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基于蛋白组学探讨灵芝酸X治疗肝母细胞瘤的作用机制

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中国中药杂志 2024年第15期49卷 4158-4166页

作者：叶婷高航葛洋沈瑞余鸿雁陈峰远宋航安徽中医药大学研究生院安徽合肥230012 安徽中医药大学中西医结合学院安徽合肥230012 蚌埠医学院安徽省转化性肿瘤研究重点实验室安徽蚌埠233030

基于蛋白组学探讨灵芝酸X(ganoderic acid X,GAX)对人肝母细胞瘤HepG2、HuH6细胞模型和非肥胖型糖尿病/重症联合免疫缺陷(nonobese diabetic-severe combined immune deficient,NOD-SCID)小鼠皮下移植瘤模型的作用机制,为灵芝酸X的临床应用提供依据。采用CCK-8法检测灵芝酸X对HepG2、HuH6细胞活力的影响;EdU实验检测灵芝酸X对细胞增殖的影响;划痕实验检测灵芝酸X对细胞迁移能力的影响;Hoechst 33258染色检测灵芝酸X对细胞凋亡的影响;建立NOD-SCID小鼠皮下移植瘤模型,分析对照组及灵芝酸X低、中、高剂量组(5、10、20 mg·kg^(-1))肿瘤体积和质量;苏木素-伊红(HE)染色评估灵芝酸X的药物毒性。此外,收集HepG2细胞对照组和灵芝酸X高剂量组的细胞,分别进行laber-free蛋白组学分析,筛选差异蛋白并富集相关信号通路,CYTO-ID?染色检测细胞自噬情况,Western blot实验检测相关蛋白的表达量。体外结果显示,灵芝酸X呈剂量依赖性抑制HepG2、HuH6细胞的增殖、迁移、诱导凋亡;体内研究显示灵芝酸X显著抑制肿瘤体积和质量,且对小鼠主要脏器(心、肝、脾、肺、肾)无明显损害;laber-free蛋白组学结果显示灵芝酸X在肝母细胞瘤治疗过程中参与多种信号通路,其中自噬途径高度富集。CYTO-ID?染色及Western blot结果显示灵芝酸X诱导细胞自噬并上调苄氯素1(Beclin-1)、自噬相关基因5(ATG5)、微管相关蛋白1A/1B-轻链3(LC3)-Ⅱ蛋白表达量,下调螯合体1(p62)蛋白表达量。该研究表明,灵芝酸X通过诱导自噬进而抑制肝母细胞瘤细胞的增殖、迁移和诱导凋亡,并显著抑制肿瘤生长,是一种有希望的癌症辅助治疗药物。

关键词：灵芝酸X 肝母细胞瘤蛋白组学自噬

Plant phenolics mediated bottom-up effects of elevated CO2 on Acyrthosiphon pisum and its parasitoid Aphidius avenae

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Insect Science 2020年第1期27卷 170-184页

作者： hong-yu yan hong-Gang Guo yu-Cheng Sun Feng Ge State Key Laboratory of Integrated Management of Pest and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina

Elevated concentrations of atmospheric CO2 can alter plant secondary metabolites,which play important roles in the interactions among plants,herbivorous insects and natural ***,few studies have examined the cascading effects of host plant secondary metabolites on tri-trophic interactions under elevated CO2(eCO2).In this study,we determined the effects of eCO2 on the growth and foliar phenolics of Medicago truncatula and the cascading effects on two color genotypes oiAcyrthosiphon pisum(pink ***)and their parasitoid Aphidius avenae in the field open-top *** results showed that eCO2 increased photosynthetic rate,nodule number,yield and the total phenolic content of ***2 had contrasting effects on two genotypes of ***;the green genotype demonstrated increased population abundance,fecundity,growth and feeding efficiency,while the pink genotype showed decreased fitness and these were closely associated with the foliar genstein ***,eCO2 decreased the parasitic rate of *** independent of aphid ***2 prolonged the emergence time and reduced the emergence rate and percentage of females when associated with the green genotype,but little difference,except for increased percentage of females,was observed in *** under eCO2 when associated with the pink genotype,indicating that parasitoids can perceive and discriminate the qualities of aphid *** concluded that eCO2 altered plant phenolics and thus the performance of aphids and *** results indicate that plant phenolics vary by different abiotic and biotic stimuli and could potentially deliver the cascading effects of eCO2 to the higher trophic *** results also suggest that the green genotype is expected to perform better in future eCO2 because of decreased plant resistance after its infestation and decreased parasitic rate.

关键词： Acyrthosiphon pisum:Aphidius avenae elevated CO2 Medicago truncatula' phenolics tri-trophic level

基于MaxEnt模型的祁连山南麓门源草原毛虫分布区预测

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昆虫学报 2024年第6期67卷 827-838页

作者：刘玉英严林于红妍牟丹赵啟军林扎西尖措高太侦谢久祥青海大学农牧学院西宁810016 祁连山国家公园青海服务保障中心西宁810000 天峻县林业和草原站天峻817200

【目的】门源草原毛虫Gynaephora menyuanensis是为害青藏高原高寒草甸的主要害虫,严重妨碍草地生态和畜牧业发展,了解其在祁连山南麓的实际分布区和潜在适生区对门源草原毛虫的监测和防治以及草地生态的保护具有重要意义。【方法】本... 详细信息

【目的】门源草原毛虫Gynaephora menyuanensis是为害青藏高原高寒草甸的主要害虫,严重妨碍草地生态和畜牧业发展,了解其在祁连山南麓的实际分布区和潜在适生区对门源草原毛虫的监测和防治以及草地生态的保护具有重要意义。【方法】本研究在对祁连山南麓门源草原毛虫的分布进行大量实地调查(147个位点)的基础上,采用专业统计分析软件IBM SPSS 22.0筛选影响门源草原毛虫分布的环境因子,应用生态位模型MaxEnt 3.4.4和地理信息系统软件ArcGIS 10.8预测祁连山南麓门源草原毛虫2021-2040年的适生区范围。【结果】影响门源草原毛虫分布的主要环境因子为9月平均降水量、 9月平均太阳辐射等;2021-2040年门源草原毛虫在祁连山南麓的适生区变化趋势为高适生区和非适生区的面积呈减少趋势,中适生区和低适生区面积呈增加趋势;检测模型的受试者工作特征(receiver operating characteristic, ROC)曲线下的面积(area under ROC, AUC)值为0.924,模拟结果准确性较好。【结论】本研究结果为阐明门源草原毛虫的地理分布规律及其监测防治提供了理论参考。全球气候变化为门源草原毛虫的栖息地扩张提供了适宜的条件,建议根据门源草原毛虫的潜在适生等级,构建“分级应对”的监测和早期预警体系与防控模式,以应对害虫为害。

关键词：门源草原毛虫青藏高原草地保护草地害虫潜在分布区分布规律

基于BP神经网络的乒乓球优秀女子单打比赛结果预测模型构建及仿真应用

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哈尔滨体育学院学报 2024年第2期42卷 89-96页

作者：修霆喆于红妍黄雯妍上海交通大学上海200240

为构建优秀女子运动员技战术表现与比赛获胜的理论模型,合理安排技战术训练及比赛策略提供参考借鉴。运用录像观察法、数理统计法及BP神经网络构建乒乓球优秀女子单打比赛结果预测模型。在此基础上,利用预测模型仿真分析新型塑料球时代... 详细信息

为构建优秀女子运动员技战术表现与比赛获胜的理论模型,合理安排技战术训练及比赛策略提供参考借鉴。运用录像观察法、数理统计法及BP神经网络构建乒乓球优秀女子单打比赛结果预测模型。在此基础上,利用预测模型仿真分析新型塑料球时代优秀女子乒乓球运动员在不同水平技战术组合下的比赛获胜模式。本文构建的预测模型R为0.978,R~2为0.956,平均绝对误差为0.0085,模型精度达到98.4%;仿真分析1 024种技战术段组合结果可知,568种组合的预测结果为获胜,456种组合的预测结果为失败。结论:基于BP神经网络构建的乒乓球优秀女子单打比赛结果预测模型拟合效果佳,个案实证预测效果较好,具有较高的预测性能;新型塑料球时代优秀女子运动员在单打比赛中,各技战术段之间的补偿效应因技战术段和等级而有所不同,评估总分17分为女子单打比赛胜负的分界点,不同水平技战术段组合的比赛评估总分大于17分即可取得比赛胜利,低于17分则会落败。

关键词：乒乓球女子单打 BP神经网络预测模型

Online upgrading of organic vapors from the fast pyrolysis of biomass

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燃料化学学报 2008年第6期36卷 666-671页

作者： LI hong-yu yan Yong-jie REN Zheng-wei Research Center for Biomass Energy State Key Laboratory of Bioreactor Engineering East China University of Scince and Technology Shanghai 200237

The online upgrading process that combined the fast pyrolysis of biomass and catalytic cracking of bio-oil was developed to produce a high quality liquid product from the *** installation consisted of a fluidized bed reactor for pyrolysis and a packed bed reactor for *** proper pyrolysis processing conditions with a temperature of 500℃ and a flow rate of 4m3·h-1 were determined in *** such conditions,the effects of temperature and weight hourly space velocity(WHSV) on both the liquid yields and the oil qualities of the online catalytic cracking process were *** results showed that such a combined process had the superiority of increasing the liquid yield and improving the product quality over the separate ***,when the temperature was 500℃,with a WHSV of 3h-1,the liquid yield reached the maximum and the oxygenic compounds also decreased obviously.

关键词：有机水蒸气高温分解反应生物量化学分析

Highly sensitive fluorescence quantification of irinotecan in biological fluids with the aid of second-order advantage

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Chinese Chemical Letters 2010年第12期21卷 1482-1486页

作者： Hai yan Fu Hai Long Wu Jin Fang Nie Yong Jie yu hong yan Zou Ru Qin yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China

A rapid,green and highly sensitive excitation-emission matrix（EEM） fluorescence method was proposed for analysis of irinotecan（CPT-11） in biological fluids including human plasma and urine samples of uncalibrated interferences with the aid of second-order *** to the serious spectral overlapping from biological matrices,the parallel factor analysis（PARAFAC） and the alternating normalization-weighted error（ANWE） have been recommended to perform directly calibration and overcome the problem which makes the traditional fluorospectrophotometer in *** results can be ***, performance of the proposed method was evaluated based on figures of merit and some statistical *** accuracy of both algorithms was validated by the elliptical joint confidence region（EJCR） *** precision and repeatability were also investigated by the relative standard deviations（RSDs） of intra-day and inter-day.

关键词： Irinotecan Biological fluids Excitation-emission matrix fluorescence PARAFAC ANWE

Synthesis of novel redox calix[4]arene derivative with 1,3-substituted large conjugated ferrocene bridge

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Chinese Chemical Letters 2009年第9期20卷 1039-1041页

作者： Bi Qiong hong Fa Fu yang hong yu Guo yan Hua Wang Xian Feng Chai College of Chemistry and Materials Fufian Normal University Fuzhou 350007 China

A novel redox calix[4]arene derivative with 1,3-substituted large conjugated ferrocene bridge was designed and synthesized in high yield. Its structure was characterized by element analysis, IR, ESI-MS, NMR spectrum, etc. The primary study of cyclic voltammetry showed it possessed excellent reversible electrochemistry response property. 2009 Fa Fu yang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： Calixarene Ferrocene Synthesis Cyclic voltammetry

Imaging characteristics of a rare case of monostotic fibrous dysplasia of the sacrum:A case report

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World Journal of Clinical Cases 2021年第5期9卷 1111-1118页

作者： Xin-Xin Liu Xin Xin yu-hong yan Xiao-Wen Ma MRI Department Honghui HospitalXi'an Jiaotong UniversityXi'an 710054Shaanxi ProvinceChina Department of Orthopaedics Ankang Center HospitalAnkang 725000Shaanxi ProvinceChina

BACKGROUND Fibrous dysplasia(FD)is a common benign intramedullary fibro-osseous *** of the spine is rare,with the literature including only case reports,and cases of monostotic FD(MFD)in the sacrum are extremely rare.A correct preoperative diagnosis of spinal MFD is important for clinicians to select proper *** SUMMARY We retrospectively assessed a case report of MFD in the *** patient was examined by computed tomography(CT)and magnetic resonance imaging(MRI),and the diagnosis was confirmed by pathology.A review of the literature was performed to analyze the imaging characteristics and differential diagnoses of spinal *** our patient,the CT scan showed the lesion to be expansile,with ground glass opacity and a sclerotic *** MRI,the lesion showed iso-low signal intensity on T1WI and iso-high signal intensity on T2WI.A low signal rim was found on T1WI and *** patient was treated by posterior focal excision,decompression,bone grafting,fusion and pedicle screw fixation.A satisfactory result was achieved,with pain *** complications had occurred at the 1-year follow *** MFD is an expansile osteolytic *** glass opacity and a sclerotic margin are obvious *** lesion often involves the vertebral body and posterior *** of these imaging characteristics of spinal FD could be helpful for diagnosis and prevent unnecessary procedures.

关键词： Monostotic Sacrum Fibrous dysplasia Spine Computed tomographic Case report Magnetic resonance imaging

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餐厨垃圾和剩余污泥协同处理研究进展

广州化工 2024年第6期52卷 33-35页

作者：黄佳梦李珍妮陈虹颖于红艳台州职业技术学院浙江台州318000

餐厨垃圾和剩余污泥单独处理效率较低,协同处理可以更加高效地资源化利用。本文通过对好氧堆肥、厌氧消化、焚烧/热解等技术协同处理餐厨垃圾和剩余污泥的进展进行分析,探讨了物料比、抑制物质如脂质、盐分、重金属,添加物质如有益菌、... 详细信息

餐厨垃圾和剩余污泥单独处理效率较低,协同处理可以更加高效地资源化利用。本文通过对好氧堆肥、厌氧消化、焚烧/热解等技术协同处理餐厨垃圾和剩余污泥的进展进行分析,探讨了物料比、抑制物质如脂质、盐分、重金属,添加物质如有益菌、生物炭、微量元素对餐厨垃圾和剩余污泥协同处理的效果,旨在为餐厨垃圾和剩余污泥资源化利用提供参考。

关键词：餐厨垃圾剩余污泥协同处理抑制物质添加物质