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In vivo Studies on the Roles of Tic55-Related Proteins in Chloroplast Protein Import in Arabidopsis thaliana

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Molecular Plant 2009年第6期2卷 1397-1409页

作者： Patrik Boij Ramesh Patel Christel Garcia Paul Jarvis henrik Aronsson Department of Plant and Environmental Sciences University of Gothenburg Box 461 SE-405 30 Gothenburg Sweden Department of Biology University of Leicester University Road Leicester LE1 7RH UK

The TicS5 （Translocon at the inner envelope membrane of chloroplasts, 55 kDa） protein was identified in pea as a putative regulator, possibly linking chloroplast protein import to the redox state of the photosynthetic machinery. Two Tic55 homologs have been proposed to exist in Arabidopsis： atTic55-11 and AtPTC52 （Protochlorophyllide-dependent Trans- Iocon Component, 52 kDa; has also been called atTic55-1V）. Our phylogenetic analysis shows that attic55-11 is an ortholog of psTic55 from pea （Pisum sativurn）, and that AtPTC52 is a more distant homolog of the two. AtPTC52 was included in this study to rule out possible functional links between the proteins in Arabidopsis. No detectable mutant phenotypes were found in two independent T-DNA knockout mutant plant lines for each Arabidopsis protein, when compared with wild- type： visible appearance, chlorophyll content, photosynthetic performance, and chloroplast protein import, for example, were all normal. Both wild-type and tic55-11 mutant chloroplasts exhibited deficient protein import when treated with diethylpyrocarbonate, indicating that Tic55 is not the sole target of this reagent in relation to protein import. Furthermore, ptc52 mutant chloroplasts were not defective with respect to pPORA import, which was previously reported to involve PTC52 in barley. Thus, we conclude that atTic55-11 and AtPTC52 are not strictly required for functional protein import in Arabidopsis.

关键词： Arabidopsis chloroplast import protein targeting Tic55.

Partial gastric pull-up in the treatment of patients with long-gap esophageal atresia

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World Journal of Pediatrics 2015年第3期11卷 267-271页

作者： Marc Reismann Tina Granholm henrik Ehrén Department of Pediatric Surgery and Urology Astrid Lindgren Children's HospitalKarolinska University HospitalStockholmSweden

Background:This study was to analyze outcomes of long-gap esophageal atresia(lGEA)treated with partial gastric pull-up(PGP)into the ***:The medical records of all children who had undergone PGP for lGEA from 1999 to 2012 were *** data,initial postoperative course,complications,time to full oral nutrition,follow-up diagnostics and nutritional status were ***:Nine children who had undergone PGP were followed up for a mean period of 6.2±3.1 *** median gestational age was 37+2 weeks,and mean birth weight 2462±658 *** children were primarily treated with a gastrostomy,their mean age at PGP was 11.4+10.9 weeks and mean weight was 4484+1966 *** mean operation time was 199+51 *** was an early postoperative complication in three children,one of whom had a consecutive stricture *** complications were stenosis(n=7)and gastro-esophageal reflux(n=5).The general status of the children was judged as"good"or"very good"on the last *** median percentile of the body-mass-index was *** at 3.7±3.2 years after the operation revealed a grade I esophagitis in two *** was no death in this group of ***:Because of its high complication rate,partial gastric pull-up cannot be recommended as an alternative for the treatment of lGEA at present.A final judgment could be made on the basis of a comparative study.

关键词： early childhood esophageal atresia gastric pull-up

Oxidation controlled lift-off of 3D chiral plasmonic Au nano-hooks

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Nano Research 2019年第7期12卷 1635-1642页

作者： Gunnar Klos Amanda Andersen Matteo Miola henrik Birkedal Duncan S. Sutherland Interdisciplinary Nanoscience Center (iNANO) Aarhus UniversityAarhus C 8000Denmark Department of Chemistry and iNANO Aarhus UniversityAarhus C 8000Denmark Carbon Dioxide Activation Center (CADIAC)- iNANO Aarhus UniversityAarhus C 8000Denmark

Colloidal suspensions of plasmonic nanoparticles (NPs) are a well-established tool for biomedical applications and enhanced spectroscopy because of their strong optical *** specific response is greatly dependent on the NP *** strong optical activity of chiral NPs has created special interest but fabrication of chiral NPs in solution remains ***,we present an approach whereby three-dimensional (3D) chiral Au nano-hooks,fabricated with the parallel hole-mask colloidal lithography (HMCl) method,can be lifted off from a glass substrate in a controllable manner by using a combined treatment with oxygen plasma oxidation and a reduction step in *** method has the advantage of being based on established techniques and not requiring strong acids or complex substrates as in etching based *** furthermore demonstrate the integration of the hook NPs into reversibly cross-linked hydrogels inspired by mussel catechol chemistry but containing an oxidation resistant catechol analogue grafted onto poly(allylamine) crosslinked by coordination of Al^3+ and how this facilitates the remote analysis of hydrogel microenvironment,*** water *** suspended particles are promising candidates for optically active surface-enhanced Raman spectroscopy (SERS),asymmetric photo catalysis or aggregation *** integration into hydrogels to produce functional hydrogels holds benefits for applications of metamaterials in optics,sensing or activation in environmental remediation or drug delivery.

关键词： chiral nanostructures circular dichroism plasmonic sensor hole-mask colloidal lithography

An introduction to multivariate probabilistic forecast evaluation

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能源与人工智能(英文) 2021年第2期4卷 119-135页

作者： Mathias Blicher Bjerregard Jan Kloppenborg Møller henrik Madsen Technical University of Denmark Department of Applied Mathematics and Computer Denmark

Probabilistic forecasting is becoming increasingly important for a wide range of applications,especially for en-ergy systems such as forecasting wind power production.A need for proper evaluation of probabilistic forecasts follows naturally with this,because evaluation is the key to improving the *** plenty of excellent reviews and research papers on probabilistic forecast evaluation already exist,we find that there is a need for an introduction with some practical *** particular,many forecast scenarios in energy systems are inher-ently multivariate,and while univariate evaluation methods are well understood and documented,only limited and scattered work has been done on their multivariate *** paper therefore contains a review of a selected set of probabilistic forecast evaluation methods,primarily scoring rules,as well as practical sections that explain how these methods can be calculated and *** three case studies featuring simple autore-gressive models,stochastic differential equations and real wind power data,we implement,apply and discuss the logarithmic score,the continuous ranked probability score and the variogram score for forecasting problems of varying ***,the advantages and disadvantages of the three scoring rules are highlighted,and this provides a significant step towards deciding on an evaluation method for a given multivariate forecast scenario including forecast scenarios relevant for energy systems.

关键词： Probabilistic forecast evaluation Multivariate scoring rules Wind power forecast Ensemble forecast Time series analysis

Identification of non-linear autoregressive models with exogenous inputs for room air temperature modelling

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能源与人工智能(英文) 2022年第3期9卷 78-87页

作者： Christian Ankerstjerne Thilker Peder Bacher Davide Cali henrik Madsen Technical University of Denmark Department of Applied Mathematics and Computer ScienceAsmussens AlléBuilding 303BDK-2800 Kgs.LyngbyDenmark

This paper proposes non-linear autoregressive models with exogenous inputs to model the air temperature ineach room of a Danish school building connected to the local district heating network. To obtain satisfactorymodels, the authors find it necessary to estimate the solar radiation effect as a function of the time of the dayusing a B-spline basis expansion. Furthermore, this paper proposes a method for estimating the valve positionof the radiator thermostats in each room using modified Hermite polynomials to ensure monotonicity of theestimated curve. The non-linearities require a modification in the estimation procedure: Some parametersare estimated in an outer optimisation, while the usual regression parameters are estimated in an inneroptimisation. The models are able to simulate the temperature 24 h ahead with a root-mean-square-errorof the predictions between 0.25℃ and 0.6℃. The models seem to capture the solar radiation gain in away aligned with expectations. The estimated thermostatic valve functions also seem to capture the importantvariations of the individual room heat inputs.

关键词： Time series analysis Non-linear models District heating Smart energy systems

The potential role of associating liver partition and portal vein ligation in the treatment of colorectal liver metastases

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Hepatobiliary Surgery and Nutrition 2021年第6期10卷 902-905页

作者： Jan P.Jonas henrik Petrowsky Swiss HPB and Transplantation Center Department of SurgeryUniversity Hospital ZurichZurichSwitzerland HPB Center Department of SurgeryClinic FavoritenViennaAustria

life expectancy of patients with colorectal liver metastasis(CRlM)significantly improved over the last decades due to the induction of multimodal effective treatment strategies including perioperative chemotherapy and advanced surgical *** conversion chemotherapy,previously unresectable CRlMs can be converted to a resectable situation and amenable to procedures such as associating liver partition and portal vein ligation(AlPPS)(1).A recent publication in HBSN has extensively reviewed AlPPS as a concept in the treatment of *** review article by Glinka et al.(2)focuses on the evolvement of this novel procedure and describes in detail the gradual changes accompanying this procedure’s evolvement since ***,the authors highlight several aspects crucial for the best possible outcome after this procedure including patient selection,future liver remnant,technical aspects,safety,morbidity,mortality and oncological results.

关键词： chemotherapy colorectal treatment

An arctic low-energy house as experimental setup for studies of heat dynamics of buildings

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Frontiers of Architectural Research 2013年第4期2卷 488-499页

作者： Philip Delft Andersen Carsten Rode henrik Madsen DTU Compute Richard Pedersens Plads Technical University of Denmark Building 322 Kongens Lyngyb DK-2800 Denmark DTU Civil Engineering Brovej Bygning 118 Technical University of Denmark Kongens Lyngyb DK-2800 Denmark

This paper addresses the difficulties in pinpointing reasons for unexpectedly high energy consumption in construction, and in low-energy houses especially. Statistical methods are applied to improve the insight into the energy performance and heat dynamics of a building based on consumption records and weather data. Dynamical methods separate influences from outdoor temperature, solar radiation, and wind on the energy consumption in the building. The studied building is a low-energy house in Sisimiut, Greenland. Weather conditions like large temperature differences between indoors and outdoors throughout tong winters, strong winds, and very different circumstances regarding solar radiation compared to areas where low-energy houses are usually built, make the location very interesting for modeling and testing purposes. In 2011 new measurement equipment was installed in the house, which will be used to develop more detailed models of the heat dynamics and energy performance in relation to different meteorological variables, heating systems, and user behavior. This type of models is known as a graybox model and is been introduced in this paper.

关键词： low-energy houses Heat dynamics Arctic climate Statistical modeling Graybox modeling

Finite-time consensus protocols for multi-dimensional multi-agent systems

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Control Theory and Technology 2020年第4期18卷 419-430页

作者： Jieqiang Wei Bart Besselink Junfeng Wu henrik Sandberg Karl H.Johansson Ericsson Global AI Accelerator Torshamnsgatan 21StockholmSweden Bernoulli Institute for Mathematics Computer Science and Artificial IntelligenceUniversity of GroningenP.O.Box 4079700 GroningenAKThe Netherlands College of Control Science and Engineering Zhejiang University310027 HangzhouChina Division of Decision and Control Systems KTH Royal Institute of Technology10044 StockholmSweden

A fnite.-time consensus protocol is proposed for multi -dimensional multi- agent systems, using direction peserving signumcontrols. Flipp solutions and nonsmooh analysis tehniques are adopted to handle discontinuities. Suficient and ncessaryconditions are provided to guarantee infinte time convergence and boundedness of the solutions. It turns out that the numberof agents which have cotinuous contol law plays an ssenan role in fnite-tine conerence In adidio it is shown thatthe unit bals itoduced bylp, norms. where p ∈[1,∞] , are inariat for the closed lop.

关键词： Multi-agent systems Network consensus Finite time convergence Nonsmooth analysis

Effects of a novel bacterial phytase expressed in Aspergillus Oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs

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Journal of Animal Science and Biotechnology 2013年第2期4卷 102-111页

作者： Ferdinando Nielsen Almeida Rommel Casilda Sulabo Hans henrik Stein Department of Animal Sciences University of Illinois

In 2 experiments, 48 weanling （initial BW： 13.5 ± 2.4 kg, Exp. 1） were used to determine effects of a novel bacterial 6-phytase and 24 growing pigs （initial BW： 36.2 ± 4.0 kg, Exp. 2） expressed in Aspergillus oryzae on the apparent total tract digestibility （ATTD） of phosphorus and calcium in corn-soybean meal diets fed to weanling and growing pigs. In Exp. 1 and 2, pigs were randomly allotted to 6 dietary treatments using a randomized complete block design and a balanced 2 period changeover design, respectively. In both experiments, 6 diets were formulated. The positive control diet was a corn-soybean meal diet with added inorganic phosphorus （Exp. 1：0.42 and 0.86% standardized total tract digestible phosphorus and total calcium, respectively; Exp. 2：0.32 and 0.79% standardized total tract digestible phosphorus and total calcium, respectively）. A negative control diet and 4 diets with the novel phytase （Ronozyme HiPhos, DSM Nutritional Products Inc., Parsippany, N J） added to the negative control diet at levels of 500, 1,000, 2,000, and 4,000 phytase units （FYT）/kg were also formulated. In Exp. 1, the ATTD of phosphorus was greater （P 〈 0.01） for the positive control diet （60.5%） than for the negative control diet （40.5%）, but increased （linear and quadratic, P 〈 0.01） as phytase was added to the negative control diet （40.5% vs. 61.6%, 65.1%, 68.7%, and 68.0%）. The breakpoint for the ATTD of phosphorus （68.4%） was reached at a phytase inclusion level of 1,016 FYT/kg. In Exp. 2, the ATTD of phosphorus was greater （P〈 0.01） for the positive control diet （59.4%） than for the negative control diet （39.8%） and increased （linear and quadratic, P〈 0.01） as phytase was added to the negative control diet （39.8% vs. 58.1%, 65.4%, 69.1%, and 72.8%）. The breakpoint for the ATTD of phosphorus （69.1%） was reached at a phytase inclusion level of 801 FYT/kg. in conclusion, the novel bacterial 6-phytase improved the ATTD of phosphorus and calcium in both weanling and growing pigs.

关键词： Calcium Digestibility Phosphorus Phytase Pigs