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A new simple technique of epididymal sperm collection for obstructive azoospermia

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Asian Journal of Andrology 2016年第1期18卷 149-150页

作者： Tatsuo Morita Maiko Komatsubara Tomohiro Kameda Ai Morikawa Taro Kubo Akira Fujisaki Shinsuke Kurokawa Hirotoshi kawata Akira Tanaka Department of Urology Jichi Medical University Shimotsuke-shi Tochigi Japan Department of Pathology Jichi Medical University Shimotsuke-shi Tochigi Japan

Dear Editor,Sperm in patients with obstructive azoospermia （OA） have been retrieved from the reproductive tract and/or from the testis for intracytoplasmic sperm injection （ICSI） in several ways, including microsurgical epididymal sperm aspiration （MESA）, percutaneous epididymal sperm aspiration （PESA）, testicular sperm extraction （TESE）, and other techniques） Each of these has advantages and drawbacks in terms of microsurgical expertise or equipment, number of sperm obtained, invasiveness, and cost. Therefore, we developed a safe, simple, feasible, and low-cost modified MESA technique to collect epididymal sperm under direct vision, which we have labeled macrosurgical epididymal sperm imprint collection （MESIC）. Herein, we present the details of the MESIC technique and our initial experience in patients with suspected OA.

关键词： RETRIEVAL TECHNIQUES ASPIRATION OUTCOMES INJECTION

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耐热/耐磨烧结合金发展趋势

粉末冶金技术 2011年第5期29卷 386-393页

作者： Hideaki kawata Kunio Maki 韩凤麟日本日立粉末冶金(株) 亍270-2295松户市稔台520 不详

粉末冶金的一个特殊优势是合金设计自由度高,因此,烧结合金广泛用于耐热与耐磨零件。主要产品对象是气门导管、阀座圈(VSI)及发动机排气系统的零件。近年来,为回应环境问题,发动机的使用性能在不断提高,这些零件的使用环境随之越益严苛... 详细信息

粉末冶金的一个特殊优势是合金设计自由度高,因此,烧结合金广泛用于耐热与耐磨零件。主要产品对象是气门导管、阀座圈(VSI)及发动机排气系统的零件。近年来,为回应环境问题,发动机的使用性能在不断提高,这些零件的使用环境随之越益严苛,因此,就日益要求改进材料的耐磨性与其它性能。论述了近年来耐热/耐磨烧结材料的技术发展趋势,特别着重于材料发展趋势。

关键词：耐热性耐磨性切削性固体润滑剂阀系零件硬质颗粒

Dense pair plasma generation by two laser pulses colliding in a cylinder channel

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Chinese Physics B 2017年第3期26卷 361-368页

作者：刘建勋马燕云余同普赵军杨晓虎邹德滨张国博赵媛杨靖康李汉臻卓红斌邵福球 Shigeo kawata College of Science National University of Defense Technology Changsha 410073 China College of Electronic Engineering Wuhan 430019 China IFSA Collaborative Innovation Center Shanghai Jiao Tong University Shanghai 200240 China Laser Fusion Research Center China Academy of Engineering Physics Mianyang 621000 China Center for Optical Research and Education Graduate School of Engineering Utsunomiya University 7-1-2 Yohtoh Utsunomiya 321-8585 Japan

An all-optical scheme for high-density pair plasmas generation is proposed by two laser pulses colliding in a cylinder channel. Two dimensional particle-in-cell simulations show that, when the first laser pulse propagates in the cylinder, electrons are extracted out of the cylinder inner wall and accelerated to high energies. These energetic electrons later run into the second counter-propagating laser pulse, radiating a large amount of high-energy gamma photons via the Compton back-scattering process. The emitted gamma photons then collide with the second laser pulse to initiate the Breit-Wheeler process for pairs production. Due to the strong self-generated fields in the cylinder, positrons are confined in the channel to form dense pair plasmas. Totally, the maximum density of pair plasmas can be 4.60 × 10^27 m%-3, for lasers with an intensity of 4 × 10^22 ***^-2. Both the positron yield and density are tunable by changing the cylinder radius and the laser parameters. The generated dense pair plasmas can further facilitate investigations related to astrophysics and particle physics.

关键词： pair plasma laser pulse simulation

Design, Synthesis, Crystal Structure and Photoluminescence Properties of Four New Europium (III) Complexes with Fluorinated β-Diketone Ligand

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Crystal Structure Theory and Applications 2021年第1期10卷 1-13页

作者： Tetsuji Moriguchi Hiromasa kawata Venkataprasad Jalli Department of Applied Chemistry Faculty of Engineering Kyushu Institute of Technology Kitakyushu Japan Sankar Foundation Research and Development Division Visakhapatnam India

The strong photoluminescence properties of europium complexes with organic ligands attracted the attention of many researchers and found a wide range of uses in medical, industrial and biological fields. In this article, four new Tetrakis europium complexes 3a, 3b, 3c and 3d have been prepared using 1-phenyl-4,4,4-trifluoro-1,3-butenedionato ligand and pyridinium, bipyridinium, piperazinium and piperidinium counter cations. These complexes have been characterized by negative FAB-mass. The crystal structures of 3a, 3b, 3c and 3d were determined by single crystal X-ray diffraction analysis. The complex 3a crystallized in monoclinic form, space group P21/n with four molecules in the unit cell. The complex 3b crystallized in monoclinic form, space group P2/n with two complex molecules in the unit cell. The complex 3c crystallized in monoclinic form, space group C2/c with sixteen molecules in the unit cell. The complex 3d crystallized in monoclinic form, space group P21/n with four complex molecules in the unit cell. The complex 3a has 1,2-alternative structure, 3b has 1,3-alternative structure, 3c has cone like structure and 3d has partial cone like structure. The photoluminescence properties of these complexes have been evaluated. Strong red emissions were observed in all four complexes due to 5D0 → 7F2 transition of Europium (III) ions under UV excitation. Four β-diketone ligands acted as strong antenna ligands and transferred the absorbed energy to europium (III) ion effectively;consequently strong red luminescence was observed.

关键词： Tetrakis Europium Complexes Luminescence Fluorinate β-Diketone Ligand Pyridine Bipyridine Piperazine Bipiperidine

Generation of Unfolded DNA in Human Neutrophils Following Hypothermal Treatment

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CellBio 2013年第3期2卷 117-124页

作者： Jin kawata Makoto Kikuchi Hisato Saitoh Department of New Frontier Sciences Graduate School of Science and Technology Kumamoto University Kumamoto Japan Kumamoto Health Science University Kumamoto Japan

By visualizing DNA with diamidino phenylindole (DAPI), we found that hypothermal incubation followed by rewarming of human neutrophils resulted in an increased number of DAPI-positive objects representative of extensive DNA unfolding seemingly similar to neutrophil extracellular traps (NETs). In contrast to canonical NET formation, diphenylene iodonium (DPI), an NADPH oxidase inhibitor, exhibited negligible effects on formation of the DAPI-positive objects. Moreover, multiple instances of DNA damage were detected in the objects, but not in canonical NETs. Our results thus suggest the potential of hypothermia for triggering DNA structural alteration in neutrophils, which is similar to but distinct from NET formation.

关键词： Hypothermal Treatment DNA Unfolding Neutrophil Extracellular Trap (NET)

Review of heavy-ion inertial fusion physics

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Matter and Radiation at Extremes 2016年第2期1卷 89-113页

作者： S.kawata T.Karino A.I.Ogoyski Graduate School of Engineering Utsunomiya UniversityYohtoh 7-1-2Utsunomiya321-8585Japan CORE(Center for Optical Research and Education) Utsunomiya UniversityYohtoh 7-1-2Utsunomiya321-8585Japan Department of Physics Technical University of VarnaUlitskaStudentska 1VarnaBulgaria

In this review paper on heavy ion inertial fusion(HIF),the state-of-the-art scientific results are presented and discussed on the HIF physics,including physics of the heavy ion beam(HIB)transport in a fusion reactor,the HIBs-ion illumination on a direct-drive fuel target,the fuel target physics,the uniformity of the HIF target implosion,the smoothing mechanisms of the target implosion non-uniformity and the robust target *** HIB has remarkable preferable features to release the fusion energy in inertial fusion:in particle accelerators HIBs are generated with a high driver efficiency of~30%-40%,and the HIB ions deposit their energy inside of ***,a requirement for the fusion target energy gain is relatively low,that would be~50-70 to operate a HIF fusion reactor with the standard energy output of 1 GWof *** HIF reactor operation frequency would be~10-15 Hz or ***-MJ HIBs illuminate a fusion fuel target,and the fuel target is imploded to about a thousand times of the solid *** the DT fuel is ignited and *** HIB ion deposition range is defined by the HIB ions stopping length,which would be~1 mm or so depending on the ***,a relatively large density-scale length appears in the fuel target *** of the critical issues in inertial fusion would be a spherically uniform target compression,which would be degraded by a non-uniform *** implosion non-uniformity would be introduced by the Rayleigh-Taylor(R-T)instability,and the large densitygradient-scale length helps to reduce the R-T growth *** the other hand,the large scale length of the HIB ions stopping range suggests that the temperature at the energy deposition layer in a HIF target does not reach a very-high temperature:normally about 300 eV or so is realized in the energy absorption region,and that a direct-drive target would be appropriate in *** addition,the HIB accelerators are operated repetitively and *** precis

关键词： Heavy ion inertial fusion Heavy ion fusion reactor system Fusion fuel target implosion Implosion dynamics Heavy ion beam transport Rayleigh-Taylor instability stabilization Robust fusion system

Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line

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Cellular & Molecular Immunology 2021年第6期18卷 1545-1561页

作者： Hidetaka Seo Hitomi Masuda Kenjiro Asagoshi Tomoaki Uchiki Shigehisa kawata Goh Sasaki Takashi Yabuki Shunsuke Miyai Naoki Takahashi Shu-ichi Hashimoto Atsushi Sawada Aki Takaiwa Chika Koyama Kanako Tamai Kohei Kurosawa Ke-Yi Lin Kunihiro Ohta Yukoh Nakazaki Department of Life Sciences Graduate School of Arts and SciencesThe University of TokyoTokyoJapan Research Laboratories Chiome Bioscience IncTokyoJapan

Monoclonal antibodies(mAbs)are widely utilized as therapeutic drugs for various diseases,such as cancer,autoimmune diseases,and infectious *** the avian-derived B cell line DT40,we previously developed an antibody display technology,namely,the ADLib system,which rapidly generates antigen-specific ***,we report the development of a human version of the ADLib system and showcase the streamlined generation and optimization of functional human *** libraries were first constructed by replacing endogenous immunoglobulin genes with designed human *** these libraries,clones producing full-length human IgGs against distinct antigens can be isolated,as exemplified by the selection of antagonistic *** advantage of avian biology,effective affinity maturation was achieved in a straightforward manner by seamless diversification of the parental clones into secondary libraries followed by single-cell sorting,quickly affording mAbs with improved affinities and ***,we demonstrate that the human ADLib system could serve as an integrative platform with unique diversity for rapid de novo generation and optimization of therapeutic or diagnostic antibody ***,our results suggest that libraries can be constructed by introducing exogenous genes into DT40 cells,indicating that the ADLib system has the potential to be applied for the rapid and effective directed evolution and optimization of proteins in various fields beyond biomedicine.

关键词： Immunoglobulin rearrangements homologous recombination antibody therapeutics antibody engineering drug discovery

Pancreatic hyperechogenicity associated with hypoadiponectinemia and insulin resistance: A Japanese population study

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World Journal of Hepatology 2016年第33期8卷 1452-1458页

作者： Naohiko Makino Nakao Shirahata Teiichiro Honda Yoshiaki Ando Akiko Matsuda Yushi Ikeda Miho Ito Yuko Nishise Takafumi Saito Yoshiyuki Ueno Sumio kawata Department of Gastroenterology Faculty of MedicineYamagata University Department of Internal Medicine Hyogo Prefectural Nishinomiya Hospital

AIMTo examine the relationship between pancreatic hyperechogenicity and risk factors for metabolic syndrome. METHODSA general population-based survey of lifestyle-related diseases was conducted from 2005 to 2006 in Japan. The study involved 551 participants older than 40 year of age. Data for 472 non-diabetic adults were included in the analysis. The measures included the demographic factors, blood parameters, results of a 75 g oral glucose tolerance test, and abdominal ultrasonography. The echogenicity of the pancreas and liver was compared, and then the subjects were separated into two groups: cases with pancreatic hyperechogenicity (n = 208) and cases without (controls, n = 264). The differences between both groups were compared using an unpaired t-test or Fisher’s exact test. Multiple logistic regression analysis was used to determine the relationship between the pancreatic hyperechogenicity and clinical and biochemical parameters. RESULTSSubjects with pancreatic hyperechogenicity had decreased serum adiponectin concentration compared to control subjects [8.9 (6.5, 12.8) vs 11.1 (7.8, 15.9), P P CONCLUSIONPancreatic hyperechogenicity is independently associated with increased BMI, insulin resistance, and hypoadiponectinemia in the general population.

关键词： Pancreatic hyperechogenicity Metabolic syndrome Obesity Adiponectin The Takahata study

CONSTRUCTION OF A GENOMIC DNA LIBRARY WITH ATA VECTOR AND ITS APPLICATION IN CLONING OF THE PHYTOENE SYNTHASE GENE FROM THE CYANOBACTERIUM SPIRULINA PLATENSIS M-135

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Chinese Journal of Oceanology and Limnology 1998年第S1期16卷 17-24页

作者： Yoshikazu kawata Shin-ichi Yano Hiroyuki Kojima(Osaka Mational Research Institute. Agency of Industrial Science and Technology, Ikeda, Osaka 563, Japan)

An efficient and simple method for constructing a genomic DNA library using a TAcloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification offragment ends with Taq DNA polymerase, followed by ligation using a TA vector. This method was ap-plied for cloning of the phytoene synthase gene crtB from Spirulina platensis. This method is useful whengenomic DNA cannot be efficiently digested with restriction enzymes, a problem often encountered duringthe construction of a genomic DNA library of cyanobacteria.

关键词： genomic library Spirulina phytoene synthase carotenoid