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Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of Escherichia coliATCC 25922 Surrogated for E. coliO157:H7

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Journal of Biomaterials and Nanobiotechnology 2013年第4期4卷 10-19页

作者： Miki kubo Yori Ohshima Fumio Irie Mikio Kikuchi Jun Sawai Faculty of Applied Bioscience Kanagawa Institute of Kanagawa Atsugi Japan

The ability of heated scallop-shell powder (HSSP) to disinfect Escherichia coli ATCC 25922 biofilm was investigated. On account of its cryotolerance and cell surface characteristics, the E. coli strain is reportedly a useful surrogate for E. coli O157: H7 in surface attachment studies. In this study, an E. coli ATCC 25922 biofilm was formed on a glass plate, and immersed in a slurry of HSSP. Following treatment, the disinfection ability of the HSSP toward the biofilm was non-destructively and quantitatively measured by conductimetric assay. The disinfection efficacy increased with HSSP concentration and treatment time. HSSP treatment (10 mg/mL, pH 12.5) for 20 min completely eliminated biofilm bioactivity (approximately 108 CFU/cm2 in non-treated biofilms). In contrast, treatment with NaOH solution at the same pH, and treatment with sodium hypochlorite (200 mg/mL) reduced the activity by approximately one to three log10. Fluorescence microscopy confirmed that no viable cells remained on the plate following HSSP treatment (10 mg/mL). Although alkaline and sodium hypochlorite treatments removed cells from the biofilm, under these treatments, many viable cells remained on the plate. To elucidate the mechanism of HSSP activity against E. coli ATCC 25922, the active oxygen generated from the HSSP slurry was examined by chemiluminescence analysis. The luminescence intensity increased with increasing concentration of HSSP slurry. The results suggested that, besides being alkaline, HSSP generates active oxygen species with sporicidal activity. Thus, HSSP treatment could also be effective for controlling biofilms of the toxic strain E. coli O157: H7, implicated in food poisoning.

关键词： Scallop-Shell Powder Biofilm Escherichia coli Conductimetric Assay Fluorescence Microscopy Active Oxygen Alkali

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Depressive mood, bonding failure, and abusive parenting among mothers with three-month-old babies in a Japanese community

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Open Journal of Psychiatry 2013年第3期3卷 1-7页

作者： Toshinori Kitamura Yukiko Ohashi Sachiko Kita Megumi Haruna Reiko kubo Department of Midwifery and Women’s Health University of Tokyo Tokyo Japan Kitamura Institute of Mental Health Tokyo Japan Kumamoto Prefecture Kumamoto Japan

Objective: To investigate the relationships between depressive mood, bonding failure, and abusive parenting. Method: We distributed questionnaires to 1198 mothers attending a three-month postnatal health check-up in rural areas in Japan. The questionnaires assessed these three variables along with demographics. We evaluated the causal relationships by comparing different structural equation models to the data. Results: Although all the models fit the data well, the best Akaike Information Criterion was obtained from a model where both depressive mood and bonding failure predict abusive parenting, but depressive mood and bonding failure do not predict each other directly. The determinant coefficient of child abuse in the final model was 0.13. Discussion: We found depressive mood and bonding failure during postpartum period impacted negatively on mother’s parenting behaviour. Because only 13% of the variance of abusive parenting was explainable by this model, further study should be needed to identify other risk factors of child abuse. Similarly, midwives and public health nurse in community should pay attention to not only depression but also bonding failure to prevent child abuse.

关键词： Postnatal Depression Bonding Failure Child Abuse Mothers of Neonates Structural Equation Analysis

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Analysis of the Alkane Hydroxylase Gene and Long-Chain Cyclic Alkane Degradation in Rhodococcus

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Advances in Microbiology 2019年第3期9卷 151-163页

作者： Taiki Kawagoe Kenzo kubota Kiwako S. Araki Motoki kubo Department of Biotechnology Faculty of Life Sciences Ritsumeikan University Shiga Japan

To characterize the long-chain cyclic alkane (c-alkane) degradation of bacteria in Rhodococcus, we analyzed the relationship between the alkane hydroxylase gene (alkB) and long-chain c-alkane degradation in 19 species. Eleven strains which were isolated from nature using long-chain c-alkane as a substrate were identified as R. erythropolisc, and all were shown to carry the alkB [alkB R2 type]. This gene type was also carried by two other species, R. rhodochrous and R. baikonurensis. In total, 17 species of the genus Rhodococcus carried alkB, but the gene types differed from each other. The two species R. rhodnii and R. coprophilus did not carry alkB, and their long-chain c-alkane degradation levels were low.

关键词： Alkane Hydroxylase alkB Bioremediation Long-Chain c-Alkane Rhodococcus

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The Concept Study of Recombinant Human Soluble Thrombomodulin in Patients with Acute Respiratory Distress Syndrome

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International Journal of Clinical Medicine 2013年第11期4卷 488-495页

作者： Kenji Tsushima Toshiki Yokoyama Tomonobu Koizumi Keishi kubo Koichiro Tatsumi Department of Pulmonary Medicine Shinonoi General Hospital Nagano Japan Department of Respirology Graduate School of Medicine Chiba Uni-versity Chiba Japan Department of Respirology Graduate School of Medicine Chiba Uni-versity Chiba Japan First Department of Internal Medicine Shinshu University School of Medicine Nagano Japan

Background: Recombinant human soluble thrombomodulin (rhTM) was approved for the treatment of disseminated intravascular coagulation in Japan, and rhTM has anti-inflammatory effects. Disordered coagulation is a part of the acute respiratory distress syndrome (ARDS) pathophysiology and thus we hypothesize that anticoagulant therapy may help. This preliminary study was to observe the safety of rhTM administration and the improvement on biomarker levels after the therapy for ARDS-patients. Objectives: Case series of ARDS-patients. Methods: Seventeen ARDS-patients that required ventilatory management were treated with rhTM and clinical and laboratory data were collected including platelets, thrombin-antithrombin complex (TAT), fibrinogen degradation products, oxygen saturation/the fraction of inspired oxygen (SpO2/FIO2), and high-mobility group-1 (HMG-1). The administration of rhTM was started during 6 days at a bolus dose of 0.06 mg/kg/day immediately after the diagnosis of ARDS. Results: Eleven of the 17 ARDS-patients were alive at 28 days after the beginning of the administration of rhTM. The serial pattern of the SpO2/FIO2 showed remarkable differences between the survivors and nonsurvivors from day 5 to day 7. The TAT in the survivors significantly decreased after treatment, and there were significantly lower levels in the TAT on day 7 in comparison to that of the nonsurvivors. The serial changes of HMG-1 showed increased levels in the nonsurvivors until day 5 after the administration of rhTM. Conclusions: Additional rhTM administration can safely improve the parameters in survival ARDS-patients, as demonstrated by significant improvements in the SpO2/FIO2, HMG-1 and TAT.

关键词： Acute Respiratory Distress Syndrome Recombinant Human Soluble Thrombomodulin Thrombin-Antithrombin Complex SpO2/FIO2 High-Mobility Group-1

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Chlorella Residue Functions as a Bio-Stimulant to Promote Plant Growth and Improve Soil Fertility

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Journal of Agricultural Chemistry and Environment 2024年第4期13卷 373-383页

作者： Asuka Okazaki Toru Mizoguchi Zakirul Islam Quoc Thinh Tran Motoki kubo Laboratory of Bioengineering Department of Biotechnology Graduate School of Advanced Life Sciences Ritsumeikan University Shiga Japan Production and Development Department Sun Chlorella Co. Ltd. Kyoto Japan

Chlorella residues are currently underutilized. Therefore, in this study, we analyzed the nutritional components of Chlorella residue, and investigated its potential use as an organic fertilizer/bio-stimulant. Composition analyses revealed that the Chlorella residue contained a substantial amount of nitrogen (97,910 mg/kg), and significant quantities of secondary macronutrients, such as calcium (4300 mg/kg) and magnesium (9700 mg/kg), and micronutrients, such as iron (1850 mg/L) and manganese (359 mg/kg). The application of Chlorella residue to soil resulted in increased soil bacterial biomass. When Chlorella residue was added to the soil at a rate of 0.5% or 1.0% (w/w), the fresh weights of Brassica rapa and Spinacia oleracea were significantly increased. Furthermore, the application of Chlorella residue to the soil of B. rapa suppressed the reduction of the microbiome caused by clubroot disease and decreased the clubroot disease index. Therefore, Chlorella residue can be included in organic fertilizers that effectively improve soil nutrient contents, promote plant growth, and reduce the incidence of disease.

关键词： Bacterial Biomass Bacterial Diversity Chlorella Residue Clubroot Disease Plant Disease

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Comparison of clinicopathological characteristics between resected ampullary carcinoma and carcinoma of the second portion of the duodenum

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World Journal of Gastrointestinal Surgery 2022年第11期14卷 1219-1229页

作者： Kohei Nishio Kenjiro Kimura Akihiro Murata Go Ohira Hiroji Shinkawa Shintaro Kodai Ryosuke Amano Shogo Tanaka Sadatoshi Shimizu Shigekazu Takemura Akishige Kanazawa Shoji kubo Takeaki Ishizawa Department of Hepato-Biliary-Pancreatic Surgery Osaka Metropolitan UniversityOsaka 5458585Japan Department of HepatoBiliary-Pancreatic Surgery Osaka City General HospitalOsaka 5340021Japan

BACKGROUND Few studies compared the oncological and biological characteristics between ampullary carcinoma(AC) and cancer of the second portion of the duodenum(DC-Ⅱ), although both tumors arise from anatomically close *** To elucidate differences in clinicopathological characteristics, especially the patterns of lymph node metastasis(LNM), between AC and DC-Ⅱ.METHODS This was a retrospective cohort study of 80 patients with AC and 27 patients with DC-Ⅱ who underwent pancreaticoduodenectomy between January 1998 and December 2018 in two institutions. Clinicopathological factors, LNM patterns, and prognosis were compared between the two *** The patients with AC and DC-Ⅱ did not exhibit significant differences in 5-year overall survival(66.0% and 67.1%, respectively) and 5-year relapse-free survival(63.5% and 62.2%, respectively). Compared to the patients with DC-Ⅱ, the rate of preoperative biliary drainage was higher(P = 0.042) and the rates of digestive symptoms(P = 0.0158), ulcerative-type cancer(P < 0.0001), large tumor diameter(P < 0.0001), and advanced tumor stage(P = 0.0019) were lower in the patients with AC. The LNM rates were 27.5% and 40.7% in patients with AC and DC-Ⅱ,respectively, without significant difference(P = 0.23). The rates of LNM to hepatic nodes(N-He)and pyloric nodes(N-Py) were significantly higher in patients with DC-Ⅱ than in those with AC(metastasis to N-HE: 18.5% and 5% in patients with DC-Ⅱ and AC, respectively;P = 0.0432;metastasis to N-Py: 11.1% and 0% in patients with DC-Ⅱ and AC, respectively;P = 0.0186)CONCLUSION Although there were no significant differences in the prognosis and recurrence rates between the two groups, metastases to N-He and N-Py were more frequent in patients with DC-Ⅱ than in those with AC.

关键词： Ampulla of Vater Duodenum Lymphatic metastasis pattern Lymphatic metastasis station Lymph node excision Neoplasm Pancreaticoduodenectomy

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Analysis of Chemical and Biological Soil Properties in Organically and Conventionally Fertilized Apple Orchards

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Journal of Agricultural Chemistry and Environment 2016年第2期5卷 92-99页

作者： Takamitsu Kai Masaki Mukai Kiwako S. Araki Dinesh Adhikari Motoki kubo Department of Biotechnology Faculty of Life Sciences Ritsumeikan University Kusatsu Japan

We compared chemical and biological properties of soils in organically and conventionally fertilized apple orchards in Nagano Prefecture (one of the major apple producing regions in Japan). Five apple orchards with different fertilizer management systems were used for this study. The total carbon and total nitrogen contents were higher in the organically fertilized orchard, while the total phosphorus and total potassium were at similar levels in both organically and conventionally fertilized orchards. The bacterial biomass did not differ between the two orchards, but the N circulation activity was clearly higher in the organically fertilized orchard from April to December. Total carbon from 50,000 to 60,000 mg/kg, total nitrogen at about 3000 to 4000 mg/kg, and a C/N ratio of 15 - 20 were suggested to be suitable conditions for a high level of apple production under an organic fertilizer management system.

关键词： Component Formatting Style Styling Insert Organic Fertilizer Management System Apple Bacterial Biomass Nitrogen Circulation Activity

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Short-Wave Infrared PbS Colloidal Quantum Dot/ZnO Nanowire Solar Cells

Short-Wave Infrared PbS Colloidal Quantum Dot/ZnO Nanowire S...

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第五届新型太阳能电池学术研讨会

作者： Haibin Wang Takaya kubo Jotara Nakazaki Hiroshi Segawa Research Center for Advanced Science and Technology The University of Tokyo

Colloidal quantum dots(CQDs) give unique optical properties such as size dependent absorption,which makes the quantum dot promising candidate materials toward high efficiency solar *** most widely studied PbS CQD solar structures are based on depleted heterojunction that is formed by deposition of CQD layers on top of an electron-accepting layer such as ZnO or Ti *** enhance the performance of the solar cells,several strategies,which include new solar cell architectures and bandgap engineering,were reported.1 We focused on a solar cell composed of PbS QD/ZnO nanowire(NW) structures(Fig.1) with the aim of achieving efficient carrier transport and light absorption in the near-infrared region simultaneously.2,3 These PbS QD-based solar cells were also found to have high stability under continuous light soaking.4 We investigated the performance of PbS QD/ZnO NW solar cells using different PbS CQDs that absorb photons in a wide range of the solar spectrum.5 The solar cells were confirmed to convert a wide range of solar energy(0.35-2.0μm)(Fig.1).We found that the solar cells working in short-wave infrared(SWIR)region had a high open-circuit voltage(Voc) and the relatively low incident photon energy loss and small Voc deficit(Fig.2).These results indicate that solution-processed PbS CQD solar cells with ZnO nanowires are promising candidates for the middle and bottom subcells of multi-junction solar cells.5

关键词： Short-Wave Infrared PbS Quantum Dots ZnO Nanowire Solar Cells

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Quantification of the Vertical Load Applied to the Pavement during Cornering Maneuver of a Battery Electric Commercial Vehicle

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Journal of Traffic and Transportation Engineering 2024年第5期12卷 207-213页

作者： Pablo kubo Ney Siqueira Gilberto Grossl Luis Welin Mairon Dobicz Marcio Andrade Department of Complete Vehicle Volvo Group North AmericaGreensboro 27409USA Department of Complete Vehicle Volvo Trucks BrazilCuritiba 81260-900Brazil

The wheel loads of heavy trucks are the major source of pavement damage,given the repeated loadings imposed by them due to transient events and surface *** related studies focus on steady-state context regarding simplified vehicle’s parameters and ideal pavement conditions,this paper aims to analyze the vertical load applied to the pavement by considering cornering maneuver as a transient event,on a battery electric vehicle *** this concern,measurements were performed on a rigid truck,with two steering front axles,in a closed course proving *** relationship has been presented between vehicle’s speed,lateral acceleration and transferred vertical load for a given curve radius of 85.6 m and 3.7°of transversal *** measurements results indicated that for every 10 km/h increasing on the vehicle’s speed,additional 110 kgf will be transferred to the pavement on the outer side of the cornering *** value itself could not be considered high,but it will be also added to the static load,or overload in some truck applications.

关键词： Load transfer durability vehicle dynamics

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High efficiency infrared PbS quantum dot solar cells toward the bottom subcell of tandem solar cells

High efficiency infrared PbS quantum dot solar cells toward ...

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第六届新型太阳能电池材料科学与技术学术研讨会

作者： Haibin Wang Takaya kubo Jotara Nakazaki Heroshi Segawa RCAST The University of Tokyo

Development of ultra-high efficiency low cost tandem solar cell is required for efficient utilization of solar energy. For the purpose, PbS colloidal quantum dots(CQDs) have been gaining much attention as promising constituent materials for solution processed-infrared solar cells because PbS QDs can absorb photon energy in a wider range of the solar spectrum. Among various CQD-based solar cells, PbS QD/ZnO-based depleted heterojunction solar cells showed a power conversion efficiency(PCE) over 12 % in 2018.1 But there is a limited number of literatures reporting on PbS QD based solar working in low photon energy region(< 1.0 eV). Our recent study revealed that PbS QD/ZnO nanowire(NW) solar cells were able to convert a wide range of solar energy to electricity. However, the free carrier absorption of the transparent conductive oxide(FTO: F-doped SnO) in infrared region cause a non-negligible energy loss of the incident solar energy to be absorbed by the PbS QD layer. In this presentation, we constructed infrared PbS QD/ZnO NW solar cells having the first exciton absorption peak at 1560 nm(0.8 eV) and successfully improved the spectral sensitivity in infrared region by employing highly infrared transparent 1% Ta-doped SnO(TTO) electrodes. The EQE reached its maximum value of 47% at 1560 nm. The integrated photo-current density obtained from the EQE spectrum over 38 mAcm for full solar spectrum(Fig.1). The solar cell installed with an 870 nm sharp-cut filter yielded a PCE of 2.02 %(J=14.8 mAcm;Vc=0.285 V;FF=47.9 %) under a filtered one-sun illumination. The PCE is the highest efficiency reported to date for solution processed solar cells working in the infrared region. Based on these results, we will discuss the potential of PbS QD/ZnO NW solar cells toward the bottom subcells of tandem solar cells.

关键词： Lead(Ⅱ) sulfide Colloidal quantum dots ZnO Nanowires Infrared

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