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Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication

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Virologica Sinica 2022年第1期37卷 48-59页

作者： Jie Zhu Qiuhong Miao hongyuan guo Aoxing Tang Dandan Dong Jingyu Tang Fang Wang Guangzhi Tong Guangqing Liu Shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghai200241China Laboratory of Virology Wageningen University and ResearchWageningen6708 PBthe Netherlands Institute of Veterinary Medicine Jiangsu Academy of Agricultural SciencesNanjing210014China

Rabbit hemorrhagic disease virus(RHDV)is a member of the Caliciviridae family and cannot be propagated in vitro,which has impeded the progress of investigating its replication *** of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host ***,aided by this replicon system and using two-step affinity purification,we purified the RHDV replicase and identified its associated host *** identified rabbit nucleolin(NCL)as a physical link,which mediating the interaction between other RNA-dependent RNA polymerase(Rd Rp)-related host proteins and the viral replicase Rd *** found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV replication in RK-13 cells,*** was identified to directly interact with RHDV Rd Rp,p16,and ***,NCL knockdown severely impaired the binding of Rd Rp to Rd Rp-related host ***,these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link between viral replicase and host proteins.

关键词： Rabbit hemorrhagic disease virus(RHDV) Nucleolin Virus replication RNA-dependent RNA polymerase(RdRp)

Environmental benefits and farmers' adoption of winter cover crops in the North China Plain

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Pedosphere 2024年第1期34卷 159-169页

作者： Shufang guo Yitao ZHANG Limei ZHAI Jian LIU hongyuan WANG Hongbin LIU State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Key Laboratory of Nonpoint Source Pollution ControlMinistry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Institute of Agricultural Environment and Resources Yunnan Academy of Agricultural SciencesKunming 650205China Shandong Yucheng Agro-ecosystem National Observation Research Station Key Laboratory of Ecosystem Network Observation and ModelingInstitute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China The Norwegian Institute of Bioeconomy Research(NIBIO) Akershus 1431Norway

The introduction of cover crops into monoculture systems to improve soil health has been widely adopted worldwide. However, little is known about the environmental risks and application prospects of different cover crops in spring maize(Zea mays L.) monocultures proposed in the North China Plain. A pot experiment was conducted to evaluate the effects of different winter cover crops on subsequent maize yield, soil fertility, and environmental risks of nitrogen(N) loss, and a questionnaire survey was conducted to examine factors influencing farmers' willingness to adopt cover crops in the North China Plain. Based on the same fertilization regime during the maize growing period, four winter cover crop treatments were set up, including bare fallow, hairy vetch(Vicia villosa Roth.), February orchid(Orychophragmus violaceus), and winter oilseed rape(Brassica campestris L.). The results indicated that winter cover crops significantly increased subsequent maize yield and soil organic carbon, total N, and microbial biomass carbon and N compared with the bare fallow *** incorporation of cover crops led to a negligible increase in nitrous oxide(N_(2)O) emissions and had a very limited effect on ammonia(NH_(3)) *** incorporation of February orchid and winter oilseed rape decreased nitrate leaching compared with the hairy vetch treatment in the maize growing *** N losses via N_(2)O and NH_(3) emissions and N leaching accounted for 71%–84% of the N surplus. However, yield increase and environmental benefits were not the main positive factors for farmers to accept cover crops. Financial incentive was rated by 83.9% of farmers as an “extremely important” factor, followed by other costs, when considering winter cover cropping. These results indicate that the environmental benefits depend on the type of cover crop. Maintaining high levels of soil fertility and maize yield, providing sufficient subsidies, and encouraging large-area cultivation of cover crops

关键词： cover crop N leaching NH_(3)emission N_(2)O emission spring maize willingness to accept

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特种尼龙成盐关键指标分析与控制

齐鲁石油化工 2024年第1期52卷 11-15页

作者：王文强王美淇郭洪元朴佳昕刘绍峰中国石油辽阳石化分公司辽宁辽阳111003

分析了生物基尼龙56、尼龙610成盐过程中温度与pH的变化趋势,对成盐样品进行了红外光谱(FTIR)和差示扫描量热法(DSC)分析,对尼龙56盐和尼龙610盐的性质进行了考察。结果表明:尼龙56盐和尼龙610盐遵循了一般尼龙成盐反应的温度和pH变化趋... 详细信息

分析了生物基尼龙56、尼龙610成盐过程中温度与pH的变化趋势,对成盐样品进行了红外光谱(FTIR)和差示扫描量热法(DSC)分析,对尼龙56盐和尼龙610盐的性质进行了考察。结果表明:尼龙56盐和尼龙610盐遵循了一般尼龙成盐反应的温度和pH变化趋势,红外光谱均具有归于尼龙的特征吸收峰,DSC分析得出尼龙56与尼龙610的熔点和结晶点与传统尼龙66接近,但稍有不同。

关键词：生物基尼龙成盐尼龙56盐尼龙610盐

新工科背景下以学生为中心的“食品化学” 课程考核评价体系的改革与实践

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农产品加工 2024年第6期 131-133,136页

作者：吴锁柱伊莉耿雪冉郑丹陈益胜郭红媛山西农业大学食品科学与工程学院山西晋中030801 山西农业大学农学院山西晋中030801

“食品化学”课程是食品相关专业的一门专业基础课程,在培养食品专业学生解决复杂工程问题的能力方面具有其他课程不可替代的作用。在新工科背景下,针对“食品化学”课程特点及教学中存在的问题,开展以学生为中心的课程教学考核评价体... 详细信息

“食品化学”课程是食品相关专业的一门专业基础课程,在培养食品专业学生解决复杂工程问题的能力方面具有其他课程不可替代的作用。在新工科背景下,针对“食品化学”课程特点及教学中存在的问题,开展以学生为中心的课程教学考核评价体系的改革与实践,旨在更科学、客观地检验学生学习效果,提升食品专业人才的培养质量。

关键词：新工科食品化学考核评价教学改革

Corrigendum to“Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp,nonstructural proteins p16 and p23,playing a role in virus replication”<[Virologica Sinica 37(2022)48-59]>

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Virologica Sinica 2023年第3期38卷 480-481页

Due to our negligence,the original version of this article,published online on 12 January 2022,contained a mistake in *** lane ofβ-actin in Western blotting was *** correct Fig.4 is given *** apologize for our oversight when preparing the figure and state that this does not change the scientific conclusions of the article in any way.

关键词： rabbit hemorrhagic Cor

Coffee Growing in a Tea Town

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China Today 2014年第9期63卷 32-33页

作者： PEDRO LAGO guo hongyuan

AHEAD of his visit to Belgium in late March,2014,Chinese President Xi Jinping published an article in the local newspaper Le Soir,titled"Friendship and Cooperation Bring Better Life to Both China and Europe."It recounted the story of a young Belgian who has taught Chinese farmers how to grow high-quality *** name is Wouter De Smet.

关键词：种植咖啡茶叶 AHEAD 比利时中国生活

The Economics of Chinese Art

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China Today 2011年第8期60卷 44-45页

作者： staff reporter guo hongyuan

IN mid-March 2011 the British Art Market Federation announced that China had overtaken the U.K. to become the world’s second-largest art market. Only a week later,Francebased Artprice,which maintains the world’s largest data bank of fine art auction records,disclosed in its 2010 report that China had climbed past the U.S. to become the top ranking country in terms of annual fine art revenues.

关键词：中国艺术经济学美术作品艺术家市场世界商铺

The Future of Tradition-Developing Hebei’s Craftsmanship Culture

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China Today 2012年第1期61卷 19-21页

作者： staff reporter guo hongyuan

HAVING studied for eight years, longer than most doctors spend in medical school, Zhang Wusuo is a highly proficient stonecutter. Zhang developed an early interest in traditional stone carving and at age 18, refusing to become a blacksmith like his father, set off to Quy- ang County for a stonecutting education. After two years of training, Zhang began to carve simple designs such as flowerbeds and fountain foundations. Nowadays he is accomplished and capable of creating Western-style statues, the

关键词：文化发展传统河北

Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients

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Signal Transduction and Targeted Therapy 2021年第9期6卷 2822-2830页

作者： Yanbo Wang Xiaoju Zhu Xia-Ming Jiang Jingwei guo Zheng Fu Zhen Zhou Ping Yang hongyuan guo Xu guo Gaoli Liang Ping Zeng Gengfu Xiao Jizheng Ma Xin Yin Lei-Ke Zhang Chao Yan Chen-Yu Zhang Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy Chinese Academy of Medical Sciences Research Unit of Extracellular RNAState Key Laboratory of Pharmaceutical BiotechnologyJiangsu Engineering Research Center for MicroRNA Biology and BiotechnologyNJU Advanced Institute of Life Sciences(NAILS)NJU Institute of Al Biomedicine and BiotechnologyChemistry and Biomedicine Innovation Center(ChemBIC)School of Life SciencesNanjing UniversityNanjingChina State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanHubeiChina Department of Clinical Laboratory The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingChina The MOH Key Laboratory of Geriatrics Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijingChina The Research Center of Military Exercise Science The Army Engineering University of PLANanjingJiangsuChina

Elderly people and patients with comorbidities are at higher risk of COVID-19 infection,resulting in severe complications and high ***,the underlying mechanisms are *** this study,we investigate whether miRNAs in serum exosomes can exert antiviral functions and affect the response to COVID-19 in the elderly and people with diabetes.

关键词： patients mortality infection