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Role of c-Jun NH_(2)-terminal kinase-mediated mitogen-activated protein kinase pathway in response to pesticides in Apis cerana cerana

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Insect Science 2023年第1期30卷 47-64页

作者： Xiao-Jing Niu Li-Jun wang Hui Meng hong-fang wang Bao-Hua Xu Chen wang State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandong ProvinceChina College of Animal Science and Technology Shandong Agricultural UniversityTaianShandong ProvinceChina

The mitogen-activated protein kinase(MAPK)cascade pathway plays an important role in regulating stress *** function of the c-Jun NH_(2)-terminal kinase(JNK),a component of the MAPK cascade pathway,in Apis cerana cerana(Acc)remains ***,JNK was isolated and identified from *** analyses revealed there is a typical serine/threonine protein kinase catalytic domain in the AccJNK *** expression profile analysis showed that AccJNK was significantly induced by pesticide *** further explore the functional mechanisms of AccJNK,a yeast 2-hybrid screen was performed,activator protein-1(AP-1)was screened as the interaction partner of AccJNK,and the interaction relationship was further verified by pull-down *** real-time polymerase chain reaction showed the expression pattern of AccAP-I was similar to that of *** a knockdown of AccJNK or AccAP-I by RNA interference,the survival rate of Acc after pesticide treatments ***,the expression levels of antioxidant-related genes and the activities of antioxidant enzymes increased,suggesting that the knockdown of AccJNK or AccAP-I increased the antioxidant capacity of *** study revealed that the JNK-mediated MAPK pathway responds to pesticide stress by altering the antioxidant capacity of Acc.

关键词： Apis cerana cerana MAPK cascade oxidative stress pesticide stress RNA interference

Methionine as a methyl donor regulates caste differentiation in the European honey bee(Apis mellifera)

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Insect Science 2021年第3期28卷 746-756页

作者： Wen-Feng Chen Ying wang Wei-Xing Zhang Zhen-Guo Liu Bao-Hua Xu hong-fang wang College of Animal Science and Technology Shandong Agricultural UniversityTaianShandong ProvinceChina

Nutrition contributes to honey bee caste differentiation,but the role of individual nutrients is still *** essential amino acid contents,except that of methionine(Met),are greater in royal jelly than worker ***〜3.5 i the Met content in the latter was slightly greater than in the *** is the major raw material used in the synthesis of S-adenosyl-L-methionine,an active methyl donor for DNA methylation,which is an epigenetic driver of caste ***,we tested whether Met regulates caste differentiation in honey bees by determining its effects on the caste development of bees receiving four diets:the basic,basic+0.2%Met,basic+0.2%Met+20 mg/kg 5-azacytidine,and basic+20 mg/kg *** presence of Met decreased the adult bee body length and the numbers of ovarioles,indicating that Met may direct the development of female larvae toward worker *** upregulated expression of SAMS,Dnmtl,and Dnmt3 caused by Met exposure in 4-d-old larvae indicated that the worker-inductive effects of Met may occur through the promotion of DNA *** investigated the co-effects of Met and glucose on bee development,and found that the effects of an increased glucose level on the number of ovarioles and body length did not strengthen the worker-inductive effects caused by *** results contribute to caste development theory and suggest that Met-as a methyl donor一plays a regulatory,but not decisive,role in caste differentiation.

关键词： Apis mellifera body length caste differentiation methionine methyl donor ovarioles

Effect of thermal deformation on microstructure and properties of TC18 titanium alloy produced by laser additive manufacturing

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Journal of Iron and Steel Research International 2020年第12期27卷 1476-1484页

作者： Xiao-dong Li Chang-yue Qiu Yu-ting Liu hong-fang wang Dong-dong Zheng Yan-yan Zhu Shu-quan Zhang National Engineering Laboratory of Additive Manufacturing for Large Metallic Components and Engineering ResearchCenter Beijing 100191China Ministry of Education on Laser Direct Manufacturing for Large Metallic Components Beijing 100191China Beijing Engineering Technological Research Center on Laser Direct Manufacturing for Large Critical Metallic Component Beijing 100191China School of Materials Science and Engineering Beihang UniversityBeijing 100191China

Grain boundary of α phase damaged ductility of laser melting-deposited TC18 titanium alloy and grain boundary of α phases were difficult to break by nominal heat treatment. An extra thermal deformation was introduced to break the grain boundary of α phase with the improved mechanical property of TC18 titanium alloy fabricated by laser melting deposition *** indicated that after thermal deformation, β grains in alloy seriously elongated. When sample was deformed at temperatures from 750 to 850 ℃, α phase exhibited both rod and irregular morphologies with discontinuous distribution at grain boundary, and the subsequent heat treatment would lead to spheroidization of the α phase. However, after deformation at 900 ℃, α phase transferred into β phase and the subsequent heat treatment would make continuous grain boundary of α phase reappear. The suitable hot deformation can effectively break the continuous grain boundary in laser melting-deposited TC18 alloy with respected improved ductility.

关键词： Laser additive manufacturing Nearβtitanium alloy Thermal deformation Heat treatment Microstructural characteristics

Effects of metoclopramide on gastrointestinal myoelectric activity in rats

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World Journal of Gastroenterology 1997年第3期3卷 41-42页

作者： QIN Xiao Min 1, LI hong fang 1 and wang Long De 2

AIM To investigate the differences of metoclopramide (MCP) action on myoelectric activity of antrum and small intestine. METHODS Ten healthy male Wistar rats, weighing 250g～350g, were anesthetized with ketamine hydrochloride (100mg/kg, intramuscularly). Four pairs of bipolar stainless steel electrodes 3mm apart were implanted on serosal surface of antrum and 1, 10 and 20cm distal to pylorus respectively. Five to 10 days after operation, the gastrointestinal myoelectric activity of fasted rats after intramuscular injection of 2 5, 6 and 12mg/kg MCP respectively ws recorded with a 8 channel EEG machine, and was quantitatively compared with the myoelectric activity after saline injection. RESULTS In fasted rats, 2 5mg/kg MCP increased the amplitude of spike activity (402 0μV±138 4μV, vs 345 0μV±163 4μV, P <0 05) and the percentage of the slow wave containing spike bursts (60 4%±22 0% vs 47 4%±22 5%, P <0 01) of small intestine (1cm distal to pylorus), but did not affect the myoelectric activity of antrum. Six and 12mg/kg MCP increased the amplitude of both the slow wave (332 8μV±200 1μV vs 191 2μV±143 9μV, P <0 01; 330 0μV±197 1μV vs 191 2μV±143 9μV, P <0 05) and the spike activity (180 5μV±69 7μV vs 121 8μV±63 3μV, P <0 05; 174 5μV±71 7μV vs 123 8μV±63 3μV, P <0 05) of antrum, while in small intestine (1cm distal to pylorus) only that of the amplitude of spike activity (407 3μV±179 0μV vs 345 0μV±163 4μV, P <0 05; 456 0μV±145 4μV vs 345 0μV±163 4μV, P <0 05) and the percentage of the slow wave containing spike bursts (61 7%±26 5% vs 47 4%±22 5%, P <0 01; 59 1%±17 3% vs 47 4%±22 5%, P <0 01) was increased and with latent period significantly prolonged (2 5min±0 35min vs 0 77min±0 18min, P <0 01). CONCLUSION Different mechanisms may be involved in increasing the myoelectric activity of antrum and small intestine after MCP administ

关键词： metoclopramide \ stomach \ intestine myoelectric activity

Short-term effect of cardiac resynchronization therapy in patients with ischaemic or nonischaemic cardiomyopathy

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Chinese Medical Journal 2006年第18期119卷 1507-1510页

作者： HUA Wei NIU hong-xia wang fang-zheng ZHANG Shu CHEN Ke-ping CHEN Xin Department of Clinical Electrophysiology Fuwai Hospital and Cardiovascular Institute Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100037 China

Background Patients with heart failure were candidates for cardiac resynchronization therapy （CRT） regardless of underlying aetiology. This study observed the effect of CRT in patients with ischaemic or nonischaemic cardiomyopathy. Methods One hundred and forty-two patients with refractory chronic heart failure and left bundle branch block received cardiac resynchronization therapy, 91 men and 51 women, average age 60 years. Left ventricular ejection fraction （LVEF） was severely depressed （mean 29%）, left ventricular end diastolic diameter （LVEDD） enlarged （mean 72 mm） and QRS width was lengthened （mean 147 ms）. Ninety-eight had nonischaemic cardiomyopathy and 44 had ischaemic cardiomyopathy. Results After cardiac resynchronization therapy, the heart function was significantly improved. The mean LVEF increased from 29% to 36% after pacing. In patients with nonischaemic cardiomyopathy, the LVEF was improved from 28% to 37%, and in patients with ischaemic cardiomyopathy, the LVEF was improved from 30% to 36%. No significant difference of the improvement was found between the two groups （P〉0.05）. Conclusions Cardiac resynchronization therapy could significantly improve cardiac function in patients with chronic heart failure regardless of the underlying heart disease.

关键词： cardiac resynchronization therapy cardiomyopathy, ischaemic nonischaemic

Preventive Countermeasures for "Immiserizing Growth in Expanding Economies" in China's Rural Areas in the New Era

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Asian Agricultural Research 2011年第9期3卷 1-5页

作者： PANG Yong-hong,wang fang-fang,LIU You-bin School of Trade and Administration,Chongqing University,Chongqing 400030,China

On the basis of defining the concept of "immiserizing growth in expanding economies",through analysing the status quo of economy in China's rural areas in the new era,this paper aims to find out the factors responsible for economic weakness of China's rural *** analyses the impact of market consuming economy on growth economy,and points out the phenomenon of "immiserizing growth in expanding economies" which may be triggered by China's rapid rural economic growth as follows:first,the income disparity between urban and rural residents increases ceaselessly,the commodity prices continuously soar,and the rural residents' market consuming capacity is short;second,the wealth concentrates highly and the majority of farmers' income is *** we put forward the countermeasures and proposals for preventing "immiserizing growth in expanding economies" in rural areas as follows:we should reform household registration system so that the rural residents have the right to migrate freely,and farmers' status is promoted;we should coordinate urban-rural development,strengthen agriculture and rural infrastructure building and achieve impartial allocation of public resources;we should carry out redistribution on income scientifically and effectively,consolidate rural social security system,bridge urban-rural income gap,transform the comparative advantage into competitive advantage,so as to circumvent "immiserizing growth in expanding economies".

关键词： Immiserizing Growth in Expanding Economies Social

Study of the Influential Factors and Pathogenesis in Skin Aging

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Study of the Influential Factors and Pathogenesis in Skin Ag...

Effects of tetrandrine on calcium and potassium currents in isolated rat hepatocytes

中华医学会第十二次全国皮肤性病学术会议

作者： fang hong, wang Yi-na, Zhu Weifang The First affiliated hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Purpose To investigate the influential factors and pathological changes involved in skin aging of Hangzhou (southeast China) people. Methods Questionnaire and researcher’s assessment on skin wrinkles, Fitzpatrick type, elasticity and concomitant cutaneous diseases were applied to analyze the influential factors of the skin aging in Hangzhou people of different age. Routine histopathology, Aldehyde fuchsin staining for elastic fibers and immunochemical detection for cy

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World Journal of Gastroenterology 2003年第1期9卷 134-136页

作者： hong-Yi Zhou fang wang Lan Cheng Li-Ying Fu Ji Zhou Wei-Xing Yao Department of Pharmmacology,Tongji medical college of Huazhong university of science and technology,Wuhan 430030,Hubei Province,China Department of Pharmacology Tongji medical college of Huazhong university of science and technology Wuhan 430030 Hubei Province China. zhouhy518@***

AIM: To study the effects of tetrandrine (Tet) on calciumrelease-activated calcium current (ICRAC), delayed rectifierpotassium current (IK), and inward rectifier potassiumcurrents (IK1) in isolated rat ***: Hepatocytes of rat were isolated by usingperfusion method. Whole cell patch-clamp techniques wereused in our ***: The peak *** ICRAC was -508±115 pA(n＝15), its reversal potential of ICRAC was about 0 mV. At thepotential of -100 mV, Tet inhibited the peak amplitude ofICRAC from -521±95 pA to -338±85 pA (P＜0.01 vs control,n＝5), with the inhibitory rate of 35 % at 10 μmol/L andfrom -504±87 pA to -247±82 pA (P＜0.01 vscontrol, n＝5),with the inhibitory rate of 49 % at 100 μmol/L, withoutaffecting its reversal potential. The amplitude of ICRAC wasdependent on extracellular Ca2+ concentration. The peakamplitude of ICRAC was -205±105 pA (n＝3) in tyrode's solutionwith Ca2+ 1.8 mmol/L (P＜0.01 vs the peak amplitude ofICRAC in external solution with Ca2+ 10 mmol/L). Tet at theconcentration of 10 and 100 μmol/L did not markedly changethe peak amplitude of delayed rectifier potassium currentand inward rectifier potassium current (P＞0.05 vs control).CONCLUSION: Tet protects hepatocytes by inhibiting ICRAC,which is not related to I K and IK1.

关键词：粉防已碱钾离子电流钙离子电流肝实质细胞药理作用

基于三明医改的我国健康保障领域中的政府责任分析

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中华医院管理杂志 2017年第4期33卷 252-254页

作者：陶思羽乐虹黄阿红方鹏骞王方华中科技大学同济医学院医药卫生管理学院武汉430030 华中科技大学同济医学院医药卫生管理学院健康政策与管理研究院解放军武汉总医院医务处

基于三明市医药卫生体制改革中政府有力主导发挥的关键作用，探讨了我国政府基于国家战略推进、实现公众利益和弥补市场必然要求应在健康保障中承担相应的责任，梳理了政府为实现公民健康保障应承担的保障、领导、监督和管理责任，针对... 详细信息

基于三明市医药卫生体制改革中政府有力主导发挥的关键作用，探讨了我国政府基于国家战略推进、实现公众利益和弥补市场必然要求应在健康保障中承担相应的责任，梳理了政府为实现公民健康保障应承担的保障、领导、监督和管理责任，针对当前政府尚存在的责任缺位和错位的问题提出了政策建议，包括大力推进健康保障法制建设、加大政府对健康保障的投入、建立健全综合监管制度、组建综合健康责任主体。

关键词：三明市医药卫生体制改革健康保障政府责任