了基于QC-Tree的用于范围查询处理的联机聚集PE(progressivelyestimate)算法以及它与简单聚集算法相结合的混合聚集算法HPE(hybridprogressivelyestimate);还提出了一种能够同时处理多个范围查询的联机聚集算法MPE(multipleprogressivelyestimate).与以往联机聚集算法不同,这些算法不需要任何附加空间,而是利用QC-Tree自身保存的聚集数据和语义关系来估算聚集结果.由于QC-Tree是一种极为高效的数据立方体存储结构,因此能够以较理想的性能实现数据立方体上的联机聚集.对算法的分析和实验结果表明,所提出的算法具有较好的性能.

关键词：数据立方体联机分析处理近似查询处理联机聚集

基于主Agent信念修正的推测计算及其资源协商

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软件学报 2005年第11期16卷 1920-1928页

作者：王黎明黄厚宽北京交通大学计算机与信息技术学院

基于假设推理(abduction-based)的推测计算(speculative computation)是在资源信息不能及时到达时,利用缺省假设进行计算的过程.在计算过程中,如果应答和信念不一致,则主Agent将修正它的信念.为了实现目标,在有限时间内使推测计算的结... 详细信息

基于假设推理(abduction-based)的推测计算(speculative computation)是在资源信息不能及时到达时,利用缺省假设进行计算的过程.在计算过程中,如果应答和信念不一致,则主Agent将修正它的信念.为了实现目标,在有限时间内使推测计算的结果更精确,主Agent要通过协商获得尽可能多的实际信息,协商是降低决策风险的主要途径.在介绍假设推理和推测计算的基本原理的基础上,提出了基于时间约束的推测计算扩展框架、基于时间约束的进一步协商框架和基于信念修正的协商算法,并将进一步协商框架和协商算法嵌入到推测计算的过程中,在协商过程中赋予主Agent更强的信念修正能力.最后,在货物运输领域的实验中,证实了基于信念修正的推测计算的有效性.

关键词：推测计算信念修正假设推理协商对话对话约束时间约束

Isolated extramedullary ocular relapse of acute lymphoblastic leukemia after peripheral blood stem cell transplantation

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Chinese Medical Journal 2013年第20期126卷 3992-3993页

作者： Guo Qing huang hou-bin Pi Yu-li Liu Tian-xing Chinese Peoples Liberat Army Gen Hosp Affiliated Hosp 1 Dept Ophthalmol Beijing 100048 Peoples R China Chinese Peoples Liberat Army Gen Hosp Affiliated Hosp 1 Dept Radiat Oncol Beijing 100048 Peoples R China Chinese Peoples Liberat Army Gen Hosp Dept Ophthalmol Beijing 100853 Peoples R China

Isolated extramedullary ocular relapse of acute lymphoblastic leukemia （ALL） after allogeneic peripheral blood-stem cell transplantation （allo-P-BSCT） without concomitant involvement of the bone marrow is very rare,while the common sites of extramedullary relapse are the central nervous system, skin, bone, and breasts.^1 This is the report of isolated ocular relapse without any extra- ocular involvement of ALL after allo-PBSCT confirmed by histopathology.

关键词： acute lymphoblastic leukemia peripheral blood stem cell transplantation,extramedullao, relapse retinal detachment

Clinical characteristics and distribution of hepatitis B virus genotypes in Guangxi Zhuang population

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World Journal of Gastroenterology 2005年第41期11卷 6525-6529页

作者： Zhong-Min huang Qi-Wen huang Ya-Qin Qin Chun-He huang hou-Ji Qin Yiao-Nan Zhou Xiang Xu Chun-Lei Lu Department of Infectious Diseases The Affiliated Hospital of Youjiang Medical College for Minority Nationalities Baise 533000 Guangxi Zhuang Autonomous Region China

AIM: To investigate the distribution of HBV genotypes and their YMDD mutations in Guangxi Zhuang population, China, and to study the relationship between HBV genotypes and clinical types of HB, ALT, HBV DNA, HBe system as well as the curative effect of Lamivudine (LAM) on hepatitis ***: A total of 156 cases were randomly chosen as study subjects from 317 patients with chronic hepatitis B (CHB). HBV genotypes were determined by PCR-microcosmic nucleic acid cross-ELISA. YMDD mutations were detected by microcosmic nucleic acid cross-nucleic acid quantitative determination. HBV DNA was detected by fluorescence ratio PCR *** was given to 81 cases and its curative effect was observed by measuring ALT, HBV DNA load, HBeAg, and HBeAg/HBeAb conversion ***: HBV genotypes B, C, D, and non-classified genotypes were found in Guangxi Zhuang *** for 25.6%, 47.4%, 58.3%, and 16.0%,respectively. Seventy-four cases were CD-, CB-, BD-mixed genotypes (47.7%). Forty-six (29.5%) cases had YMDD mutations. Genotype B was mostly found in mild and moderate CHB patients. Genotypes C, D and mixed genotype mostly occurred in severe CHB *** D and CD HBV-infected patients had higher ALT and HBV DNA than patients with other types of HBV infection. There was no significant difference among the genotypes in YMDD mutations, clinical types, ALT and HBV DNA level. Non-classified types geno had a significantly lower positive rate of HBeAg than other genotypes (x2= 12.841, P＜0.05). There was no significant difference in ALT recovery rate, HBV DNA load, HBeAg,and HBeAg/HBeAb conversion rate, 48 wk after LAM treatment between groups of genotypes D, CD, and nonclassified ***: Genotypes B, C, and D, non-classified and mixed genotype of HBV are identified in the Guangxi Zhuang population. Variations in genotypes are associated with clinical severity and serum ALT levels, but not with YMDD mutation or HBV DNA *** effects of LAM on c

关键词： Hepatitis B virus Chronic hepatitis Genotype YMDD mutation Lamivudine Zhuang nationality

Novel mutations in the BEST1 gene cause distinct retinopathies in two Chinese families

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International Journal of Ophthalmology(English edition) 2022年第2期15卷 205-212页

作者： Zhi-Hong Zhu Xin Jin Yi-Xin Zhang Rui Wang Tong Wu Wei Liu Ze-Hua Chen Hai-Nan Xie Lan-Lan Chen Zi-Hao Liu hou-Bin huang Department of Ophthalmology Hainan Hospital of Chinese PLA General HospitalSanya 572013Hainan ProvinceChina Department of Ophthalmology Chinese PLA General HospitalBeijing 100853China Department of Ophthalmology the PLA Rocket Force Characteristic Medical CenterBeijing 100088China Department of Ophthalmology Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100700China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina

AIM:To describe the clinical heterogeneity of patients with novel mutations in ***:All the members in the two Chinese families underwent detailed clinical evaluations including best-corrected visual acuity,slit-lamp examination,applanation tonometry,and dilated fundus *** autofluorescence,fundus fluorescein angiography,spectral-domain optical coherence tomography,electrooculography,and electroretinogram were also *** DNA was extracted from venous blood for all the *** targeted next-generation sequencing of inherited retinal disease-associated genes was conducted to identify the causative ***:A novel BEST1 missense mutation c.41T>C(***14Ser) was identified in Family *** was co-segregated with the phenotype of best vitelliform macular dystrophy(BVMD) and bioinformatics analysis confirmed it was *** novel BEST1 frameshift mutation c.345_(3)46insGGCAAGGACG(***119Glyfs*116) and a novel USH2A missense mutation c.12560G>A,***4187 His were identified in family 2 with retinitis pigmentosa(RP),which might interact and lead to the phenotype of ***:Two novel mutations in the BEST1 gene in two unrelated families with distinct phenotypes and BEST1 mutation accompanied with USH2A mutation would result in RP,which could be enormously helpful in understanding the pathogenesis of the inherited retinal disease caused by a BEST1 mutation.

关键词： BEST1 gene best vitelliform macular dystrophy retinitis pigmentosa gene mutation

The Australian emergency medical system

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中华急诊医学杂志 2008年第7期17卷 771-775页

作者： Gerard Joseph FitzGerald Xiang-Yu hou huang Hang School of Public Health Queensland University of Technology QCD 4059 Australia

澳大利亚的急救医疗体系在过去的30年经历了本质性的转变，包括院前急救的发展，院内急诊科的发展，以及急诊专科医师和急救师作为新的职业的发展。这一本质性的转变在其他很多国家也同时存在，并缘于卫生体系提供高质量的急救医疗服务... 详细信息

澳大利亚的急救医疗体系在过去的30年经历了本质性的转变，包括院前急救的发展，院内急诊科的发展，以及急诊专科医师和急救师作为新的职业的发展。这一本质性的转变在其他很多国家也同时存在，并缘于卫生体系提供高质量的急救医疗服务的义务和不断增加的职业责任。本文的目的是回顾描述澳大利亚急救医疗体系的这一转变，以供正在建设现代化的急救医疗体系的其它国家，借鉴我们的经验教训，以面对他们可能会经历的挑战。澳大利亚的急救医疗体系并不完美，本文也没有希望其他国家照搬澳大利亚的体系，只是希望和读者分享我们自己发展的经验教训。

关键词：澳大利亚急救医疗体系医生医疗卫生事业