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欧洲中部刺槐种源群体等位酶变异研究

Acta Genetica Sinica 2004年第12期31卷 1439-1447页

作者：杨敏生 heike Hertel Volker Schneck 河北农业大学 Institute for Forest Genetics and Forest Tree Breeding Federal Research Centre for Forestry and Forest ProductsWaldsivers dorfD15377Germany

从欧洲中部及美国收集了 18个刺槐种源种子 ,以 2年生苗木为材料 ,采用水平切片淀粉凝胶电泳和聚丙烯酰胺凝胶电泳对 11个酶系统进行了检测 ,共发现 2 0个酶位点 ,其中 14个为多态位点 ,多态性位点百分数为70 %。在参加计算的 7个酶系统... 详细信息

从欧洲中部及美国收集了 18个刺槐种源种子 ,以 2年生苗木为材料 ,采用水平切片淀粉凝胶电泳和聚丙烯酰胺凝胶电泳对 11个酶系统进行了检测 ,共发现 2 0个酶位点 ,其中 14个为多态位点 ,多态性位点百分数为70 %。在参加计算的 7个酶系统的 12个多态性位点中 ,平均每个位点的等位基因数 (A)和平均每位点等位基因有效数 (Ae)为 2 733和 1.794 ,平均每个位点的实际杂合度 (Ho)和预期杂合度 (He)为 0 36 8和 0 4 0 0 ,固定指数 (F)为 0 0 80 ,绝大多数位点固定指数为正值。各基因位点遗传参数与 12个位点平均值进行相关分析表明 ,Fe b和Lap a的大部分群体遗传参数 (A、Ae、Ho、He等 )与平均值存在紧密的相关关系 ,其他位点各项遗传参数和总平均值相关不明显 ,这些位点的重要性可能比相关性较好的位点更大。德国 8个种源群体间遗传距离变化在 0 0 9～0 2 6之间 ,来自匈牙利的 6个种源之间遗传距离很小 ,绝大部分均在 0 11以下 ;德国的 8个种源与匈牙利和斯洛伐克的 8个种源之间的遗传距离变化在 0 0 9～ 0 2 4之间 ;来自美国的 2个种源 ,与欧洲的种源间的遗传距离在0 0 9～ 0 2 3之间 ,这一距离没有超出欧洲种源之间的差异。匈牙利和斯洛伐克的 8个种源群体的各项遗传参数均高于德国种源 ,表明这?

关键词：等位酶遗传多样性刺槐种源群体

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Schlafhorst 捻接技术的模块化

纺织导报 2005年第10期 143-144页

作者： heike Scheibe

通过Autoconer 338产品系列,Schlafhorst(赐来福)公司向纺织行业提供了能够高效率卷绕各种规格纱线卷装的成熟、先进技术.而灵活、创新的模块技术为纱线自动络筒工艺提高经济效益奠定了基础.络筒机的主要工作之一是当纱线出现断头,或换... 详细信息

通过Autoconer 338产品系列,Schlafhorst(赐来福)公司向纺织行业提供了能够高效率卷绕各种规格纱线卷装的成熟、先进技术.而灵活、创新的模块技术为纱线自动络筒工艺提高经济效益奠定了基础.络筒机的主要工作之一是当纱线出现断头,或换纱管,或清纱之后,将两个纱头接在一起,形成一个几乎与正常纱线无区别的接头.这是Schlafhorst公司研发工作的重点之一,因此,该公司一直致力于对纱线捻接工艺的深入研究.

关键词： Schlafhorst公司络筒机捻接技术模块化设计自动捻接器

Spatio-temporal changes of exposure and vulnerability to floods in China

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Advances in Climate Change Research 2014年第4期5卷 197-205页

作者： WANG Yan-Jun GAO Chao ZHAI Jian-Qing LI Xiu-Cang SU Bu-da HARTMANN heike School of Geography and Remote Sensing Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University ofInformation Science & Technology College of Territorial Resources and Tourism Anhui Normal University National Climate Center China Meteorological Administration Department of Geography Geologyand the EnvironmentSlippery Rock University of Pennsylvania

A socio-economic data set on China＇s historical flood losses for the period 1984--2012 was compiled to analyze the exposed population, economy, and crop area as well as the vulnerabilities of the population and economy to floods. The results revealed that the exposed population was approximately 126 persons km-2 per year when taking China as a whole; in terms of the economy, potential losses due to floods were estimated to be approximately 1.49 million C/W4 km 2 and the crop area exposed to floods covered 153 million hm2 per year. China＇s total exposure to floods significantly increased over the analysis period. The areas that showed the higher exposure were mainly located along the east coast. The population＇s vulnerability to floods showed a significantly increasing trend, however, the economic vulnerability showed a decreasing trend. The populations and economies that were most vulnerable to floods were in Hunan, Anhui, Chongqing, Jiangxi, and Hubei provinces. The municipalities of Shanghai, Beijing, and Tianjin showed the lowest vulnerabilities to floods.

关键词： Exposure Vulnerability Flood disaster Spatio-temporal changes China

The Isogene 1-Deoxy-D-Xylulose 5-Phosphate Synthase 2 Controls Isoprenoid Profiles, Precursor Pathway Allocation, and Density of Tomato Trichomes

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Molecular Plant 2010年第5期3卷 904-916页

作者： heike Paetzold Stefan Garms Stefan Bartram Jenny Wieczorek Eva-Maria Uros-Gracia Manuel Rodriguez-Concepcion Wilhelm Boland Dieter Strack Bettina Hause Michael H. Walter Leibniz Institut for Pflanzenbiochemie Abteilung Sekundarstoffwechsel Weinberg 3 D-06120 Halle (Saale) Germany Max-Planck-Institut fur Chemische Okologie Abteilung Bioorganische Chemie Hans-Knoll-Straβe 8 D-07745 Jena Germany Center for Research in Agricultural Genomics (CRAG) CSlC-IRTA-UAB Jordi Girona 18 08034 Barcelona Spain

Plant isoprenoids are formed from precursors synthesized by the mevalonate （MVA） pathway in the cytosol or by the methyl-D-erythritol 4-phosphate （MEP） pathway in plastids. Although some exchange of precursors occurs, cytosolic sesquiterpenes are assumed to derive mainly from MVA, while plastidial monoterpenes are produced preferentially from MEP precursors. Additional complexity arises in the first step of the MEP pathway, which is typically catalyzed by two divergent 1-deoxy-D-xylulose 5-phosphate synthase isoforms （DXS1, DXS2）. In tomato （Solanum lycopersicum）, the SIDXS1 gene is ubiquitously expressed with highest levels during fruit ripening, whereas SIDXS2 transcripts are abundant in only few tissues, including young leaves, petals, and isolated trichomes. Specific down-regulation of SIDXS2 expression was performed by RNA interference in transgenic plants to investigate feedback mechanisms. SIDXS2 down-regulation led to a decrease in the monoterpene β-phellandrene and an increase in two sesquiterpenes in trichomes. Moreover, incorporation of MVA-derived precursors into residual monoterpenes and into sesquiterpenes was elevated as determined by comparison of ^13C to ^12C natural isotope ratios. A compensatory up-regulation of SIDXS1 was not observed. Down-regulated lines also exhibited increased trichome density and showed less damage by leaf-feeding Spodoptera littoralis caterpillars. The results reveal novel, non-redundant roles of DXS2 in modulating isoprenoid metabolism and a pronounced plasticity in isoprenoid precursor allocation.

关键词： Isoprenoid biosynthesis methyI-D-erythritol 4-phosphate (MEP) pathway 1-deoxy-D-xylulose 5-phosphate synthase 2 (DXS2) RNA interference (RNAi) trichomes cross-talk feedback regulation GC-C-IRMS.

Development of innovative tools for investigation of nutrient-gut interaction

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World Journal of Gastroenterology 2020年第25期26卷 3562-3576页

作者： Wei-Kun Huang Cong Xie Richard L Young Jiang-Bo Zhao heike Ebendorff-Heidepriem Karen L Jones Christopher K Rayner Tong-Zhi Wu Adelaide Medical School Centre of Research Excellence in Translating Nutritional Science to Good Healththe University of AdelaideAdelaideSA 5005Australia Institute for Photonics and Advanced Sensing School of Physical SciencesUniversity of AdelaideAdelaideSA 5005Australia The ARC Centre of Excellence for Nanoscale BioPhotonics AdelaideSA 5005Australia Diabetes Nutrition and Gut HealthLifelong HealthSouth Australia Health and Medical Research InstituteAdelaideSA 5005Australia Department of Gastroenterology and Hepatology Royal Adelaide HospitalAdelaideSA 5000Australia Department of Endocrinology Zhongda HospitalInstitute of DiabetesSchool of MedicineSoutheast UniversityNanjing 210009Jiangsu ProvinceChina

The gastrointestinal tract is the key interface between the ingesta and the human *** is wide recognition that the gastrointestinal response to nutrients or bioactive compounds,particularly the secretion of numerous hormones,is critical to the regulation of appetite,body weight and blood *** concept has led to an increasing focus on“gut-based”strategies for the management of metabolic disorders,including type 2 diabetes and *** the underlying mechanisms and downstream effects of nutrient-gut interactions is fundamental to effective translation of this knowledge to clinical *** this end,an array of research tools and platforms have been developed to better understand the mechanisms of gut hormone secretion from enteroendocrine *** review discusses the evolution of in vitro and in vivo models and the integration of innovative techniques that will ultimately enable the development of novel therapies for metabolic diseases.

关键词： Nutrient-gut interaction Metabolic disorders Incretin hormones Enteroendocrine cells Enteroids Intestinal intubation Intestine-on-a-chip

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欧洲刺槐种源群体遗传结构和多样性

生态学报 2004年第12期24卷 2700-2706页

作者：杨敏生 heike Hertel Volker Schneck 河北农业大学保定071000 Institute for Forest Genetics and Forest Tree Breeding

对来自欧洲和美国的 18个刺槐种源子代进行了等位酶分析。可进行遗传分析的 7个酶系统 (Amy,Fe,L ap,Idh,Mdh,6 Pgd,Skd)中有 14个基因位点 ,其中 12个位点具有多态性。每个多态位点平均等位基因数 (A/L )变化在 1.5 6～ 3.6 7之间 ,平... 详细信息

对来自欧洲和美国的 18个刺槐种源子代进行了等位酶分析。可进行遗传分析的 7个酶系统 (Amy,Fe,L ap,Idh,Mdh,6 Pgd,Skd)中有 14个基因位点 ,其中 12个位点具有多态性。每个多态位点平均等位基因数 (A/L )变化在 1.5 6～ 3.6 7之间 ,平均基因型数 (G/L)变化在 1.6 1～ 7.11之间 ,平均等位基因有效数目 (Ae)变化在 1.0 2～ 2 .5 0之间 ,预期杂合度 (H e)变化在0 .0 2～ 0 .5 6之间。不同种源群体之间也存在较大的遗传差异 ,在 8个德国种源中 ,各群体的 A、Ae、和 H e等相对较小 ,但不同群体间差异较大。各位点等位基因频率在不同种源群体间变化也较大 ,表明德国各种源群体内遗传变异相对较小 ,但群体间差异较大。来自匈牙利和斯洛伐克的 8个种源群则相反 ,各群体的 A、Ae、和 H e等相对较大 ,而不同种源群体间差异则较小 ,各位点等位基因频率在种源群体间变化相对一致 ,表明这两个国家的种源群体内变异较大 ,但不同种源群体间差异较小。欧洲的刺槐种源并未形成明显的地理变异模式 ,而且欧洲的种源和来自原产地的美国种源相比 ,没有发现明显的差异。经过 Hardy-Weinberg平衡检测证明 ,88.4 1%位点符合 H- W遗传平衡 ,表明各群体基因频率和基因型频率保持较高的稳定性 ,且种源内的变异大于种源间变异 ,94 .

关键词：刺槐种源群体等位酶遗传结构遗传多样性

Tolerability of a Proprietary Larch Arabinogalactan Extract: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial in Healthy Subjects

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Food and Nutrition Sciences 2012年第11期3卷 1533-1538页

作者： Barbara Grube heike Stier Linda Riede Joerg Gruenwald Analyze & Realize ag Waldseeweg 6 13467 Berlin Germany. Kurfürstendamm 157/158 10709 Berlin Germany

In a placebo-controlled, double-blind, randomized clinical trial, the tolerability of a proprietary larch arabinogalactan preparation (ResistAidTM) was investigated. METHODS: 199 healthy participants were randomly assigned to receive either placebo (n = 98) or an arabinogalactan preparation (n = 101) over a period of 12 weeks. As safety parameters the total number of adverse events, changes in various biochemical and laboratory parameters as well as the global evaluation of tolerability by investigator and subjects compared to placebo were evaluated. RESULTS: In total 16 adverse events were observed in 16 subjects, with no difference between the arabinogalactan and the placebo group (p = 0.935). There were no differences in the mean changes of the measured biochemical and laboratory parameters. The tolerability of the arabinogalactan extract was rated as “very good” or “good” by the investigators for 99% of the subjects and by 98% subjects in self-assessment with no statistical differences to placebo. CONCLUSION: The measured parameters as well as the evaluation of the tolerability by the investigators and the subjects demonstrate a very good tolerance profile of the proprietary arabinogalactan extract with no differences to placebo when taken for 12 weeks.

关键词： Randomized Placebo-Controlled Study Proprietary Larch Arabinogalactan Safety Tolerability Resist AidTM

Corncobs as Substrate for Oleaginous Yeast-Pretreatment via Steam Explosion and Hydrolysis

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Journal of Environmental Science and Engineering(A) 2015年第7期4卷 359-366页

作者： heike Kahr Manuel Pointner KlausKrennhuber Daniel Steindl AlexanderJager Department of Research and Development University of Applied Sciences Upper Austria Wels 4600 Austria

Corn cobs are a promising lignocellulosic substrate for the production of biofuels like bioethanol via conventional yeast or biodiesel via oleaginous yeast. Pretreatment of the substrate is essential for further hydrolysis and fermentation steps. This study focused on the steam explosion method as pretreatment. Therefore, different steam explosion severities were evaluated. The content of glucan, xylan and Klason lignin was examined. Xylan degraded with increasing severity from 412.7 g·kg-1 （untreated） to a minimum of 127.3 g-kg1 dry matter （190 ℃/30 min）. Glucan concentrations increased from 315.1 g·kg1 （untreated） to a maximum of 371.6 g·kg-1 dry matter （200 ℃/20 min）. For soluble lignin, an increase could be observed at rising severity, from 145.3 g·kg-l （untreated） to a maximum of 214.9 g·kg-1 dry matter （190 ℃/30 min）. Furthermore, the mass recovery was calculated. At harsher pretreatment conditions, a significant mass loss was observed, estimated by the ash content in the recovered dry matter. The lowest recovery rate was observed for SF = 4.13 （190 ℃/30 min） with 68.39%. The produced inhibitors were evaluated.

关键词： Corn cobs steam explosion lignocellulose pretreatment.

Controlled formation of gold nanoparticles with tunable plasmonic properties in tellurite glass

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Light(Science & Applications) 2023年第12期12卷 2788-2801页

作者： Yunle Wei Jiangbo Zhao Sindy Fuhrmann Roman Sajzew Lothar Wondraczek heike Ebendorff-Heidepriem Institute for Photonics and Advanced Sensing School of PhysicsChemistry and Earth SciencesThe University of AdelaideAdelaideSA 5005Australia School of Engineering University of HullHull HU67RXUK Institute of Glass Science and Technology TU Bergakademie Freiberg09599 FreibergGermany Otto Schott Institute of Materials Research University of Jena07743 JenaGermany Leibniz Institute of Photonic Technology 07745 JenaGermany Center of Energy and Environmental Chemistry University of Jena07743 JenaGermany

Silicate glasses with metallic nanoparticles(NPs)have been of intense interest in art,science and technology as the plasmonic properties of these NPs equip glass with light modulation *** so-called striking technique has enabled precise control of the in situ formation of metallic NPs in silicate glasses for applications from coloured glasses to photonic *** tellurite glasses exhibit the unique combination of comparably easy fabrication,low phonon energy,wide transmission window and high solubility of luminescent rare earth ions,there has been a significant amount of work over the past two decades to adapt the striking technique to form gold or silver NPs in tellurite *** this effort,the striking technique has remained insufficient for tellurite glasses to form metal NPs suitable for photonic ***,we first uncover the challenges of the traditional striking technique to create gold NPs in tellurite ***,we demonstrate precise control of the size and concentration of gold NPs in tellurite glass by developing new approaches to both steps of the striking technique:a controlled gold crucible corrosion technique to incorporate gold ions in tellurite glass and a glass powder reheating technique to subsequently transform the gold ions to gold *** the Mie theory,the size,size distribution and concentration of the gold NPs formed in tellurite glass are determined from the plasmonic properties of the *** fundamental research provides guidance for designing and manipulating the plasmonic properties in tellurite glass for photonics research and applications.

关键词： tunable glasses glass