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Identification of fangchinoline as a broad-spectrum enterovirus inhibitor through reporter virus based high-content screening

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Virologica Sinica 2024年第2期39卷 301-308页

作者： Qiu-Yan zhang Jia-Qi Li Qi Li Yang zhang Zhe-Rui zhang Xiao-Dan Li hong-qing zhang Cheng-Lin Deng Feng-Xia Yang Yi Xu Bo zhang The Joint Center of Translational Precision Medicine Department of Infections and DiseasesGuangzhou Institute of PediatricsGuangzhou Women and Children's Medical CenterGuangzhou510623China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Science and Technology of China Department of Life Sciences and MedicineHefei230026China Hunan Normal University School of MedicineChangsha410081China

Hand,foot,and mouth disease(HFMD)is a common pediatric illness mainly caused by enteroviruses,which are important human ***,there are no available antiviral agents for the therapy of enterovirus *** this study,an excellent high-content antiviral screening system utilizing the EV-A71-eGFP reporter virus was *** this screening system,we screened a drug library containing 1042 natural compounds to identify potential EV-A71 ***(FAN),a bis-benzylisoquinoline alkaloid,exhibits potential inhibitory effects against various enteroviruses that cause HFMD,such as EV-A71,CV-A10,CV-B3 and *** investigations revealed that FAN targets the early stage of the enterovirus life *** the selection of FAN-resistant EV-A71 viruses,we demonstrated that the VP1 protein could be a potential target of FAN,as two mutations in VP1(E145G and V258I)resulted in viral resistance to *** research suggests that FAN is an efficient inhibitor of EV-A71 and has the potential to be a broad-spectrum antiviral drug against human enteroviruses.

关键词： Hand,foot,and mouth disease(HFMD) Enterovirus EV-A71 High-content screening Fangchinoline(FAN)

Effect of Jianpi Shengxue Tablet on Iron Metabolism and Nutritional Status in Patients with Renal Anemia:A Prospective,Randomized,Open,Parallel Controlled and Multicenter Clinical Study

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Current Medical Science 2024年第3期44卷 603-610页

作者： Juan YANG Meng-yuan LIANG Yu LI Hao zhang Wei LI Jing LV Li-ping DONG Jing-song JIN Cheng YANG qing-hong zhang Li LONG Rong ZOU Yi GAO Dan SONG Chun-qin PAN Ying YAO Xiao-lin ZHAN Xiao-hui WANG Department of Nephrology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Nephrology The First People’s Hospital of GuiyangGuiyang550000China Department of Nephrology The Third Xiangya HospitalCentral South UniversityChangsha410000China Department of Nephrology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an710061China Department of Nephrology Wuhan No. 4 HospitalWuhan430033China Department of Nephrology Hubei Provincial Hospital of Traditional Chinese MedicineWuhan430060China Department of Nephrology Central Theater General Hospital of PLAWuhan430064China Department of Nephrology Taihe HospitalShiyan442000China Department of Nephrology Third People’s Hospital of Hubei ProvinceWuhan430033China Department of Nephrology Hubei Province Integrated Traditional Chinese and Western Medicine Hospital of Hubei provinceWuhan430024China Department of Nephrology Xi’an No.3 HospitalAffiliated Hospital of Northwest UniversityXi’an710000China Department of Nephrology Shenzhen Guangming District People’s HospitalShenzhen518000China Department of Nephrology Xiantao First People’s HospitalXiantao433000China Department of Nutrition Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Nephrology Huangshi Central HospitalHuangshi435000China Department of Nephrology The Fifth Hospital of WuhanWuhan430050China

Objective This study aimed to analyze the clinical efficacy of the Jianpi Shengxue tablet for treating renal *** A total of 200 patients with renal anemia from December 2020 to December 2022 were enrolled and randomly divided into two *** in the control group were treated with polysaccharide-iron complex,and those in the experimental group were administered Jianpi Shengxue *** 8 weeks of continuous treatment,the therapeutic outcomes regarding anemia were compared between the two *** After treatment,the red blood cell(RBC)count,hematocrit(HCT),reticulocyte percentage(RET),ferritin(SF),serum iron(SI),transferrin saturation(TSAT),and serum albumin(ALB)all increased(P<0.01),and the clinical symptom score and total iron binding capacity decreased(P<0.01)in the experimental ***,the improvements in RBC,HCT,RET,SF,SI,TAST,ALB,and clinical symptoms(fatigue,anorexia,dull skin complexion,numbness of hands and feet)in the experimental group were significantly greater than those in the control group(P<0.05).The total effective rate for treating renal anemia was significantly higher in the experimental group than in the control group(P<0.01).Conclusion The Jianpi Shengxue tablet demonstrates efficacy in treating renal anemia,leading to significant improvements in the laboratory examination results and clinical symptoms of patients with renal anemia.

关键词： Jianpi Shengxue tablet renal anemia randomized open parallel control multicenter clinical study

Global Parametric Sufficient Efficiency Conditions for Semiinfinite Multiobjective Fractional Programming Problems Containing Generalized (α, η, ρ)-V-Invex Functions

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Acta Mathematicae Applicatae Sinica 2013年第1期29卷 63-78页

作者： G.J. Zalmai qing-hong zhang Department of Mathematics and Computer Science Northern Michigan University

Abstract In this paper, we discuss numerous sets of global parametric sufficient efficiency conditions under various generalized （a,n, p）-V-invexity assumptions for a semiinfinite multiobjective fractional programmi... 详细信息

关键词： Semiinfinite programming multiobjective fractional programming generalized invex functions,infinitely many equality and inequality constraints parametric sufficient efficiency conditions.

Parametric Duality Models for Semiinfinite Multiobjective Fractional Programming Problems Containing Generalized (α, η, ρ)-V-Invex Functions

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Acta Mathematicae Applicatae Sinica 2013年第2期29卷 225-240页

作者： G.J. ZALMAI qing-hong zhang Department of Mathematics and Computer Science Northern Michigan University

In this paper, we present several parametric duality results under various generalized （a,v,p）-V- invexity assumptions for a semiinfinite multiobjective fractional programming problem.

关键词： Semiinfinite programming multiobjective fractional programming generalized invex functions infinitely many equality and inequality constraints parametric duality models duality theorems

Determination of PDCD5 in Peripheral Blood Serum of Cancer Patients

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Chinese Journal of Cancer Research 2011年第3期23卷 224-228页

作者： Yue Wang Guo-hong Wang qing-yun zhang Key Laboratory of Carcinogenesis and Translational Research （Ministry of Education） Department of Clinical LaboratoryPeking University School of OncologyBeijing Cancer Hospital ＆ InstituteBeijing 100142China

Objective：Programmed cell death 5 （PDCD5） is an apoptosis related gene and plays an important role in the pathogenesis and development of *** PDCD5 is present in peripheral blood serum has not been *** aim of this study is to determine the contents of PDCD5 protein in peripheral blood serum of cancer patients,as well as normal ***：ELISA was used to detect the serum PDCD5 concentrations in 100 normal persons,83 patients with breast cancer,74 patients with gastrointestinal tract cancer and 41 patients with lung *** results were statistically analyzed and ***：PDCD5 could be detected in peripheral blood serum in both normal subjects and cancer *** serum PDCD5 contents in normal persons ranged from 3.8 to 6.1 ng/ml with a median of 4.70±0.68 ng/*** cancer patients the PDCD5 levels were 4.59±0.90,4.79±1.14 and 10.43±22.34 ng/ml for breast cancer,gastrointestinal cancer and lung cancer patients *** was no statistically significant difference between the serum PDCD5 concentrations of normal persons and cancer ***：PDCD5 is present in peripheral *** PDCD5 levels in cancer patients are not statistically different from that of normal persons,though decreased expression of PDCD5 in malignant tissues has been found.

关键词： PDCD5 Apoptosis Cancer patients ELISA

Effect of Bcl-2 and caspase-3 on calcium distribution in apoptosis of HL-60 cells

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Cell Research 2000年第3期10卷 213-220页

作者： zhang MIN, hong qing zhang, SHAO BAI XUE (Department of Biology, Beijing Normal University, Beijing 100875, China) DepartmentofBiology BeijingNormalUniversityChina.

Apoptosis manifests in two major execution programs downstream of the death signal: the caspase pathway and organelle dysfunction. An important antiapoptosis factor, Bcl-2 protein, contributes in caspase pathway of apoptosis. Calcium, an important intracellular signal element in cells, is also observed to have changes during apoptosis, which maybe affected by Bcl-2 protein. We have previously reported that in Harringtonine (HT) induced apoptosis of HL-60 cells, there’s a change of intracellular calcium distribution, moving from cytoplast especially Golgi’s apparatus to nucleus and accumulating there with the highest concentration. We report here that caspase-3 becomes activated in HT-induced apoptosis of HL-60 cells, which can be inhibited by overexpression of Bcl-2 protein. No sign of apoptosis or intracellular calcium movement from Golgi’s apparatus to nucleus in HL-60 cells overexpressing Bcl-2 or treated with Ac-DEVD-CHO, a specific inhibitor of caspase-3. The results indicate that activated caspase-3 can promote the movement of intracellular calcium from Golgi’s apparatus to nucleus, and the process is inhibited by Ac-DEVD-CHO (inhibitor of caspase-3), and that Bcl-2 can inhibit the movement and accumulation of intracellular calcium in nucleus through its inhibition on caspase3. Calcium relocalization in apoptosis seems to be irreversible, which is different from the intracellular calcium changes caused by growth factor.

关键词： Apoptosis calcium caspase-3 bcl-2 laser scanning confocal microscopy (LSCM).

Intranasal delivery of replicating mRNA encoding neutralizing antibody against SARS-CoV-2 infection in mice

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Signal Transduction and Targeted Therapy 2021年第11期6卷 3333-3340页

作者： Jia-Qi Li Zhe-Rui zhang hong-qing zhang Ya-Nan zhang Xiang-Yue Zeng Qiu-Yan zhang Cheng-Lin Deng Xiao-Dan Li Bo zhang Han-qing Ye Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanChina University of Chinese Academy of Sciences 100049BeijingChina School of Medicine Hunan Normal University410081ChangshaChina

The lung is the prophylaxis target against SARS-CoV-2 infection,and neutralizing antibodies are a leading class of biological products against various infectious viral *** this study,we develop a safe and cost-effective platform to express neutralizing antibody in the lung with replicating mRNA basing on alphavirus replicon particle(VRP)delivery system,to prevent SARS-CoV-2 ***,a modified VEEV replicon with two subgenomic(sg)promoters was engineered to translate the light and heavy chains of antibody simultaneously,for expression and assembly of neutralizing anti-SARS-CoV-2 antibody ***,the feasibility and protective efficacy of replicating mRNA against SARS-CoV-2 infection were demonstrated through both in vitro and in vivo *** lung target delivery with the help of VRP system resulted in efficiently block SARS-CoV-2 infection with reducing viral titer and less tissue damage in the lung of ***,our data suggests that expressing neutralizing antibodies in the lungs with the help of self-replicating mRNA could potentially be a promising prophylaxis approach against SARS-CoV-2 infection.

关键词： infection lung protective

Dysfunction of neuroendocrine system in sepsis and implication of hormone therapy

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Journal of Geriatric Cardiology 2009年第4期6卷 249-254页

作者： qing-hong zhang Yong-Ming Yao Zhi-Yong Sheng Department of Microbiology and Immunology Burns Institute First Hospital Affiliated to Chinese PLA General Hospital Beijing100048 China

Sepsis and related syndromes are the major cause of multiple organ failure and death in patients with critical illnesses. Neuroendocrine dysfunction has long been thought to be an important event in sepsis. In clinic, optimal management of the hormones could alleviate severe complications in sepsis. In this article, we review the dysfunction ofneuroendocrine system as well as autonomic nervous system in sepsis, and summarize the respective therapy strategies.

关键词： sepsis neuroendocrine autonomic nervous system immunity

Simultaneous bilateral fractures of the femoral neck caused by high energy： A case report and literature review

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Chinese Journal of Traumatology 2015年第5期18卷 304-306页

作者： You-Shui Gao Zhen-hong Zhu Chang-qing zhang Department of Orthopedic Surgery Shanghai flao Tong University Affiliated Sixth People's Hospital 600 Yishan Road Shanghai 200233 China

Simultaneous bilateral fractures of the femoral neck are rare injuries, which are reportedly induced by low-speed energy with predisposing factors including systemic diseases, medications and eclamptic seizures. Those caused by high energy are even rarer. High energy-induced bilateral fractures of the femoral neck conceive of high incidence of mortality and present great challenges in the early man- agement. We report one case of a 52-year-old man with simultaneous bilateral fractures of the femoral neck which resulted from a motor pedestrian accident. One-stage closed reduction and internal fixation was done following the emergent resuscitation and neurosurgical management for concomitant brain injuries. The fractures united. There was no pain in the hips, and they had a normal range of motion. The treatment protocol, mechanism of the injury and possible postoperative complications were discussed to expand a comprehensive understanding about these infrequent types of fractures.

关键词： Femoral neck fractures Clinical protocol Postoperative complications