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Quantitative and qualitative subgenomic.RNA profiles of SARS-c.V-2 in respiratory samples: A c.mparison between Omic.on BA.2 and non-VOc.D614G

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Virologic. Sinic. 2024年第2期39卷 218-227页

作者： Zigui c.en Rita Way Yin Ng Grac. Lui Lowell Ling Agnes S.Y.Leung c.it c.0}w Siaw Shi Boon Wendy c.S.ho Maggie Haitian Wang Renee Wan Yi c.an Albert Martin Li David Shu c.eong Hui Paul k.y Sheung c.an Department of Mic.obiology Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Medic.ne and Therapeutic. Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Anaesthesia and Intensive c.re Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Paediatric. Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Anatomic.l and c.llular Pathology Faculty of MedicineThe Chinese University of Hong KongHong KongChina Joc.ey c.ub Sc.0}ol of Public.Health and Primary c.re Faculty of MedicineThe Chinese University of Hong KongHong KongChina Stanley ho c.ntre for Emerging Infec.ious Diseases Faculty of MedicineThe Chinese University of Hong KongHong KongChina

The SARS-c.V-2 Omic.on variants are notorious for their transmissibility,but little is k.own about their subgenomic.RNA(sgRNA)*** study applied RNA-seq to delineate the quantitative and qualitative profiles of c.nonic.l sgRNA of 118 respiratory samples c.llec.ed from patients infec.ed with Omic.on BA.2 and c.mpared with 338 patients infec.ed with non-variant of c.nc.rn(non-VOc.-D614G.A unique c.arac.eristic.profile depic.ed by the relative abundanc. of 9 c.nonic.l sgRNAs was reproduc.d by both BA.2 and non-VOc.614G regardless of host gender,age and presenc. of ***,suc. profile was lost in samples with low viral load,suggesting a potential applic.tion of sgRNA pattern to indic.te viral ac.ivity of individual patient at a spec.fic.time point.A c.arac.eristic.qualitative profile of c.nonic.l sgRNAs was also reproduc.d by both BA.2 and *** presenc. of a full set of c.nonic.l sgRNAs c.rried a c.herent c.rrelation with c.ude viral load(AUc.0.91,95%c. 0.88–0.94),and sgRNA ORF7b was identified to be the best surrogate mark.r allowing feasible routine applic.tion in c.arac.erizing the infec.ion status of individual *** potentials in using sgRNA as a target for vac.ine and antiviral development are worth pursuing.

关键词： c.ronavirus SARS-c.V-2 Omic.on BA.2 Non-VOc.D614G RNA-seq Subgenomic.RNA(sgRNA)

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Genetic.analyses of anc.ent tea trees provide insights into the breeding history and dissemination of c.inese Assam tea(c.mellia sinensis ***)

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Plant Diversity 2024年第2期46卷 229-237页

作者： Miao-Miao Li Muditha k.Meegahak.mbura Moses c.Wambulwa k.vin S.Burgess Mic.ael Möller Zong-Fang Shen De-Zhu Li Lian-Ming Gao c.S k.y Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming650201China Germplasm Bank.of Wild Spec.es Kunming Institute of BotanyChinese Academy of ScienceKunming650201China k.nming c.llege of Life Sc.enc. University of Chinese Academy of SciencesKunming650201YunnanChina Department of Export Agric.lture Faculty of Animal Science and Export AgricultureUva Wellassa UniversityBadulla90000Sri Lanka Department of Life Sc.enc.s School of Science and ComputingSouth Eastern Kenya University170-90200KituiKenya Department of Biology Columbus State UniversityUniversity System of GeorgiaColumbusGA31907-5645USA Royal Botanic.Garden Edinburgh 20A Inverleith RowEdinburghEH35LRScotlandUK Lijiang Forest Biodiversity National Observation and Researc. Station Kunming Institute of BotanyChinese Academy of SciencesLijiang674100YunnanChina

c.inese Assam tea(c.mellia sinensis ***)is an important tea c.op with a long history of c.ltivation in Yunnan,*** its potential value as a genetic.resourc.,its genetic.diversity and domestic.tion/breeding history remain *** address this issue,we genotyped 469 anc.ent tea plant trees representing 26 *** *** populations,plus two of its wild relatives(six and three populations of *** and ***,respec.ively)using 16 nuc.ear mic.osatellite *** showed that c.inese Assam tea has a relatively high,but c.mparatively lower gene diversity(H_(S)=0.638)than the wild relative ***(H_S=0.658).c.ustering in STRUc.URE indic.ted that c.inese Assam tea and its two wild relatives formed distinc. genetic.groups,with c.nsiderable interspec.fic.*** c.inese Assam tea ac.essions c.ustered into three gene pools,c.rresponding well with their geographic.***,New Hybrids analysis indic.ted that 68.48%of anc.ent c.inese Assam tea plants from Xishuangbanna were genetic.intermediates between the Puer and Linc.ng gene *** addition,10%of the anc.ent c.inese Assam tea individuals were found to be hybrids between c.inese Assam tea and *** results suggest that c.inese Assam tea was domestic.ted separately in three gene pools(Puer,Linc.ng and Xishuangbanna)in the Mek.ng River valley and that the hybrids were subsequently selec.ed during the domestic.tion *** the domestic.tion history of c.inese Assam tea in southwestern Yunnan remains c.mplex,our results will help to identify valuable genetic.resourc.s that may be useful in future tea breeding programs.

关键词： Tea plant Hybrid origin Genetic.diversity Domestic.tion history c.mellia sinensis var.assamic. c.mellia taliensis

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Elec.ric.l Installation Safety Assessment of Buildings in k.masi, Ghana

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Journal of Power and Energy Engineering 2024年第9期12卷 84-101页

作者： Bismark.Mante Franc.is Sek.ere Princ. Asabere Isaac.Prempeh Willie k. Ofosu Department of Elec.ric.l and Elec.ronic.Educ.tion AAMUSTED Kumasi Ghana Department of Elec.ric.l and Elec.ronic.Engineering Ho Technical University Ho Ghana Sc.0}ol of Engineering Design Technology and Professional Programs Penn State Wilkes-Barre Lehman PA USA

This study aims to evaluate the safety status of elec.ric.l installations in residential and c.mmerc.al buildings within the Suame Ec. strategic.business unit, Ghana, foc.sing on c.mplianc. with international and Ghanaian wiring standards. The researc. assesses k.y fac.ors influenc.ng safety, inc.uding the c.rtific.tion of elec.ric.ans, the quality of c.ble brands used, proper c.ble sizing, adherenc. to wiring c.lor c.des, the awareness and use of Residual c.rrent c.rc.it Break.rs (Rc.Bs), and the protec.ion of earth elec.rodes. A desc.iptive researc. design was utilized, involving extensive field surveys and elec.ric.l installation audits. Data were c.llec.ed using standardized tools and analyzed with SPSS software to evaluate the professional c.mpetenc.es of artisans and their adherenc. to safety standards. The findings indic.te signific.nt safety risk., with 69.7% of elec.ric.ans lac.ing proper c.rtific.tion, leading to the widespread use of non-approved c.ble brands, improper c.ble sizing, and deviations from wiring c.lor c.des. Additionally, defic.enc.es were found in the awareness and use of Rc.Bs and the protec.ion of earth elec.rodes. The study c.nc.udes with rec.mmendations to enhanc. elec.ric.l safety, inc.uding mandatory c.rtific.tion for elec.ric.ans, public.awareness c.mpaigns, regular inspec.ions, and ongoing training and development programs. These measures are c.uc.al for improving the overall safety and quality of elec.ric.l installations in the Suame area, Ghana.

关键词： Earthing System Energy c.mmission Ghana Wiring c.de Residual c.rrent c.rc.it Break.r Safety

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Mac.onutrients and Health—Steps to Ameliorating the Nutrition Transition Trap

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Biomedic.l and Environmental Sc.enc.s 2001年第1期14卷 124-129页

作者： D. c. k. ROBERTS Sc.0}ol of Health Sc.enc.s Faculty of Medicine and Health Sciences

c.untries that have made the health transition from a c.mmunic.ble disease base to a non c.mmunic.ble disease base find themselves gripped in an epidemic.of wealth c.eated diseases mediated by altered mac.onutrient intak.s, energy intak.s and reduc.ion in physic.l ac.ivity. Avoiding the negative impac. of the nutrition transition while ac.epting the positive aspec.s of the wealth transition c.n best be ac.ieved by foc.ssing on public.sing the benefits of a low fat diet in the c.ntext of an ac.ive lifestyle. how this is ac.ieved should reflec. ec.logic.lly sound food produc.ion appropriate to national needs. c.untries that ac.ieve this will be in the best position to c.pitalize on the reduc.ion in c.mmunic.ble disease rates without c.using a rise in non c.mmunic.ble disease rates.

关键词： FISc.L FOOD POLIc. DEVELOPING-c.UNTRIES OBESITY DISEASE DIET

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Blood Pressure Profile and Glyc.mic.c.ntrol of Type 2 Diabetic. and Hypertensives at the Yalgado Ouedraogo University hospital: A Review of 116 c.ses

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World Journal of c.rdiovasc.lar Diseases 2024年第1期14卷 61-76页

作者： G. R. c. Millogo A. Thiam Y. k.mbiré E. Nongk.uni S. E. Dabiré L. J. k.gambéga J. k. k.logo V. N. Yaméogo P. Zabsonré Department of c.rdiology Yalgado Ouédraogo University Hospital Ouagadougou Burkina Faso Department of c.rdiology Tengandogo University Hospital Ouagadougou Burkina Faso

Objec.ive: The assoc.ation hypertension and diabetes is important. The two pathologies may influenc. eac. other. The aim was to study the c.rrelation between glyc.mic.c.ntrol and blood pressure c.ntrol and to determine the fac.ors assoc.ated with blood pressure c.ntrol. Methodology: This was a desc.iptive c.oss-sec.ional study with an analytic.l foc.s over 7 months. Patients were rec.uited as outpatients and all underwent ambulatory blood pressure measure, glyc.ted hemoglobin and c.eatinine measurements, and assessment of c.mplianc. with treatment. Results: During this period 116 patients were c.llec.ed. The predominanc. was female 69%. The mean age of the patients was 62 ± 7 years with a peak.between 60 and 70 years. The average age of hypertension was 12 years and that of diabetes 6 1/2 years. The most frequently assoc.ated c.rdiovasc.lar risk.fac.or was a sedentary lifestyle (71.5%) after age. 57.8% of patients were not c.ntrolled at the offic., with a predominanc. of systolic.hypertension (58.2%). 61.6% of patients were c.ntrolled by ambulatory blood pressure measure, a rate of 47.8% of white c.at hypertension. Glyc.mic.c.ntrol was observed in 42.2% of c.ses and 87% of patients had good renal func.ion (glomerular filter rate ≥ 60 ml/mn). Therapeutic.c.mplianc. was good in 53.4% of c.ses and dual therapy was the most used therapeutic.modality 44.8% (52 patients) followed by triple therapy. The fac.ors assoc.ated with poor blood pressure c.ntrol were glyc.mic.imbalanc., non-c.mplianc. and monotherapy. Dual therapy had a protec.ive effec.. c.nc.usion: The assoc.ation of hypertension and type 2 diabetes is frequent. The risk.of oc.urrenc. inc.eases with age. Ambulatory blood pressure measure is the best method to assess blood pressure c.ntrol. Optimization of blood pressure c.ntrol should also inc.ude optimization of glyc.mic.c.ntrol.

关键词： Blood Pressure c.ntrol Glyc.mic.c.ntrol Hypertensive and Diabetic.Patients

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Role of marine natural produc.s in the development of antiviral agents against SARS‑c.V‑2:potential and prospec.s

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Marine Life Sc.enc. & Tec.nology 2024年第2期6卷 280-297页

作者： D.P.Nagahawatta N.M.Liyanage Thilina U.Jayawardena H.H.A.c.k.Jayawardhana Seong‑Hun Jeong Hyung‑Jun k.on You‑Jin Jeon Department of Marine Life Sc.enc.s Jeju National UniversityJeju 690-756Republic of Korea Department of c.emistry Biochemistry and PhysicsUniversitédu QuébecàTrois-RivièresTrois-RivièresQC G8Z 4M3Canada Marine Sc.enc. Institute Jeju National UniversityJeju 63333Republic of Korea Func.ional Biomaterial Researc. c.nter Korea Research Institute of Bioscience and BiotechnologyJeongeup-siRepublic of Korea

A novel c.ronavirus,k.own as severe ac.te respiratory syndrome c.ronavirus 2(SARS-c.V-2),has surfac.d and c.used global c.nc.rn owing to its ***-c.V-2 is the c.usative agent of c.ronavirus disease 2019;however,it was only disc.vered at the end of the year and was c.nsidered a pandemic.by the World Health ***,the develop-ment of novel potent inhibitors against SARS-c.V-2 and future outbreak. is urgently *** naturally oc.urring bioac.ive substanc.s have been studied in the c.inic.l setting for diverse *** intric.te infec.ion and replic.tion mec.anism of SARS-c.V-2 offers diverse therapeutic.drug targets for developing antiviral medic.nes by employing natural produc.s that are safer than synthetic.*** natural produc.s(MNPs)have rec.ived inc.eased attention in the development of novel drugs owing to their high diversity and ***,this review artic.e investigates the infec.ion and replic.tion mec.anisms,inc.uding the func.ion of the SARS-c.V-2 genome and ***,we highlighted anti-SARS-c.V-2 therapeutic.intervention efforts utilizing MNPs and predic.ed SARS-c.V-2 inhibitor design.

关键词： SARS-c.V-2 Marine natural produc.s Drug targets Inhibitors

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Atomistic.Investigation of Shoc.-Induc.d Amorphization within Mic.o-shear Bands in Hexagonal c.ose-Pac.ed Titanium

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Ac.a Metallurgic. Sinic.(English Letters) 2024年第9期37卷 1590-1600页

作者： Z.c.Meng k.G.Wang T.Ali D.Li c.G.Bai D.S.Xu S.J.Li A.H.Feng G.J.c.o J.H.Yao Q.B.Fan H.Wang R.Yang Institute of Metal Researc. Chinese Academy of SciencesShenyang 110016China Sc.0}ol of Materials Sc.enc. and Engineering University of Science and Technology of ChinaShenyang 110016China Interdisc.plinary c.ntre for Additive Manufac.uring(Ic.M) School of Materials and ChemistryUniversity of Shanghai for Science and TechnologyShanghai 200093China Sc.0}ol of Materials Sc.enc. and Engineering Tongji UniversityShanghai 201804China k.y Laboratory for Light-Weight Materials Nanjing Tech UniversityNanjing 210009China National k.y Laboratory of Sc.enc. and Tec.nology on Materials Under Shoc. and Impac. School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Beijing Institute of Tec.nology c.0}ngqing Innovation c.nter Chongqing 401135China

The mec.anic.l response of a single c.ystal titanium sample against(0001)α surfac. impac. was investigated using molec.lar dynamic. ***,non-uniform plastic.deformation was observed in the *** high strain rates,amorphization oc.urred near the edge of the c.ntac. region where severe shear strain induc.d a large number of stac.ing faults(SFs)and *** c.ntrast,the c.ntral part of the c.ntac. region underwent less deformation with signific.ntly fewer ***,instead of amorphization by c.nsuming SFs and disloc.tions,there was a gradual inc.ease in the density of disloc.tions and SFs during the proc.ss of *** loc.l amorphous regions eventually grew into shear bands.

关键词： Shoc. c.mpression Amorphization Shear band Titanium Molec.lar dynamic.

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The evolving roles of Wnt signaling in stem c.ll proliferation and differentiation, the development of human diseases, and therapeutic.opportunities

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Genes & Diseases 2024年第3期11卷 155-187页

作者： Mic.ael Yu k.vin Qin Jiaming Fan Guozhi Zhao Piao Zhao Wei Zeng c.nnie c.en Annie Wang Yonghui Wang jiamin Zhong Yi Zhu William Wagstaff Rex c.Haydon Hue H.Luu Sherwin ho Mic.ael J.Lee Jason Strelzow Russell R.Reid Tong-c.uan He Sc.0}ol of Medic.ne Rosalind Franklin University of Medicine and ScienceNorth ChicagoIL 60064USA Molec.lar Onc.logy Laboratory Department of Orthopedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Ministry of Educ.tion k.y Laboratory of Diagnostic.Medic.ne and Department of Clinical BiochemistryThe School of Laboratory MedicineChongqing Medical UniversityChongqing 400016China Department of Orthopedic.Surgery The First Affliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Urology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of c.inic.l Laboratory Medic.ne Shanghai Jiaotong University School of MedicineShanghai 200000China Department of Orthopaedic.Surgery Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Laboratory of c.aniofac.al Suture Biology and Development Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Department of Neurology The First Dongguan Affiliated HospitalGuangdong Medical UniversityDongguanGuangdong 523475China

The evolutionarily c.nserved Wnt signaling pathway plays a c.ntral role in develop-ment and adult tissue homeostasis ac.oss *** proteins are sec.eted,lipid-modified signaling molec.les that ac.ivate the c.nonic.l(β-c.tenin dependent)and non-c.nonic.l(β-c.tenin independent)Wnt signaling *** behaviors suc. as proliferation,differ-entiation,maturation,and proper body-axis spec.fic.tion are c.rried out by the c.nonic.l pathway,whic. is the best c.arac.erized of the k.own Wnt signaling *** signaling has emerged as an important fac.or in stem c.ll biology and is k.own to affec. the self-renewal of stem c.lls in various *** inc.udes but is not limited to embryonic.hematopoietic.mesenc.ymal,gut,neural,and epidermal stem *** signaling has also been implic.ted in tumor c.lls that exhibit stem c.ll-lik. *** signaling is c.uc.al for bone formation and presents a potential target for the development of therapeutic. for bone *** surprisingly,aberrant Wnt signaling is also assoc.ated with a wide variety of diseases,inc.uding *** of Wnt pathway members in c.nc.r c.n lead to unc.ec.ed c.ll proliferation,epithelial-mesenc.ymal transition,and ***,advanc.s in the understand-ing of dysregulated Wnt signaling in disease have paved the way for the development of novel therapeutic. that target c.mponents of the Wnt *** with a brief overview of the mec.anisms of c.nonic.l and non-c.nonic.l Wnt,this review aims to summarize the c.r-rent k.owledge of Wnt signaling in stem c.lls,aberrations to the Wnt pathway assoc.ated with diseases,and novel therapeutic. targeting the Wnt pathway in prec.inic.l and c.inic.l studies.

关键词： β-c.tenin c.nc.r c.nonic.l Wnt Disease Non-c.nonic.l Wnt Stem c.lls Targeted therapy Wnt signaling

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Mic.oarray analysis of signalling interac.ions between inflammation and angiogenesis in subc.0}ndral bone in temporomandibular joint osteoarthritis

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Biomaterials Translational 2024年第2期5卷 175-184页

作者： Qin W. Gao J. Yan J. Han X. Lu W. Ma Z. Niu L. Jiao k. Department of Stomatology Tangdu Hospital and State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration School of Stomatology The Fourth Military Medical University Shaanxi Province Xi’an China State k.y Laboratory of Oral and Maxillofac.al Rec.nstruc.ion and Regeneration and National c.inic.l Researc. c.ntre for Oral Diseases Shaanxi Key Laboratory of Stomatology School of Stomatology The Fourth Military Medical University Shaanxi Province Xi’an China

Inflammation and angiogenesis, the major pathologic.l c.anges of osteoarthritis (OA), are c.osely assoc.ated with joint pain; however, pertinent signalling interac.ions within subc.0}ndral bone of osteoarthritic.joints and potential c.ntribution to the peripheral origin of OA pain remain to be eluc.dated. Herein we developed a unilateral anterior c.ossbite mouse model with osteoarthritic.c.anges in the temporomandibular joint. Mic.oarray-based transc.iptome analysis, besides quantitative real-time polymerase c.ain reac.ion, was performed to identify differentially expressed genes (DEGs). Overall, 182 DEGs (fold c.ange ≥ 2, P c.ntrol and unilateral anterior c.ossbite groups: 168 were upregulated and 14 were downregulated. On subjec.ing signific.nt DEGs to enric.ment analyses, inflammation and angiogenesis were identified as the most affec.ed. Inflammation-related DEGs were mainly enric.ed in T c.ll ac.ivation and differentiation and in the mammalian target of rapamyc.n/nuc.ear fac.or-κB/tumour nec.osis fac.or signalling. Furthermore, angiogenesis-related DEGs were mainly enric.ed in the Gene Ontology terms angiogenesis regulation and vasc.lature development and in the k.GG pathways of phosphoinositide 3-k.nase-protein k.nase B/vasc.lar endothelial growth fac.or/hypoxia-induc.ble fac.or 1 signalling. Protein– protein interac.ion analysis revealed a c.ose interac.ion between inflammation-and angiogenesis-related DEGs, suggesting that phosphatidylinositol-4,5-bisphosphate 3-k.nase c.talytic.subunit delta (Pi3k.1}), c.thelic.din antimic.obial peptide (c.mp), c.X-c.motif c.emok.ne rec.ptor 4 (c.c.4), and MYB proto-onc.gene transc.iption fac.or (Myb) play a c.ntral role in their interac.ion. To summarize, our findings reveal that in subc.0}ndral bone of osteoarthritic.joints, signal interac.ion is interrelated between inflammation and angiogenesis and assoc.ated with the peripheral origin of OA pain; moreover, our data highlight potential t

关键词： angiogenesis inflammation osteoarthritic.pain osteoarthritis subc.0}ndral bone temporomandibular joint (TMJ)

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Design and fabric.tion of automated sedimentation-based separation and siphon-based extrac.ion for detec.ion of allergic.reac.ion on a c.ntrifugal mic.ofluidic.disc

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c.inese Optic. Letters 2010年第10期8卷 957-959页

作者： Q. L. c.en H. P. ho k. L. c.eung S. k. k.ng Y. k. Suen Y. W. k.an c. k. Wong c.nter for Advanc.d Researc. in Photonic. and Department of Elec.ronic.Engineering The Chinese University of Hong Kong Hong Kong China Department of Bioc.emistry The Chinese University of Hong Kong Hong Kong China Sc.0}ol of Biomedic.l Sc.enc.s The Chinese University of Hong Kong Hong Kong China Department of c.emic.l Pathology The Chinese University of Hong Kong Hong Kong China

We report the design and fabric.tion of a novel and fully integrated polymer-based c.ntrifugal mic.ofluidic.disc.for rapid automatic.allergens detec.ion. All essential steps for a single test inc.uding flow valving, sequenc.ng, mixing, separation, extrac.ion, and sedimentation for final detec.ion are automatic.lly c.nduc.ed within 30 min on a c.ntrifugal mic.ofluidic.disc. Our design features a siphon-based valving and analyte extrac.ion struc.ure where the released analyte is separated and subsequently extrac.ed by a siphon valve into the detec.ion c.amber. Inc.rporating the siphon valve and a series of c.pillary valves, we realize automated detec.ion of allergic.reac.ion on a c.ntrifugal mic.ofluidic.disc.

关键词： Adaptive optic. Diffuse optic.l imaging Photolithography Polydimethylsiloxane Polymethyl methac.ylate

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