CONSTRUCTION OF A GENOMIC DNA LIBRARY WITH ATA VECTOR AND ITS APPLICATION IN CLONING OF THE PHYTOENE SYNTHASE GENE FROM THE CYANOBACTERIUM SPIRULINA PLATENSIS M-135

作     者：Yoshikazu Kawata Shin-ichi Yano Hiroyuki Kojima(Osaka Mational Research Institute. Agency of Industrial Science and Technology, Ikeda, Osaka 563, Japan) 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：1998年第16卷第S1期

页      面：17-24页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：genomic library Spirulina phytoene synthase carotenoid 

摘      要：An efficient and simple method for constructing a genomic DNA library using a TAcloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification offragment ends with Taq DNA polymerase, followed by ligation using a TA vector. This method was ap-plied for cloning of the phytoene synthase gene crtB from Spirulina platensis. This method is useful whengenomic DNA cannot be efficiently digested with restriction enzymes, a problem often encountered duringthe construction of a genomic DNA library of cyanobacteria.