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Bond-selective transient phase imaging via sensing of the infrared photothermal effect

Light(Science & Applications) 2019年第1期8卷 70-81页

作者： Delong Zhang Lu Lan Yeran Bai hassaan majeed Mikhail E.Kandel Gabriel Popescu Ji-Xin Cheng Department of Biomedical Engineering Boston UniversityBostonMA 02215USA Department of Physics Zhejiang UniversityHangzhou 310028China National Laboratory on High Power Laser and Physics Shanghai 201800China Key Laboratory of High Power Laser and Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Department of Bioengineering University of Illinois at Urbana-ChampaignChampaignIL 61801USA Department of Electrical and Computer Engineering University of Illinois at Urbana-ChampaignChampaignIL 61801USA Department of Electrical&Computer Engineering Boston UniversityBostonMA 02215USA Photonics Center Boston UniversityBostonMA 02215USA

Phase-contrast microscopy converts the phase shift of light passing through a transparent specimen,e.g.,a biological cell,into brightness variations in an *** ability to observe structures without destructive fixation or staining has been widely utilized for applications in materials and life *** these advantages,phase-contrast microscopy lacks the ability to reveal molecular *** address this gap,we developed a bond-selective transient phase(BSTP)imaging technique that excites molecular vibrations by infrared light,resulting in a transient change in phase shift that can be detected by a diffraction phase *** developing a time-gated pump-probe camera system,we demonstrate BSTP imaging of live cells at a 50 Hz frame rate with high spectral fidelity,sub-microsecond temporal resolution,and sub-micron spatial *** approach paves a new way for spectroscopic imaging investigation in biology and materials science.

关键词： transient phase utilized

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High-resolution impedance mapping using electrically activated quantitative phase imaging

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Light(Science & Applications) 2021年第2期10卷 186-196页

作者： Cristina Polonschii Mihaela Gheorghiu Sorin David Szilveszter Gáspár Sorin Melinte hassaan majeed Mikhail E.Kandel Gabriel Popescu Eugen Gheorghiu International Centre of Biodynamics 060101 BucharestRomania Institute of Information and Communication Technologies Electronics and Applied MathematicsUniversite Catholique de Louvain1348 Louvain-la-NeuveBelgium Quantitative Light Imaging Laboratory Beckman Institute for Advanced Science and TechnologyUniversity of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Retrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need,likely to impact various applications from biomedicine to energy *** this study,we develop a multimodal functional imaging instrument that is characterized by the dual capability of impedance mapping and phase quantitation,high spatial resolution,and low temporal *** achieve this,we advance a quantitative phase imaging system,referred to as epi-magnified image spatial spectrum microscopy combined with electrical actuation,to provide complementary maps of the optical path and electrical *** demonstrate our system with high-resolution maps of optical path differences and electrical impedance variations that can distinguish nanosized,semi-transparent,structured coatings involving two materials with relatively similar electrical *** map heterogeneous interfaces corresponding to an indium tin oxide layer exposed by holes with diameters as small as~550 nm in a titanium(dioxide)over-layer deposited on a glass *** show that electrical modulation during the phase imaging of a macro-electrode is decisive for retrieving electrical impedance distributions with submicron spatial resolution-and beyond the limitations of electrode-based technologies(surface or scanning technologies).The findings,which are substantiated by a theoretical model that fits the experimental data very well enable achieving electro-optical maps with high spatial and temporal *** virtues and limitations of the novel optoelectrochemical method that provides grounds for a wider range of electrically modulated optical methods for measuring the electric field locally are critically discussed.

关键词： impedance resolution phase

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