检索结果-南通市图书馆

Influence of Solvent Size on Structural Properties of Bottle-brush Polymers

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2010年第5期26卷 838-841页

作者： LIU Ying-tao LIU hong LIU yu-hua LU Zhong-yuan State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical Chemistry Jilin University Changchun 130021 P. R. China

We proposed a modified dissipative particle dynamics simulation model by which one can effectively avoid the bond-crossing problem, and investigated the effect of solvent size on the structural properties of bottle-brush polymers in dilute solution on the basis of this model. It was found that with the increase of solvent size, the radius of the gyration of the bottle-brush polymer decreases considerably in the athermal solvent but increases in the selective solvent favoring the backbone, respectively.

关键词： Dissipative particle dynamics Bottle-brush polymer Solvent size Bond-crossing

Absence of ephrin-A2/A3 increases retinal regenerative potential for Müller cells in Rhodopsin knockout mice

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第7期16卷 1317-1322页

作者： Rui-Lin Zhu yuan Fang hong-hua yu Dong FChen Liu Yang Kin-Sang Cho Department of Ophthalmology Peking University First HospitalBeijingChina Schepens Eye Research Institute Massachusetts Eye and EarHarvard Medical SchoolBostonMAUSA Department of Ophthalmology and Visual Science Eye&ENT HospitalShanghai Medical CollegeFudan UniversityShanghaiChina Guangdong Eye Institute Department of OphthalmologyGuangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouGuangdong ProvinceChina

Müller cells(MC) are considered dormant retinal progenitor cells in *** studies demonstrated ephrin-As act as negative regulators of neural progenitor cells in the retina and *** remains unclear whether the lack of ephrin-A2/A3 is sufficient to promote the neurogenic potential of *** we investigated whether the MC is the primary retinal cell type expressing ephrin-A2/A3 and their role on the neurogenic potential of Müller *** this study, we showed that ephrin-A2/A3 and their receptor EphA4 were expressed in retina and especially enriched in *** level of ephrin As/EphA4 expression increased as the retina matured that is correlated with the reduced proliferative and progenitor cell potential of ***, we investigated the proliferation in primary MC cultures isolated from wild-type and A2~(–/–) A3~(–/–) mice by 5-ethynyl-2′-deoxyuridine(EdU) *** detected a significant increase of EdU~+ cells in MC derived from A2~(–/–) A3~(–/–) ***, we investigated the role of ephrin-A2/A3 in mice undergoing photoreceptor degeneration such as Rhodopsin knockout(Rho~(–/–)) *** further evaluate the role of ephrin-A2/A3 in MC proliferation in vivo, EdU was injected intraperitoneally to adult wild-type, A2~(–/–) A3~(–/–), Rho~(–/–) and Rho~(–/–) A2~(–/–) A3~(–/–) mice and the numbers of EdU~+ cells distributed among different layers of the *** As/EphA4 expression was upregulated in the retina of Rho~(–/–) mice compared to the wild-type *** addition, cultured MC derived from ephrin-A2~(–/–) A3~(–/–) mice also expressed higher levels of progenitor cell markers and exhibited higher proliferation potential than those from wild-type ***, we detected a significant increase of EdU~+ cells in the retinas of adult ephrin-A2~(–/–) A3~(–/–) mice mainly in the inner nuclear layer;and these EdU~+ cells were co-localized with MC marker, cellular retinaldehyde-binding protein, suggesting some proliferating cells are from

关键词： endogenous stem cell EphA4 ephrin-A2 ephrin-A3 ephrins Müller cell photoreceptor cell regeneration retinal degeneration retinal regeneration retinal stem cell

Identification of retinal thickness and blood flow in agerelated macular degeneration with reticular pseudodrusen

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2023年第8期16卷 1268-1273页

作者： Yong-Zhao Wei Hao huang Xiang Zhang hong-hua yu Bao-Yi Liu yu-Yao Diao Lu Cheng Hao Cheng Department of Ophthalmology the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120Guangdong ProvinceChina State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510275Guangdong ProvinceChina Guangdong Eye Institute Department of OphthalmologyGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhou 510080Guangdong ProvinceChina

AIM:To investigate thickness characteristics and vascular plexuses in retinas with reticular pseudodrusen(RPD)as an early detection strategy for age-related macular degeneration(AMD).METHODS:This retrospective study included 24 subjects(33 eyes)with RPD and 25 heathy control subjects(34 eyes).The superficial capillary plexus(SCP)and the deep capillary plexus(DCP)of the retinal posterior poles were investigated with optical coherence tomography angiography(OCTA).Retinal thicknesses and vessel densities were analyzed ***:The general retinal thicknesses of RPD eyes were significantly decreased(95%CI-14.080,-0.655;P=0.032).The vessel densities of DCP in RPD eyes were significantly increased in the global(95%CI 1.067,7.312;P=0.027),parafoveal(95%CI 0.417,5.241;P=0.022),and perifoveal(95%CI 0.181,6.842;P=0.039)***,the vessel densities of the SCP were rarely increased in the eyes with ***:The thinning of retinas in the RPD group suggests a reduction in the number of ***,the increased vessel density of the DCP in retinas with RPD indicates a greater demand for blood supply,possibly due to the hypoxia induced RPD compensation caused by RPD in the outer *** study highlights the pathological risks associated with RPD and emphasizes the importance of early intervention to retard the progression of AMD.

关键词： reticular pseudodrusen age-related macular degeneration retinal thickness retinal vessel density optical coherence tomography angiography

Experimental study of interband and intraband crosstalk in WDM networks

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optoelectronics Letters 2008年第1期4卷 42-44页

作者： GUAN Ai-hong and WANG yu-hua College of Information Science and Technology, Henan University of Technology, Zhengzhou 450001,China College of Information Science and Technology Henan University of Technology Zhengzhou 450001 China

The impacts of interband and intraband crosstalk are studied and compared experimentally. Results show that interband crosstalk can be removed with narrow-band filters and has no influence on signal. Intraband crosstalk will result in signal eye diagram close and BER increasing. When the polarization states of signal and crosstalk align, intraband crosstalk seriously decreases signal quality. But when they misalign, it has little influence. Coherent and incoherent crosstalk are studied experimentally. Results show that coherent crosstalk is less harmful to system performance than incoherent crosstalk.

关键词：光纤通信波长分组复用网络串话干扰实验研究

Effects of Inhibiting JAK on Invasion and Metastasis of the Human Breast Cancer Cells through ERK Signaling Transduction Pathway

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2009年第1期21卷 32-36页

作者： Jing Zhao hong-fang Chen hua-yu Deng Department of Pathophysiology Chongqing Medical University Chonging 400016 China

Objective： To explore the effects of Janus activated kinase （JAK） inhibitor AG490 on the phosphorylation of extracellular signal regulated protein kinase （ERK） in human breast cancer cells MDA-MB-231 and the roles of JAK in the invasion and metastasis of the human breast cancer cells through ERK signaling transduction pathways. Methods： MDA-MB-231 cells were treated with 20 μmol/L, 40 μmol/L, 80 μmol/L Janus kinase inhibitor AG490 for 24, 48 and 72 h. Proliferation and adhesion of MDA-MB-231 cells to matrigel were measured with MTT assay. When treated with 40 μmol/L AG490 for 24 h, the expressions of P-ERK and MMP-9 of cells were detected by Western-blot and invasion and metastasis of MDA-MB-231 cells were evaluated with transwell chamber. Results： After being treated with 20 μmol/L, 40 μ/L, 80 μmol/L AG490 for 24, 48 and 72 h, the proliferation of MDA-MB-231 cells was inhibited in a dose-and time-dependent manner. MDA-MB-231 cells treated with 40 μmol/L AG490 for 30, 60, 90 and 120 rain resulted in the increasing adhesion of cells to Matrigel in a time-dependent manner. However, capacity of adhesion in the group treated with AG490 was significantly decreased in comparison with the control group （P〈0.01）. The expression level of P-ERK and MMP-9 were decreased when treated with AG490. After treatment with 40 μmol/L AG490, in invasion assay, the number of cells in AG490 treated group to migrate to filter coated with Matrigel was reduced compared with control group （P〈0.05） Meanwhile, in migration assay, the number of cells in AG490 treated group to migrate to filter was also decreased compared with control group （P〈0.05）. Conclusion： Our study indicates that JAK kinase could affect the activity of ERK signal transduction pathway through the phosphorylation of ERK. The inhibitory effects of JAK kinase on MMP-9 expression and invasion of breast cancer cells were associated with the down-regulation of the ERK signaling pathway.

关键词： Breast cancer JAK ERK Invasion Metastasis

Protective effects of lipoic acid-niacin dimers against blue light-induced oxidative damage to retinal pigment epithelium cells

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2019年第8期12卷 1262-1271页

作者： Xiu-Lan Zou Yong-Zhen yu hong-hua yu Guan-Feng Wang Rong-Biao Pi Zhe Xu Chu Zhang Wen-Jie Zhou Dan-Dan Li Xuan-Ge Chen yu-Ping Zou Department of Ophthalmology General Hospital of SouthernTheatre Command of PLAGuangzhou510010GuangdongProvinceChina Department of Ophthalmology Guangdong ProvincialPeople’s HospitalGuangzhou510000Guangdong ProvinceChina Department of Ophthalmology Third Affiliated Hospitalof Guangzhou Medical UniversityGuangzhou510000Guangdong ProvinceChina School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou510000Guangdong ProvinceChina Department of Ophthalmology the First Affiliated HospitalGuangdong Pharmaceutical UniversityGuangzhou510000Guangdong ProvinceChina Zhongshan Ophthalmic Centre of Sun Yat-sen University Guangzhou510000Guangdong ProvinceChina

AIM: To evaluate the protective effects of lipoic acid-niacin(N2 L) dimers against blue light(BL)-induced oxidative damage to human retinal pigment epithelium(hRPE) cells in ***: h RPE cells were divided into a control group(CG), a BL group, an N2 L plus BL irradiation group, an α-lipoic acid(ALA) plus BL group, an ALA-only group, and an N2 L-only group. hRPE cellular viability was detected by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) bromide assays, and apoptosis was evaluated by annexin-V-PE/7-AAD staining followed by flow cytometry. Ultrastructural changes in subcellular organelles were observed by transmission electron microscopy. Reactive oxygen species formation was assayed by flow cytometry. The expression levels of the apoptosis-related proteins BCL-2 associated X protein(BAX), B-cell leukmia/lymphoma 2(BCL-2), and caspase-3 were quantified by Western blot ***: BL exposure with a light density of 4±0.5 mW/cm2 exceeding 6 h caused hRPE toxicity, whereas treatment with a high dose of N2 L(100 mol/L) or ALA(150 mol/L) maintained cell viability at control levels. BL exposure caused vacuole-like degeneration, mitochondrial swelling, and reduced microvillus formation;however, a high dose of N2 L or ALA maintained the ultrastructure of hRPE cells and their organelles. High doses of N2 L and ALA also protected hRPE cells from BL-induced apoptosis, which was confirmed by Western blot analysis: BCL-2 expression significantly increased, while BAX and caspase-3 expression slightly decreased compared to the ***: High-dose N2 L treatment(>100 mol/L) can reduce oxidative damage in degenerating hRPE cells exposed to BL with an efficacy similar to ALA.

关键词： lipoic acid-niacin dimers retinal pigment epithelium cell lipoic acid oxidative stress reactive oxygen species apoptosis

LC-MS determination and pharmacokinetic study of salidroside in rat plasma after oral administration of suspensions of traditional Chinese medicine Erzhi Wan and Fructus Ligustri lucidi

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2011年第1期1卷 8-12页

作者： Ze-yun Li Qing Li Jiang Lu Jun-hong Ling Xi-hua yu Xiao-Hui Chen Kai-Shun Bi School of Pharmacy Shenyang Pharmaceutical University Shenyang 110016 China.

A simple,rapid and sensitive liquid chromatography-mass spectrometry（LC-MS）method was developed for the determination of salidroside in rat plasma and study of its pharmacokinetics after oral administration of suspension of Erzhi Wan and Fructus Ligustri lucidi into Wistar *** sample of 200 μL was extracted with acetic ether-isopropanol（2∶1）and the extraction was performed on a Kromasil C18 column（150 mm×4.6 mm,5 μm）with the mobile phase of methanol-water（41∶59,v/v）within a run time of 6.0 *** analyte was monitored with positive electrospray ionization（ESI）by selected ion monitoring（SIM）*** target ions were m/z 323.05 for salidroside and m/z 411.05 for internal standard（IS）geniposide.A good linear relationship was obtained over the range of 5.0-500.0 ng/mL and the lower limit of quantification was 5.0 ng/*** validated method was successfully applied to the pharmacokinetic study of salidroside in rat plasma after oral administration of suspension of Erzhi Wan and Fructus Ligustri lucidi.

关键词： liquid chromatography-mass spectrometry(LC-MS) pharmacokinetics salidroside Erzhi Wan Fructus Ligustri lucidi

在线全文

学校读者我要写书评

暂无评论

中药治疗缺血性视神经病变的临床研究

国际眼科杂志 2009年第2期9卷 311-312页

作者：余洪华广州军区广州总医院眼科中国广东省广州市510010

目的:探讨以行气疏肝为主的中药治疗缺血性视神经病变的临床效果。方法:将纳入缺血性视神经病变诊断的患者76例(102眼)随机分为两组:治疗组37例(51眼)以行气疏肝为主的中药汤剂口服,1剂/d,2wk为1个疗程,共4个疗程;对照组39例(51眼)复方... 详细信息

目的:探讨以行气疏肝为主的中药治疗缺血性视神经病变的临床效果。方法:将纳入缺血性视神经病变诊断的患者76例(102眼)随机分为两组:治疗组37例(51眼)以行气疏肝为主的中药汤剂口服,1剂/d,2wk为1个疗程,共4个疗程;对照组39例(51眼)复方血栓通胶囊口服,3粒/次,3次/d,2wk为1个疗程,共4个疗程。观察治疗前后视力、视野的改变,评价临床疗效。结果:4个疗程(2mo)的结果显示:治疗组总有效率88.2%,总显效率13.7%,对照组总有效率72.5%,总显效率9.8%。两者比较差异有显著性意义(χ2=2.733,P<0.05),治疗组治疗后裸眼视力及视野改善情况均优于对照组。结论:以行气疏肝为主的中药治疗缺血性视神经病变能有效地改善患者的视力、视野,具有良好的临床疗效。

关键词：缺血性视神经病变行气疏肝中药

含不同浓度氟化钠的酸蚀液对托槽抗剪粘接强度的影响

在线全文

学校读者我要写书评

暂无评论

华西口腔医学杂志 2005年第5期23卷 397-399页

作者：马嘉张扬洪玉华中国医科大学附属口腔医院正畸科辽宁沈阳110002 泰山齿科正畸科辽宁沈阳110032

目的在确保托槽粘接强度的条件下,探讨35%磷酸酸蚀液中添加氟化钠的最佳浓度。方法64颗离体牙随机分成A、B、C组和对照组,分别采用添加4种不同浓度氟化钠(1.23%、2%、3%和0%)的酸蚀液进行酸蚀并常规粘接托槽,于1周、4周、12周和24周分... 详细信息

目的在确保托槽粘接强度的条件下,探讨35%磷酸酸蚀液中添加氟化钠的最佳浓度。方法64颗离体牙随机分成A、B、C组和对照组,分别采用添加4种不同浓度氟化钠(1.23%、2%、3%和0%)的酸蚀液进行酸蚀并常规粘接托槽,于1周、4周、12周和24周分别从每组样本中随机抽取4颗牙进行抗剪粘接强度测试,并观测托槽脱落后界面粘接剂残留情况,记录粘接材料残留指数(ARI)。结果A、B、C组和对照组的抗剪粘接强度在4个观测时间点进行组内比较均无统计学差异,组间比较则有统计学差异,C组低于其他3组(P<0.05)。4组间ARI分值无统计学意义。结论35%磷酸酸蚀液中添加不高于2%的氟化钠,与单纯应用35%磷酸的粘接效果相似;当氟化钠浓度高于3%后,托槽的抗剪粘接强度下降。

关键词：氟化钠酸蚀抗剪粘接强度